Restriction map for
pFN21AB7340
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# NO CUT ENZYMES ( 77 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BfrI BglI BglII BsiWI
Bsp1407I BspDI BspHI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI Eam1105I Eco81I EcoO65I EcoRV
FseI FspI KpnI MfeI MluI
MunI NcoI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 3447 ACATGT A^C[GA][CT]GT
Aor51HI 940 AGCGCT AGC^GCT
Asp700I 915 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 867 CCTAGG C^CTAGG
BbeI 67 GGCGCC GGCGC^C
BbrPI 389 CACGTG CAC^GTG
BbsI 285 GAAGAC GAAGAC(2/6)
BciVI 1734 GGATAC GTATCC(6/5)
BclI 1422 TGATCA T^GATCA
BlnI 867 CCTAGG C^CTAGG
BpuAI 285 GAAGAC GAAGAC(2/6)
BsaAI 389 CACGTG [CT]AC^GT[GA]
BsaBI 487 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1363 GCATTC GAATGC(1/-1)
BspLU11I 3447 ACATGT A^CATGT
BspMI 1059 ACCTGC ACCTGC(4/8)
BsrBI 74 CCGCTC CCGCTC(-3/-3)
DsaI 141 CCGCGG C^C[GA][CT]GG
EagI 233 CGGCCG C^GGCCG
EclXI 233 CGGCCG C^GGCCG
Eco47III 940 AGCGCT AGC^GCT
Eco52I 233 CGGCCG C^GGCCG
EcoNI 974 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2098 ATGCAT ATGCA^T
FbaI 1422 TGATCA T^GATCA
HincII 2841 GTTAAC GT[CT]^[GA]AC
HindII 2841 GTTAAC GT[CT]^[GA]AC
HindIII 1973 AAGCTT A^AGCTT
HpaI 2841 GTTAAC GTT^AAC
KasI 67 GGCGCC G^GCGCC
KspI 141 CCGCGG CCGC^GG
MamI 487 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 67 GGCGCC GG^CGCC
NdeI 3434 CATATG CA^TATG
NheI 3217 GCTAGC G^CTAGC
NotI 232 GCGGCCGC GC^GGCCGC
NsiI 2098 ATGCAT ATGCA^T
PmaCI 389 CACGTG CAC^GTG
PmlI 389 CACGTG CAC^GTG
PshAI 1848 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 141 CCGCGG CCGC^GG
SexAI 2826 ACCAGGT A^CC[AT]GGT
SfoI 67 GGCGCC GGC^GCC
StuI 172 AGGCCT AGG^CCT
XmnI 915 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 131 CGCG CG^CG
142 CGCG
AccIII 768 TCCGGA T^CCGGA
892 TCCGGA
AspHI 690 GAGCTC G[AT]GC[AT]^C
1791 GAGCTC
BalI 275 TGGCCA TGG^CCA
3186 TGGCCA
BbvCI 1933 CCTCAGC CCTCAGC(-5/-2)
2600 CCTCAGC
BcgI 992 CGATTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
188 GCATCTTCGTCG
BlpI 1063 GCTAAGC GC^T[ACGT]AGC
2881 GCTGAGC
Bpu1102I 1063 GCTAAGC GC^T[ACGT]AGC
2881 GCTGAGC
BseAI 768 TCCGGA T^CCGGA
892 TCCGGA
BsiEI 105 CGGTCG CG[GA][CT]^CG
233 CGGCCG
BsiHKAI 690 GAGCTC G[AT]GC[AT]^C
1791 GAGCTC
BsmBI 680 CGTCTC CGTCTC(1/5)
587 GAGACG
BspEI 768 TCCGGA T^CCGGA
892 TCCGGA
BstUI 131 CGCG CG^CG
142 CGCG
CelII 1063 GCTAAGC GC^T[ACGT]AGC
2881 GCTGAGC
DraI 2289 TTTAAA TTT^AAA
2670 TTTAAA
DraIII 386 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
2804 CACTGAGTG
EcoRI 1644 GAATTC G^AATTC
3288 GAATTC
HgaI 91 GACGC GACGC(5/10)
679 GCGTC
MluNI 275 TGGCCA TGG^CCA
3186 TGGCCA
MroI 768 TCCGGA T^CCGGA
892 TCCGGA
MscI 275 TGGCCA TGG^CCA
3186 TGGCCA
MvnI 131 CGCG CG^CG
142 CGCG
SacI 690 GAGCTC GAGCT^C
1791 GAGCTC
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI