Restriction map for
pFN21AB7340
(Linear) (Six-base) MAPSORT of: KIBB7340.seq Check: 7702 from: 1 to: 3468
KIBB7340.orf
With 182 enzymes: SgfI *
August 20, 2009 16:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1068 3468
Size: 1068 2400
AccI GT'mk_AC
Cuts at: 0 635 1712 1856 2512 3468
Size: 635 1077 144 656 956
Fragments arranged by size:
1077 956 656 635 144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 585 1739 2777 3468
Size: 161 424 1154 1038 691
Fragments arranged by size:
1154 1038 691 424 161
AfeI AGC'GCT
Cuts at: 0 942 3468
Size: 942 2526
AflIII A'CryG_T
Cuts at: 0 3447 3468
Size: 3447 21
AleI CACnn'nnGTG
Cuts at: 0 2041 2416 3468
Size: 2041 375 1052
Fragments arranged by size:
2041 1052 375
AlwNI CAG_nnn'CTG
Cuts at: 0 974 1991 2805 3468
Size: 974 1017 814 663
Fragments arranged by size:
1017 974 814 663
ApaI G_GGCC'C
Cuts at: 0 459 470 2328 3468
Size: 459 11 1858 1140
Fragments arranged by size:
1858 1140 459 11
ApoI r'AATT_y
Cuts at: 0 19 1644 1803 1942 2317 3288 3394 3468
Size: 19 1625 159 139 375 971 106 74
Fragments arranged by size:
1625 971 375 159 139 106 74 19
AvaI C'yCGr_G
Cuts at: 0 28 86 224 315 1839 3468
Size: 28 58 138 91 1524 1629
Fragments arranged by size:
1629 1524 138 91 58 28
AvrII C'CTAG_G
Cuts at: 0 867 3468
Size: 867 2601
BamHI G'GATC_C
Cuts at: 0 344 492 2680 3468
Size: 344 148 2188 788
Fragments arranged by size:
2188 788 344 148
BanI G'GyrC_C
Cuts at: 0 32 67 577 1835 2262 3316 3468
Size: 32 35 510 1258 427 1054 152
Fragments arranged by size:
1258 1054 510 427 152 35 32
BanII G_rGCy'C
Cuts at: 0 114 459 470 694 1117 1795 2328 3468
Size: 114 345 11 224 423 678 533 1140
Fragments arranged by size:
1140 678 533 423 345 224 114 11
BbeI G_GCGC'C
Cuts at: 0 71 3468
Size: 71 3397
BbsI GAAGACnn'nnnn_
Cuts at: 0 292 3468
Size: 292 3176
BbvCI CC'TCA_GC
Cuts at: 0 1934 2601 3468
Size: 1934 667 867
Fragments arranged by size:
1934 867 667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 177 211 981 1015 3468
Size: 177 34 770 34 2453
Fragments arranged by size:
2453 770 177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1728 3468
Size: 1728 1740
BclI T'GATC_A
Cuts at: 0 1422 3468
Size: 1422 2046
BfrBI ATG'CAT
Cuts at: 0 2100 3468
Size: 2100 1368
BlpI GC'TnA_GC
Cuts at: 0 1064 2882 3468
Size: 1064 1818 586
Fragments arranged by size:
1818 1064 586
Bme1580I G_kGCm'C
Cuts at: 0 459 470 580 747 2265 2328 2367 2869
Size: 459 11 110 167 1518 63 39 502
Cuts at: 2869 3468
Size: 599
Fragments arranged by size:
1518 599 502 459 167 110 63 39
11
BmgBI CAC'GTC
Cuts at: 0 591 1652 3468
Size: 591 1061 1816
Fragments arranged by size:
1816 1061 591
BmrI ACTGGGnnnn_n'
Cuts at: 0 326 1979 2354 2966 3080 3468
Size: 326 1653 375 612 114 388
Fragments arranged by size:
1653 612 388 375 326 114
BmtI G_CTAG'C
Cuts at: 0 3221 3468
Size: 3221 247
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 962 3468
Size: 962 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 643 668 1767 2009 2384 2508 2993
Size: 457 186 25 1099 242 375 124 485
Cuts at: 2993 3468
Size: 475
Fragments arranged by size:
1099 485 475 457 375 242 186 124
25
Bpu10I CC'TnA_GC
Cuts at: 0 538 788 1777 1934 2002 2377 2601 3468
Size: 538 250 989 157 68 375 224 867
Fragments arranged by size:
989 867 538 375 250 224 157 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 436 1085 1153 1263 2637 3468
Size: 436 649 68 110 1374 831
Fragments arranged by size:
1374 831 649 436 110 68
BsaAI yAC'GTr
Cuts at: 0 391 3468
Size: 391 3077
BsaBI GATnn'nnATC
Cuts at: 0 491 3468
Size: 491 2977
BsaHI Gr'CG_yC
Cuts at: 0 68 92 679 3468
Size: 68 24 587 2789
Fragments arranged by size:
2789 587 68 24
BsaWI w'CCGG_w
Cuts at: 0 495 768 892 3468
Size: 495 273 124 2576
Fragments arranged by size:
2576 495 273 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 2094 2124 2469 2499 3468
Size: 580 30 1484 30 345 30 969
Fragments arranged by size:
1484 969 580 345 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 454 938 1373 1562 1565 2012 2099
Size: 341 113 484 435 189 3 447 87
Cuts at: 2099 2548 3468
Size: 449 920
Fragments arranged by size:
920 484 449 447 435 341 189 113
87 3
BseYI C'CCAG_C
Cuts at: 0 355 423 718 859 1184 2203 2763 3136
Size: 355 68 295 141 325 1019 560 373
Cuts at: 3136 3468
Size: 332
Fragments arranged by size:
1019 560 373 355 332 325 295 141
68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1234 1844 2231 2612 3188 3468
Size: 520 714 610 387 381 576 280
Fragments arranged by size:
714 610 576 520 387 381 280
BsiEI CG_ry'CG
Cuts at: 0 108 236 3468
Size: 108 128 3232
Fragments arranged by size:
3232 128 108
BsiHKAI G_wGCw'C
Cuts at: 0 694 1795 3468
Size: 694 1101 1673
Fragments arranged by size:
1673 1101 694
BsmI GAATG_Cn'
Cuts at: 0 1363 3468
Size: 1363 2105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 581 686 3468
Size: 581 105 2782
Fragments arranged by size:
2782 581 105
Bsp1286I G_dGCh'C
Cuts at: 0 114 459 470 580 694 747 1117 1795
Size: 114 345 11 110 114 53 370 678
Cuts at: 1795 2265 2328 2367 2869 3468
Size: 470 63 39 502 599
Fragments arranged by size:
678 599 502 470 370 345 114 114
110 63 53 39 11
BspEI T'CCGG_A
Cuts at: 0 768 892 3468
Size: 768 124 2576
Fragments arranged by size:
2576 768 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1068 3468
Size: 1068 2400
BsrBI CCG'CTC
Cuts at: 0 76 3468
Size: 76 3392
BsrDI GCAATG_nn'
Cuts at: 0 997 1027 1815 2594 3468
Size: 997 30 788 779 874
Fragments arranged by size:
997 874 788 779 30
BsrFI r'CCGG_y
Cuts at: 0 41 255 396 3468
Size: 41 214 141 3072
Fragments arranged by size:
3072 214 141 41
BstXI CCAn_nnnn'nTGG
Cuts at: 0 435 1034 1915 3421 3468
Size: 435 599 881 1506 47
Fragments arranged by size:
1506 881 599 435 47
BstYI r'GATC_y
Cuts at: 0 344 492 2680 3322 3468
Size: 344 148 2188 642 146
Fragments arranged by size:
2188 642 344 148 146
BtgI C'CryG_G
Cuts at: 0 141 3468
Size: 141 3327
BtsI GCAGTG_nn'
Cuts at: 0 1021 1827 2213 2430 3303 3468
Size: 1021 806 386 217 873 165
Fragments arranged by size:
1021 873 806 386 217 165
DraI TTT'AAA
Cuts at: 0 2291 2672 3468
Size: 2291 381 796
Fragments arranged by size:
2291 796 381
DraIII CAC_nnn'GTG
Cuts at: 0 391 2809 3468
Size: 391 2418 659
Fragments arranged by size:
2418 659 391
EaeI y'GGCC_r
Cuts at: 0 233 275 3186 3468
Size: 233 42 2911 282
Fragments arranged by size:
2911 282 233 42
EagI C'GGCC_G
Cuts at: 0 233 3468
Size: 233 3235
EarI CTCTTCn'nnn_
Cuts at: 0 468 842 1105 1397 1598 3468
Size: 468 374 263 292 201 1870
Fragments arranged by size:
1870 468 374 292 263 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 3468
Size: 142 3326
EcoICRI GAG'CTC
Cuts at: 0 692 1793 3468
Size: 692 1101 1675
Fragments arranged by size:
1675 1101 692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 457 585 643 668 1739 1767 2009
Size: 161 296 128 58 25 1071 28 242
Cuts at: 2009 2384 2508 2777 2993 3468
Size: 375 124 269 216 475
Fragments arranged by size:
1071 475 375 296 269 242 216 161
128 124 58 28 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 978 3468
Size: 978 2490
EcoO109I rG'GnC_Cy
Cuts at: 0 249 466 530 569 706 971 1616 2324
Size: 249 217 64 39 137 265 645 708
Cuts at: 2324 2325 3028 3082 3177 3337 3468
Size: 1 703 54 95 160 131
Fragments arranged by size:
708 703 645 265 249 217 160 137
131 95 64 54 39 1
EcoRI G'AATT_C
Cuts at: 0 1644 3288 3468
Size: 1644 1644 180
Fragments arranged by size:
1644 1644 180
HaeII r_GCGC'y
Cuts at: 0 71 784 944 1474 3468
Size: 71 713 160 530 1994
Fragments arranged by size:
1994 713 530 160 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 198 230 336 368 580 612 1295 1327
Size: 198 32 106 32 212 32 683 32
Cuts at: 1327 2830 2862 3468
Size: 1503 32 606
Fragments arranged by size:
1503 683 606 212 198 106 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2843 3468
Size: 2843 625
HindIII A'AGCT_T
Cuts at: 0 1973 3468
Size: 1973 1495
HpaI GTT'AAC
Cuts at: 0 2843 3468
Size: 2843 625
KasI G'GCGC_C
Cuts at: 0 67 3468
Size: 67 3401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2303 2973 3312 3468
Size: 2303 670 339 156
Fragments arranged by size:
2303 670 339 156
MscI TGG'CCA
Cuts at: 0 277 3188 3468
Size: 277 2911 280
Fragments arranged by size:
2911 280 277
MslI CAynn'nnrTG
Cuts at: 0 1032 1820 2041 2416 2959 3419 3468
Size: 1032 788 221 375 543 460 49
Fragments arranged by size:
1032 788 543 460 375 221 49
MspA1I CmG'CkG
Cuts at: 0 143 545 885 1011 2207 3468
Size: 143 402 340 126 1196 1261
Fragments arranged by size:
1261 1196 402 340 143 126
NaeI GCC'GGC
Cuts at: 0 43 257 398 3468
Size: 43 214 141 3070
Fragments arranged by size:
3070 214 141 43
NarI GG'CG_CC
Cuts at: 0 68 3468
Size: 68 3400
NdeI CA'TA_TG
Cuts at: 0 3435 3468
Size: 3435 33
NgoMIV G'CCGG_C
Cuts at: 0 41 255 396 3468
Size: 41 214 141 3072
Fragments arranged by size:
3072 214 141 41
NheI G'CTAG_C
Cuts at: 0 3217 3468
Size: 3217 251
NotI GC'GGCC_GC
Cuts at: 0 233 3468
Size: 233 3235
NsiI A_TGCA'T
Cuts at: 0 2102 3468
Size: 2102 1366
NspI r_CATG'y
Cuts at: 0 366 3072 3451 3468
Size: 366 2706 379 17
Fragments arranged by size:
2706 379 366 17
PasI CC'CwG_GG
Cuts at: 0 573 582 613 3468
Size: 573 9 31 2855
Fragments arranged by size:
2855 573 31 9
PciI A'CATG_T
Cuts at: 0 3447 3468
Size: 3447 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 64 799 2809 3195 3468
Size: 64 735 2010 386 273
Fragments arranged by size:
2010 735 386 273 64
PfoI T'CCnGG_A
Cuts at: 0 434 2295 2524 3468
Size: 434 1861 229 944
Fragments arranged by size:
1861 944 434 229
PmlI CAC'GTG
Cuts at: 0 391 3468
Size: 391 3077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2038 2070 3468
Size: 2038 32 1398
Fragments arranged by size:
2038 1398 32
PpuMI rG'GwC_Cy
Cuts at: 0 249 530 569 1616 3028 3177 3468
Size: 249 281 39 1047 1412 149 291
Fragments arranged by size:
1412 1047 291 281 249 149 39
PshAI GACnn'nnGTC
Cuts at: 0 1852 3468
Size: 1852 1616
PsiI TTA'TAA
Cuts at: 0 1451 3468
Size: 1451 2017
PspOMI G'GGCC_C
Cuts at: 0 455 466 2324 3468
Size: 455 11 1858 1144
Fragments arranged by size:
1858 1144 455 11
PstI C_TGCA'G
Cuts at: 0 818 2436 3374 3468
Size: 818 1618 938 94
Fragments arranged by size:
1618 938 818 94
SacI G_AGCT'C
Cuts at: 0 694 1795 3468
Size: 694 1101 1673
Fragments arranged by size:
1673 1101 694
SacII CC_GC'GG
Cuts at: 0 144 3468
Size: 144 3324
SanDI GG'GwC_CC
Cuts at: 0 3177 3468
Size: 3177 291
SapI GCTCTTCn'nnn_
Cuts at: 0 842 1105 1397 3468
Size: 842 263 292 2071
Fragments arranged by size:
2071 842 292 263
SexAI A'CCwGG_T
Cuts at: 0 2826 3468
Size: 2826 642
SfcI C'TryA_G
Cuts at: 0 814 2057 2270 2432 3370 3468
Size: 814 1243 213 162 938 98
Fragments arranged by size:
1243 938 814 213 162 98
SfoI GGC'GCC
Cuts at: 0 69 3468
Size: 69 3399
SmaI CCC'GGG
Cuts at: 0 30 88 1841 3468
Size: 30 58 1753 1627
Fragments arranged by size:
1753 1627 58 30
StuI AGG'CCT
Cuts at: 0 174 3468
Size: 174 3294
StyI C'CwwG_G
Cuts at: 0 867 934 1103 2117 2226 3468
Size: 867 67 169 1014 109 1242
Fragments arranged by size:
1242 1014 867 169 109 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 306 460 595 1198 1408 3150 3427 3468
Size: 306 154 135 603 210 1742 277 41
Fragments arranged by size:
1742 603 306 277 210 154 135 41
TatI w'GTAC_w
Cuts at: 0 2537 3468
Size: 2537 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 319 351 2607 2639 3468
Size: 319 32 2256 32 829
Fragments arranged by size:
2256 829 319 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1281 1344 2806 3468
Size: 1281 63 1462 662
Fragments arranged by size:
1462 1281 662 63
XmaI C'CCGG_G
Cuts at: 0 28 86 1839 3468
Size: 28 58 1753 1629
Fragments arranged by size:
1753 1629 58 28
XmnI GAAnn'nnTTC
Cuts at: 0 919 3468
Size: 919 2549
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BstXI BstYI BtgI BtsI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HindIII HpaI KasI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI SacI SacII SanDI SapI
SexAI SfcI SfoI SmaI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI BaeI BglI BglII BpuEI
BsiWI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EcoRV FalI
FseI FspI FspAI KpnI MfeI MluI NcoI NruI
PacI PmeI PspXI PsrI PvuI PvuII RsrII SalI
SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI