(Circular) (Six-base) MAPSORT of: pFN21AE0304.seq Check: 6898 from: 1 to: 6172 pFN21AE0304 6172 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2457 3515 1937 Size: 520 1058 4594 Fragments arranged by size: 4594 1058 520 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2021 3225 4235 5619 5887 1632 Size: 389 1204 1010 1384 268 1917 Fragments arranged by size: 1917 1384 1204 1010 389 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 2103 5071 6048 1203 Size: 900 2968 977 1327 Fragments arranged by size: 2968 1327 977 900 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 2146 3173 3915 5487 2146 Size: 1027 742 1572 2831 Fragments arranged by size: 2831 1572 1027 742 ApaLI G'TGCA_C Cuts at: 4229 5385 4229 Size: 1156 5016 ApoI r'AATT_y Cuts at: 1493 2402 2912 2938 3145 3331 3481 3707 3859 Size: 909 510 26 207 186 150 226 152 Cuts at: 3859 5968 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 909 510 226 207 186 152 150 26 AseI AT'TA_AT Cuts at: 160 1032 2838 3408 4726 160 Size: 872 1806 570 1318 1606 Fragments arranged by size: 1806 1606 1318 872 570 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 3497 1870 Size: 73 1554 4545 Fragments arranged by size: 4545 1554 73 AvrII C'CTAG_G Cuts at: 3230 3230 Size: 6172 BamHI G'GATC_C Cuts at: 3502 3869 3502 Size: 367 5805 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3426 3493 4948 618 Size: 325 217 627 1639 67 1455 1842 Fragments arranged by size: 1842 1639 1455 627 325 217 67 BanII G_rGCy'C Cuts at: 1391 3491 1391 Size: 2100 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4363 4397 1903 Size: 34 2426 34 3678 Fragments arranged by size: 3678 2426 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 5274 4064 Size: 1210 4962 BclI T'GATC_A Cuts at: 1548 2820 1548 Size: 1272 4900 BfrBI ATG'CAT Cuts at: 2419 2419 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4783 136 Size: 107 122 71 631 605 3111 1525 Fragments arranged by size: 3111 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2851 6167 2851 Size: 3316 2856 BlpI GC'TnA_GC Cuts at: 2601 3589 2601 Size: 988 5184 Bme1580I G_kGCm'C Cuts at: 1790 4233 5389 1790 Size: 2443 1156 2573 Fragments arranged by size: 2573 2443 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3368 3903 4861 Size: 416 53 83 399 24 1951 535 958 Cuts at: 4861 442 Size: 1753 Fragments arranged by size: 1951 1753 958 535 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 2674 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2460 3416 4832 1239 Size: 968 253 956 1416 2579 Fragments arranged by size: 2579 1416 968 956 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2318 2956 4835 882 Size: 617 819 638 1879 2219 Fragments arranged by size: 2219 1879 819 638 617 BsaAI yAC'GTr Cuts at: 493 1204 3963 493 Size: 711 2759 2702 Fragments arranged by size: 2759 2702 711 BsaBI GATnn'nnATC Cuts at: 2487 6166 2487 Size: 3679 2493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3979 4361 4996 275 Size: 53 83 186 936 2446 382 635 1451 Fragments arranged by size: 2446 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2131 4605 5003 5277 5424 5835 2131 Size: 2474 398 274 147 411 2468 Fragments arranged by size: 2474 2468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 1178 Size: 30 165 30 672 30 5245 Fragments arranged by size: 5245 672 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 1186 Size: 871 5301 BseYI C'CCAG_C Cuts at: 2231 2745 5375 5871 2231 Size: 514 2630 496 2532 Fragments arranged by size: 2630 2532 514 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5599 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3622 4383 5411 1540 Size: 275 186 1621 761 1028 2301 Fragments arranged by size: 2301 1621 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2607 3417 4233 4318 5389 2607 Size: 810 816 85 1071 3390 Fragments arranged by size: 3390 1071 816 810 85 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 BsmBI CGTCTCn'nnnn_ Cuts at: 2282 2282 Size: 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2607 3417 3491 4233 4318 5389 1391 Size: 399 817 810 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 817 810 742 399 85 74 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 2584 4061 5791 2584 Size: 1477 1730 2965 Fragments arranged by size: 2965 1730 1477 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3527 844 Size: 2683 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4059 1394 Size: 429 2236 3507 Fragments arranged by size: 3507 2236 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2518 4667 4841 65 Size: 1183 1270 2149 174 1396 Fragments arranged by size: 2149 1396 1270 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4816 5003 1700 Size: 110 3006 187 2869 Fragments arranged by size: 3006 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6172 BssSI C'ACGA_G Cuts at: 4232 5244 4232 Size: 1012 5160 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6172 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstYI r'GATC_y Cuts at: 1972 2314 2851 3047 3502 3869 4254 4271 5723 Size: 342 537 196 455 367 385 17 1452 Cuts at: 5723 5809 5821 6167 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 537 455 385 367 346 342 196 86 17 12 BstZ17I GTA'TAC Cuts at: 2458 2458 Size: 6172 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4681 Fragments arranged by size: 4681 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2405 487 Size: 683 56 1179 4254 Fragments arranged by size: 4254 1179 683 56 BtsI GCAGTG_nn' Cuts at: 2027 3298 3688 4482 4502 2027 Size: 1271 390 794 20 3697 Fragments arranged by size: 3697 1271 794 390 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 2859 3476 2859 Size: 617 5555 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5179 809 Size: 507 3863 1802 Fragments arranged by size: 3863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2706 3619 4508 Size: 54 1425 50 275 18 876 913 889 Cuts at: 4508 6137 8 Size: 1629 43 Fragments arranged by size: 1629 1425 913 889 876 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3619 1537 Size: 275 1807 4090 Fragments arranged by size: 4090 1807 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3103 4102 1353 Size: 1750 999 3423 Fragments arranged by size: 3423 1750 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2078 4759 5133 5279 387 Size: 1064 138 489 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 489 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 2207 2460 3225 3416 4235 4832 Size: 393 389 186 253 765 191 819 597 Cuts at: 4832 5619 5887 1239 Size: 787 268 1524 Fragments arranged by size: 1524 819 787 765 597 393 389 268 253 191 186 EcoRI G'AATT_C Cuts at: 3331 3481 3859 3331 Size: 150 378 5644 Fragments arranged by size: 5644 378 150 EcoRV GAT'ATC Cuts at: 3135 3135 Size: 6172 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3165 3485 3165 Size: 320 5852 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2509 5319 1678 Size: 831 2810 2531 Fragments arranged by size: 2810 2531 831 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 4813 4845 4887 Size: 32 135 32 503 32 2706 32 42 Cuts at: 4887 4919 1373 Size: 32 2626 Fragments arranged by size: 2706 2626 503 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3516 3762 669 Size: 988 281 1578 246 3079 Fragments arranged by size: 3079 1578 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 2103 6048 2103 Size: 3945 2227 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5286 5470 1305 Size: 3981 184 2007 Fragments arranged by size: 3981 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4350 Fragments arranged by size: 4350 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2979 4130 4489 4648 518 Size: 648 1813 1151 359 159 2042 Fragments arranged by size: 2042 1813 1151 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 3588 4265 5413 5658 1606 Size: 579 1403 677 1148 245 2120 Fragments arranged by size: 2120 1403 1148 677 579 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4681 Fragments arranged by size: 4681 939 552 NdeI CA'TA_TG Cuts at: 387 2415 387 Size: 2028 4144 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NsiI A_TGCA'T Cuts at: 2421 2421 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 2351 2834 3530 5075 1132 Size: 97 1122 483 696 1545 2229 Fragments arranged by size: 2229 1545 1122 696 483 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6172 PciI A'CATG_T Cuts at: 5071 5071 Size: 6172 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6172 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PstI C_TGCA'G Cuts at: 830 3524 830 Size: 2694 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 2185 2185 Size: 6172 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SbfI CC_TGCA'GG Cuts at: 3524 3524 Size: 6172 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SfcI C'TryA_G Cuts at: 826 1045 3520 4655 5336 5527 826 Size: 219 2475 1135 681 191 1471 Fragments arranged by size: 2475 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6172 SmaI CCC'GGG Cuts at: 1872 3499 1872 Size: 1627 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4276 5177 5439 820 Size: 197 926 2333 901 262 1553 Fragments arranged by size: 2333 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6172 StyI C'CwwG_G Cuts at: 513 1065 2004 3053 3230 3611 513 Size: 552 939 1049 177 381 3074 Fragments arranged by size: 3074 1049 939 552 381 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2397 4199 4216 4369 4554 4999 325 Size: 1255 817 1802 17 153 185 445 1498 Fragments arranged by size: 1802 1498 1255 817 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3314 4418 6130 Size: 274 80 33 51 1443 1337 1104 1712 Cuts at: 6130 96 Size: 138 Fragments arranged by size: 1712 1443 1337 1104 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2093 2125 6132 6164 2093 Size: 32 4007 32 2101 Fragments arranged by size: 4007 2101 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2615 3194 2615 Size: 579 5593 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 3497 1870 Size: 1627 4545 XmnI GAAnn'nnTTC Cuts at: 2266 3118 3148 4301 2266 Size: 852 30 1153 4137 Fragments arranged by size: 4137 1153 852 30 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI