Restriction map for
pFN21AE0441
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII HphI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacII SalI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AciI 123 GCGG CCGC(-3/-1)
AcsI 256 GAATTT [GA]^AATT[CT]
AocI 605 CCTCAGG CC^T[ACGT]AGG
ApoI 256 GAATTT [GA]^AATT[CT]
BamHI 513 GGATCC G^GATCC
BanI 483 GGCACC G^G[CT][GA]CC
BbsI 474 GAAGAC GAAGAC(2/6)
BglI 7 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 474 GAAGAC GAAGAC(2/6)
BspMI 535 ACCTGC ACCTGC(4/8)
BssKI 63 CCTGG ^CC[ACGT]GG
BstAPI 454 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 63 CCTGG CC^[AT]GG
BstXI 419 CCAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 605 CCTCAGG CC^T[ACGT]AGG
Cac8I 669 GCCTGC GC[ACGT]^[ACGT]GC
EaeI 5 CGGCCA [CT]^GGCC[GA]
Eco81I 605 CCTCAGG CC^T[ACGT]AGG
EcoRII 63 CCTGG ^CC[AT]GG
HindIII 337 AAGCTT A^AGCTT
MvaI 63 CCTGG CC^[AT]GG
SacI 184 GAGCTC GAGCT^C
ScrFI 63 CCTGG CC^[ACGT]GG
SmlI 187 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AvaII 150 GGACC G^G[AT]CC
533 GGACC
BanII 184 GAGCTC G[GA]GC[CT]^C
504 GGGCTC
BbvI 366 GCAGC GCAGC(8/12)
539 GCTGC
BseRI 202 GAGGAG GAGGAG(10/8)
628 GAGGAG
BsiYI 649 CCTTCTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
684 CCAGACTGAGG
BslI 649 CCTTCTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
684 CCAGACTGAGG
BsmFI 532 GGGAC GGGAC(10/14)
681 GTCCC
BstF5I 294 GGATG GGATG(2/0)
429 GGATG
BstYI 222 GGATCT [GA]^GATC[CT]
513 GGATCC
DpnII 223 GATC ^GATC
514 GATC
DraII 149 AGGACCT [GA]G^G[ACGT]CC[CT]
532 GGGACCT
DraIII 455 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
661 CACGCTGTG
EcoO109I 149 AGGACCT [GA]G^G[ACGT]CC[CT]
532 GGGACCT
Fnu4HI 366 GCAGC GC^[ACGT]GC
539 GCTGC
FokI 294 GGATG GGATG(9/13)
429 GGATG
HinfI 393 GACTC G^A[ACGT]TC
443 GAGTC
ItaI 366 GCAGC GC^[ACGT]GC
539 GCTGC
MaeIII 142 GTGAC ^GT[ACGT]AC
637 GTGAC
MboI 223 GATC ^GATC
514 GATC
MflI 222 GGATCT [GA]^GATC[CT]
513 GGATCC
MseI 110 TTAA T^TAA
313 TTAA
MslI 32 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
523 CATCACTGTG
NdeII 223 GATC ^GATC
514 GATC
PleI 393 GACTC GAGTC(4/5)
443 GAGTC
PpuMI 149 AGGACCT [GA]G^G[AT]CC[CT]
532 GGGACCT
Sau3AI 223 GATC ^GATC
514 GATC
StuI 602 AGGCCT AGG^CCT
609 AGGCCT
Tru9I 110 TTAA T^TAA
313 TTAA
TseI 366 GCAGC G^C[AT]GC
539 GCTGC
Tsp45I 142 GTGAC ^GT[GC]AC
637 GTGAC
XhoII 222 GGATCT [GA]^GATC[CT]
513 GGATCC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI AlwI AspHI BmyI BsiHKAI
Bsp1286I BsrI Cfr13I DdeI EarI
HaeIII Ksp632I MboII MnlI MwoI
NlaIV SapI Sau96I Tsp509I TspRI