(Circular) (Six-base) MAPSORT of: pFN21AB7340.seq Check: 4447 from: 1 to: 8173 pFN21AB7340 8173 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3073 3073 Size: 8173 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5983 278 Size: 53 83 186 936 4447 2468 Fragments arranged by size: 4447 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2640 3717 3861 4517 5516 1937 Size: 703 1077 144 656 999 4594 Fragments arranged by size: 4594 1077 999 703 656 144 Acc65I G'GTAC_C Cuts at: 5494 5494 Size: 8173 AclI AA'CG_TT Cuts at: 1563 6300 6673 1563 Size: 4737 373 3063 Fragments arranged by size: 4737 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 2590 3744 4782 6236 7620 7888 1632 Size: 534 424 1154 1038 1454 1384 268 1917 Fragments arranged by size: 1917 1454 1384 1154 1038 534 424 268 AfeI AGC'GCT Cuts at: 2947 2947 Size: 8173 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7976 AflIII A'CryG_T Cuts at: 1203 5452 7072 8049 1203 Size: 4249 1620 977 1327 Fragments arranged by size: 4249 1620 1327 977 AgeI A'CCGG_T Cuts at: 7004 7004 Size: 8173 AhdI GACnn_n'nnGTC Cuts at: 6902 6902 Size: 8173 AleI CACnn'nnGTG Cuts at: 1166 4046 4421 1166 Size: 2880 375 4918 Fragments arranged by size: 4918 2880 375 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8173 AlwNI CAG_nnn'CTG Cuts at: 2979 3996 4810 5916 7488 2979 Size: 1017 814 1106 1572 3664 Fragments arranged by size: 3664 1572 1106 1017 814 ApaI G_GGCC'C Cuts at: 2464 2475 4333 2464 Size: 11 1858 6304 Fragments arranged by size: 6304 1858 11 ApaLI G'TGCA_C Cuts at: 6230 7386 6230 Size: 1156 7017 ApoI r'AATT_y Cuts at: 1493 2024 3649 3808 3947 4322 5293 5399 5482 Size: 531 1625 159 139 375 971 106 83 Cuts at: 5482 5708 5860 7969 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 971 531 375 226 159 152 139 106 83 AseI AT'TA_AT Cuts at: 160 1032 6727 160 Size: 872 5695 1606 Fragments arranged by size: 5695 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8173 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2091 2229 2320 3844 5498 1870 Size: 73 90 58 138 91 1524 1654 4545 Fragments arranged by size: 4545 1654 1524 138 91 90 73 58 AvrII C'CTAG_G Cuts at: 2872 2872 Size: 8173 BamHI G'GATC_C Cuts at: 2349 2497 4685 5503 5870 2349 Size: 148 2188 818 367 4652 Fragments arranged by size: 4652 2188 818 367 148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 2072 2582 3840 4267 Size: 325 217 627 250 35 510 1258 427 Cuts at: 4267 5321 5494 6949 618 Size: 1054 173 1455 1842 Fragments arranged by size: 1842 1455 1258 1054 627 510 427 325 250 217 173 35 BanII G_rGCy'C Cuts at: 1391 2119 2464 2475 2699 3122 3800 4333 5492 Size: 728 345 11 224 423 678 533 1159 Cuts at: 5492 1391 Size: 4072 Fragments arranged by size: 4072 1159 728 678 533 423 345 224 11 BbeI G_GCGC'C Cuts at: 2076 2076 Size: 8173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2297 928 Size: 971 398 6804 Fragments arranged by size: 6804 971 398 BbvCI CC'TCA_GC Cuts at: 3939 4606 3939 Size: 667 7506 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2182 2216 2986 3020 6364 6398 1903 Size: 34 245 34 770 34 3344 34 3678 Fragments arranged by size: 3678 3344 770 245 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3733 6065 7275 3733 Size: 2332 1210 4631 Fragments arranged by size: 4631 2332 1210 BclI T'GATC_A Cuts at: 1548 3427 1548 Size: 1879 6294 BfrBI ATG'CAT Cuts at: 4105 4105 Size: 8173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6784 136 Size: 107 122 71 631 605 5112 1525 Fragments arranged by size: 5112 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8168 8168 Size: 8173 BlpI GC'TnA_GC Cuts at: 3069 4887 5590 3069 Size: 1818 703 5652 Fragments arranged by size: 5652 1818 703 Bme1580I G_kGCm'C Cuts at: 1790 2464 2475 2585 2752 4270 4333 4372 4874 Size: 674 11 110 167 1518 63 39 502 Cuts at: 4874 6234 7390 1790 Size: 1360 1156 2573 Fragments arranged by size: 2573 1518 1360 1156 674 502 167 110 63 39 11 BmgBI CAC'GTC Cuts at: 1318 2596 3657 1318 Size: 1278 1061 5834 Fragments arranged by size: 5834 1278 1061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2331 3984 4359 Size: 416 53 83 399 24 914 1653 375 Cuts at: 4359 4971 5085 5904 6862 442 Size: 612 114 819 958 1753 Fragments arranged by size: 1753 1653 958 914 819 612 416 399 375 114 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5226 1056 Size: 4170 4003 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2967 2967 Size: 8173 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 2648 2673 3772 4014 4389 4513 4998 Size: 1223 186 25 1099 242 375 124 485 Cuts at: 4998 6833 1239 Size: 1835 2579 Fragments arranged by size: 2579 1835 1223 1099 485 375 242 186 124 25 Bpu10I CC'TnA_GC Cuts at: 2543 2793 3782 3939 4007 4382 4606 2543 Size: 250 989 157 68 375 224 6110 Fragments arranged by size: 6110 989 375 250 224 157 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6298 7163 7461 6298 Size: 865 298 7010 Fragments arranged by size: 7010 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2441 3090 3158 3268 4642 6836 882 Size: 617 942 649 68 110 1374 2194 2219 Fragments arranged by size: 2219 2194 1374 942 649 617 110 68 BsaAI yAC'GTr Cuts at: 493 1204 2396 5964 493 Size: 711 1192 3568 2702 Fragments arranged by size: 3568 2702 1192 711 BsaBI GATnn'nnATC Cuts at: 2496 8167 2496 Size: 5671 2502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2097 2684 5980 Size: 53 83 186 936 540 24 587 3296 Cuts at: 5980 6362 6997 275 Size: 382 635 1451 Fragments arranged by size: 3296 1451 936 635 587 540 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 2500 2773 2897 5470 6606 7004 7278 7425 7836 Size: 273 124 2573 1136 398 274 147 411 Cuts at: 7836 2500 Size: 2837 Fragments arranged by size: 2837 2573 1136 411 398 274 273 147 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2585 2615 4099 4129 4474 Size: 30 165 30 1182 30 1484 30 345 Cuts at: 4474 4504 1178 Size: 30 4847 Fragments arranged by size: 4847 1484 1182 345 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2346 2459 2943 3378 3567 3570 4017 4104 Size: 1160 113 484 435 189 3 447 87 Cuts at: 4104 4553 1186 Size: 449 4806 Fragments arranged by size: 4806 1160 484 449 447 435 189 113 87 3 BseYI C'CCAG_C Cuts at: 2360 2428 2723 2864 3189 4208 4768 5141 7376 Size: 68 295 141 325 1019 560 373 2235 Cuts at: 7376 7872 2360 Size: 496 2661 Fragments arranged by size: 2661 2235 1019 560 496 373 325 295 141 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2525 3239 3849 4236 4617 5193 1163 Size: 573 789 714 610 387 381 576 4143 Fragments arranged by size: 4143 789 714 610 576 573 387 381 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2113 2241 5623 6384 7412 1540 Size: 275 186 112 128 3382 761 1028 2301 Fragments arranged by size: 3382 2301 1028 761 275 186 128 112 BsiHKAI G_wGCw'C Cuts at: 2699 3800 6234 6319 7390 2699 Size: 1101 2434 85 1071 3482 Fragments arranged by size: 3482 2434 1101 1071 85 BsmI GAATG_Cn' Cuts at: 3368 5684 5777 3368 Size: 2316 93 5764 Fragments arranged by size: 5764 2316 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2586 2691 2586 Size: 105 8068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 2464 2475 2585 2699 2752 3122 Size: 399 329 345 11 110 114 53 370 Cuts at: 3122 3800 4270 4333 4372 4874 5492 6234 6319 Size: 678 470 63 39 502 618 742 85 Cuts at: 6319 7390 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 678 618 502 470 399 370 345 329 114 110 85 63 53 39 11 BspEI T'CCGG_A Cuts at: 2773 2897 7836 2773 Size: 124 4939 3110 Fragments arranged by size: 4939 3110 124 BspHI T'CATG_A Cuts at: 6062 7792 6062 Size: 1730 6443 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3073 5528 844 Size: 2229 2455 3489 Fragments arranged by size: 3489 2455 2229 BsrBI CCG'CTC Cuts at: 1394 1823 2081 6060 1394 Size: 429 258 3979 3507 Fragments arranged by size: 3979 3507 429 258 BsrDI GCAATG_nn' Cuts at: 65 1248 3002 3032 3820 4599 6668 6842 65 Size: 1183 1754 30 788 779 2069 174 1396 Fragments arranged by size: 2069 1754 1396 1183 788 779 174 30 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 2260 2401 6817 7004 1700 Size: 110 236 214 141 4416 187 2869 Fragments arranged by size: 4416 2869 236 214 187 141 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8173 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8173 BssSI C'ACGA_G Cuts at: 6233 7245 6233 Size: 1012 7161 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8173 BstBI TT'CG_AA Cuts at: 6977 6977 Size: 8173 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8173 BstXI CCAn_nnnn'nTGG Cuts at: 2440 3039 3920 5426 2440 Size: 599 881 1506 5187 Fragments arranged by size: 5187 1506 881 599 BstYI r'GATC_y Cuts at: 1972 2349 2497 4685 5327 5503 5870 6255 6272 Size: 377 148 2188 642 176 367 385 17 Cuts at: 6272 7724 7810 7822 8168 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2188 1977 1452 642 385 377 367 346 176 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2146 513 Size: 552 939 142 6540 Fragments arranged by size: 6540 939 552 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7434 Fragments arranged by size: 7434 683 56 BtsI GCAGTG_nn' Cuts at: 3026 3832 4218 4435 5308 5689 6483 6503 3026 Size: 806 386 217 873 381 794 20 4696 Fragments arranged by size: 4696 873 806 794 386 381 217 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8173 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8138 DraI TTT'AAA Cuts at: 4296 4677 5477 4296 Size: 381 800 6992 Fragments arranged by size: 6992 800 381 DraIII CAC_nnn'GTG Cuts at: 2396 4814 2396 Size: 2418 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7180 809 Size: 507 5864 1802 Fragments arranged by size: 5864 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2238 2280 5191 Size: 54 1425 50 275 18 408 42 2911 Cuts at: 5191 5620 6509 8138 8 Size: 429 889 1629 43 Fragments arranged by size: 2911 1629 1425 889 429 408 275 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2238 5620 1537 Size: 275 426 3382 4090 Fragments arranged by size: 4090 3382 426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2473 2847 3110 3402 3603 6103 1353 Size: 1120 374 263 292 201 2500 3423 Fragments arranged by size: 3423 2500 1120 374 292 263 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2147 6760 7134 7280 387 Size: 1064 138 558 4613 374 146 1280 Fragments arranged by size: 4613 1280 1064 558 374 146 138 EcoICRI GAG'CTC Cuts at: 2697 3798 2697 Size: 1101 7072 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2462 2590 2648 2673 3744 3772 Size: 393 534 296 128 58 25 1071 28 Cuts at: 3772 4014 4389 4513 4782 4998 6236 6833 7620 Size: 242 375 124 269 216 1238 597 787 Cuts at: 7620 7888 1239 Size: 268 1524 Fragments arranged by size: 1524 1238 1071 787 597 534 393 375 296 269 268 242 216 128 124 58 28 25 EcoNI CCTnn'n_nnAGG Cuts at: 2983 2983 Size: 8173 EcoO109I rG'GnC_Cy Cuts at: 2254 2471 2535 2574 2711 2976 3621 4329 4330 Size: 217 64 39 137 265 645 708 1 Cuts at: 4330 5033 5087 5182 5342 2254 Size: 703 54 95 160 5085 Fragments arranged by size: 5085 708 703 645 265 217 160 137 95 64 54 39 1 EcoRI G'AATT_C Cuts at: 3649 5293 5482 5860 3649 Size: 1644 189 378 5962 Fragments arranged by size: 5962 1644 378 189 FspI TGC'GCA Cuts at: 6679 6679 Size: 8173 HaeII r_GCGC'y Cuts at: 1678 2076 2789 2949 3479 7320 1678 Size: 398 713 160 530 3841 2531 Fragments arranged by size: 3841 2531 713 530 398 160 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2203 2235 2341 2373 2585 Size: 32 135 32 631 32 106 32 212 Cuts at: 2585 2617 3300 3332 4835 4867 6814 6846 6888 Size: 32 683 32 1503 32 1947 32 42 Cuts at: 6888 6920 1373 Size: 32 2626 Fragments arranged by size: 2626 1947 1503 683 631 212 135 106 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4848 5517 5763 669 Size: 988 281 2910 669 246 3079 Fragments arranged by size: 3079 2910 988 669 281 246 HindIII A'AGCT_T Cuts at: 748 3978 748 Size: 3230 4943 HpaI GTT'AAC Cuts at: 4848 5763 4848 Size: 915 7258 KasI G'GCGC_C Cuts at: 2072 2072 Size: 8173 KpnI G_GTAC'C Cuts at: 5498 5498 Size: 8173 MfeI C'AATT_G Cuts at: 5772 5772 Size: 8173 MluI A'CGCG_T Cuts at: 8049 8049 Size: 8173 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4308 4978 5317 5488 7287 7471 1305 Size: 3003 670 339 171 1799 184 2007 Fragments arranged by size: 3003 2007 1799 670 339 184 171 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 5193 10 Size: 54 1425 343 450 2911 2990 Fragments arranged by size: 2990 2911 1425 450 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3037 3825 4046 4421 4964 5424 6131 Size: 648 1871 788 221 375 543 460 707 Cuts at: 6131 6490 6649 518 Size: 359 159 2042 Fragments arranged by size: 2042 1871 788 707 648 543 460 375 359 221 159 MspA1I CmG'CkG Cuts at: 1606 2148 2550 2890 3016 4212 5589 6266 7414 Size: 542 402 340 126 1196 1377 677 1148 Cuts at: 7414 7659 1606 Size: 245 2120 Fragments arranged by size: 2120 1377 1196 1148 677 542 402 340 245 126 NaeI GCC'GGC Cuts at: 2048 2262 2403 2048 Size: 214 141 7818 Fragments arranged by size: 7818 214 141 NarI GG'CG_CC Cuts at: 2073 2073 Size: 8173 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6682 Fragments arranged by size: 6682 939 552 NdeI CA'TA_TG Cuts at: 387 5440 387 Size: 5053 3120 NgoMIV G'CCGG_C Cuts at: 2046 2260 2401 2046 Size: 214 141 7818 Fragments arranged by size: 7818 214 141 NheI G'CTAG_C Cuts at: 1052 5222 1052 Size: 4170 4003 NotI GC'GGCC_GC Cuts at: 2238 5620 2238 Size: 3382 4791 NsiI A_TGCA'T Cuts at: 4107 4107 Size: 8173 NspI r_CATG'y Cuts at: 1132 1229 2371 5077 5456 5531 7076 1132 Size: 97 1142 2706 379 75 1545 2229 Fragments arranged by size: 2706 2229 1545 1142 379 97 75 PasI CC'CwG_GG Cuts at: 1348 2578 2587 2618 1348 Size: 1230 9 31 6903 Fragments arranged by size: 6903 1230 31 9 PciI A'CATG_T Cuts at: 5452 7072 5452 Size: 1620 6553 PflMI CCAn_nnn'nTGG Cuts at: 1108 2069 2804 4814 5200 1108 Size: 961 735 2010 386 4081 Fragments arranged by size: 4081 2010 961 735 386 PfoI T'CCnGG_A Cuts at: 2439 4300 4529 2439 Size: 1861 229 6083 Fragments arranged by size: 6083 1861 229 PmeI GTTT'AAAC Cuts at: 5477 5477 Size: 8173 PmlI CAC'GTG Cuts at: 2396 2396 Size: 8173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 6242 6274 7755 7787 4043 Size: 32 2167 32 1481 32 4429 Fragments arranged by size: 4429 2167 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2535 2574 3621 5033 5182 2254 Size: 281 39 1047 1412 149 5245 Fragments arranged by size: 5245 1412 1047 281 149 39 PshAI GACnn'nnGTC Cuts at: 1613 3857 1613 Size: 2244 5929 PsiI TTA'TAA Cuts at: 3456 5743 3456 Size: 2287 5886 PspOMI G'GGCC_C Cuts at: 2460 2471 4329 2460 Size: 11 1858 6304 Fragments arranged by size: 6304 1858 11 PstI C_TGCA'G Cuts at: 830 2823 4441 5379 5525 830 Size: 1993 1618 938 146 3478 Fragments arranged by size: 3478 1993 1618 938 146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8173 SacI G_AGCT'C Cuts at: 2699 3800 2699 Size: 1101 7072 SacII CC_GC'GG Cuts at: 2149 2149 Size: 8173 SalI G'TCGA_C Cuts at: 1936 5515 1936 Size: 3579 4594 SanDI GG'GwC_CC Cuts at: 5182 5182 Size: 8173 SapI GCTCTTCn'nnn_ Cuts at: 2847 3110 3402 2847 Size: 263 292 7618 Fragments arranged by size: 7618 292 263 SbfI CC_TGCA'GG Cuts at: 5525 5525 Size: 8173 ScaI AGT'ACT Cuts at: 6421 8133 6421 Size: 1712 6461 SexAI A'CCwGG_T Cuts at: 4831 4831 Size: 8173 SfcI C'TryA_G Cuts at: 826 1045 2819 4062 4275 4437 5375 5521 6656 Size: 219 1774 1243 213 162 938 146 1135 Cuts at: 6656 7337 7528 826 Size: 681 191 1471 Fragments arranged by size: 1774 1471 1243 1135 938 681 219 213 191 162 146 SfoI GGC'GCC Cuts at: 2074 2074 Size: 8173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8173 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8173 SmaI CCC'GGG Cuts at: 1872 2035 2093 3846 5500 1872 Size: 163 58 1753 1654 4545 Fragments arranged by size: 4545 1753 1654 163 58 SmlI C'TyrA_G Cuts at: 820 1017 1943 6277 7178 7440 820 Size: 197 926 4334 901 262 1553 Fragments arranged by size: 4334 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5964 493 Size: 5471 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8173 SphI G_CATG'C Cuts at: 1132 5531 1132 Size: 4399 3774 SspI AAT'ATT Cuts at: 5 52 6097 5 Size: 47 6045 2081 Fragments arranged by size: 6045 2081 47 StuI AGG'CCT Cuts at: 1517 2179 1517 Size: 662 7511 StyI C'CwwG_G Cuts at: 513 1065 2004 2872 2939 3108 4122 4231 5612 Size: 552 939 868 67 169 1014 109 1381 Cuts at: 5612 513 Size: 3074 Fragments arranged by size: 3074 1381 1014 939 868 552 169 109 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2311 2465 2600 3203 3413 5155 5432 Size: 1255 731 154 135 603 210 1742 277 Cuts at: 5432 6200 6217 6370 6555 7000 325 Size: 768 17 153 185 445 1498 Fragments arranged by size: 1742 1498 1255 768 731 603 445 277 210 185 154 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4542 6419 8131 Size: 274 80 33 51 1443 2565 1877 1712 Cuts at: 8131 96 Size: 138 Fragments arranged by size: 2565 1877 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2324 2356 4612 4644 8133 8165 2324 Size: 32 2256 32 3489 32 2332 Fragments arranged by size: 3489 2332 2256 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8173 XbaI T'CTAG_A Cuts at: 5509 5509 Size: 8173 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3286 3349 4811 3286 Size: 63 1462 6648 Fragments arranged by size: 6648 1462 63 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8173 XmaI C'CCGG_G Cuts at: 1870 2033 2091 3844 5498 1870 Size: 163 58 1753 1654 4545 Fragments arranged by size: 4545 1753 1654 163 58 XmnI GAAnn'nnTTC Cuts at: 2924 6302 2924 Size: 3378 4795 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5981 276 Size: 53 83 186 936 4447 2468 Fragments arranged by size: 4447 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI PacI PspXI PsrI PvuII RsrII SfiI SrfI SwaI