Restriction map for
pFN21AE0210
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NlaIV NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 637 CTTAAG C^TTAAG
Aor51HI 294 AGCGCT AGC^GCT
AvaII 375 GGACC G^G[AT]CC
BalI 317 TGGCCA TGG^CCA
BbvCI 306 GCTGAGG CCTCAGC(-5/-2)
BcnI 358 CCCGG CC^[GC]GG
BfrI 637 CTTAAG C^TTAAG
BglII 667 AGATCT A^GATCT
BmyI 339 GTGCCC G[AGT]GC[ACT]^C
BpmI 165 CTCCAG CTGGAG(16/14)
BseRI 362 GAGGAG GAGGAG(10/8)
BsmBI 367 GAGACG CGTCTC(1/5)
Bsp1286I 339 GTGCCC G[AGT]GC[ACT]^C
BspHI 176 TCATGA T^CATGA
BstF5I 701 GGATG GGATG(2/0)
Cac8I 4 GCTCGC GC[ACGT]^[ACGT]GC
CfoI 295 GCGC GCG^C
DraI 106 TTTAAA TTT^AAA
DsaI 314 CCATGG C^C[GA][CT]GG
EaeI 317 TGGCCA [CT]^GGCC[GA]
Eco47III 294 AGCGCT AGC^GCT
EcoT22I 196 ATGCAT ATGCA^T
FokI 701 GGATG GGATG(9/13)
HaeII 294 AGCGCT [GA]GCGC^[CT]
HgaI 369 GACGC GACGC(5/10)
HhaI 295 GCGC GCG^C
HinP1I 295 GCGC G^CGC
HincII 419 GTCAAC GT[CT]^[GA]AC
HindII 419 GTCAAC GT[CT]^[GA]AC
HphI 381 GGTGA GGTGA(8/7)
MluNI 317 TGGCCA TGG^CCA
MscI 317 TGGCCA TGG^CCA
MslI 784 CATCTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 358 CCCGG CC^[GC]GG
NcoI 314 CCATGG C^CATGG
NsiI 196 ATGCAT ATGCA^T
PshAI 18 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 119 CAGCTG CAG^CTG
RcaI 176 TCATGA T^CATGA
SexAI 377 ACCAGGT A^CC[AT]GGT
SfcI 775 CTACAG C^T[GA][CT]AG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BsmAI 367 GAGAC GTCTC(1/5)
707 GTCTC
BstYI 466 GGATCT [GA]^GATC[CT]
667 AGATCT
Cfr13I 311 GGGCC G^G[ACGT]CC
375 GGACC
EarI 334 GAAGAG CTCTTC(1/4)
734 GAAGAG
HaeIII 312 GGCC GG^CC
318 GGCC
HapII 27 CCGG C^CGG
359 CCGG
HpaII 27 CCGG C^CGG
359 CCGG
Ksp632I 334 GAAGAG CTCTTC(1/4)
734 GAAGAG
MaeIII 590 GTCAC ^GT[ACGT]AC
685 GTCAC
MboII 334 GAAGA GAAGA(8/7)
734 GAAGA
MflI 466 GGATCT [GA]^GATC[CT]
667 AGATCT
MspA1I 119 CAGCTG C[AC]G^C[GT]G
549 CAGCGG
MspI 27 CCGG C^CGG
359 CCGG
Sau96I 311 GGGCC G^G[ACGT]CC
375 GGACC
SmlI 252 CTCAAG C^T[CT][GA]AG
637 CTTAAG
Tsp45I 590 GTCAC ^GT[GC]AC
685 GTCAC
XhoII 466 GGATCT [GA]^GATC[CT]
667 AGATCT
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI BbvI BsaJI BsiYI BslI
BsrI BssKI BstAPI BstNI DdeI
DpnII EcoRII EcoT14I Fnu4HI HinfI
ItaI MboI MnlI MseI MvaI
MwoI NdeII NlaIII PleI Sau3AI
ScrFI StyI Tru9I TseI Tsp509I
TspRI