Restriction map for
pFN21AE0007
(Circular) (Six-base) MAPSORT of: pFN21AE0007.seq Check: 7171 from: 1 to: 6259
pFN21AE0007 6259 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4069 278
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3602 1937
Size: 1665 4594
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4322 5706 5974 1632
Size: 2690 1384 268 1917
Fragments arranged by size:
2690 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 5158 6135 1203
Size: 3955 977 1327
Fragments arranged by size:
3955 1327 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 2819 4988 2819
Size: 2169 4090
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2344 3419 1184
Size: 1160 1075 4024
Fragments arranged by size:
4024 1160 1075
AlwNI CAG_nnn'CTG
Cuts at: 4002 5574 4002
Size: 1572 4687
ApaLI G'TGCA_C
Cuts at: 4316 5472 4316
Size: 1156 5103
ApoI r'AATT_y
Cuts at: 1493 2150 2479 2893 3568 3794 3946 6055 1493
Size: 657 329 414 675 226 152 2109 1697
Fragments arranged by size:
2109 1697 675 657 414 329 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 3584 1870
Size: 73 1641 4545
Fragments arranged by size:
4545 1641 73
BamHI G'GATC_C
Cuts at: 3589 3956 3589
Size: 367 5892
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3479 3580 5035 618
Size: 325 217 627 1692 101 1455 1842
Fragments arranged by size:
1842 1692 1455 627 325 217 101
BanII G_rGCy'C
Cuts at: 1391 2493 3578 1391
Size: 1102 1085 4072
Fragments arranged by size:
4072 1102 1085
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2677 928
Size: 971 778 4510
Fragments arranged by size:
4510 971 778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4450 4484 1903
Size: 34 50 34 2429 34 3678
Fragments arranged by size:
3678 2429 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2041 4151 5361 2041
Size: 2110 1210 2939
Fragments arranged by size:
2939 2110 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4870 136
Size: 107 122 71 631 605 3198 1525
Fragments arranged by size:
3198 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 3676 3676
Size: 6259
Bme1580I G_kGCm'C
Cuts at: 1790 4320 5476 1790
Size: 2530 1156 2573
Fragments arranged by size:
2573 2530 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2795 3990 4948
Size: 416 53 83 399 24 1378 1195 958
Cuts at: 4948 442
Size: 1753
Fragments arranged by size:
1753 1378 1195 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2477 4919 1239
Size: 1238 2442 2579
Fragments arranged by size:
2579 2442 1238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 2776 3446 4384 5249 5547 2636
Size: 140 670 938 865 298 3348
Fragments arranged by size:
3348 938 865 670 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4922 882
Size: 617 3423 2219
Fragments arranged by size:
3423 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4050 493
Size: 711 2846 2702
Fragments arranged by size:
2846 2702 711
BsaBI GATnn'nnATC
Cuts at: 6253 6253
Size: 6259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4066 4448 5083 275
Size: 53 83 186 936 2533 382 635 1451
Fragments arranged by size:
2533 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4692 5090 5364 5511 5922 4692
Size: 398 274 147 411 5029
Fragments arranged by size:
5029 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6034
Fragments arranged by size:
6034 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2501 1186
Size: 1315 4944
BseYI C'CCAG_C
Cuts at: 3230 3453 5462 5958 3230
Size: 223 2009 496 3531
Fragments arranged by size:
3531 2009 496 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5686
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3709 4470 5498 1540
Size: 275 186 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2493 2501 4320 4405 5476 2127
Size: 366 8 1819 85 1071 2910
Fragments arranged by size:
2910 1819 1071 366 85 8
BsmI GAATG_Cn'
Cuts at: 2519 3012 3398 3431 3770 3863 2519
Size: 493 386 33 339 93 4915
Fragments arranged by size:
4915 493 386 339 93 33
BsmBI CGTCTCn'nnnn_
Cuts at: 2531 3404 2531
Size: 873 5386
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2493 2501 3578 4320 4405 5476
Size: 399 337 366 8 1077 742 85 1071
Cuts at: 5476 1391
Size: 2174
Fragments arranged by size:
2174 1077 1071 742 399 366 337 85
8
BspEI T'CCGG_A
Cuts at: 5922 5922
Size: 6259
BspHI T'CATG_A
Cuts at: 3048 4148 5878 3048
Size: 1100 1730 3429
Fragments arranged by size:
3429 1730 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2270 3366 3614 844
Size: 1426 1096 248 3489
Fragments arranged by size:
3489 1426 1096 248
BsrBI CCG'CTC
Cuts at: 1394 1823 3335 4146 1394
Size: 429 1512 811 3507
Fragments arranged by size:
3507 1512 811 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4754 4928 65
Size: 1183 3506 174 1396
Fragments arranged by size:
3506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3476 4903 5090 1700
Size: 110 1666 1427 187 2869
Fragments arranged by size:
2869 1666 1427 187 110
BsrGI T'GTAC_A
Cuts at: 96 2593 2677 3250 96
Size: 2497 84 573 3105
Fragments arranged by size:
3105 2497 573 84
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6259
BssSI C'ACGA_G
Cuts at: 2203 4319 5331 2203
Size: 2116 1012 3131
Fragments arranged by size:
3131 2116 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3007 794
Size: 2213 4046
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstXI CCAn_nnnn'nTGG
Cuts at: 2818 2818
Size: 6259
BstYI r'GATC_y
Cuts at: 1972 3589 3956 4341 4358 5810 5896 5908 6254
Size: 1617 367 385 17 1452 86 12 346
Cuts at: 6254 1972
Size: 1977
Fragments arranged by size:
1977 1617 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2101 2944 3086 513
Size: 552 1036 843 142 3686
Fragments arranged by size:
3686 1036 843 552 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5520
Fragments arranged by size:
5520 683 56
BtsI GCAGTG_nn'
Cuts at: 2378 3398 3775 4569 4589 2378
Size: 1020 377 794 20 4048
Fragments arranged by size:
4048 1020 794 377 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6224
DraI TTT'AAA
Cuts at: 2538 3563 2538
Size: 1025 5234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2659 3468
Size: 54 1425 50 275 18 300 529 809
Cuts at: 3468 3706 4595 6224 8
Size: 238 889 1629 43
Fragments arranged by size:
1629 1425 889 809 529 300 275 238
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3706 1537
Size: 275 1894 4090
Fragments arranged by size:
4090 1894 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4189 1353
Size: 2836 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4846 5220 5366 387
Size: 1064 138 3257 374 146 1280
Fragments arranged by size:
3257 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2491 2491
Size: 6259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2477 4322 4919 5706 5974 1239
Size: 393 845 1845 597 787 268 1524
Fragments arranged by size:
1845 1524 845 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2990 3113 2990
Size: 123 6136
EcoRI G'AATT_C
Cuts at: 3568 3946 3568
Size: 378 5881
EcoRV GAT'ATC
Cuts at: 2788 2788
Size: 6259
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 5406 1678
Size: 3728 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2805 2837 4900 4932 4974
Size: 32 135 32 1233 32 2063 32 42
Cuts at: 4974 5006 1373
Size: 32 2626
Fragments arranged by size:
2626 2063 1233 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3603 3849 669
Size: 988 281 1665 246 3079
Fragments arranged by size:
3079 1665 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2144 748
Size: 1396 4863
HpaI GTT'AAC
Cuts at: 3849 3849
Size: 6259
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5373 5557 1305
Size: 4068 184 2007
Fragments arranged by size:
4068 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 2661 3470 10
Size: 54 1425 343 300 529 809 2799
Fragments arranged by size:
2799 1425 809 529 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2471 3423 4217 4576 4735 518
Size: 648 1305 952 794 359 159 2042
Fragments arranged by size:
2042 1305 952 794 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3234 3439 3675 4352 5500 5745 1606
Size: 1628 205 236 677 1148 245 2120
Fragments arranged by size:
2120 1628 1148 677 245 236 205
NcoI C'CATG_G
Cuts at: 513 1065 2101 2944 3086 513
Size: 552 1036 843 142 3686
Fragments arranged by size:
3686 1036 843 552 142
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 3382 3617 5162 1132
Size: 97 2153 235 1545 2229
Fragments arranged by size:
2229 2153 1545 235 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6259
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6259
PfoI T'CCnGG_A
Cuts at: 3351 3351
Size: 6259
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4328 4360 5841 5873 4328
Size: 32 1481 32 4714
Fragments arranged by size:
4714 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2990 3113 2990
Size: 123 6136
PshAI GACnn'nnGTC
Cuts at: 1613 2053 2355 1613
Size: 440 302 5517
Fragments arranged by size:
5517 440 302
PsiI TTA'TAA
Cuts at: 2214 3829 2214
Size: 1615 4644
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2964 2996 2964
Size: 32 6227
PstI C_TGCA'G
Cuts at: 830 3180 3243 3611 830
Size: 2350 63 368 3478
Fragments arranged by size:
3478 2350 368 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
PvuII CAG'CTG
Cuts at: 3439 3439
Size: 6259
SacI G_AGCT'C
Cuts at: 2493 2493
Size: 6259
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SbfI CC_TGCA'GG
Cuts at: 3611 3611
Size: 6259
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 2222 2829 3176 3239 3607 4742 5423
Size: 219 1177 607 347 63 368 1135 681
Cuts at: 5423 5614 826
Size: 191 1471
Fragments arranged by size:
1471 1177 1135 681 607 368 347 219
191 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 3586 1872
Size: 1714 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2651 2791 3461 4363 5264 5526
Size: 197 926 708 140 670 902 901 262
Cuts at: 5526 820
Size: 1553
Fragments arranged by size:
1553 926 902 901 708 670 262 197
140
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 3617 1132
Size: 2485 3774
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6259
StyI C'CwwG_G
Cuts at: 513 1065 2101 2508 2944 3086 3698 513
Size: 552 1036 407 436 142 612 3074
Fragments arranged by size:
3074 1036 612 552 436 407 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4286 4303 4456 4641 5086 325
Size: 1255 2706 17 153 185 445 1498
Fragments arranged by size:
2706 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2593 2677 3250
Size: 274 80 33 51 1443 616 84 573
Cuts at: 3250 4505 6217 96
Size: 1255 1712 138
Fragments arranged by size:
1712 1443 1255 616 573 274 138 84
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6259
XbaI T'CTAG_A
Cuts at: 3595 3595
Size: 6259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6259
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6259
XmaI C'CCGG_G
Cuts at: 1870 3584 1870
Size: 1714 4545
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4067 276
Size: 53 83 186 936 2533 2468
Fragments arranged by size:
2533 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI