(Circular) (Six-base) MAPSORT of: pFN21AE0355.seq Check: 8451 from: 1 to: 6598 pFN21AE0355 6598 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2838 2838 Size: 6598 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3173 4408 278 Size: 53 83 186 936 1637 1235 2468 Fragments arranged by size: 2468 1637 1235 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2255 2346 3261 3941 1937 Size: 318 91 915 680 4594 Fragments arranged by size: 4594 915 680 318 91 Acc65I G'GTAC_C Cuts at: 3807 3919 3807 Size: 112 6486 AclI AA'CG_TT Cuts at: 1563 4725 5098 1563 Size: 3162 373 3063 Fragments arranged by size: 3162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 2306 2390 2501 3248 4661 6045 6313 Size: 608 66 84 111 747 1413 1384 268 Cuts at: 6313 1632 Size: 1917 Fragments arranged by size: 1917 1413 1384 747 608 268 111 84 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 5497 6474 1203 Size: 4294 977 1327 Fragments arranged by size: 4294 1327 977 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 3560 5327 3560 Size: 1767 4831 AleI CACnn'nnGTG Cuts at: 1166 2233 2340 1166 Size: 1067 107 5424 Fragments arranged by size: 5424 1067 107 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6598 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3125 3157 3125 Size: 32 6566 AlwNI CAG_nnn'CTG Cuts at: 2239 2479 2866 3163 3226 4341 5913 2239 Size: 240 387 297 63 1115 1572 2924 Fragments arranged by size: 2924 1572 1115 387 297 240 63 ApaI G_GGCC'C Cuts at: 2251 2716 3678 2251 Size: 465 962 5171 Fragments arranged by size: 5171 962 465 ApaLI G'TGCA_C Cuts at: 3398 4655 5811 3398 Size: 1257 1156 4185 Fragments arranged by size: 4185 1257 1156 ApoI r'AATT_y Cuts at: 1493 3907 4133 4285 6394 1493 Size: 2414 226 152 2109 1697 Fragments arranged by size: 2414 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 2100 2387 2598 3017 3408 3879 3923 Size: 73 157 287 211 419 391 471 44 Cuts at: 3923 1870 Size: 4545 Fragments arranged by size: 4545 471 419 391 287 211 157 73 44 BamHI G'GATC_C Cuts at: 3928 4295 3928 Size: 367 6231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3704 3807 3919 5374 618 Size: 325 217 627 1917 103 112 1455 1842 Fragments arranged by size: 1917 1842 1455 627 325 217 112 103 BanII G_rGCy'C Cuts at: 1391 2251 2716 3024 3213 3409 3441 3552 3678 Size: 860 465 308 189 196 32 111 126 Cuts at: 3678 3917 1391 Size: 239 4072 Fragments arranged by size: 4072 860 465 308 239 196 189 126 111 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2543 3236 3642 928 Size: 971 644 693 406 3884 Fragments arranged by size: 3884 971 693 644 406 BbvCI CC'TCA_GC Cuts at: 3743 3759 3743 Size: 16 6582 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2662 2696 4789 4823 1903 Size: 34 725 34 2093 34 3678 Fragments arranged by size: 3678 2093 725 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2913 4490 5700 2913 Size: 1577 1210 3811 Fragments arranged by size: 3811 1577 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2069 2504 Size: 107 122 71 631 605 343 54 435 Cuts at: 2504 3413 3512 5209 136 Size: 909 99 1697 1525 Fragments arranged by size: 1697 1525 909 631 605 435 343 122 107 99 71 54 BglII A'GATC_T Cuts at: 2391 6593 2391 Size: 4202 2396 BlpI GC'TnA_GC Cuts at: 2834 4015 2834 Size: 1181 5417 Bme1580I G_kGCm'C Cuts at: 1790 2199 2251 2716 3402 3678 3856 4659 5815 Size: 409 52 465 686 276 178 803 1156 Cuts at: 5815 1790 Size: 2573 Fragments arranged by size: 2573 1156 803 686 465 409 276 178 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 4329 5287 Size: 416 53 83 399 24 828 2084 958 Cuts at: 5287 442 Size: 1753 Fragments arranged by size: 2084 1753 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2228 1056 Size: 1172 5426 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2384 2476 2384 Size: 92 6506 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2471 2597 2630 2753 3173 3587 3611 Size: 1022 210 126 33 123 420 414 24 Cuts at: 3611 3806 5258 1239 Size: 195 1452 2579 Fragments arranged by size: 2579 1452 1022 420 414 210 195 126 123 33 24 Bpu10I CC'TnA_GC Cuts at: 3422 3743 3759 3857 3422 Size: 321 16 98 6163 Fragments arranged by size: 6163 321 98 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2765 4723 5588 5886 2765 Size: 1958 865 298 3477 Fragments arranged by size: 3477 1958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2827 3093 5261 882 Size: 617 1328 266 2168 2219 Fragments arranged by size: 2219 2168 1328 617 266 BsaAI yAC'GTr Cuts at: 493 1204 3865 4389 493 Size: 711 2661 524 2702 Fragments arranged by size: 2702 2661 711 524 BsaBI GATnn'nnATC Cuts at: 6592 6592 Size: 6598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2444 3170 4405 4787 Size: 53 83 186 936 911 726 1235 382 Cuts at: 4787 5422 275 Size: 635 1451 Fragments arranged by size: 1451 1235 936 911 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5031 5429 5703 5850 6261 5031 Size: 398 274 147 411 5368 Fragments arranged by size: 5368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2126 2156 1178 Size: 30 165 30 723 30 5620 Fragments arranged by size: 5620 723 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2140 2468 2618 2642 3257 3476 3760 3803 Size: 954 328 150 24 615 219 284 43 Cuts at: 3803 3836 1186 Size: 33 3948 Fragments arranged by size: 3948 954 615 328 284 219 150 43 33 24 BseYI C'CCAG_C Cuts at: 2183 2319 2519 3023 3213 5801 6297 2183 Size: 136 200 504 190 2588 496 2484 Fragments arranged by size: 2588 2484 504 496 200 190 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6025 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2262 2332 2856 4048 4809 Size: 275 186 18 243 70 524 1192 761 Cuts at: 4809 5837 1540 Size: 1028 2301 Fragments arranged by size: 2301 1192 1028 761 524 275 243 186 70 18 BsiHKAI G_wGCw'C Cuts at: 2885 3402 3441 3552 4659 4744 5815 2885 Size: 517 39 111 1107 85 1071 3668 Fragments arranged by size: 3668 1107 1071 517 111 85 39 BsmI GAATG_Cn' Cuts at: 3454 4109 4202 3454 Size: 655 93 5850 Fragments arranged by size: 5850 655 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2251 2716 2885 3024 3213 3402 Size: 399 409 52 465 169 139 189 189 Cuts at: 3402 3409 3441 3552 3678 3856 3917 4659 4744 Size: 7 32 111 126 178 61 742 85 Cuts at: 4744 5815 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 465 409 399 189 189 178 169 139 126 111 85 61 52 32 7 BspEI T'CCGG_A Cuts at: 6261 6261 Size: 6598 BspHI T'CATG_A Cuts at: 4487 6217 4487 Size: 1730 4868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2838 3723 3953 844 Size: 1994 885 230 3489 Fragments arranged by size: 3489 1994 885 230 BsrBI CCG'CTC Cuts at: 1394 1823 3178 3750 4485 1394 Size: 429 1355 572 735 3507 Fragments arranged by size: 3507 1355 735 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2998 5093 5267 65 Size: 1183 1750 2095 174 1396 Fragments arranged by size: 2095 1750 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2087 2094 2327 2634 5242 5429 1700 Size: 110 277 7 233 307 2608 187 2869 Fragments arranged by size: 2869 2608 307 277 233 187 110 7 BsrGI T'GTAC_A Cuts at: 96 2889 3387 96 Size: 2793 498 3307 Fragments arranged by size: 3307 2793 498 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 2807 3800 4658 5670 2807 Size: 993 858 1012 3735 Fragments arranged by size: 3735 1012 993 858 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6598 BstBI TT'CG_AA Cuts at: 3294 5402 3294 Size: 2108 4490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6598 BstXI CCAn_nnnn'nTGG Cuts at: 2080 2080 Size: 6598 BstYI r'GATC_y Cuts at: 1972 2391 3246 3928 4295 4680 4697 6149 6235 Size: 419 855 682 367 385 17 1452 86 Cuts at: 6235 6247 6593 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 855 682 419 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2347 2347 Size: 6598 Bsu36I CC'TnA_GG Cuts at: 2123 2867 3501 2123 Size: 744 634 5220 Fragments arranged by size: 5220 744 634 BtgI C'CryG_G Cuts at: 513 1065 2004 3035 3081 3193 3234 513 Size: 552 939 1031 46 112 41 3877 Fragments arranged by size: 3877 1031 939 552 112 46 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3670 487 Size: 683 56 2444 3415 Fragments arranged by size: 3415 2444 683 56 BtsI GCAGTG_nn' Cuts at: 2240 2885 3842 4114 4908 4928 2240 Size: 645 957 272 794 20 3910 Fragments arranged by size: 3910 957 794 645 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3448 3483 529 Size: 35 2884 35 3644 Fragments arranged by size: 3644 2884 35 35 DraI TTT'AAA Cuts at: 3902 3902 Size: 6598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2016 2259 Size: 54 1425 50 275 18 177 9 243 Cuts at: 2259 2329 2756 3891 4045 4934 6563 8 Size: 70 427 1135 154 889 1629 43 Fragments arranged by size: 1629 1425 1135 889 427 275 243 177 154 70 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2016 2259 2329 4045 1537 Size: 275 204 243 70 1716 4090 Fragments arranged by size: 4090 1716 275 243 204 70 EarI CTCTTCn'nnn_ Cuts at: 1353 4528 1353 Size: 3175 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5185 5559 5705 387 Size: 1064 138 3596 374 146 1280 Fragments arranged by size: 3596 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3439 3550 3439 Size: 111 6487 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2240 2261 2306 2390 2471 2501 2597 Size: 393 608 21 45 84 81 30 96 Cuts at: 2597 2630 2753 3173 3248 3587 3611 3806 4661 Size: 33 123 420 75 339 24 195 855 Cuts at: 4661 5258 6045 6313 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 855 787 608 597 420 393 339 268 195 123 96 84 81 75 45 33 30 24 21 EcoNI CCTnn'n_nnAGG Cuts at: 3893 3893 Size: 6598 EcoO109I rG'GnC_Cy Cuts at: 2247 2713 2797 3148 3675 2247 Size: 466 84 351 527 5170 Fragments arranged by size: 5170 527 466 351 84 EcoRI G'AATT_C Cuts at: 3907 4285 3907 Size: 378 6220 FspI TGC'GCA Cuts at: 5104 5104 Size: 6598 HaeII r_GCGC'y Cuts at: 1678 2052 2635 2898 5745 1678 Size: 374 583 263 2847 2531 Fragments arranged by size: 2847 2531 583 374 263 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2539 2571 3098 3130 3546 Size: 32 135 32 967 32 527 32 416 Cuts at: 3546 3578 5239 5271 5313 5345 1373 Size: 32 1661 32 42 32 2626 Fragments arranged by size: 2626 1661 967 527 416 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2256 3942 4188 669 Size: 988 281 318 1686 246 3079 Fragments arranged by size: 3079 1686 988 318 281 246 HindIII A'AGCT_T Cuts at: 748 2057 2267 2402 748 Size: 1309 210 135 4944 Fragments arranged by size: 4944 1309 210 135 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KpnI G_GTAC'C Cuts at: 3811 3923 3811 Size: 112 6486 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3094 3750 5712 5896 1305 Size: 1789 656 1962 184 2007 Fragments arranged by size: 2007 1962 1789 656 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3893 10 Size: 54 1425 343 2061 2715 Fragments arranged by size: 2715 2061 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2233 2340 4556 4915 5074 518 Size: 648 1067 107 2216 359 159 2042 Fragments arranged by size: 2216 2042 1067 648 359 159 107 MspA1I CmG'CkG Cuts at: 1606 2012 2041 2306 2563 2710 3037 3217 3226 Size: 406 29 265 257 147 327 180 9 Cuts at: 3226 3236 3311 3327 3364 3695 3763 4014 4691 Size: 10 75 16 37 331 68 251 677 Cuts at: 4691 5839 6084 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 406 331 327 265 257 251 245 180 147 75 68 37 29 16 10 9 NaeI GCC'GGC Cuts at: 2329 2636 2329 Size: 307 6291 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5107 Fragments arranged by size: 5107 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NgoMIV G'CCGG_C Cuts at: 2327 2634 2327 Size: 307 6291 NheI G'CTAG_C Cuts at: 1052 2224 1052 Size: 1172 5426 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 3007 3956 5501 1132 Size: 97 1778 949 1545 2229 Fragments arranged by size: 2229 1778 1545 949 97 PasI CC'CwG_GG Cuts at: 1348 3100 1348 Size: 1752 4846 PciI A'CATG_T Cuts at: 5497 5497 Size: 6598 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6598 PfoI T'CCnGG_A Cuts at: 2646 2730 3564 2646 Size: 84 834 5680 Fragments arranged by size: 5680 834 84 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2797 3148 2797 Size: 351 6247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6598 PsiI TTA'TAA Cuts at: 4168 4168 Size: 6598 PspOMI G'GGCC_C Cuts at: 2247 2712 3674 2247 Size: 465 962 5171 Fragments arranged by size: 5171 962 465 PstI C_TGCA'G Cuts at: 830 2046 2305 2469 2880 3222 3694 3849 3950 Size: 1216 259 164 411 342 472 155 101 Cuts at: 3950 830 Size: 3478 Fragments arranged by size: 3478 1216 472 411 342 259 164 155 101 PvuI CG_AT'CG Cuts at: 2001 2856 2001 Size: 855 5743 PvuII CAG'CTG Cuts at: 2306 3217 3226 3311 3695 3763 2306 Size: 911 9 85 384 68 5141 Fragments arranged by size: 5141 911 384 85 68 9 SacI G_AGCT'C Cuts at: 3441 3552 3441 Size: 111 6487 SacII CC_GC'GG Cuts at: 3038 3237 3038 Size: 199 6399 SalI G'TCGA_C Cuts at: 1936 2254 3940 1936 Size: 318 1686 4594 Fragments arranged by size: 4594 1686 318 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 4846 6558 4846 Size: 1712 4886 SfcI C'TryA_G Cuts at: 826 1045 2042 2301 2465 2558 2876 3218 3442 Size: 219 997 259 164 93 318 342 224 Cuts at: 3442 3690 3845 3946 5081 5762 5953 826 Size: 248 155 101 1135 681 191 1471 Fragments arranged by size: 1471 1135 997 681 342 318 259 248 224 219 191 164 155 101 93 SfiI GGCCn_nnn'nGGCC Cuts at: 2015 3512 2015 Size: 1497 5101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 2102 2600 3410 3881 3925 1872 Size: 230 498 810 471 44 4545 Fragments arranged by size: 4545 810 498 471 230 44 SmlI C'TyrA_G Cuts at: 820 1017 1943 2780 4702 5603 5865 820 Size: 197 926 837 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 837 262 197 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6598 SphI G_CATG'C Cuts at: 1132 3007 3956 1132 Size: 1875 949 3774 Fragments arranged by size: 3774 1875 949 SspI AAT'ATT Cuts at: 5 52 4522 5 Size: 47 4470 2081 Fragments arranged by size: 4470 2081 47 StuI AGG'CCT Cuts at: 1517 2459 3300 3888 1517 Size: 942 841 588 4227 Fragments arranged by size: 4227 942 841 588 StyI C'CwwG_G Cuts at: 513 1065 2004 3318 3507 3894 4037 513 Size: 552 939 1314 189 387 143 3074 Fragments arranged by size: 3074 1314 939 552 387 189 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4625 4642 4795 4980 5425 325 Size: 1255 3045 17 153 185 445 1498 Fragments arranged by size: 3045 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2889 3387 4844 Size: 274 80 33 51 1443 912 498 1457 Cuts at: 4844 6556 96 Size: 1712 138 Fragments arranged by size: 1712 1457 1443 912 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 3607 3639 6558 6590 2126 Size: 32 1449 32 2919 32 2134 Fragments arranged by size: 2919 2134 1449 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6598 XbaI T'CTAG_A Cuts at: 3934 3934 Size: 6598 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6598 XmaI C'CCGG_G Cuts at: 1870 2100 2598 3408 3879 3923 1870 Size: 230 498 810 471 44 4545 Fragments arranged by size: 4545 810 498 471 230 44 XmnI GAAnn'nnTTC Cuts at: 4727 4727 Size: 6598 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3171 4406 276 Size: 53 83 186 936 1637 1235 2468 Fragments arranged by size: 2468 1637 1235 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfoI SrfI SwaI XcmI