(Circular) (Six-base) MAPSORT of: pFN21AE0404.seq Check: 8189 from: 1 to: 5527 pFN21AE0404 5527 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2534 3337 278 Size: 53 83 186 936 998 803 2468 Fragments arranged by size: 2468 998 936 803 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2848 2848 Size: 5527 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3590 4974 5242 1632 Size: 1958 1384 268 1917 Fragments arranged by size: 1958 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5330 AflIII A'CryG_T Cuts at: 1203 2059 4426 5403 1203 Size: 856 2367 977 1327 Fragments arranged by size: 2367 1327 977 856 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 2494 4256 2494 Size: 1762 3765 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5527 AlwNI CAG_nnn'CTG Cuts at: 2620 2701 3270 4842 2620 Size: 81 569 1572 3305 Fragments arranged by size: 3305 1572 569 81 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2195 2456 2836 3062 3214 5323 1493 Size: 702 261 380 226 152 2109 1697 Fragments arranged by size: 2109 1697 702 380 261 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2852 1870 Size: 73 909 4545 Fragments arranged by size: 4545 909 73 BamHI G'GATC_C Cuts at: 2857 3224 2857 Size: 367 5160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2848 4303 618 Size: 325 217 627 1061 1455 1842 Fragments arranged by size: 1842 1455 1061 627 325 217 BanII G_rGCy'C Cuts at: 1391 2352 2846 1391 Size: 961 494 4072 Fragments arranged by size: 4072 961 494 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4556 BbvCI CC'TCA_GC Cuts at: 2684 2684 Size: 5527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3718 3752 1903 Size: 34 50 34 1697 34 3678 Fragments arranged by size: 3678 1697 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3419 4629 3419 Size: 1210 4317 BclI T'GATC_A Cuts at: 1548 1548 Size: 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4138 136 Size: 107 122 71 631 605 2466 1525 Fragments arranged by size: 2466 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5522 5522 Size: 5527 BlpI GC'TnA_GC Cuts at: 2052 2944 2052 Size: 892 4635 Bme1580I G_kGCm'C Cuts at: 1790 3588 4744 1790 Size: 1798 1156 2573 Fragments arranged by size: 2573 1798 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5527 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3258 4216 442 Size: 416 53 83 399 24 1841 958 1753 Fragments arranged by size: 1841 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 2366 2606 4187 1239 Size: 1033 94 240 1581 2579 Fragments arranged by size: 2579 1581 1033 240 94 Bpu10I CC'TnA_GC Cuts at: 2684 2684 Size: 5527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3652 4517 4815 3652 Size: 865 298 4364 Fragments arranged by size: 4364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3318 493 Size: 711 2114 2702 Fragments arranged by size: 2702 2114 711 BsaBI GATnn'nnATC Cuts at: 5521 5521 Size: 5527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2531 2600 3334 3716 Size: 53 83 186 936 998 69 734 382 Cuts at: 3716 4351 275 Size: 635 1451 Fragments arranged by size: 1451 998 936 734 635 382 186 83 69 53 BsaWI w'CCGG_w Cuts at: 2014 2032 3960 4358 4632 4779 5190 2014 Size: 18 1928 398 274 147 411 2351 Fragments arranged by size: 2351 1928 411 398 274 147 18 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5302 Fragments arranged by size: 5302 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5527 BseYI C'CCAG_C Cuts at: 2662 2797 4730 5226 2662 Size: 135 1933 496 2963 Fragments arranged by size: 2963 1933 496 135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4954 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2977 3738 4766 1540 Size: 275 186 976 761 1028 2301 Fragments arranged by size: 2301 1028 976 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2352 2379 3588 3673 4744 2352 Size: 27 1209 85 1071 3135 Fragments arranged by size: 3135 1209 1071 85 27 BsmI GAATG_Cn' Cuts at: 3038 3131 3038 Size: 93 5434 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2379 2846 3588 3673 4744 1391 Size: 399 562 27 467 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 467 399 85 27 BspEI T'CCGG_A Cuts at: 2014 5190 2014 Size: 3176 2351 BspHI T'CATG_A Cuts at: 3416 5146 3416 Size: 1730 3797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 2569 2882 844 Size: 1655 70 313 3489 Fragments arranged by size: 3489 1655 313 70 BsrBI CCG'CTC Cuts at: 1394 1823 3414 1394 Size: 429 1591 3507 Fragments arranged by size: 3507 1591 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2695 2808 4022 4196 65 Size: 1183 1447 113 1214 174 1396 Fragments arranged by size: 1447 1396 1214 1183 174 113 BsrFI r'CCGG_y Cuts at: 1700 1810 2576 4171 4358 1700 Size: 110 766 1595 187 2869 Fragments arranged by size: 2869 1595 766 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5527 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5527 BssSI C'ACGA_G Cuts at: 3587 4599 3587 Size: 1012 4515 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5527 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2436 2782 2857 3224 3609 3626 5078 5164 Size: 464 346 75 367 385 17 1452 86 Cuts at: 5164 5176 5522 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 464 385 367 346 346 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 2391 2740 513 Size: 552 1326 349 3300 Fragments arranged by size: 3300 1326 552 349 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 2091 3043 3837 3857 2091 Size: 952 794 20 3761 Fragments arranged by size: 3761 952 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5492 DraI TTT'AAA Cuts at: 2831 2831 Size: 5527 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2974 3863 5492 Size: 54 1425 50 275 18 1144 889 1629 Cuts at: 5492 8 Size: 43 Fragments arranged by size: 1629 1425 1144 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2974 1537 Size: 275 1162 4090 Fragments arranged by size: 4090 1162 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 3457 1353 Size: 1452 652 3423 Fragments arranged by size: 3423 1452 652 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 4488 4634 387 Size: 1064 138 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2350 2350 Size: 5527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2366 2606 3590 4187 4974 5242 Size: 393 640 94 240 984 597 787 268 Cuts at: 5242 1239 Size: 1524 Fragments arranged by size: 1524 984 787 640 597 393 268 240 94 EcoO109I rG'GnC_Cy Cuts at: 2488 2680 2488 Size: 192 5335 EcoRI G'AATT_C Cuts at: 2195 2456 2836 3214 2195 Size: 261 380 378 4508 Fragments arranged by size: 4508 380 378 261 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 2023 4674 1678 Size: 345 2651 2531 Fragments arranged by size: 2651 2531 345 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2049 2081 2480 2512 4168 Size: 32 135 32 477 32 399 32 1656 Cuts at: 4168 4200 4242 4274 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1656 477 399 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2871 3117 669 Size: 988 281 933 246 3079 Fragments arranged by size: 3079 988 933 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KpnI G_GTAC'C Cuts at: 2852 2852 Size: 5527 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4641 4825 1305 Size: 3336 184 2007 Fragments arranged by size: 3336 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3705 Fragments arranged by size: 3705 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 3485 3844 4003 518 Size: 648 906 1413 359 159 2042 Fragments arranged by size: 2042 1413 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2344 2652 2943 3620 4768 5013 1606 Size: 489 249 308 291 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 489 308 291 249 245 NaeI GCC'GGC Cuts at: 2578 2578 Size: 5527 NcoI C'CATG_G Cuts at: 513 1065 2391 513 Size: 552 1326 3649 Fragments arranged by size: 3649 1326 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5527 NgoMIV G'CCGG_C Cuts at: 2576 2576 Size: 5527 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2063 2885 4430 1132 Size: 97 834 822 1545 2229 Fragments arranged by size: 2229 1545 834 822 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5527 PciI A'CATG_T Cuts at: 2059 4426 2059 Size: 2367 3160 PflMI CCAn_nnn'nTGG Cuts at: 1108 2620 1108 Size: 1512 4015 PfoI T'CCnGG_A Cuts at: 2460 2460 Size: 5527 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2488 Size: 5527 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5527 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PstI C_TGCA'G Cuts at: 830 2879 830 Size: 2049 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 PvuII CAG'CTG Cuts at: 2095 2652 2095 Size: 557 4970 SacI G_AGCT'C Cuts at: 2352 2352 Size: 5527 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SapI GCTCTTCn'nnn_ Cuts at: 2805 2805 Size: 5527 SbfI CC_TGCA'GG Cuts at: 2879 2879 Size: 5527 ScaI AGT'ACT Cuts at: 3775 5487 3775 Size: 1712 3815 SfcI C'TryA_G Cuts at: 826 1045 2875 4010 4691 4882 826 Size: 219 1830 1135 681 191 1471 Fragments arranged by size: 1830 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2854 1872 Size: 982 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3631 4532 4794 820 Size: 197 926 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5527 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 2153 3451 5 Size: 47 2101 1298 2081 Fragments arranged by size: 2101 2081 1298 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5527 StyI C'CwwG_G Cuts at: 513 1065 2391 2565 2966 513 Size: 552 1326 174 401 3074 Fragments arranged by size: 3074 1326 552 401 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3554 3571 3724 3909 4354 325 Size: 1255 1974 17 153 185 445 1498 Fragments arranged by size: 1974 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2646 3773 5485 Size: 274 80 33 51 1443 669 1127 1712 Cuts at: 5485 96 Size: 138 Fragments arranged by size: 1712 1443 1127 669 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 2615 2647 5487 5519 2072 Size: 32 511 32 2840 32 2080 Fragments arranged by size: 2840 2080 511 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2533 1316 Size: 1217 4310 XbaI T'CTAG_A Cuts at: 2863 2863 Size: 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5527 XmaI C'CCGG_G Cuts at: 1870 2852 1870 Size: 982 4545 XmnI GAAnn'nnTTC Cuts at: 3656 3656 Size: 5527 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2532 3335 276 Size: 53 83 186 936 998 803 2468 Fragments arranged by size: 2468 998 936 803 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI