Restriction map for
pFN21AB6331
(Linear) (Six-base) MAPSORT of: KIBB6331.seq Check: 4383 from: 1 to: 783
KIBB6331.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 612 783
Size: 600 12 171
Fragments arranged by size:
600 171 12
AlwNI CAG_nnn'CTG
Cuts at: 0 153 783
Size: 153 630
AvaI C'yCGr_G
Cuts at: 0 449 783
Size: 449 334
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 104 137 148 181 783
Size: 104 33 11 33 602
Fragments arranged by size:
602 104 33 33 11
BanI G'GyrC_C
Cuts at: 0 524 783
Size: 524 259
BbvCI CC'TCA_GC
Cuts at: 0 19 253 783
Size: 19 234 530
Fragments arranged by size:
530 234 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 185 783
Size: 185 598
BmrI ACTGGGnnnn_n'
Cuts at: 0 156 783
Size: 156 627
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 718 783
Size: 27 691 65
Fragments arranged by size:
691 65 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 687 783
Size: 352 335 96
Fragments arranged by size:
352 335 96
Bpu10I CC'TnA_GC
Cuts at: 0 19 146 253 783
Size: 19 127 107 530
Fragments arranged by size:
530 127 107 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 783
Size: 577 206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 688 718 783
Size: 688 30 65
Fragments arranged by size:
688 65 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 696 783
Size: 7 689 87
Fragments arranged by size:
689 87 7
BseYI C'CCAG_C
Cuts at: 0 141 236 528 690 783
Size: 141 95 292 162 93
Fragments arranged by size:
292 162 141 95 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 332 376 783
Size: 249 83 44 407
Fragments arranged by size:
407 249 83 44
BsiHKAI G_wGCw'C
Cuts at: 0 209 783
Size: 209 574
Bsp1286I G_dGCh'C
Cuts at: 0 209 783
Size: 209 574
BsrDI GCAATG_nn'
Cuts at: 0 104 783
Size: 104 679
BsrFI r'CCGG_y
Cuts at: 0 275 783
Size: 275 508
BssSI C'ACGA_G
Cuts at: 0 83 783
Size: 83 700
Bsu36I CC'TnA_GG
Cuts at: 0 748 783
Size: 748 35
EarI CTCTTCn'nnn_
Cuts at: 0 48 429 783
Size: 48 381 354
Fragments arranged by size:
381 354 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 39 273 783
Size: 39 234 510
Fragments arranged by size:
510 234 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 600 612 687 783
Size: 352 248 12 75 96
Fragments arranged by size:
352 248 96 75 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 783
Size: 391 392
EcoO109I rG'GnC_Cy
Cuts at: 0 150 783
Size: 150 633
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 630 783
Size: 630 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 23 55 688 720 783
Size: 23 32 633 32 63
Fragments arranged by size:
633 63 32 32 23
MslI CAynn'nnrTG
Cuts at: 0 508 783
Size: 508 275
MspA1I CmG'CkG
Cuts at: 0 330 632 716 783
Size: 330 302 84 67
Fragments arranged by size:
330 302 84 67
NspI r_CATG'y
Cuts at: 0 573 783
Size: 573 210
PstI C_TGCA'G
Cuts at: 0 223 783
Size: 223 560
PvuII CAG'CTG
Cuts at: 0 330 632 716 783
Size: 330 302 84 67
Fragments arranged by size:
330 302 84 67
SfcI C'TryA_G
Cuts at: 0 219 294 783
Size: 219 75 489
Fragments arranged by size:
489 219 75
SmlI C'TyrA_G
Cuts at: 0 449 592 783
Size: 449 143 191
Fragments arranged by size:
449 191 143
StuI AGG'CCT
Cuts at: 0 337 724 783
Size: 337 387 59
Fragments arranged by size:
387 337 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 542 783
Size: 250 292 241
Fragments arranged by size:
292 250 241
TatI w'GTAC_w
Cuts at: 0 641 783
Size: 641 142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 477 783
Size: 477 306
XhoI C'TCGA_G
Cuts at: 0 449 783
Size: 449 334
Enzymes that do cut:
AcuI AlwNI AvaI BaeI BanI BbvCI BciVI BmrI
BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrDI BsrFI BssSI Bsu36I EarI EciI
Eco57MI EcoNI EcoO109I FalI Hin4I MslI MspA1I NspI
PstI PvuII SfcI SmlI StuI TaqII TatI XcmI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BanII BbeI BbsI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TstI Tth111I XbaI
XmaI XmnI ZraI