################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 189 CCGC CCGC(-3/-1) AcyI 316 GGCGTC G[GA]^CG[CT]C BanI 324 GGCACC G^G[CT][GA]CC BanII 370 GGGCTC G[GA]GC[CT]^C BciVI 397 GTATCC GTATCC(6/5) BfaI 382 CTAG C^TAG BmyI 370 GGGCTC G[AGT]GC[ACT]^C BsaHI 316 GGCGTC G[GA]^CG[CT]C BsaI 83 GAGACC GGTCTC(1/5) BsaWI 374 TCCGGT [AT]^CCGG[AT] BsmAI 83 GAGAC GTCTC(1/5) Bsp1286I 370 GGGCTC G[AGT]GC[ACT]^C BstF5I 351 CATCC GGATG(2/0) BstYI 161 GGATCT [GA]^GATC[CT] Cac8I 119 GCAAGC GC[ACGT]^[ACGT]GC DdeI 278 CTGAG C^T[ACGT]AG DsaI 56 CCATGG C^C[GA][CT]GG EarI 411 GAAGAG CTCTTC(1/4) EcoT14I 56 CCATGG C^C[AT][AT]GG FokI 351 CATCC GGATG(9/13) HaeIII 139 GGCC GG^CC HapII 375 CCGG C^CGG Hin1I 316 GGCGTC G[GA]^CG[CT]C HpaII 375 CCGG C^CGG Ksp632I 411 GAAGAG CTCTTC(1/4) MaeI 382 CTAG C^TAG MflI 161 GGATCT [GA]^GATC[CT] MseI 22 TTAA T^TAA MslI 51 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 189 CCGCTG C[AC]G^C[GT]G MspI 375 CCGG C^CGG MwoI 363 GCTGGTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 56 CCATGG C^CATGG NlaIII 57 CATG CATG^ StyI 56 CCATGG C^C[AT][AT]GG Tru9I 22 TTAA T^TAA XbaI 381 TCTAGA T^CTAGA XcmI 47 CCATCATAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 161 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 186 GATCC GGATC(4/5) 161 GGATC AvaII 152 GGACC G^G[AT]CC 245 GGACC BsrI 115 ACTGG ACTGG(1/-1) 394 CCAGT Cfr13I 152 GGACC G^G[ACGT]CC 245 GGACC DpnII 162 GATC ^GATC 186 GATC MboI 162 GATC ^GATC 186 GATC MboII 408 GAAGA GAAGA(8/7) 411 GAAGA NdeII 162 GATC ^GATC 186 GATC NlaIV 324 GGCACC GG[ACGT]^[ACGT]CC 371 GGCTCC PstI 70 CTGCAG CTGCA^G 299 CTGCAG Sau3AI 162 GATC ^GATC 186 GATC Sau96I 152 GGACC G^G[ACGT]CC 245 GGACC SfcI 70 CTGCAG C^T[GA][CT]AG 299 CTGCAG TspRI 114 CACTG CA[GC]TG[ACGT][ACGT]^ 182 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BssKI BstNI EcoRII Fnu4HI HgaI ItaI MnlI MvaI ScrFI TseI