Restriction map for
pFN21AB6886
(Circular) (Six-base) MAPSORT of: pFN21AB6886.seq Check: 5949 from: 1 to: 5278
pFN21AB6886 5278 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3088 278
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2621 1937
Size: 684 4594
Acc65I G'GTAC_C
Cuts at: 2599 2599
Size: 5278
AclI AA'CG_TT
Cuts at: 1563 3405 3778 1563
Size: 1842 373 3063
Fragments arranged by size:
3063 1842 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2254 2567 3341 4725 4993 1632
Size: 622 313 774 1384 268 1917
Fragments arranged by size:
1917 1384 774 622 313 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5081
AflIII A'CryG_T
Cuts at: 1203 4177 5154 1203
Size: 2974 977 1327
Fragments arranged by size:
2974 1327 977
AgeI A'CCGG_T
Cuts at: 4109 4109
Size: 5278
AhdI GACnn_n'nnGTC
Cuts at: 4007 4007
Size: 5278
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5278
AlwNI CAG_nnn'CTG
Cuts at: 2089 2297 3021 4593 2089
Size: 208 724 1572 2774
Fragments arranged by size:
2774 1572 724 208
ApaLI G'TGCA_C
Cuts at: 3335 4491 3335
Size: 1156 4122
ApoI r'AATT_y
Cuts at: 1493 2107 2587 2813 2965 5074 1493
Size: 614 480 226 152 2109 1697
Fragments arranged by size:
2109 1697 614 480 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3832 160
Size: 872 2800 1606
Fragments arranged by size:
2800 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
AvaI C'yCGr_G
Cuts at: 1870 1943 2603 1870
Size: 73 660 4545
Fragments arranged by size:
4545 660 73
BamHI G'GATC_C
Cuts at: 2608 2975 2608
Size: 367 4911
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2599 4054 618
Size: 325 217 627 239 573 1455 1842
Fragments arranged by size:
1842 1455 627 573 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2070 2409 2597 1391
Size: 679 339 188 4072
Fragments arranged by size:
4072 679 339 188
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 5278
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2157 928
Size: 971 258 4049
Fragments arranged by size:
4049 971 258
BbvCI CC'TCA_GC
Cuts at: 2290 2290
Size: 5278
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3469 3503 1903
Size: 34 1532 34 3678
Fragments arranged by size:
3678 1532 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3170 4380 3170
Size: 1210 4068
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3889 136
Size: 107 122 71 631 605 2217 1525
Fragments arranged by size:
2217 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2121 5273 2121
Size: 3152 2126
BlpI GC'TnA_GC
Cuts at: 2259 2695 2259
Size: 436 4842
Bme1580I G_kGCm'C
Cuts at: 1790 3339 4495 1790
Size: 1549 1156 2573
Fragments arranged by size:
2573 1549 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5278
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3009 3967 442
Size: 416 53 83 399 24 1592 958 1753
Fragments arranged by size:
1753 1592 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2364 3938 1239
Size: 1125 1574 2579
Fragments arranged by size:
2579 1574 1125
Bpu10I CC'TnA_GC
Cuts at: 2290 2290
Size: 5278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3403 4268 4566 3403
Size: 865 298 4115
Fragments arranged by size:
4115 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3941 882
Size: 617 2442 2219
Fragments arranged by size:
2442 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3069 493
Size: 711 1865 2702
Fragments arranged by size:
2702 1865 711
BsaBI GATnn'nnATC
Cuts at: 2193 5272 2193
Size: 3079 2199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 3085 3467 4102
Size: 53 83 186 936 494 1058 382 635
Cuts at: 4102 275
Size: 1451
Fragments arranged by size:
1451 1058 936 635 494 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2241 3711 4109 4383 4530 4941 2241
Size: 1470 398 274 147 411 2578
Fragments arranged by size:
2578 1470 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5053
Fragments arranged by size:
5053 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5278
BseYI C'CCAG_C
Cuts at: 2184 2408 4481 4977 2184
Size: 224 2073 496 2485
Fragments arranged by size:
2485 2073 496 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 1163
Size: 573 729 3976
Fragments arranged by size:
3976 729 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2728 3489 4517 1540
Size: 275 186 727 761 1028 2301
Fragments arranged by size:
2301 1028 761 727 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 3339 3424 4495 2070
Size: 1269 85 1071 2853
Fragments arranged by size:
2853 1269 1071 85
BsmI GAATG_Cn'
Cuts at: 2789 2882 2789
Size: 93 5185
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2409 2597 3339 3424 4495 1391
Size: 399 280 339 188 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 339 280 188 85
BspEI T'CCGG_A
Cuts at: 4941 4941
Size: 5278
BspHI T'CATG_A
Cuts at: 3167 4897 3167
Size: 1730 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2633 844
Size: 1789 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2263 3165 1394
Size: 429 440 902 3507
Fragments arranged by size:
3507 902 440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3773 3947 65
Size: 1183 2525 174 1396
Fragments arranged by size:
2525 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2529 3922 4109 1700
Size: 110 719 1393 187 2869
Fragments arranged by size:
2869 1393 719 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5278
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5278
BssSI C'ACGA_G
Cuts at: 2069 2565 3338 4350 2069
Size: 496 773 1012 2997
Fragments arranged by size:
2997 1012 773 496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2306 794
Size: 1512 3766
BstBI TT'CG_AA
Cuts at: 4082 4082
Size: 5278
BstEII G'GTnAC_C
Cuts at: 1184 2284 1184
Size: 1100 4178
BstYI r'GATC_y
Cuts at: 1972 2037 2121 2428 2438 2608 2975 3360 3377
Size: 65 84 307 10 170 367 385 17
Cuts at: 3377 4829 4915 4927 5273 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 307 170 86
84 65 17 12 10
Bsu36I CC'TnA_GG
Cuts at: 2424 2485 2424
Size: 61 5217
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3787
Fragments arranged by size:
3787 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2024 487
Size: 683 56 798 3741
Fragments arranged by size:
3741 798 683 56
BtsI GCAGTG_nn'
Cuts at: 2794 3588 3608 2794
Size: 794 20 4464
Fragments arranged by size:
4464 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5243
DraI TTT'AAA
Cuts at: 2582 2582
Size: 5278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4285 809
Size: 507 2969 1802
Fragments arranged by size:
2969 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2725 3614 5243
Size: 54 1425 50 275 18 895 889 1629
Cuts at: 5243 8
Size: 43
Fragments arranged by size:
1629 1425 895 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2725 1537
Size: 275 913 4090
Fragments arranged by size:
4090 913 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 3208 1353
Size: 920 935 3423
Fragments arranged by size:
3423 935 920
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2078 2108 3865 4239 4385 387
Size: 1064 138 489 30 1757 374 146 1280
Fragments arranged by size:
1757 1280 1064 489 374 146 138 30
EcoICRI GAG'CTC
Cuts at: 2068 2068
Size: 5278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 2364 2567 3341 3938 4725 4993
Size: 393 622 110 203 774 597 787 268
Cuts at: 4993 1239
Size: 1524
Fragments arranged by size:
1524 787 774 622 597 393 268 203
110
EcoO109I rG'GnC_Cy
Cuts at: 2419 2419
Size: 5278
EcoRI G'AATT_C
Cuts at: 2587 2965 2587
Size: 378 4900
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2391 2391
Size: 5278
FspI TGC'GCA
Cuts at: 3784 3784
Size: 5278
HaeII r_GCGC'y
Cuts at: 1678 2030 4425 1678
Size: 352 2395 2531
Fragments arranged by size:
2531 2395 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3919 3951 3993 4025 1373
Size: 32 135 32 2347 32 42 32 2626
Fragments arranged by size:
2626 2347 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2622 2868 669
Size: 988 281 684 246 3079
Fragments arranged by size:
3079 988 684 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5278
HpaI GTT'AAC
Cuts at: 2868 2868
Size: 5278
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 5278
KpnI G_GTAC'C
Cuts at: 2603 2603
Size: 5278
MfeI C'AATT_G
Cuts at: 2877 2877
Size: 5278
MluI A'CGCG_T
Cuts at: 5154 5154
Size: 5278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4392 4576 1305
Size: 3087 184 2007
Fragments arranged by size:
3087 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3456
Fragments arranged by size:
3456 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2159 3236 3595 3754 518
Size: 648 843 150 1077 359 159 2042
Fragments arranged by size:
2042 1077 843 648 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2297 2694 3371 4519 4764 1606
Size: 691 397 677 1148 245 2120
Fragments arranged by size:
2120 1148 691 677 397 245
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 5278
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3787
Fragments arranged by size:
3787 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5278
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5278
NotI GC'GGCC_GC
Cuts at: 2725 2725
Size: 5278
NspI r_CATG'y
Cuts at: 1132 1229 2636 4181 1132
Size: 97 1407 1545 2229
Fragments arranged by size:
2229 1545 1407 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5278
PciI A'CATG_T
Cuts at: 4177 4177
Size: 5278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2357 1108
Size: 1249 4029
PfoI T'CCnGG_A
Cuts at: 2040 2040
Size: 5278
PmeI GTTT'AAAC
Cuts at: 2582 2582
Size: 5278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 4860 4892 3347
Size: 32 1481 32 3733
Fragments arranged by size:
3733 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 2419
Size: 5278
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5278
PsiI TTA'TAA
Cuts at: 2848 2848
Size: 5278
PstI C_TGCA'G
Cuts at: 830 2302 2630 830
Size: 1472 328 3478
Fragments arranged by size:
3478 1472 328
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5278
PvuII CAG'CTG
Cuts at: 2297 2297
Size: 5278
SacI G_AGCT'C
Cuts at: 2070 2070
Size: 5278
SalI G'TCGA_C
Cuts at: 1936 2620 1936
Size: 684 4594
SapI GCTCTTCn'nnn_
Cuts at: 2273 2273
Size: 5278
SbfI CC_TGCA'GG
Cuts at: 2630 2630
Size: 5278
ScaI AGT'ACT
Cuts at: 3526 5238 3526
Size: 1712 3566
SfcI C'TryA_G
Cuts at: 826 1045 2298 2626 3761 4442 4633 826
Size: 219 1253 328 1135 681 191 1471
Fragments arranged by size:
1471 1253 1135 681 328 219 191
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 5278
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5278
SmaI CCC'GGG
Cuts at: 1872 2605 1872
Size: 733 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3382 4283 4545 820
Size: 197 926 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3069 493
Size: 2576 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5278
SphI G_CATG'C
Cuts at: 1132 2636 1132
Size: 1504 3774
SspI AAT'ATT
Cuts at: 5 52 2173 3202 5
Size: 47 2121 1029 2081
Fragments arranged by size:
2121 2081 1029 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5278
StyI C'CwwG_G
Cuts at: 513 1065 2004 2717 513
Size: 552 939 713 3074
Fragments arranged by size:
3074 939 713 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3305 3322 3475 3660 4105 325
Size: 1255 1725 17 153 185 445 1498
Fragments arranged by size:
1725 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3524 5236 96
Size: 274 80 33 51 1443 1547 1712 138
Fragments arranged by size:
1712 1547 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2353 2385 5238 5270 2353
Size: 32 2853 32 2361
Fragments arranged by size:
2853 2361 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5278
XbaI T'CTAG_A
Cuts at: 2614 2614
Size: 5278
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5278
XmaI C'CCGG_G
Cuts at: 1870 2603 1870
Size: 733 4545
XmnI GAAnn'nnTTC
Cuts at: 3407 3407
Size: 5278
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3086 276
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI