(Circular) (Six-base) MAPSORT of: pFN21AE0402.seq Check: 3100 from: 1 to: 5617 pFN21AE0402 5617 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3427 278 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2757 2960 1937 Size: 820 203 4594 Fragments arranged by size: 4594 820 203 Acc65I G'GTAC_C Cuts at: 2938 2938 Size: 5617 AclI AA'CG_TT Cuts at: 1563 3744 4117 1563 Size: 2181 373 3063 Fragments arranged by size: 3063 2181 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 2862 2896 3680 5064 5332 1632 Size: 689 541 34 784 1384 268 1917 Fragments arranged by size: 1917 1384 784 689 541 268 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5420 AflIII A'CryG_T Cuts at: 1203 2544 4516 5493 1203 Size: 1341 1972 977 1327 Fragments arranged by size: 1972 1341 1327 977 AgeI A'CCGG_T Cuts at: 4448 4448 Size: 5617 AhdI GACnn_n'nnGTC Cuts at: 4346 4346 Size: 5617 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5617 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5617 AlwNI CAG_nnn'CTG Cuts at: 2228 3360 4932 2228 Size: 1132 1572 2913 Fragments arranged by size: 2913 1572 1132 ApaLI G'TGCA_C Cuts at: 2597 3674 4830 2597 Size: 1077 1156 3384 Fragments arranged by size: 3384 1156 1077 ApoI r'AATT_y Cuts at: 1493 2464 2807 2926 3152 3304 5413 1493 Size: 971 343 119 226 152 2109 1697 Fragments arranged by size: 2109 1697 971 343 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 4171 160 Size: 872 3139 1606 Fragments arranged by size: 3139 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 AvaI C'yCGr_G Cuts at: 1870 1943 2942 1870 Size: 73 999 4545 Fragments arranged by size: 4545 999 73 BamHI G'GATC_C Cuts at: 2769 2947 3314 2769 Size: 178 367 5072 Fragments arranged by size: 5072 367 178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2938 4393 618 Size: 325 217 627 1151 1455 1842 Fragments arranged by size: 1842 1455 1151 627 325 217 BanII G_rGCy'C Cuts at: 1391 2179 2936 1391 Size: 788 757 4072 Fragments arranged by size: 4072 788 757 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2066 928 Size: 971 167 4479 Fragments arranged by size: 4479 971 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 1903 Size: 34 1871 34 3678 Fragments arranged by size: 3678 1871 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3509 4719 3509 Size: 1210 4407 BclI T'GATC_A Cuts at: 1548 1548 Size: 5617 BfrBI ATG'CAT Cuts at: 2348 2348 Size: 5617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4228 136 Size: 107 122 71 631 605 2556 1525 Fragments arranged by size: 2556 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5612 5612 Size: 5617 BlpI GC'TnA_GC Cuts at: 3034 3034 Size: 5617 Bme1580I G_kGCm'C Cuts at: 1790 2601 3678 4834 1790 Size: 811 1077 1156 2573 Fragments arranged by size: 2573 1156 1077 811 BmgBI CAC'GTC Cuts at: 1318 2366 1318 Size: 1048 4569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3348 4306 442 Size: 416 53 83 399 24 1931 958 1753 Fragments arranged by size: 1931 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5617 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2578 2578 Size: 5617 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2910 4277 1239 Size: 1671 1367 2579 Fragments arranged by size: 2579 1671 1367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3742 4607 4905 3742 Size: 865 298 4454 Fragments arranged by size: 4454 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4280 882 Size: 617 2781 2219 Fragments arranged by size: 2781 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2547 3408 493 Size: 711 1343 861 2702 Fragments arranged by size: 2702 1343 861 711 BsaBI GATnn'nnATC Cuts at: 5611 5611 Size: 5617 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3424 3806 4441 275 Size: 53 83 186 936 1891 382 635 1451 Fragments arranged by size: 1891 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4050 4448 4722 4869 5280 4050 Size: 398 274 147 411 4387 Fragments arranged by size: 4387 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5392 Fragments arranged by size: 5392 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5617 BseYI C'CCAG_C Cuts at: 2326 4820 5316 2326 Size: 2494 496 2627 Fragments arranged by size: 2627 2494 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5044 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3067 3828 4856 1540 Size: 275 186 1066 761 1028 2301 Fragments arranged by size: 2301 1066 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2445 2601 3678 3763 4834 2445 Size: 156 1077 85 1071 3228 Fragments arranged by size: 3228 1077 1071 156 85 BsmI GAATG_Cn' Cuts at: 2348 2855 3128 3221 2348 Size: 507 273 93 4744 Fragments arranged by size: 4744 507 273 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2445 2601 2936 3678 3763 4834 Size: 399 389 266 156 335 742 85 1071 Cuts at: 4834 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 389 335 266 156 85 BspEI T'CCGG_A Cuts at: 5280 5280 Size: 5617 BspHI T'CATG_A Cuts at: 3506 5236 3506 Size: 1730 3887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2972 844 Size: 2128 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3504 1394 Size: 429 1681 3507 Fragments arranged by size: 3507 1681 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4112 4286 65 Size: 1183 2864 174 1396 Fragments arranged by size: 2864 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4261 4448 1700 Size: 110 2451 187 2869 Fragments arranged by size: 2869 2451 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5617 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5617 BssSI C'ACGA_G Cuts at: 3677 4689 3677 Size: 1012 4605 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5617 BstBI TT'CG_AA Cuts at: 4421 4421 Size: 5617 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5617 BstYI r'GATC_y Cuts at: 1972 2769 2947 3314 3699 3716 5168 5254 5266 Size: 797 178 367 385 17 1452 86 12 Cuts at: 5266 5612 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 797 385 367 346 178 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2688 513 Size: 552 939 684 3442 Fragments arranged by size: 3442 939 684 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4878 Fragments arranged by size: 4878 683 56 BtsI GCAGTG_nn' Cuts at: 2026 3133 3927 3947 2026 Size: 1107 794 20 3696 Fragments arranged by size: 3696 1107 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5582 DraI TTT'AAA Cuts at: 2830 2921 2830 Size: 91 5526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2753 4624 809 Size: 507 1437 1871 1802 Fragments arranged by size: 1871 1802 1437 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3064 3953 5582 Size: 54 1425 50 275 18 1234 889 1629 Cuts at: 5582 8 Size: 43 Fragments arranged by size: 1629 1425 1234 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3064 1537 Size: 275 1252 4090 Fragments arranged by size: 4090 1252 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 2160 2252 2655 3547 1353 Size: 675 132 92 403 892 3423 Fragments arranged by size: 3423 892 675 403 132 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4204 4578 4724 387 Size: 1064 138 2615 374 146 1280 Fragments arranged by size: 2615 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2862 2896 2910 3680 4277 5064 Size: 393 689 541 34 14 770 597 787 Cuts at: 5064 5332 1239 Size: 268 1524 Fragments arranged by size: 1524 787 770 689 597 541 393 268 34 14 EcoNI CCTnn'n_nnAGG Cuts at: 2184 2184 Size: 5617 EcoO109I rG'GnC_Cy Cuts at: 2190 2359 2190 Size: 169 5448 EcoRI G'AATT_C Cuts at: 2464 2807 2926 3304 2464 Size: 343 119 378 4777 Fragments arranged by size: 4777 378 343 119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 5617 FspI TGC'GCA Cuts at: 4123 4123 Size: 5617 HaeII r_GCGC'y Cuts at: 1678 4764 1678 Size: 3086 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 4258 4290 4332 Size: 32 135 32 542 32 2112 32 42 Cuts at: 4332 4364 1373 Size: 32 2626 Fragments arranged by size: 2626 2112 542 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2073 2457 2961 3207 669 Size: 988 281 135 384 504 246 3079 Fragments arranged by size: 3079 988 504 384 281 246 135 HindIII A'AGCT_T Cuts at: 748 748 Size: 5617 HpaI GTT'AAC Cuts at: 2457 3207 2457 Size: 750 4867 KpnI G_GTAC'C Cuts at: 2942 2942 Size: 5617 MfeI C'AATT_G Cuts at: 3216 3216 Size: 5617 MluI A'CGCG_T Cuts at: 5493 5493 Size: 5617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2747 4731 4915 1305 Size: 1442 1984 184 2007 Fragments arranged by size: 2007 1984 1442 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3795 Fragments arranged by size: 3795 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2633 2726 3575 3934 4093 518 Size: 648 1467 93 849 359 159 2042 Fragments arranged by size: 2042 1467 849 648 359 159 93 MspA1I CmG'CkG Cuts at: 1606 2330 3033 3710 4858 5103 1606 Size: 724 703 677 1148 245 2120 Fragments arranged by size: 2120 1148 724 703 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4126 Fragments arranged by size: 4126 939 552 NdeI CA'TA_TG Cuts at: 387 2493 387 Size: 2106 3511 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5617 NotI GC'GGCC_GC Cuts at: 3064 3064 Size: 5617 NsiI A_TGCA'T Cuts at: 2350 2350 Size: 5617 NspI r_CATG'y Cuts at: 1132 1229 2975 4520 1132 Size: 97 1746 1545 2229 Fragments arranged by size: 2229 1746 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5617 PciI A'CATG_T Cuts at: 4516 4516 Size: 5617 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5617 PmeI GTTT'AAAC Cuts at: 2921 2921 Size: 5617 PmlI CAC'GTG Cuts at: 2547 2547 Size: 5617 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3686 3718 5199 5231 3686 Size: 32 1481 32 4072 Fragments arranged by size: 4072 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2359 Size: 5617 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5617 PsiI TTA'TAA Cuts at: 3187 3187 Size: 5617 PstI C_TGCA'G Cuts at: 830 2206 2969 830 Size: 1376 763 3478 Fragments arranged by size: 3478 1376 763 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5617 PvuII CAG'CTG Cuts at: 2330 2330 Size: 5617 SalI G'TCGA_C Cuts at: 1936 2959 1936 Size: 1023 4594 SapI GCTCTTCn'nnn_ Cuts at: 2160 2655 2160 Size: 495 5122 SbfI CC_TGCA'GG Cuts at: 2969 2969 Size: 5617 ScaI AGT'ACT Cuts at: 3865 5577 3865 Size: 1712 3905 SfcI C'TryA_G Cuts at: 826 1045 2202 2965 4100 4781 4972 826 Size: 219 1157 763 1135 681 191 1471 Fragments arranged by size: 1471 1157 1135 763 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5617 SmaI CCC'GGG Cuts at: 1872 2944 1872 Size: 1072 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3721 4622 4884 820 Size: 197 926 1778 901 262 1553 Fragments arranged by size: 1778 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3408 493 Size: 2915 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5617 SphI G_CATG'C Cuts at: 1132 2975 1132 Size: 1843 3774 SspI AAT'ATT Cuts at: 5 52 3541 5 Size: 47 3489 2081 Fragments arranged by size: 3489 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5617 StyI C'CwwG_G Cuts at: 513 1065 2004 2389 3056 513 Size: 552 939 385 667 3074 Fragments arranged by size: 3074 939 667 552 385 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3644 3661 3814 3999 4444 325 Size: 1255 2064 17 153 185 445 1498 Fragments arranged by size: 2064 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 2718 3863 Size: 274 80 33 51 1443 276 465 1145 Cuts at: 3863 5575 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1145 465 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5577 5609 5577 Size: 32 5585 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5617 XbaI T'CTAG_A Cuts at: 2743 2953 2743 Size: 210 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5617 XmaI C'CCGG_G Cuts at: 1870 2942 1870 Size: 1072 4545 XmnI GAAnn'nnTTC Cuts at: 2306 2855 3746 2306 Size: 549 891 4177 Fragments arranged by size: 4177 891 549 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3425 276 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI