Restriction map for
pFN21AB6331
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BcgI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII CfoI ClaI CpoI
DpnII DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tsp45I
Tth111I Van91I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwNI 148 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 747 CCTTAGG CC^T[ACGT]AGG
AspHI 205 GTGCTC G[AT]GC[AT]^C
AvaI 449 CTCGAG C^[CT]CG[GA]G
AvaII 31 GGACC G^G[AT]CC
BanI 524 GGCACC G^G[CT][GA]CC
BciVI 191 GGATAC GTATCC(6/5)
BcnI 686 CCCGG CC^[GC]GG
BmyI 205 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 205 GTGCTC G[AT]GC[AT]^C
BsoBI 449 CTCGAG C^[CT]CG[GA]G
Bsp1286I 205 GTGCTC G[AGT]GC[ACT]^C
BsrDI 105 CATTGC GCAATG(2/0)
BsrFI 275 ACCGGC [GA]^CCGG[CT]
BsrI 162 CCAGT ACTGG(1/-1)
BssSI 83 CTCGTG CACGAG(-5/-1)
Bsu36I 747 CCTTAGG CC^T[ACGT]AGG
Cfr10I 275 ACCGGC [GA]^CCGG[CT]
DraII 149 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 747 CCTTAGG CC^T[ACGT]AGG
EcoNI 387 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 149 AGGCCCT [GA]G^G[ACGT]CC[CT]
HinfI 484 GACTC G^A[ACGT]TC
MslI 504 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 686 CCCGG CC^[GC]GG
NlaIV 524 GGCACC GG[ACGT]^[ACGT]CC
NspI 569 GCATGT [GA]CATG^[CT]
PaeR7I 449 CTCGAG C^TCGAG
PleI 484 GACTC GAGTC(4/5)
PstI 219 CTGCAG CTGCA^G
TaqI 450 TCGA T^CGA
Tsp509I 756 AATT ^AATT
TthHB8I 450 TCGA T^CGA
XcmI 470 CCAGGCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 449 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BbvCI 18 CCTCAGC CCTCAGC(-5/-2)
252 GCTGAGG
BpmI 331 CTGGAG CTGGAG(16/14)
666 CTGGAG
BseRI 16 CTCCTC GAGGAG(10/8)
705 CTCCTC
BsmAI 695 GAGAC GTCTC(1/5)
779 GTCTC
BsmFI 230 GGGAC GGGAC(10/14)
377 GGGAC
EarI 42 CTCTTC CTCTTC(1/4)
423 CTCTTC
HphI 763 GGTGA GGTGA(8/7)
211 TCACC
Ksp632I 42 CTCTTC CTCTTC(1/4)
423 CTCTTC
MboII 43 TCTTC GAAGA(8/7)
424 TCTTC
SfcI 219 CTGCAG C^T[GA][CT]AG
294 CTACAG
SmlI 449 CTCGAG C^T[CT][GA]AG
592 CTCAAG
StuI 335 AGGCCT AGG^CCT
722 AGGCCT
TspRI 126 CACTG CA[GC]TG[ACGT][ACGT]^
203 CAGTG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsgI BsiYI BslI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MnlI MspA1I MspI MvaI
MwoI NlaIII PvuII RsaI Sau96I
ScrFI SfaNI TseI