Restriction map for
pFN21AE0355
(Linear) (Six-base) MAPSORT of: KIEE0355.seq Check: 158 from: 1 to: 1893
KIEE0355.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 833 1893
Size: 833 1060
AatII G_ACGT'C
Cuts at: 0 1168 1893
Size: 1168 725
AccI GT'mk_AC
Cuts at: 0 250 341 1256 1893
Size: 250 91 915 637
Fragments arranged by size:
915 637 250 91
Acc65I G'GTAC_C
Cuts at: 0 1802 1893
Size: 1802 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 301 385 496 1243 1893
Size: 235 66 84 111 747 650
Fragments arranged by size:
747 650 235 111 84 66
AhdI GACnn_n'nnGTC
Cuts at: 0 1555 1893
Size: 1555 338
AleI CACnn'nnGTG
Cuts at: 0 228 335 1893
Size: 228 107 1558
Fragments arranged by size:
1558 228 107
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1893
Size: 1120 32 741
Fragments arranged by size:
1120 741 32
AlwNI CAG_nnn'CTG
Cuts at: 0 234 474 861 1158 1221 1893
Size: 234 240 387 297 63 672
Fragments arranged by size:
672 387 297 240 234 63
ApaI G_GGCC'C
Cuts at: 0 246 711 1673 1893
Size: 246 465 962 220
Fragments arranged by size:
962 465 246 220
ApaLI G'TGCA_C
Cuts at: 0 1393 1893
Size: 1393 500
AvaI C'yCGr_G
Cuts at: 0 95 382 593 1012 1403 1874 1893
Size: 95 287 211 419 391 471 19
Fragments arranged by size:
471 419 391 287 211 95 19
BanI G'GyrC_C
Cuts at: 0 1699 1802 1893
Size: 1699 103 91
Fragments arranged by size:
1699 103 91
BanII G_rGCy'C
Cuts at: 0 246 711 1019 1208 1404 1436 1547 1673
Size: 246 465 308 189 196 32 111 126
Cuts at: 1673 1893
Size: 220
Fragments arranged by size:
465 308 246 220 196 189 126 111
32
BbsI GAAGACnn'nnnn_
Cuts at: 0 538 1231 1637 1893
Size: 538 693 406 256
Fragments arranged by size:
693 538 406 256
BbvCI CC'TCA_GC
Cuts at: 0 1738 1754 1893
Size: 1738 16 139
Fragments arranged by size:
1738 139 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 657 691 1893
Size: 657 34 1202
Fragments arranged by size:
1202 657 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 908 1893
Size: 908 985
BglI GCCn_nnn'nGGC
Cuts at: 0 10 64 499 1408 1507 1893
Size: 10 54 435 909 99 386
Fragments arranged by size:
909 435 386 99 54 10
BglII A'GATC_T
Cuts at: 0 386 1893
Size: 386 1507
BlpI GC'TnA_GC
Cuts at: 0 829 1893
Size: 829 1064
Bme1580I G_kGCm'C
Cuts at: 0 194 246 711 1397 1673 1851 1893
Size: 194 52 465 686 276 178 42
Fragments arranged by size:
686 465 276 194 178 52 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 1893
Size: 240 1653
BmtI G_CTAG'C
Cuts at: 0 223 1893
Size: 223 1670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 379 471 1893
Size: 379 92 1422
Fragments arranged by size:
1422 379 92
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 466 592 625 748 1168 1582 1606
Size: 256 210 126 33 123 420 414 24
Cuts at: 1606 1801 1893
Size: 195 92
Fragments arranged by size:
420 414 256 210 195 126 123 92
33 24
Bpu10I CC'TnA_GC
Cuts at: 0 1417 1738 1754 1852 1893
Size: 1417 321 16 98 41
Fragments arranged by size:
1417 321 98 41 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1893
Size: 760 1133
BsaI GGTCTCn'nnnn_
Cuts at: 0 822 1088 1893
Size: 822 266 805
Fragments arranged by size:
822 805 266
BsaAI yAC'GTr
Cuts at: 0 1860 1893
Size: 1860 33
BsaHI Gr'CG_yC
Cuts at: 0 439 1165 1893
Size: 439 726 728
Fragments arranged by size:
728 726 439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 121 151 1893
Size: 121 30 1742
Fragments arranged by size:
1742 121 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 135 463 613 637 1252 1471 1755 1798
Size: 135 328 150 24 615 219 284 43
Cuts at: 1798 1831 1893
Size: 33 62
Fragments arranged by size:
615 328 284 219 150 135 62 43
33 24
BseYI C'CCAG_C
Cuts at: 0 178 314 514 1018 1208 1893
Size: 178 136 200 504 190 685
Fragments arranged by size:
685 504 200 190 178 136
BsiEI CG_ry'CG
Cuts at: 0 14 257 327 851 1893
Size: 14 243 70 524 1042
Fragments arranged by size:
1042 524 243 70 14
BsiHKAI G_wGCw'C
Cuts at: 0 880 1397 1436 1547 1893
Size: 880 517 39 111 346
Fragments arranged by size:
880 517 346 111 39
BsmI GAATG_Cn'
Cuts at: 0 1449 1893
Size: 1449 444
Bsp1286I G_dGCh'C
Cuts at: 0 194 246 711 880 1019 1208 1397 1404
Size: 194 52 465 169 139 189 189 7
Cuts at: 1404 1436 1547 1673 1851 1893
Size: 32 111 126 178 42
Fragments arranged by size:
465 194 189 189 178 169 139 126
111 52 42 32 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 833 1718 1893
Size: 833 885 175
Fragments arranged by size:
885 833 175
BsrBI CCG'CTC
Cuts at: 0 1173 1745 1893
Size: 1173 572 148
Fragments arranged by size:
1173 572 148
BsrDI GCAATG_nn'
Cuts at: 0 993 1893
Size: 993 900
BsrFI r'CCGG_y
Cuts at: 0 82 89 322 629 1893
Size: 82 7 233 307 1264
Fragments arranged by size:
1264 307 233 82 7
BsrGI T'GTAC_A
Cuts at: 0 884 1382 1893
Size: 884 498 511
Fragments arranged by size:
884 511 498
BssSI C'ACGA_G
Cuts at: 0 802 1795 1893
Size: 802 993 98
Fragments arranged by size:
993 802 98
BstBI TT'CG_AA
Cuts at: 0 1289 1893
Size: 1289 604
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 1893
Size: 75 1818
BstYI r'GATC_y
Cuts at: 0 386 1241 1893
Size: 386 855 652
Fragments arranged by size:
855 652 386
BstZ17I GTA'TAC
Cuts at: 0 342 1893
Size: 342 1551
Bsu36I CC'TnA_GG
Cuts at: 0 118 862 1496 1893
Size: 118 744 634 397
Fragments arranged by size:
744 634 397 118
BtgI C'CryG_G
Cuts at: 0 1030 1076 1188 1229 1893
Size: 1030 46 112 41 664
Fragments arranged by size:
1030 664 112 46 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1665 1893
Size: 1665 228
BtsI GCAGTG_nn'
Cuts at: 0 235 880 1837 1893
Size: 235 645 957 56
Fragments arranged by size:
957 645 235 56
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1443 1478 1893
Size: 1443 35 415
Fragments arranged by size:
1443 415 35
EaeI y'GGCC_r
Cuts at: 0 2 11 254 324 751 1886 1893
Size: 2 9 243 70 427 1135 7
Fragments arranged by size:
1135 427 243 70 9 7 2
EagI C'GGCC_G
Cuts at: 0 11 254 324 1893
Size: 11 243 70 1569
Fragments arranged by size:
1569 243 70 11
EcoICRI GAG'CTC
Cuts at: 0 1434 1545 1893
Size: 1434 111 348
Fragments arranged by size:
1434 348 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 256 301 385 466 496 592 625
Size: 235 21 45 84 81 30 96 33
Cuts at: 625 748 1168 1243 1582 1606 1801 1893
Size: 123 420 75 339 24 195 92
Fragments arranged by size:
420 339 235 195 123 96 92 84
81 75 45 33 30 24 21
EcoO109I rG'GnC_Cy
Cuts at: 0 242 708 792 1143 1670 1893
Size: 242 466 84 351 527 223
Fragments arranged by size:
527 466 351 242 223 84
HaeII r_GCGC'y
Cuts at: 0 47 630 893 1893
Size: 47 583 263 1000
Fragments arranged by size:
1000 583 263 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 534 566 1093 1125 1541 1573 1893
Size: 534 32 527 32 416 32 320
Fragments arranged by size:
534 527 416 320 32 32 32
HincII GTy'rAC
Cuts at: 0 251 1893
Size: 251 1642
HindIII A'AGCT_T
Cuts at: 0 52 262 397 1893
Size: 52 210 135 1496
Fragments arranged by size:
1496 210 135 52
KpnI G_GTAC'C
Cuts at: 0 1806 1893
Size: 1806 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 1745 1893
Size: 1089 656 148
Fragments arranged by size:
1089 656 148
MscI TGG'CCA
Cuts at: 0 1888 1893
Size: 1888 5
MslI CAynn'nnrTG
Cuts at: 0 228 335 1893
Size: 228 107 1558
Fragments arranged by size:
1558 228 107
MspA1I CmG'CkG
Cuts at: 0 7 36 301 558 705 1032 1212 1221
Size: 7 29 265 257 147 327 180 9
Cuts at: 1221 1231 1306 1322 1359 1690 1758 1893
Size: 10 75 16 37 331 68 135
Fragments arranged by size:
331 327 265 257 180 147 135 75
68 37 29 16 10 9 7
NaeI GCC'GGC
Cuts at: 0 324 631 1893
Size: 324 307 1262
Fragments arranged by size:
1262 324 307
NgoMIV G'CCGG_C
Cuts at: 0 322 629 1893
Size: 322 307 1264
Fragments arranged by size:
1264 322 307
NheI G'CTAG_C
Cuts at: 0 219 1893
Size: 219 1674
NspI r_CATG'y
Cuts at: 0 1002 1893
Size: 1002 891
PasI CC'CwG_GG
Cuts at: 0 1095 1893
Size: 1095 798
PfoI T'CCnGG_A
Cuts at: 0 641 725 1559 1893
Size: 641 84 834 334
Fragments arranged by size:
834 641 334 84
PpuMI rG'GwC_Cy
Cuts at: 0 792 1143 1893
Size: 792 351 750
Fragments arranged by size:
792 750 351
PspOMI G'GGCC_C
Cuts at: 0 242 707 1669 1893
Size: 242 465 962 224
Fragments arranged by size:
962 465 242 224
PstI C_TGCA'G
Cuts at: 0 41 300 464 875 1217 1689 1844 1893
Size: 41 259 164 411 342 472 155 49
Fragments arranged by size:
472 411 342 259 164 155 49 41
PvuI CG_AT'CG
Cuts at: 0 851 1893
Size: 851 1042
PvuII CAG'CTG
Cuts at: 0 301 1212 1221 1306 1690 1758 1893
Size: 301 911 9 85 384 68 135
Fragments arranged by size:
911 384 301 135 85 68 9
SacI G_AGCT'C
Cuts at: 0 1436 1547 1893
Size: 1436 111 346
Fragments arranged by size:
1436 346 111
SacII CC_GC'GG
Cuts at: 0 1033 1232 1893
Size: 1033 199 661
Fragments arranged by size:
1033 661 199
SalI G'TCGA_C
Cuts at: 0 249 1893
Size: 249 1644
SfcI C'TryA_G
Cuts at: 0 37 296 460 553 871 1213 1437 1685
Size: 37 259 164 93 318 342 224 248
Cuts at: 1685 1840 1893
Size: 155 53
Fragments arranged by size:
342 318 259 248 224 164 155 93
53 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 10 1507 1893
Size: 10 1497 386
Fragments arranged by size:
1497 386 10
SmaI CCC'GGG
Cuts at: 0 97 595 1405 1876 1893
Size: 97 498 810 471 17
Fragments arranged by size:
810 498 471 97 17
SmlI C'TyrA_G
Cuts at: 0 775 1893
Size: 775 1118
SphI G_CATG'C
Cuts at: 0 1002 1893
Size: 1002 891
StuI AGG'CCT
Cuts at: 0 454 1295 1883 1893
Size: 454 841 588 10
Fragments arranged by size:
841 588 454 10
StyI C'CwwG_G
Cuts at: 0 1313 1502 1893
Size: 1313 189 391
Fragments arranged by size:
1313 391 189
TatI w'GTAC_w
Cuts at: 0 884 1382 1893
Size: 884 498 511
Fragments arranged by size:
884 511 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 1602 1634 1893
Size: 121 32 1449 32 259
Fragments arranged by size:
1449 259 121 32 32
XmaI C'CCGG_G
Cuts at: 0 95 593 1403 1874 1893
Size: 95 498 810 471 19
Fragments arranged by size:
810 498 471 95 19
ZraI GAC'GTC
Cuts at: 0 1166 1893
Size: 1166 727
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AhdI AleI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EcoICRI
Eco57MI EcoO109I HaeII Hin4I HincII HindIII KpnI MmeI
MscI MslI MspA1I NaeI NgoMIV NheI NspI PasI
PfoI PpuMI PspOMI PstI PvuI PvuII SacI SacII
SalI SfcI SfiI SmaI SmlI SphI StuI StyI
TatI TstI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AlfI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BclI BfrBI
BmgBI BsaBI BsaWI BsgI BsiWI BsmBI BspEI BspHI
BssHII BstAPI BstEII ClaI DraI DraIII DrdI EarI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HpaI KasI MfeI MluI NarI NcoI NdeI NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI RsrII SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI