(Circular) (Six-base) MAPSORT of: pFN21AB8907.seq Check: 5259 from: 1 to: 5422 pFN21AB8907 5422 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2097 2210 3485 4869 5137 1632 Size: 465 113 1275 1384 268 1917 Fragments arranged by size: 1917 1384 1275 465 268 113 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 4321 5298 1203 Size: 3118 977 1327 Fragments arranged by size: 3118 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AlwNI CAG_nnn'CTG Cuts at: 2242 3165 4737 2242 Size: 923 1572 2927 Fragments arranged by size: 2927 1572 923 ApaI G_GGCC'C Cuts at: 2486 2486 Size: 5422 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2731 2957 3109 5218 1493 Size: 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2747 1870 Size: 73 804 4545 Fragments arranged by size: 4545 804 73 BamHI G'GATC_C Cuts at: 2752 3119 2752 Size: 367 5055 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2743 4198 618 Size: 325 217 627 339 617 1455 1842 Fragments arranged by size: 1842 1455 627 617 339 325 217 BanII G_rGCy'C Cuts at: 1391 2360 2486 2497 2741 1391 Size: 969 126 11 244 4072 Fragments arranged by size: 4072 969 244 126 11 BbeI G_GCGC'C Cuts at: 2130 2130 Size: 5422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2075 928 Size: 971 176 4275 Fragments arranged by size: 4275 971 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2349 4033 136 Size: 107 122 71 631 605 677 1684 1525 Fragments arranged by size: 1684 1525 677 631 605 122 107 71 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2839 2839 Size: 5422 Bme1580I G_kGCm'C Cuts at: 1790 2486 3483 4639 1790 Size: 696 997 1156 2573 Fragments arranged by size: 2573 1156 997 696 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2524 3153 4111 Size: 416 53 83 399 24 1107 629 958 Cuts at: 4111 442 Size: 1753 Fragments arranged by size: 1753 1107 958 629 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4082 1239 Size: 2843 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2353 2608 3547 4412 4710 2353 Size: 255 939 865 298 3065 Fragments arranged by size: 3065 939 865 298 255 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4085 882 Size: 617 2586 2219 Fragments arranged by size: 2586 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3213 493 Size: 711 2009 2702 Fragments arranged by size: 2702 2009 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 2127 2147 3229 Size: 53 83 186 936 497 97 20 1082 Cuts at: 3229 3611 4246 275 Size: 382 635 1451 Fragments arranged by size: 1451 1082 936 635 497 382 186 97 83 53 20 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5197 Fragments arranged by size: 5197 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 1186 Size: 1117 4305 BseYI C'CCAG_C Cuts at: 2113 2382 2462 2665 4625 5121 2113 Size: 269 80 203 1960 496 2414 Fragments arranged by size: 2414 1960 496 269 203 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4849 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2416 2872 3633 4661 1540 Size: 275 186 415 456 761 1028 2301 Fragments arranged by size: 2301 1028 761 456 415 275 186 BsiHKAI G_wGCw'C Cuts at: 3483 3568 4639 3483 Size: 85 1071 4266 Fragments arranged by size: 4266 1071 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2360 2486 2497 2741 3483 3568 4639 Size: 399 570 126 11 244 742 85 1071 Cuts at: 4639 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 570 399 244 126 85 11 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 2217 2777 844 Size: 1202 171 560 3489 Fragments arranged by size: 3489 1202 560 171 BsrBI CCG'CTC Cuts at: 1394 1823 2293 2367 3309 1394 Size: 429 470 74 942 3507 Fragments arranged by size: 3507 942 470 429 74 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 3917 4091 65 Size: 1183 1063 1606 174 1396 Fragments arranged by size: 1606 1396 1183 1063 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 2415 4066 4253 1700 Size: 110 346 259 1651 187 2869 Fragments arranged by size: 2869 1651 346 259 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 3482 4494 3482 Size: 1012 4410 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5422 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 2245 1184 Size: 1061 4361 BstXI CCAn_nnnn'nTGG Cuts at: 2470 2470 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2229 2752 3119 3504 3521 4973 5059 5071 Size: 257 523 367 385 17 1452 86 12 Cuts at: 5071 5417 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 523 385 367 346 257 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2089 513 Size: 552 939 12 73 3846 Fragments arranged by size: 3846 939 552 73 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4683 Fragments arranged by size: 4683 683 56 BtsI GCAGTG_nn' Cuts at: 2277 2702 2938 3732 3752 2277 Size: 425 236 794 20 3947 Fragments arranged by size: 3947 794 425 236 20 ClaI AT'CG_AT Cuts at: 1554 2220 1554 Size: 666 4756 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2020 4429 809 Size: 507 704 2409 1802 Fragments arranged by size: 2409 1802 704 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2158 2869 3758 Size: 54 1425 50 275 18 328 711 889 Cuts at: 3758 5387 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 711 328 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 1537 Size: 275 1057 4090 Fragments arranged by size: 4090 1057 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3352 1353 Size: 1999 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4009 4383 4529 387 Size: 1064 138 2420 374 146 1280 Fragments arranged by size: 2420 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2097 2210 3485 4082 4869 5137 1239 Size: 393 465 113 1275 597 787 268 1524 Fragments arranged by size: 1524 1275 787 597 465 393 268 113 EcoO109I rG'GnC_Cy Cuts at: 2482 2482 Size: 5422 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2686 2686 Size: 5422 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 2130 2189 4569 1678 Size: 452 59 2380 2531 Fragments arranged by size: 2531 2380 452 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 4063 4095 4137 Size: 32 135 32 842 32 1617 32 42 Cuts at: 4137 4169 1373 Size: 32 2626 Fragments arranged by size: 2626 1617 842 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2304 2766 3012 669 Size: 988 281 366 462 246 3079 Fragments arranged by size: 3079 988 462 366 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5422 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KasI G'GCGC_C Cuts at: 2126 2126 Size: 5422 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 4536 4720 1305 Size: 960 2271 184 2007 Fragments arranged by size: 2271 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3600 Fragments arranged by size: 3600 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 3380 3739 3898 518 Size: 648 1056 1158 359 159 2042 Fragments arranged by size: 2042 1158 1056 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2091 2242 2382 2838 3515 4663 4908 1606 Size: 485 151 140 456 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 485 456 245 151 140 NarI GG'CG_CC Cuts at: 2127 2127 Size: 5422 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2780 4325 1132 Size: 97 1551 1545 2229 Fragments arranged by size: 2229 1551 1545 97 PasI CC'CwG_GG Cuts at: 1348 2141 2609 1348 Size: 793 468 4161 Fragments arranged by size: 4161 793 468 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 2167 1108 Size: 1059 4363 PfoI T'CCnGG_A Cuts at: 2445 2445 Size: 5422 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PspOMI G'GGCC_C Cuts at: 2482 2482 Size: 5422 PstI C_TGCA'G Cuts at: 830 2238 2774 830 Size: 1408 536 3478 Fragments arranged by size: 3478 1408 536 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2242 2382 2242 Size: 140 5282 SacII CC_GC'GG Cuts at: 2092 2092 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 3670 5382 3670 Size: 1712 3710 SfcI C'TryA_G Cuts at: 826 1045 2234 2770 3905 4586 4777 826 Size: 219 1189 536 1135 681 191 1471 Fragments arranged by size: 1471 1189 1135 681 536 219 191 SfoI GGC'GCC Cuts at: 2128 2128 Size: 5422 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2368 2587 3526 4427 4689 820 Size: 197 926 425 219 939 901 262 1553 Fragments arranged by size: 1553 939 926 901 425 262 219 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2861 513 Size: 552 939 857 3074 Fragments arranged by size: 3074 939 857 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3449 3466 3619 3804 4249 325 Size: 1255 1869 17 153 185 445 1498 Fragments arranged by size: 1869 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3668 5380 96 Size: 274 80 33 51 1443 1691 1712 138 Fragments arranged by size: 1712 1691 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5382 5414 5382 Size: 32 5390 Tth111I GACn'n_nGTC Cuts at: 1316 2426 1316 Size: 1110 4312 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2554 2416 Size: 138 5284 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI