(Circular) (Six-base) MAPSORT of: pFN21AB7707.seq Check: 4308 from: 1 to: 5962 pFN21AB7707 5962 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2387 2387 Size: 5962 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2119 4025 5409 5677 1632 Size: 487 1906 1384 268 1917 Fragments arranged by size: 1917 1906 1384 487 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 2875 4691 2875 Size: 1816 4146 AleI CACnn'nnGTG Cuts at: 1166 2895 2986 1166 Size: 1729 91 4142 Fragments arranged by size: 4142 1729 91 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2480 1184 Size: 1296 4666 AlwNI CAG_nnn'CTG Cuts at: 2713 2809 2923 3705 5277 2713 Size: 96 114 782 1572 3398 Fragments arranged by size: 3398 1572 782 114 96 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2967 3287 1870 Size: 73 1024 320 4545 Fragments arranged by size: 4545 1024 320 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2268 2301 2268 Size: 33 5929 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3283 4738 618 Size: 325 217 627 1496 1455 1842 Fragments arranged by size: 1842 1496 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3220 3281 1391 Size: 1829 61 4072 Fragments arranged by size: 4072 1829 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2531 2595 928 Size: 971 162 470 64 4295 Fragments arranged by size: 4295 971 470 162 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4153 4187 1903 Size: 34 50 34 2132 34 3678 Fragments arranged by size: 3678 2132 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2269 3854 5064 2269 Size: 1585 1210 3167 Fragments arranged by size: 3167 1585 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 2835 3379 2835 Size: 544 5418 Bme1580I G_kGCm'C Cuts at: 1790 4023 5179 1790 Size: 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 BmgBI CAC'GTC Cuts at: 1318 2646 1318 Size: 1328 4634 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2891 3162 4622 1239 Size: 1652 271 1460 2579 Fragments arranged by size: 2579 1652 1460 271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4087 4952 5250 4087 Size: 865 298 4799 Fragments arranged by size: 4799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2225 2229 2957 4625 882 Size: 617 726 4 728 1668 2219 Fragments arranged by size: 2219 1668 728 726 617 4 BsaAI yAC'GTr Cuts at: 493 1204 2584 3753 493 Size: 711 1380 1169 2702 Fragments arranged by size: 2702 1380 1169 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2789 3769 4151 4786 Size: 53 83 186 936 1256 980 382 635 Cuts at: 4786 275 Size: 1451 Fragments arranged by size: 1451 1256 980 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2021 2051 2205 2235 1178 Size: 30 165 30 618 30 154 30 4905 Fragments arranged by size: 4905 618 165 154 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5962 BseYI C'CCAG_C Cuts at: 5165 5661 5165 Size: 496 5466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2404 1163 Size: 573 668 4721 Fragments arranged by size: 4721 668 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3056 3412 4173 5201 1540 Size: 275 186 1055 356 761 1028 2301 Fragments arranged by size: 2301 1055 1028 761 356 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 4023 4108 5179 2090 Size: 1933 85 1071 2873 Fragments arranged by size: 2873 1933 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 BsmBI CGTCTCn'nnnn_ Cuts at: 2636 2636 Size: 5962 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 3220 3281 4023 4108 5179 1391 Size: 399 300 1130 61 742 85 1071 2174 Fragments arranged by size: 2174 1130 1071 742 399 300 85 61 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2387 2784 3317 844 Size: 1543 397 533 3489 Fragments arranged by size: 3489 1543 533 397 BsrBI CCG'CTC Cuts at: 1394 1823 2327 2931 3849 1394 Size: 429 504 604 918 3507 Fragments arranged by size: 3507 918 604 504 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3055 4606 4793 1700 Size: 110 1245 1551 187 2869 Fragments arranged by size: 2869 1551 1245 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 3241 4022 5034 3241 Size: 781 1012 4169 Fragments arranged by size: 4169 1012 781 BstAPI GCAn_nnn'nTGC Cuts at: 794 2671 2713 2809 794 Size: 1877 42 96 3947 Fragments arranged by size: 3947 1877 96 42 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 2107 2653 2897 1184 Size: 923 546 244 4249 Fragments arranged by size: 4249 923 546 244 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2584 2988 2047 Size: 537 404 5021 Fragments arranged by size: 5021 537 404 BstYI r'GATC_y Cuts at: 1972 3108 3292 3659 4044 4061 5513 5599 5611 Size: 1136 184 367 385 17 1452 86 12 Cuts at: 5611 5957 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1136 385 367 346 184 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3031 3192 3031 Size: 161 5801 BtgI C'CryG_G Cuts at: 513 1065 2040 2280 513 Size: 552 975 240 4195 Fragments arranged by size: 4195 975 552 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2682 3478 4272 4292 2682 Size: 796 794 20 4352 Fragments arranged by size: 4352 796 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 3053 3057 Size: 54 1425 50 275 18 498 725 4 Cuts at: 3057 3409 4298 5927 8 Size: 352 889 1629 43 Fragments arranged by size: 1629 1425 889 725 498 352 275 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3053 3409 1537 Size: 275 1241 356 4090 Fragments arranged by size: 4090 1241 356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3892 1353 Size: 2539 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2891 3162 4025 4622 5409 5677 Size: 393 487 772 271 863 597 787 268 Cuts at: 5677 1239 Size: 1524 Fragments arranged by size: 1524 863 787 772 597 487 393 271 268 EcoO109I rG'GnC_Cy Cuts at: 2119 2119 Size: 5962 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FseI GG_CCGG'CC Cuts at: 3059 3059 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 5109 1678 Size: 3431 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2483 2515 2593 Size: 32 135 32 449 32 430 32 78 Cuts at: 2593 2625 2861 2893 4603 4635 4677 4709 1373 Size: 32 236 32 1710 32 42 32 2626 Fragments arranged by size: 2626 1710 449 430 236 135 78 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 2556 748 Size: 1808 4154 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 5076 5260 1305 Size: 810 2961 184 2007 Fragments arranged by size: 2961 2007 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2462 2503 2895 2986 3920 4279 4438 Size: 648 1296 41 392 91 934 359 159 Cuts at: 4438 518 Size: 2042 Fragments arranged by size: 2042 1296 934 648 392 359 159 91 41 MspA1I CmG'CkG Cuts at: 1606 2022 2080 2282 2842 3378 4055 5203 5448 Size: 416 58 202 560 536 677 1148 245 Cuts at: 5448 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 560 536 416 245 202 58 NaeI GCC'GGC Cuts at: 3057 3057 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5410 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NgoMIV G'CCGG_C Cuts at: 3055 3055 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2467 3091 3320 4865 1132 Size: 97 1238 624 229 1545 2229 Fragments arranged by size: 2229 1545 1238 624 229 97 PasI CC'CwG_GG Cuts at: 1348 3043 1348 Size: 1695 4267 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2923 1108 Size: 1815 4147 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2119 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 2241 1613 Size: 628 5334 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 2256 2718 2725 2805 3314 830 Size: 1426 462 7 80 509 3478 Fragments arranged by size: 3478 1426 509 462 80 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2080 2842 2080 Size: 762 5200 SacII CC_GC'GG Cuts at: 2283 2283 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 2805 3314 2805 Size: 509 5453 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2252 2714 2721 2801 3310 4445 5126 Size: 219 1207 462 7 80 509 1135 681 Cuts at: 5126 5317 826 Size: 191 1471 Fragments arranged by size: 1471 1207 1135 681 509 462 219 191 80 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4066 4967 5229 820 Size: 197 926 2123 901 262 1553 Fragments arranged by size: 2123 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 3401 513 Size: 552 2336 3074 Fragments arranged by size: 3074 2336 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2349 3989 4006 4159 4344 4789 325 Size: 1255 769 1640 17 153 185 445 1498 Fragments arranged by size: 1640 1498 1255 769 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4208 5920 96 Size: 274 80 33 51 1443 2231 1712 138 Fragments arranged by size: 2231 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2478 2510 5922 5954 2478 Size: 32 3412 32 2486 Fragments arranged by size: 3412 2486 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2047 2047 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 3025 4091 3025 Size: 1066 4896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI