(Circular) (Six-base) MAPSORT of: pFN21AE0046.seq Check: 4806 from: 1 to: 6622 pFN21AE0046 6622 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3540 3540 Size: 6622 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3965 1937 Size: 2028 4594 Acc65I G'GTAC_C Cuts at: 2739 3943 2739 Size: 1204 5418 AclI AA'CG_TT Cuts at: 1563 4749 5122 1563 Size: 3186 373 3063 Fragments arranged by size: 3186 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 3190 3478 4685 6069 6337 1632 Size: 419 1139 288 1207 1384 268 1917 Fragments arranged by size: 1917 1384 1207 1139 419 288 268 AfeI AGC'GCT Cuts at: 3380 3380 Size: 6622 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 3759 5521 6498 1203 Size: 2556 1762 977 1327 Fragments arranged by size: 2556 1762 1327 977 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 3124 5351 3124 Size: 2227 4395 AleI CACnn'nnGTG Cuts at: 1166 3266 1166 Size: 2100 4522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6622 AlwNI CAG_nnn'CTG Cuts at: 2652 4365 5937 2652 Size: 1713 1572 3337 Fragments arranged by size: 3337 1713 1572 ApaI G_GGCC'C Cuts at: 2236 3168 2236 Size: 932 5690 ApaLI G'TGCA_C Cuts at: 4679 5835 4679 Size: 1156 5466 ApoI r'AATT_y Cuts at: 1493 3931 4157 4309 6418 1493 Size: 2438 226 152 2109 1697 Fragments arranged by size: 2438 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 2229 3620 3947 1870 Size: 73 286 1391 327 4545 Fragments arranged by size: 4545 1391 327 286 73 BamHI G'GATC_C Cuts at: 2901 3952 4319 2901 Size: 1051 367 5204 Fragments arranged by size: 5204 1051 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2628 2739 2851 3268 3560 Size: 325 217 627 841 111 112 417 292 Cuts at: 3560 3626 3719 3943 5398 618 Size: 66 93 224 1455 1842 Fragments arranged by size: 1842 1455 841 627 417 325 292 224 217 112 111 93 66 BanII G_rGCy'C Cuts at: 1391 2236 2330 2806 3168 3465 3876 3941 1391 Size: 845 94 476 362 297 411 65 4072 Fragments arranged by size: 4072 845 476 411 362 297 94 65 BbeI G_GCGC'C Cuts at: 3630 3630 Size: 6622 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2587 928 Size: 971 688 4963 Fragments arranged by size: 4963 971 688 BbvCI CC'TCA_GC Cuts at: 2021 3169 2021 Size: 1148 5474 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4813 4847 1903 Size: 34 2876 34 3678 Fragments arranged by size: 3678 2876 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4514 5724 4514 Size: 1210 5412 BclI T'GATC_A Cuts at: 1548 1548 Size: 6622 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3639 5233 136 Size: 107 122 71 631 605 1967 1594 1525 Fragments arranged by size: 1967 1594 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2389 6617 2389 Size: 4228 2394 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 2082 2236 3168 4683 5839 1790 Size: 292 154 932 1515 1156 2573 Fragments arranged by size: 2573 1515 1156 932 292 154 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2529 4353 5311 Size: 416 53 83 399 24 1112 1824 958 Cuts at: 5311 442 Size: 1753 Fragments arranged by size: 1824 1753 1112 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 6622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2483 2889 3668 3815 5282 1239 Size: 1244 406 779 147 1467 2579 Fragments arranged by size: 2579 1467 1244 779 406 147 Bpu10I CC'TnA_GC Cuts at: 2021 3169 2021 Size: 1148 5474 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2685 4747 5612 5910 2685 Size: 2062 865 298 3397 Fragments arranged by size: 3397 2062 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5285 882 Size: 617 3786 2219 Fragments arranged by size: 3786 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4413 493 Size: 711 3209 2702 Fragments arranged by size: 3209 2702 711 BsaBI GATnn'nnATC Cuts at: 2388 6616 2388 Size: 4228 2394 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2592 3627 3653 4429 Size: 53 83 186 936 1059 1035 26 776 Cuts at: 4429 4811 5446 275 Size: 382 635 1451 Fragments arranged by size: 1451 1059 1035 936 776 635 382 186 83 53 26 BsaWI w'CCGG_w Cuts at: 2756 2898 5055 5453 5727 5874 6285 2756 Size: 142 2157 398 274 147 411 3093 Fragments arranged by size: 3093 2157 411 398 274 147 142 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3227 3257 3746 3776 1178 Size: 30 165 30 1824 30 489 30 4024 Fragments arranged by size: 4024 1824 489 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2814 3050 3443 1186 Size: 1628 236 393 4365 Fragments arranged by size: 4365 1628 393 236 BseYI C'CCAG_C Cuts at: 3486 5825 6321 3486 Size: 2339 496 3787 Fragments arranged by size: 3787 2339 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 1163 Size: 573 450 5599 Fragments arranged by size: 5599 573 450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3276 3826 4072 4833 5861 1540 Size: 275 186 1275 550 246 761 1028 2301 Fragments arranged by size: 2301 1275 1028 761 550 275 246 186 BsiHKAI G_wGCw'C Cuts at: 2586 4683 4768 5839 2586 Size: 2097 85 1071 3369 Fragments arranged by size: 3369 2097 1071 85 BsmI GAATG_Cn' Cuts at: 4133 4226 4133 Size: 93 6529 BsmBI CGTCTCn'nnnn_ Cuts at: 3644 3644 Size: 6622 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2236 2330 2586 2806 3168 3465 Size: 399 292 154 94 256 220 362 297 Cuts at: 3465 3876 3941 4683 4768 5839 1391 Size: 411 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 411 399 362 297 292 256 220 154 94 85 65 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 2380 4511 6241 2380 Size: 2131 1730 2761 Fragments arranged by size: 2761 2131 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3540 3840 3977 844 Size: 2696 300 137 3489 Fragments arranged by size: 3489 2696 300 137 BsrBI CCG'CTC Cuts at: 1394 1823 4509 1394 Size: 429 2686 3507 Fragments arranged by size: 3507 2686 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5117 5291 65 Size: 1183 3869 174 1396 Fragments arranged by size: 3869 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3271 3731 5266 5453 1700 Size: 110 1461 460 1535 187 2869 Fragments arranged by size: 2869 1535 1461 460 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6622 BssHII G'CGCG_C Cuts at: 1925 3503 3597 3669 1925 Size: 1578 94 72 4878 Fragments arranged by size: 4878 1578 94 72 BssSI C'ACGA_G Cuts at: 3350 4682 5694 3350 Size: 1332 1012 4278 Fragments arranged by size: 4278 1332 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6622 BstBI TT'CG_AA Cuts at: 5426 5426 Size: 6622 BstEII G'GTnAC_C Cuts at: 1184 2894 1184 Size: 1710 4912 BstYI r'GATC_y Cuts at: 1972 2389 2619 2901 3952 4319 4704 4721 6173 Size: 417 230 282 1051 367 385 17 1452 Cuts at: 6173 6259 6271 6617 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1051 417 385 367 346 282 230 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 3160 3174 3261 3282 3481 Size: 552 939 48 1108 14 87 21 199 Cuts at: 3481 3536 3634 3805 3820 513 Size: 55 98 171 15 3315 Fragments arranged by size: 3315 1108 939 552 199 171 98 87 55 48 21 15 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5883 Fragments arranged by size: 5883 683 56 BtsI GCAGTG_nn' Cuts at: 2174 3154 4138 4932 4952 2174 Size: 980 984 794 20 3844 Fragments arranged by size: 3844 984 980 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2829 2864 529 Size: 35 2265 35 4287 Fragments arranged by size: 4287 2265 35 35 DraI TTT'AAA Cuts at: 3926 3926 Size: 6622 DraIII CAC_nnn'GTG Cuts at: 2858 2858 Size: 6622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5629 809 Size: 507 4313 1802 Fragments arranged by size: 4313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 3273 3316 Size: 54 1425 50 275 18 267 1176 43 Cuts at: 3316 3823 4069 4958 6587 8 Size: 507 246 889 1629 43 Fragments arranged by size: 1629 1425 1176 889 507 275 267 246 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3273 3823 4069 1537 Size: 275 1461 550 246 4090 Fragments arranged by size: 4090 1461 550 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2042 2111 2606 3854 4552 1353 Size: 689 69 495 1248 698 3423 Fragments arranged by size: 3423 1248 698 689 495 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3041 5209 5583 5729 387 Size: 1064 138 1452 2168 374 146 1280 Fragments arranged by size: 2168 1452 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2483 2889 3190 3478 3668 3815 Size: 393 419 432 406 301 288 190 147 Cuts at: 3815 4685 5282 6069 6337 1239 Size: 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 597 432 419 406 393 301 288 268 190 147 EcoNI CCTnn'n_nnAGG Cuts at: 2796 3032 2796 Size: 236 6386 EcoO109I rG'GnC_Cy Cuts at: 2266 2935 3165 2266 Size: 669 230 5723 Fragments arranged by size: 5723 669 230 EcoRI G'AATT_C Cuts at: 3931 4309 3931 Size: 378 6244 FspI TGC'GCA Cuts at: 5128 5128 Size: 6622 HaeII r_GCGC'y Cuts at: 1678 3382 3610 3630 3693 5769 1678 Size: 1704 228 20 63 2076 2531 Fragments arranged by size: 2531 2076 1704 228 63 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3110 3142 3227 3259 3641 Size: 32 135 32 1538 32 85 32 382 Cuts at: 3641 3673 3746 3778 5263 5295 5337 5369 1373 Size: 32 73 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1538 1485 382 135 85 73 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3966 4212 669 Size: 988 281 2028 246 3079 Fragments arranged by size: 3079 2028 988 281 246 HindIII A'AGCT_T Cuts at: 748 2405 748 Size: 1657 4965 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KasI G'GCGC_C Cuts at: 3626 3626 Size: 6622 KpnI G_GTAC'C Cuts at: 2743 3947 2743 Size: 1204 5418 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 3759 6498 3759 Size: 2739 3883 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3213 3789 3923 5736 5920 1305 Size: 1908 576 134 1813 184 2007 Fragments arranged by size: 2007 1908 1813 576 184 134 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 10 Size: 54 1425 343 267 4533 Fragments arranged by size: 4533 1425 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 2822 3266 4580 4939 5098 518 Size: 648 1656 444 1314 359 159 2042 Fragments arranged by size: 2042 1656 1314 648 444 359 159 MspA1I CmG'CkG Cuts at: 1606 2424 2649 2667 3162 3322 3402 3483 3538 Size: 818 225 18 495 160 80 81 55 Cuts at: 3538 3636 3807 3822 4038 4715 5863 6108 1606 Size: 98 171 15 216 677 1148 245 2120 Fragments arranged by size: 2120 1148 818 677 495 245 225 216 171 160 98 81 80 55 18 15 NaeI GCC'GGC Cuts at: 3273 3733 3273 Size: 460 6162 NarI GG'CG_CC Cuts at: 3627 3627 Size: 6622 NcoI C'CATG_G Cuts at: 513 1065 2004 2052 3174 3282 513 Size: 552 939 48 1122 108 3853 Fragments arranged by size: 3853 1122 939 552 108 48 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NgoMIV G'CCGG_C Cuts at: 3271 3731 3271 Size: 460 6162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 3823 4069 3823 Size: 246 6376 NspI r_CATG'y Cuts at: 1132 1229 2221 2821 3980 5525 1132 Size: 97 992 600 1159 1545 2229 Fragments arranged by size: 2229 1545 1159 992 600 97 PasI CC'CwG_GG Cuts at: 1348 2341 1348 Size: 993 5629 PciI A'CATG_T Cuts at: 5521 5521 Size: 6622 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6622 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2266 2935 2266 Size: 669 5953 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6622 PsiI TTA'TAA Cuts at: 4192 4192 Size: 6622 PspOMI G'GGCC_C Cuts at: 2232 3164 2232 Size: 932 5690 PstI C_TGCA'G Cuts at: 830 2161 3974 830 Size: 1331 1813 3478 Fragments arranged by size: 3478 1813 1331 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 PvuII CAG'CTG Cuts at: 2649 2649 Size: 6622 SacII CC_GC'GG Cuts at: 3163 3484 3539 3637 3808 3823 3163 Size: 321 55 98 171 15 5962 Fragments arranged by size: 5962 321 171 98 55 15 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SanDI GG'GwC_CC Cuts at: 2266 2266 Size: 6622 SapI GCTCTTCn'nnn_ Cuts at: 2111 2111 Size: 6622 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 3434 4870 6582 3434 Size: 1436 1712 3474 Fragments arranged by size: 3474 1712 1436 SfcI C'TryA_G Cuts at: 826 1045 2157 2353 3784 3970 5105 5786 5977 Size: 219 1112 196 1431 186 1135 681 191 Cuts at: 5977 826 Size: 1471 Fragments arranged by size: 1471 1431 1135 1112 681 219 196 191 186 SfoI GGC'GCC Cuts at: 3628 3628 Size: 6622 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 2231 3949 1872 Size: 359 1718 4545 Fragments arranged by size: 4545 1718 359 SmlI C'TyrA_G Cuts at: 820 1017 1943 2700 4726 5627 5889 820 Size: 197 926 757 2026 901 262 1553 Fragments arranged by size: 2026 1553 926 901 757 262 197 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 2821 3980 1132 Size: 1689 1159 3774 Fragments arranged by size: 3774 1689 1159 SspI AAT'ATT Cuts at: 5 52 4546 5 Size: 47 4494 2081 Fragments arranged by size: 4494 2081 47 StuI AGG'CCT Cuts at: 1517 2064 1517 Size: 547 6075 StyI C'CwwG_G Cuts at: 513 1065 2004 2052 2211 2847 3174 3282 3423 Size: 552 939 48 159 636 327 108 141 Cuts at: 3423 4061 513 Size: 638 3074 Fragments arranged by size: 3074 939 638 636 552 327 159 141 108 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2554 3485 3583 4649 4666 4819 5004 Size: 1255 974 931 98 1066 17 153 185 Cuts at: 5004 5449 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1066 974 931 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2940 3432 4868 Size: 274 80 33 51 1443 963 492 1436 Cuts at: 4868 6580 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1436 963 492 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6582 6614 6582 Size: 32 6590 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6622 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2542 3289 2542 Size: 747 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6622 XmaI C'CCGG_G Cuts at: 1870 2229 3947 1870 Size: 359 1718 4545 Fragments arranged by size: 4545 1718 359 XmnI GAAnn'nnTTC Cuts at: 2064 3254 4751 2064 Size: 1190 1497 3935 Fragments arranged by size: 3935 1497 1190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI