(Circular) (Six-base) MAPSORT of: pFN21AE0111.seq Check: 8119 from: 1 to: 5743 pFN21AE0111 5743 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2332 3806 5190 5458 1632 Size: 506 194 1474 1384 268 1917 Fragments arranged by size: 1917 1474 1384 506 268 194 AfeI AGC'GCT Cuts at: 2345 2345 Size: 5743 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 2058 4472 2058 Size: 2414 3329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2733 1184 Size: 1549 4194 AlwNI CAG_nnn'CTG Cuts at: 2486 2671 2857 2899 3486 5058 2486 Size: 185 186 42 587 1572 3171 Fragments arranged by size: 3171 1572 587 186 185 42 ApaI G_GGCC'C Cuts at: 2018 2037 2149 2018 Size: 19 112 5612 Fragments arranged by size: 5612 112 19 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2102 2246 2436 3068 1870 Size: 73 86 73 144 190 632 4545 Fragments arranged by size: 4545 632 190 144 86 73 73 AvrII C'CTAG_G Cuts at: 2260 2260 Size: 5743 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 2189 3064 4519 618 Size: 325 217 627 310 92 875 1455 1842 Fragments arranged by size: 1842 1455 875 627 325 310 217 92 BanII G_rGCy'C Cuts at: 1391 2018 2037 2149 2641 2947 3062 1391 Size: 627 19 112 492 306 115 4072 Fragments arranged by size: 4072 627 492 306 115 112 19 BbeI G_GCGC'C Cuts at: 2101 2101 Size: 5743 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3016 928 Size: 971 1117 3655 Fragments arranged by size: 3655 1117 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2220 4354 136 Size: 107 122 71 631 605 548 2134 1525 Fragments arranged by size: 2134 1525 631 605 548 122 107 71 BglII A'GATC_T Cuts at: 3003 5738 3003 Size: 2735 3008 BlpI GC'TnA_GC Cuts at: 2961 3160 2961 Size: 199 5544 Bme1580I G_kGCm'C Cuts at: 1790 2018 2037 2149 3804 4960 1790 Size: 228 19 112 1655 1156 2573 Fragments arranged by size: 2573 1655 1156 228 112 19 BmgBI CAC'GTC Cuts at: 1318 2233 1318 Size: 915 4828 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2438 2864 4403 1239 Size: 986 213 426 1539 2579 Fragments arranged by size: 2579 1539 986 426 213 Bpu10I CC'TnA_GC Cuts at: 2580 2580 Size: 5743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2076 2264 3868 4733 5031 2076 Size: 188 1604 865 298 2788 Fragments arranged by size: 2788 1604 865 298 188 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2098 2223 3550 3932 Size: 53 83 186 936 565 125 1327 382 Cuts at: 3932 4567 275 Size: 635 1451 Fragments arranged by size: 1451 1327 936 635 565 382 186 125 83 53 BsaWI w'CCGG_w Cuts at: 2970 4176 4574 4848 4995 5406 2970 Size: 1206 398 274 147 411 3307 Fragments arranged by size: 3307 1206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 1178 Size: 30 165 30 743 30 4745 Fragments arranged by size: 4745 743 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3005 1186 Size: 1819 3924 BseYI C'CCAG_C Cuts at: 2448 2553 2924 4946 5442 2448 Size: 105 371 2022 496 2749 Fragments arranged by size: 2749 2022 496 371 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3002 1163 Size: 573 1266 3904 Fragments arranged by size: 3904 1266 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2081 2143 3193 3954 4982 1540 Size: 275 186 80 62 1050 761 1028 2301 Fragments arranged by size: 2301 1050 1028 761 275 186 80 62 BsiHKAI G_wGCw'C Cuts at: 2712 3804 3889 4960 2712 Size: 1092 85 1071 3495 Fragments arranged by size: 3495 1092 1071 85 BsmI GAATG_Cn' Cuts at: 2650 3254 3347 2650 Size: 604 93 5046 Fragments arranged by size: 5046 604 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2037 2149 2641 2712 2947 3062 Size: 399 228 19 112 492 71 235 115 Cuts at: 3062 3804 3889 4960 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 235 228 115 112 85 71 19 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2656 2974 3098 844 Size: 1812 318 124 3489 Fragments arranged by size: 3489 1812 318 124 BsrBI CCG'CTC Cuts at: 1394 1823 2162 3630 1394 Size: 429 339 1468 3507 Fragments arranged by size: 3507 1468 429 339 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 2622 3803 4815 2622 Size: 1181 1012 3550 Fragments arranged by size: 3550 1181 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2627 794 Size: 1833 3910 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2462 2462 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2199 2253 3003 3073 3440 3825 3842 5294 Size: 227 54 750 70 367 385 17 1452 Cuts at: 5294 5380 5392 5738 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 750 385 367 346 227 86 70 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2295 2318 2295 Size: 23 5720 BtgI C'CryG_G Cuts at: 513 1065 2167 513 Size: 552 1102 4089 Fragments arranged by size: 4089 1102 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2660 3259 4053 4073 2660 Size: 599 794 20 4330 Fragments arranged by size: 4330 794 599 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2730 2765 529 Size: 35 2166 35 3507 Fragments arranged by size: 3507 2166 35 35 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2078 2212 3190 Size: 54 1425 50 275 18 248 134 978 Cuts at: 3190 4079 5708 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 978 889 275 248 134 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2078 3190 1537 Size: 275 266 1112 4090 Fragments arranged by size: 4090 1112 275 266 EarI CTCTTCn'nnn_ Cuts at: 1353 3673 1353 Size: 2320 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2338 4330 4704 4850 387 Size: 1064 138 749 1992 374 146 1280 Fragments arranged by size: 1992 1280 1064 749 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2225 2332 2438 2864 3806 4403 Size: 393 506 87 107 106 426 942 597 Cuts at: 4403 5190 5458 1239 Size: 787 268 1524 Fragments arranged by size: 1524 942 787 597 506 426 393 268 107 106 87 EcoO109I rG'GnC_Cy Cuts at: 2014 2033 2146 2264 2271 2290 2388 2570 2668 Size: 19 113 118 7 19 98 182 98 Cuts at: 2668 2764 2014 Size: 96 4993 Fragments arranged by size: 4993 182 118 113 98 98 96 19 19 7 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2143 2143 Size: 5743 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2101 2347 2410 4890 1678 Size: 423 246 63 2480 2531 Fragments arranged by size: 2531 2480 423 246 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2044 2074 2076 Size: 32 135 32 431 32 9 30 2 Cuts at: 2076 2106 2995 3027 4384 4416 4458 4490 1373 Size: 30 889 32 1357 32 42 32 2626 Fragments arranged by size: 2626 1357 889 431 135 42 32 32 32 32 32 32 30 30 9 2 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KasI G'GCGC_C Cuts at: 2097 2097 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 2733 4857 5041 1305 Size: 858 570 2124 184 2007 Fragments arranged by size: 2124 2007 858 570 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2715 2837 3701 4060 4219 518 Size: 648 1549 122 864 359 159 2042 Fragments arranged by size: 2042 1549 864 648 359 159 122 MspA1I CmG'CkG Cuts at: 1606 2514 2695 2854 2896 3159 3836 4984 5229 Size: 908 181 159 42 263 677 1148 245 Cuts at: 5229 1606 Size: 2120 Fragments arranged by size: 2120 1148 908 677 263 245 181 159 42 NarI GG'CG_CC Cuts at: 2098 2098 Size: 5743 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5191 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2842 3101 4646 1132 Size: 97 1613 259 1545 2229 Fragments arranged by size: 2229 1613 1545 259 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2857 1108 Size: 1749 3994 PfoI T'CCnGG_A Cuts at: 2202 2454 2202 Size: 252 5491 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2264 2290 2570 2668 2764 2264 Size: 26 280 98 96 5243 Fragments arranged by size: 5243 280 98 96 26 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2014 2033 2145 2014 Size: 19 112 5612 Fragments arranged by size: 5612 112 19 PspXI vC'TCGA_Gb Cuts at: 2102 2246 2102 Size: 144 5599 PstI C_TGCA'G Cuts at: 830 2667 3095 830 Size: 1837 428 3478 Fragments arranged by size: 3478 1837 428 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2896 2896 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SexAI A'CCwGG_T Cuts at: 2566 2566 Size: 5743 SfcI C'TryA_G Cuts at: 826 1045 2071 2663 3091 4226 4907 5098 826 Size: 219 1026 592 428 1135 681 191 1471 Fragments arranged by size: 1471 1135 1026 681 592 428 219 191 SfoI GGC'GCC Cuts at: 2099 2099 Size: 5743 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2438 3070 1872 Size: 566 632 4545 Fragments arranged by size: 4545 632 566 SmlI C'TyrA_G Cuts at: 820 1017 1943 2091 2102 2246 2279 3847 4748 Size: 197 926 148 11 144 33 1568 901 Cuts at: 4748 5010 820 Size: 262 1553 Fragments arranged by size: 1568 1553 926 901 262 197 148 144 33 11 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 2842 3101 1132 Size: 1710 259 3774 Fragments arranged by size: 3774 1710 259 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 2592 1517 Size: 1075 4668 StyI C'CwwG_G Cuts at: 513 1065 2260 3182 513 Size: 552 1195 922 3074 Fragments arranged by size: 3074 1195 922 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 2428 3770 3787 3940 4125 4570 Size: 1255 591 257 1342 17 153 185 445 Cuts at: 4570 325 Size: 1498 Fragments arranged by size: 1498 1342 1255 591 445 257 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3989 5701 96 Size: 274 80 33 51 1443 2012 1712 138 Fragments arranged by size: 2012 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2146 2178 2652 2684 5703 5735 2146 Size: 32 474 32 3019 32 2154 Fragments arranged by size: 3019 2154 474 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2482 1316 Size: 1166 4577 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 2102 2246 1943 Size: 159 144 5440 Fragments arranged by size: 5440 159 144 XmaI C'CCGG_G Cuts at: 1870 2436 3068 1870 Size: 566 632 4545 Fragments arranged by size: 4545 632 566 XmnI GAAnn'nnTTC Cuts at: 2345 3872 2345 Size: 1527 4216 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI