################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsiWI BsmI BspDI BspLU11I BsrBI BssHII BssSI BstBI BstEII BstPI CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MunI NdeI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SpeI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 1335 GACGTC GACGT^C AccI 686 GTATAC GT^[AC][GT]AC AccIII 921 TCCGGA T^CCGGA AcsI 2207 AAATTC [GA]^AATT[CT] AlwNI 2026 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 2207 AAATTC [GA]^AATT[CT] BamHI 215 GGATCC G^GATCC BbeI 8 GGCGCC GGCGC^C BbrPI 2113 CACGTG CAC^GTG BbvCI 1680 CCTCAGC CCTCAGC(-5/-2) BcgI 504 GCAGTTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1925 TGATCA T^GATCA BsaI 523 GAGACC GGTCTC(1/5) BsaWI 921 TCCGGA [AT]^CCGG[AT] BseAI 921 TCCGGA T^CCGGA BsiEI 81 CGGTCG CG[GA][CT]^CG BsmBI 52 GAGACG CGTCTC(1/5) Bsp1407I 1776 TGTACA T^GTACA BspEI 921 TCCGGA T^CCGGA BspMI 2942 ACCTGC ACCTGC(4/8) BsrDI 776 CATTGC GCAATG(2/0) BsrGI 1776 TGTACA T^GTACA Bst1107I 686 GTATAC GTA^TAC BstAPI 2025 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 656 CCACTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 686 GTATAC GTA^TAC DraI 2185 TTTAAA TTT^AAA EcoRV 607 GATATC GAT^ATC FbaI 1925 TGATCA T^GATCA KasI 8 GGCGCC G^GCGCC MroI 921 TCCGGA T^CCGGA NaeI 59 GCCGGC GCC^GGC NarI 8 GGCGCC GG^CGCC NcoI 840 CCATGG C^CATGG NgoMIV 59 GCCGGC G^CCGGC NheI 2758 GCTAGC G^CTAGC PaeR7I 2583 CTCGAG C^TCGAG PmaCI 2113 CACGTG CAC^GTG PmlI 2113 CACGTG CAC^GTG PvuII 105 CAGCTG CAG^CTG SfoI 8 GGCGCC GGC^GCC SnaBI 424 TACGTA TAC^GTA SphI 2124 GCATGC GCATG^C SspBI 1776 TGTACA T^GTACA XhoI 2583 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 31 CGCG CG^CG 56 CGCG AcyI 8 GGCGCC G[GA]^CG[CT]C 1335 GACGTC Alw44I 1158 GTGCAC G^TGCAC 2848 GTGCAC ApaLI 1158 GTGCAC G^TGCAC 2848 GTGCAC Asp700I 1089 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1797 GAAGCCTTTC BfaI 647 CTAG C^TAG 2759 CTAG BsaAI 424 TACGTA [CT]AC^GT[GA] 2113 CACGTG BsaHI 8 GGCGCC G[GA]^CG[CT]C 1335 GACGTC BseRI 1355 CTCCTC GAGGAG(10/8) 1765 GAGGAG BspHI 470 TCATGA T^CATGA 807 TCATGA BsrFI 59 GCCGGC [GA]^CCGG[CT] 338 ACCGGC BstUI 31 CGCG CG^CG 56 CGCG BstYI 215 GGATCC [GA]^GATC[CT] 377 AGATCC Cfr10I 59 GCCGGC [GA]^CCGG[CT] 338 ACCGGC DsaI 417 CCGTGG C^C[GA][CT]GG 840 CCATGG EcoNI 1369 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2279 CCTCCCGCAGG HgaI 29 GACGC GACGC(5/10) 54 GACGC Hin1I 8 GGCGCC G[GA]^CG[CT]C 1335 GACGTC HphI 2634 GGTGA GGTGA(8/7) 2973 TCACC MaeI 647 CTAG C^TAG 2759 CTAG MflI 215 GGATCC [GA]^GATC[CT] 377 AGATCC MvnI 31 CGCG CG^CG 56 CGCG PflMI 384 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1408 CCAAACATTGG PshAI 811 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2448 GACATCTGTC RcaI 470 TCATGA T^CATGA 807 TCATGA SapI 565 GAAGAGC GCTCTTC(1/4) 342 GCTCTTC SmlI 2547 CTTGAG C^T[CT][GA]AG 2583 CTCGAG StuI 1351 AGGCCT AGG^CCT 1733 AGGCCT Van91I 384 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1408 CCAAACATTGG XcmI 171 CCAATGCAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2063 CCAACAGGGGAATGG XhoII 215 GGATCC [GA]^GATC[CT] 377 AGATCC XmnI 1089 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1797 GAAGCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AfaI AluI AlwI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmaCI XmaI