Restriction map for
pFN21AB6331
(Circular) (Six-base) MAPSORT of: pFN21AB6331.seq Check: 3903 from: 1 to: 5488
pFN21AB6331 5488 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2605 2617 3551 4935 5203 1632
Size: 973 12 934 1384 268 1917
Fragments arranged by size:
1917 1384 973 934 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 2158 3231 4803 2158
Size: 1073 1572 2843
Fragments arranged by size:
2843 1572 1073
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2454 2813 1870
Size: 73 511 359 4545
Fragments arranged by size:
4545 511 359 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2109 2142 2153 2186 2109
Size: 33 11 33 5411
Fragments arranged by size:
5411 33 33 11
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2529 2809 4264 618
Size: 325 217 627 742 280 1455 1842
Fragments arranged by size:
1842 1455 742 627 325 280 217
BanII G_rGCy'C
Cuts at: 1391 2807 1391
Size: 1416 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BbvCI CC'TCA_GC
Cuts at: 2024 2258 2024
Size: 234 5254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2190 3380 4590 2190
Size: 1190 1210 3088
Fragments arranged by size:
3088 1210 1190
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4099 136
Size: 107 122 71 631 605 2427 1525
Fragments arranged by size:
2427 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 3549 4705 1790
Size: 1759 1156 2573
Fragments arranged by size:
2573 1759 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2161 3219 4177
Size: 416 53 83 399 24 744 1058 958
Cuts at: 4177 442
Size: 1753
Fragments arranged by size:
1753 1058 958 744 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2723 2032
Size: 691 4797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2357 2692 4148 1239
Size: 1118 335 1456 2579
Fragments arranged by size:
2579 1456 1118 335
Bpu10I CC'TnA_GC
Cuts at: 2024 2151 2258 2024
Size: 127 107 5254
Fragments arranged by size:
5254 127 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3613 4478 4776 2582
Size: 1031 865 298 3294
Fragments arranged by size:
3294 1031 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3279 493
Size: 711 2075 2702
Fragments arranged by size:
2702 2075 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 3677 4312 275
Size: 53 83 186 936 1762 382 635 1451
Fragments arranged by size:
1762 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2693 2723 1178
Size: 30 165 30 1290 30 3943
Fragments arranged by size:
3943 1290 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2701 1186
Size: 826 689 3973
Fragments arranged by size:
3973 826 689
BseYI C'CCAG_C
Cuts at: 2146 2241 2533 2695 4691 5187 2146
Size: 95 292 162 1996 496 2447
Fragments arranged by size:
2447 1996 496 292 162 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2254 2337 2381 1163
Size: 573 518 83 44 4270
Fragments arranged by size:
4270 573 518 83 44
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 3549 3634 4705 2214
Size: 1335 85 1071 2997
Fragments arranged by size:
2997 1335 1071 85
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2807 3549 3634 4705 1391
Size: 399 424 593 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 593 424 399 85
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 844
Size: 1999 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2109 3983 4157 65
Size: 1183 861 1874 174 1396
Fragments arranged by size:
1874 1396 1183 861 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 4132 4319 1700
Size: 110 470 1852 187 2869
Fragments arranged by size:
2869 1852 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 2088 3548 4560 2088
Size: 1460 1012 3016
Fragments arranged by size:
3016 1460 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483
Size: 846 367 385 17 1452 86 12 346
Cuts at: 5483 1972
Size: 1977
Fragments arranged by size:
1977 1452 846 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2753 2753
Size: 5488
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3798 3818 3004
Size: 794 20 4674
Fragments arranged by size:
4674 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2935 3824 5453
Size: 54 1425 50 275 18 1105 889 1629
Cuts at: 5453 8
Size: 43
Fragments arranged by size:
1629 1425 1105 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2053 2434 3418 1353
Size: 700 381 984 3423
Fragments arranged by size:
3423 984 700 381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 2278 4075 4449 4595 387
Size: 1064 138 455 234 1797 374 146 1280
Fragments arranged by size:
1797 1280 1064 455 374 234 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2605 2617 2692 3551 4148 4935
Size: 393 725 248 12 75 859 597 787
Cuts at: 4935 5203 1239
Size: 268 1524
Fragments arranged by size:
1524 859 787 725 597 393 268 248
75 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 5488
EcoO109I rG'GnC_Cy
Cuts at: 2155 2155
Size: 5488
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 5488
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 4635 1678
Size: 2957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 2693 2725 4129
Size: 32 135 32 456 32 633 32 1404
Cuts at: 4129 4161 4203 4235 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1404 633 456 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 3446 3805 3964 518
Size: 648 1347 933 359 159 2042
Fragments arranged by size:
2042 1347 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2637 2721 2904 3581 4729 4974 1606
Size: 729 302 84 183 677 1148 245 2120
Fragments arranged by size:
2120 1148 729 677 302 245 183 84
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2578 2846 4391 1132
Size: 97 1349 268 1545 2229
Fragments arranged by size:
2229 1545 1349 268 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2228 2840 830
Size: 1398 612 3478
Fragments arranged by size:
3478 1398 612
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
PvuII CAG'CTG
Cuts at: 2335 2637 2721 2335
Size: 302 84 5102
Fragments arranged by size:
5102 302 84
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2224 2299 2836 3971 4652 4843 826
Size: 219 1179 75 537 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 681 537 219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2454 2597 3592 4493 4755 820
Size: 197 926 511 143 995 901 262 1553
Fragments arranged by size:
1553 995 926 901 511 262 197 143
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 2342 2729 1517
Size: 825 387 4276
Fragments arranged by size:
4276 825 387
StyI C'CwwG_G
Cuts at: 513 1065 2004 2927 513
Size: 552 939 923 3074
Fragments arranged by size:
3074 939 923 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2547 3515 3532 3685 3870 4315
Size: 1255 675 292 968 17 153 185 445
Cuts at: 4315 325
Size: 1498
Fragments arranged by size:
1498 1255 968 675 445 292 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2646 3734 5446
Size: 274 80 33 51 1443 669 1088 1712
Cuts at: 5446 96
Size: 138
Fragments arranged by size:
1712 1443 1088 669 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2482 2482
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 2454 1943
Size: 511 4977
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI