(Linear) (Six-base) MAPSORT of: KIEE1301.seq  Check: 9277  from: 1  to: 420



KIEE1301.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:35  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     288     320     420 
   Size:        288      32     100

  Fragments arranged by size: 

                 288     100      32
 
BanI G'GyrC_C

Cuts at:      0     324     420 
   Size:        324      96
 
BanII G_rGCy'C

Cuts at:      0     374     420 
   Size:        374      46
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     408     420 
   Size:        408      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      77     420 
   Size:         77     343
 
BsaHI Gr'CG_yC

Cuts at:      0     317     420 
   Size:        317     103
 
BsaWI w'CCGG_w

Cuts at:      0     374     420 
   Size:        374      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     288     318     420 
   Size:        288      30     102

  Fragments arranged by size: 

                 288     102      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     374     420 
   Size:        374      46
 
BstYI r'GATC_y

Cuts at:      0     161     420 
   Size:        161     259
 
BtgI C'CryG_G

Cuts at:      0      56     420 
   Size:         56     364
 
EarI CTCTTCn'nnn_

Cuts at:      0     406     420 
   Size:        406      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     213     245     420 
   Size:        213      32     175

  Fragments arranged by size: 

                 213     175      32
 
MslI CAynn'nnrTG

Cuts at:      0      55     420 
   Size:         55     365
 
MspA1I CmG'CkG

Cuts at:      0     191     420 
   Size:        191     229
 
NcoI C'CATG_G

Cuts at:      0      56     420 
   Size:         56     364
 
PasI CC'CwG_GG

Cuts at:      0     261     420 
   Size:        261     159
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     288     320     420 
   Size:        288      32     100

  Fragments arranged by size: 

                 288     100      32
 
PstI C_TGCA'G

Cuts at:      0      74     303     420 
   Size:         74     229     117

  Fragments arranged by size: 

                 229     117      74
 
SfcI C'TryA_G

Cuts at:      0      70     299     420 
   Size:         70     229     121

  Fragments arranged by size: 

                 229     121      70
 
StyI C'CwwG_G

Cuts at:      0      56     420 
   Size:         56     364
 
XbaI T'CTAG_A

Cuts at:      0     381     420 
   Size:        381      39
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      54     420 
   Size:         54     366
 

 Enzymes that do cut:

     AloI     BanI    BanII    BciVI     BsaI    BsaHI    BsaWI    BsaXI
 Bsp1286I    BstYI     BtgI     EarI    Hin4I     MslI   MspA1I     NcoI
     PasI     PpiI     PstI     SfcI     StyI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XhoI     XmaI     XmnI     ZraI