(Circular) (Six-base) MAPSORT of: pFN21AB7335.seq Check: 8522 from: 1 to: 7765 pFN21AB7335 7765 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2038 4964 2038 Size: 2926 4839 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5575 278 Size: 53 83 186 936 4039 2468 Fragments arranged by size: 4039 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3024 5108 1937 Size: 1087 2084 4594 Fragments arranged by size: 4594 2084 1087 Acc65I G'GTAC_C Cuts at: 2248 5086 2248 Size: 2838 4927 AclI AA'CG_TT Cuts at: 1563 5892 6265 1563 Size: 4329 373 3063 Fragments arranged by size: 4329 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 3518 4494 4509 4602 5828 7212 7480 Size: 512 1374 976 15 93 1226 1384 268 Cuts at: 7480 1632 Size: 1917 Fragments arranged by size: 1917 1384 1374 1226 976 512 268 93 15 AfeI AGC'GCT Cuts at: 4394 4394 Size: 7765 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7568 AflIII A'CryG_T Cuts at: 1203 6664 7641 1203 Size: 5461 977 1327 Fragments arranged by size: 5461 1327 977 AgeI A'CCGG_T Cuts at: 6596 6596 Size: 7765 AhdI GACnn_n'nnGTC Cuts at: 6494 6494 Size: 7765 AleI CACnn'nnGTG Cuts at: 1166 3629 4596 4724 1166 Size: 2463 967 128 4207 Fragments arranged by size: 4207 2463 967 128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7765 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2211 2243 2211 Size: 32 7733 AlwNI CAG_nnn'CTG Cuts at: 2506 3554 4201 4792 5508 7080 2506 Size: 1048 647 591 716 1572 3191 Fragments arranged by size: 3191 1572 1048 716 647 591 ApaI G_GGCC'C Cuts at: 3365 3365 Size: 7765 ApaLI G'TGCA_C Cuts at: 3429 5822 6978 3429 Size: 2393 1156 4216 Fragments arranged by size: 4216 2393 1156 ApoI r'AATT_y Cuts at: 1493 3715 5074 5300 5452 7561 1493 Size: 2222 1359 226 152 2109 1697 Fragments arranged by size: 2222 2109 1697 1359 226 152 AseI AT'TA_AT Cuts at: 160 1032 2939 6319 160 Size: 872 1907 3380 1606 Fragments arranged by size: 3380 1907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7765 AvaI C'yCGr_G Cuts at: 1870 1943 2259 3366 3799 5090 1870 Size: 73 316 1107 433 1291 4545 Fragments arranged by size: 4545 1291 1107 433 316 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2793 2826 4865 4898 2793 Size: 33 2039 33 5660 Fragments arranged by size: 5660 2039 33 33 BamHI G'GATC_C Cuts at: 5095 5462 5095 Size: 367 7398 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2248 3187 3337 4288 5086 Size: 325 217 627 461 939 150 951 798 Cuts at: 5086 6541 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 951 939 798 627 461 325 217 150 BanII G_rGCy'C Cuts at: 1391 3365 4710 5084 1391 Size: 1974 1345 374 4072 Fragments arranged by size: 4072 1974 1345 374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2646 928 Size: 971 747 6047 Fragments arranged by size: 6047 971 747 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3428 3462 3725 3759 5956 5990 1903 Size: 34 1491 34 263 34 2197 34 3678 Fragments arranged by size: 3678 2197 1491 263 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5657 6867 5657 Size: 1210 6555 BclI T'GATC_A Cuts at: 1548 2787 1548 Size: 1239 6526 BfrBI ATG'CAT Cuts at: 3742 3987 3742 Size: 245 7520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6376 136 Size: 107 122 71 631 605 4704 1525 Fragments arranged by size: 4704 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2349 2448 7760 2349 Size: 99 5312 2354 Fragments arranged by size: 5312 2354 99 BlpI GC'TnA_GC Cuts at: 4345 4856 5182 4345 Size: 511 326 6928 Fragments arranged by size: 6928 511 326 Bme1580I G_kGCm'C Cuts at: 1790 2928 3365 3433 3931 4291 5826 6982 1790 Size: 1138 437 68 498 360 1535 1156 2573 Fragments arranged by size: 2573 1535 1156 1138 498 437 360 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7765 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2678 3642 3667 Size: 416 53 83 399 24 1261 964 25 Cuts at: 3667 5496 6454 442 Size: 1829 958 1753 Fragments arranged by size: 1829 1753 1261 964 958 416 399 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7765 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 4717 3614 Size: 1103 6662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3371 3597 3626 3782 3895 3907 4559 4712 Size: 2132 226 29 156 113 12 652 153 Cuts at: 4712 4769 5003 6425 1239 Size: 57 234 1422 2579 Fragments arranged by size: 2579 2132 1422 652 234 226 156 153 113 57 29 12 Bpu10I CC'TnA_GC Cuts at: 2309 2389 2991 3555 2309 Size: 80 602 564 6519 Fragments arranged by size: 6519 602 564 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 5890 6755 7053 4075 Size: 1815 865 298 4787 Fragments arranged by size: 4787 1815 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3133 4930 6428 882 Size: 617 1634 1797 1498 2219 Fragments arranged by size: 2219 1797 1634 1498 617 BsaAI yAC'GTr Cuts at: 493 1204 5556 493 Size: 711 4352 2702 Fragments arranged by size: 4352 2702 711 BsaBI GATnn'nnATC Cuts at: 7759 7759 Size: 7765 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2685 4364 5572 5954 Size: 53 83 186 936 1152 1679 1208 382 Cuts at: 5954 6589 275 Size: 635 1451 Fragments arranged by size: 1679 1451 1208 1152 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6198 6596 6870 7017 7428 6198 Size: 398 274 147 411 6535 Fragments arranged by size: 6535 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 4619 4649 4727 Size: 30 165 30 936 30 2250 30 78 Cuts at: 4727 4757 1178 Size: 30 4186 Fragments arranged by size: 4186 2250 936 165 78 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2464 2904 3032 3535 3838 4448 4962 1186 Size: 1278 440 128 503 303 610 514 3989 Fragments arranged by size: 3989 1278 610 514 503 440 303 128 BseYI C'CCAG_C Cuts at: 2042 2535 4183 4452 6968 7464 2042 Size: 493 1648 269 2516 496 2343 Fragments arranged by size: 2516 2343 1648 496 493 269 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 2153 2793 3719 3853 1163 Size: 573 369 48 640 926 134 5075 Fragments arranged by size: 5075 926 640 573 369 134 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5215 5976 7004 1540 Size: 275 186 3214 761 1028 2301 Fragments arranged by size: 3214 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3123 3433 3636 4710 5826 5911 6982 3123 Size: 310 203 1074 1116 85 1071 3906 Fragments arranged by size: 3906 1116 1074 1071 310 203 85 BsiWI C'GTAC_G Cuts at: 3217 3217 Size: 7765 BsmI GAATG_Cn' Cuts at: 5276 5369 5276 Size: 93 7672 BsmBI CGTCTCn'nnnn_ Cuts at: 2692 2692 Size: 7765 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2928 3123 3365 3433 3636 3931 4291 Size: 399 1138 195 242 68 203 295 360 Cuts at: 4291 4710 5084 5826 5911 6982 1391 Size: 419 374 742 85 1071 2174 Fragments arranged by size: 2174 1138 1071 742 419 399 374 360 295 242 203 195 85 68 BspEI T'CCGG_A Cuts at: 7428 7428 Size: 7765 BspHI T'CATG_A Cuts at: 5654 7384 5654 Size: 1730 6035 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2038 4964 5120 844 Size: 1194 2926 156 3489 Fragments arranged by size: 3489 2926 1194 156 BsrBI CCG'CTC Cuts at: 1394 1823 3588 4344 5652 1394 Size: 429 1765 756 1308 3507 Fragments arranged by size: 3507 1765 1308 756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2961 3814 4446 6260 6434 65 Size: 1183 1713 853 632 1814 174 1396 Fragments arranged by size: 1814 1713 1396 1183 853 632 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4027 4209 6409 6596 1700 Size: 110 2217 182 2200 187 2869 Fragments arranged by size: 2869 2217 2200 187 182 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7765 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7765 BssSI C'ACGA_G Cuts at: 5825 6837 5825 Size: 1012 6753 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7765 BstBI TT'CG_AA Cuts at: 6569 6569 Size: 7765 BstEII G'GTnAC_C Cuts at: 1184 2263 2800 1184 Size: 1079 537 6149 Fragments arranged by size: 6149 1079 537 BstXI CCAn_nnnn'nTGG Cuts at: 3650 3650 Size: 7765 BstYI r'GATC_y Cuts at: 1972 2349 2423 2448 3837 4065 4806 4968 5095 Size: 377 74 25 1389 228 741 162 127 Cuts at: 5095 5462 5847 5864 7316 7402 7414 7760 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1389 741 385 377 367 346 228 162 127 86 74 25 17 12 Bsu36I CC'TnA_GG Cuts at: 3357 3357 Size: 7765 BtgI C'CryG_G Cuts at: 513 1065 2004 2691 3705 4826 513 Size: 552 939 687 1014 1121 3452 Fragments arranged by size: 3452 1121 1014 939 687 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2935 3728 3742 487 Size: 683 56 1709 793 14 4510 Fragments arranged by size: 4510 1709 793 683 56 14 BtsI GCAGTG_nn' Cuts at: 2744 2834 2860 3067 4322 4516 5281 6075 6095 Size: 90 26 207 1255 194 765 794 20 Cuts at: 6095 2744 Size: 4414 Fragments arranged by size: 4414 1255 794 765 207 194 90 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7765 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4311 4346 529 Size: 35 3747 35 3948 Fragments arranged by size: 3948 3747 35 35 DraI TTT'AAA Cuts at: 5069 5069 Size: 7765 DraIII CAC_nnn'GTG Cuts at: 2503 2503 Size: 7765 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4958 6772 809 Size: 507 3642 1814 1802 Fragments arranged by size: 3642 1814 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2595 2917 3258 Size: 54 1425 50 275 18 765 322 341 Cuts at: 3258 5212 6101 7730 8 Size: 1954 889 1629 43 Fragments arranged by size: 1954 1629 1425 889 765 341 322 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5212 1537 Size: 275 3400 4090 Fragments arranged by size: 4090 3400 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3605 4056 4357 4578 4622 5695 1353 Size: 2252 451 301 221 44 1073 3423 Fragments arranged by size: 3423 2252 1073 451 301 221 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6352 6726 6872 387 Size: 1064 138 4763 374 146 1280 Fragments arranged by size: 4763 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4708 4708 Size: 7765 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 3371 3518 3597 3626 3782 3895 Size: 393 512 1227 147 79 29 156 113 Cuts at: 3895 3907 4494 4509 4559 4602 4712 4769 5003 Size: 12 587 15 50 43 110 57 234 Cuts at: 5003 5828 6425 7212 7480 1239 Size: 825 597 787 268 1524 Fragments arranged by size: 1524 1227 825 787 597 587 512 393 268 234 156 147 113 110 79 57 50 43 29 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 2582 5056 2582 Size: 2474 5291 EcoO109I rG'GnC_Cy Cuts at: 2662 2970 3270 3361 3362 3979 4198 4687 4757 Size: 308 300 91 1 617 219 489 70 Cuts at: 4757 4789 4901 2662 Size: 32 112 5526 Fragments arranged by size: 5526 617 489 308 300 219 112 91 70 32 1 EcoRI G'AATT_C Cuts at: 5074 5452 5074 Size: 378 7387 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4291 4499 4291 Size: 208 7557 FspI TGC'GCA Cuts at: 3059 3317 6271 3059 Size: 258 2954 4553 Fragments arranged by size: 4553 2954 258 FspAI rTGC'GCAy Cuts at: 3317 3317 Size: 7765 HaeII r_GCGC'y Cuts at: 1678 2020 3817 4396 6912 1678 Size: 342 1797 579 2516 2531 Fragments arranged by size: 2531 2516 1797 579 342 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2415 2447 2689 2721 4637 Size: 32 135 32 843 32 242 32 1916 Cuts at: 4637 4669 6406 6438 6480 6512 1373 Size: 32 1737 32 42 32 2626 Fragments arranged by size: 2626 1916 1737 843 242 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5109 5355 669 Size: 988 281 3171 246 3079 Fragments arranged by size: 3171 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7765 HpaI GTT'AAC Cuts at: 5355 5355 Size: 7765 KpnI G_GTAC'C Cuts at: 2252 5090 2252 Size: 2838 4927 MfeI C'AATT_G Cuts at: 3275 5364 3275 Size: 2089 5676 MluI A'CGCG_T Cuts at: 7641 7641 Size: 7765 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2283 4504 6879 7063 1305 Size: 978 2221 2375 184 2007 Fragments arranged by size: 2375 2221 2007 978 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2597 2919 3260 10 Size: 54 1425 343 765 322 341 4515 Fragments arranged by size: 4515 1425 765 343 341 322 54 MslI CAynn'nnrTG Cuts at: 518 1166 2954 3629 4529 4596 4724 5723 6082 Size: 648 1788 675 900 67 128 999 359 Cuts at: 6082 6241 518 Size: 159 2042 Fragments arranged by size: 2042 1788 999 900 675 648 359 159 128 67 MspA1I CmG'CkG Cuts at: 1606 2385 2539 2702 4580 4879 5181 5858 7006 Size: 779 154 163 1878 299 302 677 1148 Cuts at: 7006 7251 1606 Size: 245 2120 Fragments arranged by size: 2120 1878 1148 779 677 302 299 245 163 154 NcoI C'CATG_G Cuts at: 513 1065 2004 2691 3705 513 Size: 552 939 687 1014 4573 Fragments arranged by size: 4573 1014 939 687 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7765 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7765 NotI GC'GGCC_GC Cuts at: 5212 5212 Size: 7765 NsiI A_TGCA'T Cuts at: 3744 3989 3744 Size: 245 7520 NspI r_CATG'y Cuts at: 1132 1229 3316 3742 5123 6668 1132 Size: 97 2087 426 1381 1545 2229 Fragments arranged by size: 2229 2087 1545 1381 426 97 PasI CC'CwG_GG Cuts at: 1348 2201 1348 Size: 853 6912 PciI A'CATG_T Cuts at: 6664 6664 Size: 7765 PflMI CCAn_nnn'nTGG Cuts at: 1108 2321 2506 4745 1108 Size: 1213 185 2239 4128 Fragments arranged by size: 4128 2239 1213 185 PfoI T'CCnGG_A Cuts at: 3759 4785 3759 Size: 1026 6739 PmeI GTTT'AAAC Cuts at: 5069 5069 Size: 7765 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5834 5866 7347 7379 5834 Size: 32 1481 32 6220 Fragments arranged by size: 6220 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2662 2970 3270 4757 4789 4901 2662 Size: 308 300 1487 32 112 5526 Fragments arranged by size: 5526 1487 308 300 112 32 PshAI GACnn'nnGTC Cuts at: 1613 2491 1613 Size: 878 6887 PsiI TTA'TAA Cuts at: 5335 5335 Size: 7765 PspOMI G'GGCC_C Cuts at: 3361 3361 Size: 7765 PspXI vC'TCGA_Gb Cuts at: 3366 3366 Size: 7765 PstI C_TGCA'G Cuts at: 830 2493 2556 2610 2751 2912 3498 4511 4579 Size: 1663 63 54 141 161 586 1013 68 Cuts at: 4579 5117 830 Size: 538 3478 Fragments arranged by size: 3478 1663 1013 586 538 161 141 68 63 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7765 PvuII CAG'CTG Cuts at: 2702 4580 4879 2702 Size: 1878 299 5588 Fragments arranged by size: 5588 1878 299 SacI G_AGCT'C Cuts at: 4710 4710 Size: 7765 SalI G'TCGA_C Cuts at: 1936 5107 1936 Size: 3171 4594 SanDI GG'GwC_CC Cuts at: 3270 3270 Size: 7765 SbfI CC_TGCA'GG Cuts at: 5117 5117 Size: 7765 ScaI AGT'ACT Cuts at: 6013 7725 6013 Size: 1712 6053 SexAI A'CCwGG_T Cuts at: 2499 3123 4315 2499 Size: 624 1192 5949 Fragments arranged by size: 5949 1192 624 SfcI C'TryA_G Cuts at: 826 1045 2489 2552 2606 2747 2908 3235 3494 Size: 219 1444 63 54 141 161 327 259 Cuts at: 3494 3817 4507 4575 5113 6248 6929 7120 826 Size: 323 690 68 538 1135 681 191 1471 Fragments arranged by size: 1471 1444 1135 690 681 538 327 323 259 219 191 161 141 68 63 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7765 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7765 SmaI CCC'GGG Cuts at: 1872 2261 3801 5092 1872 Size: 389 1540 1291 4545 Fragments arranged by size: 4545 1540 1291 389 SmlI C'TyrA_G Cuts at: 820 1017 1943 3366 4054 5869 6770 7032 820 Size: 197 926 1423 688 1815 901 262 1553 Fragments arranged by size: 1815 1553 1423 926 901 688 262 197 SnaBI TAC'GTA Cuts at: 493 5556 493 Size: 5063 2702 SpeI A'CTAG_T Cuts at: 152 3889 152 Size: 3737 4028 SphI G_CATG'C Cuts at: 1132 5123 1132 Size: 3991 3774 SspI AAT'ATT Cuts at: 5 52 3086 5689 5 Size: 47 3034 2603 2081 Fragments arranged by size: 3034 2603 2081 47 StuI AGG'CCT Cuts at: 1517 2468 1517 Size: 951 6814 StyI C'CwwG_G Cuts at: 513 1065 2004 2231 2691 3705 3975 4116 4275 Size: 552 939 227 460 1014 270 141 159 Cuts at: 4275 4329 5204 513 Size: 54 875 3074 Fragments arranged by size: 3074 1014 939 875 552 460 270 227 159 141 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2193 4720 4839 5792 5809 5962 6147 Size: 1255 613 2527 119 953 17 153 185 Cuts at: 6147 6592 325 Size: 445 1498 Fragments arranged by size: 2527 1498 1255 953 613 445 185 153 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4608 6011 7723 Size: 274 80 33 51 1443 2631 1403 1712 Cuts at: 7723 96 Size: 138 Fragments arranged by size: 2631 1712 1443 1403 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3521 3553 4619 4651 7725 7757 3521 Size: 32 1066 32 3074 32 3529 Fragments arranged by size: 3529 3074 1066 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3200 1316 Size: 1884 5881 XbaI T'CTAG_A Cuts at: 5101 5101 Size: 7765 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2852 2852 Size: 7765 XhoI C'TCGA_G Cuts at: 1943 3366 1943 Size: 1423 6342 XmaI C'CCGG_G Cuts at: 1870 2259 3799 5090 1870 Size: 389 1540 1291 4545 Fragments arranged by size: 4545 1540 1291 389 XmnI GAAnn'nnTTC Cuts at: 5894 5894 Size: 7765 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5573 276 Size: 53 83 186 936 4039 2468 Fragments arranged by size: 4039 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BbvCI BstZ17I EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI