Restriction map for
pFN21AE0355
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I AspI AviII AvrII
BamHI BbeI BbrPI BclI BfrI
BlnI BsaBI BsaWI BseAI BsgI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BssHII BstAPI BstEII BstPI
ClaI CpoI DraI DraIII DrdI
EarI Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI Ksp632I MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RcaI RsrII SapI SbfI
ScaI SexAI SfoI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 1164 GACGTC GACGT^C
Acc65I 1802 GGTACC G^GTACC
AhdI 1550 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1393 GTGCAC G^TGCAC
ApaLI 1393 GTGCAC G^TGCAC
Asp718I 1802 GGTACC G^GTACC
AspEI 1550 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1886 TGGCCA TGG^CCA
BcgI 668 GCAGCCAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 897 GTATCC GTATCC(6/5)
BglII 386 AGATCT A^GATCT
BlpI 828 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 828 GCTCAGC GC^T[ACGT]AGC
BsaAI 1858 TACGTG [CT]AC^GT[GA]
BsmI 1443 GAATGC GAATGC(1/-1)
BsrDI 994 CATTGC GCAATG(2/0)
Bst1107I 340 GTATAC GTA^TAC
BstBI 1288 TTCGAA TT^CGAA
BstXI 68 CCATCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 340 GTATAC GTA^TAC
CelII 828 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1550 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 438 GACGC GACGC(5/10)
HincII 249 GTCGAC GT[CT]^[GA]AC
HindII 249 GTCGAC GT[CT]^[GA]AC
KpnI 1802 GGTACC GGTAC^C
MluNI 1886 TGGCCA TGG^CCA
MscI 1886 TGGCCA TGG^CCA
NheI 219 GCTAGC G^CTAGC
NspI 998 GCATGC [GA]CATG^[CT]
NspV 1288 TTCGAA TT^CGAA
PleI 412 GAGTC GAGTC(4/5)
PvuI 848 CGATCG CGAT^CG
SalI 249 GTCGAC G^TCGAC
SfuI 1288 TTCGAA TT^CGAA
SmlI 775 CTCAAG C^T[CT][GA]AG
SphI 998 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 438 GACGCC G[GA]^CG[CT]C
1164 GACGTC
AlwI 1242 GATCC GGATC(4/5)
1610 GGATC
BanI 1699 GGTGCC G^G[CT][GA]CC
1802 GGTACC
BbvCI 1753 CCTCAGC CCTCAGC(-5/-2)
1737 GCTGAGG
BsaHI 438 GACGCC G[GA]^CG[CT]C
1164 GACGTC
BsaI 816 GGTCTC GGTCTC(1/5)
1082 GGTCTC
Bsp1407I 884 TGTACA T^GTACA
1382 TGTACA
BspMI 824 ACCTGC ACCTGC(4/8)
1709 ACCTGC
BsrBI 1171 GAGCGG CCGCTC(-3/-3)
1743 GAGCGG
BsrGI 884 TGTACA T^GTACA
1382 TGTACA
BssSI 1795 CACGAG CACGAG(-5/-1)
802 CTCGTG
BstYI 386 AGATCT [GA]^GATC[CT]
1241 AGATCC
EcoT14I 1313 CCAAGG C^C[AT][AT]GG
1502 CCAAGG
Hin1I 438 GACGCC G[GA]^CG[CT]C
1164 GACGTC
HphI 27 GGTGA GGTGA(8/7)
1090 TCACC
KspI 1030 CCGCGG CCGC^GG
1229 CCGCGG
MaeII 1165 ACGT A^CGT
1859 ACGT
MflI 386 AGATCT [GA]^GATC[CT]
1241 AGATCC
MslI 224 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
331 CACCGCCGTG
NaeI 322 GCCGGC GCC^GGC
629 GCCGGC
NgoMIV 322 GCCGGC G^CCGGC
629 GCCGGC
PpuMI 791 GGGACCT [GA]G^G[AT]CC[CT]
1142 AGGACCT
SacI 1432 GAGCTC GAGCT^C
1543 GAGCTC
SacII 1030 CCGCGG CCGC^GG
1229 CCGCGG
SfiI 3 GGCCGCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1500 GGCCAAGGGGGCC
SspBI 884 TGTACA T^GTACA
1382 TGTACA
StyI 1313 CCAAGG C^C[AT][AT]GG
1502 CCAAGG
TfiI 465 GAATC G^A[AT]TC
783 GAATC
XhoII 386 AGATCT [GA]^GATC[CT]
1241 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII ItaI MaeI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI TaqI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI