################################## # NO CUT ENZYMES ( 218 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### BbrPI 248 CACGTG CAC^GTG BfaI 299 CTAG C^TAG BglII 306 AGATCT A^GATCT BsaAI 248 CACGTG [CT]AC^GT[GA] BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG BseRI 349 CTCCTC GAGGAG(10/8) BsmAI 223 GAGAC GTCTC(1/5) BsmFI 45 GGGAC GGGAC(10/14) BssKI 42 CCTGG ^CC[ACGT]GG BstNI 42 CCTGG CC^[AT]GG BstYI 306 AGATCT [GA]^GATC[CT] DpnII 307 GATC ^GATC EcoRII 42 CCTGG ^CC[AT]GG HinfI 172 GAGTC G^A[ACGT]TC MaeI 299 CTAG C^TAG MaeII 249 ACGT A^CGT MboI 307 GATC ^GATC MboII 64 GAAGA GAAGA(8/7) MflI 306 AGATCT [GA]^GATC[CT] MvaI 42 CCTGG CC^[AT]GG MwoI 19 GCCATCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 307 GATC ^GATC NlaIII 390 CATG CATG^ NlaIV 294 GGAACC GG[ACGT]^[ACGT]CC PleI 172 GAGTC GAGTC(4/5) PmaCI 248 CACGTG CAC^GTG PmlI 248 CACGTG CAC^GTG Sau3AI 307 GATC ^GATC ScrFI 42 CCTGG CC^[ACGT]GG SfaNI 316 GATGC GCATC(5/9) SspI 86 AATATT AAT^ATT Tsp45I 115 GTCAC ^GT[GC]AC XhoII 306 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 2 enzymes) # ################################### BsgI 140 GTGCAG GTGCAG(16/14) 251 GTGCAG HphI 116 TCACC GGTGA(8/7) 149 TCACC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### BsrI BstF5I FokI MaeIII MnlI