(Linear) (Six-base) MAPSORT of: KIEE3770.seq Check: 3416 from: 1 to: 2982 KIEE3770.orf With 182 enzymes: SgfI * March 22, 2012 16:44 .. AatII G_ACGT'C Cuts at: 0 1339 2982 Size: 1339 1643 AccI GT'mk_AC Cuts at: 0 687 2982 Size: 687 2295 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 330 388 1829 2034 2982 Size: 330 58 1441 205 948 Fragments arranged by size: 1441 948 330 205 58 AleI CACnn'nnGTG Cuts at: 0 661 2982 Size: 661 2321 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 304 2982 Size: 304 2678 AlwNI CAG_nnn'CTG Cuts at: 0 2031 2982 Size: 2031 951 ApaI G_GGCC'C Cuts at: 0 48 848 2835 2982 Size: 48 800 1987 147 Fragments arranged by size: 1987 800 147 48 ApaLI G'TGCA_C Cuts at: 0 1158 2848 2982 Size: 1158 1690 134 Fragments arranged by size: 1690 1158 134 ApoI r'AATT_y Cuts at: 0 2207 2982 Size: 2207 775 AvaI C'yCGr_G Cuts at: 0 41 47 142 2583 2982 Size: 41 6 95 2441 399 Fragments arranged by size: 2441 399 95 41 6 BamHI G'GATC_C Cuts at: 0 215 2982 Size: 215 2767 BanI G'GyrC_C Cuts at: 0 8 71 1108 1932 2557 2982 Size: 8 63 1037 824 625 425 Fragments arranged by size: 1037 824 625 425 63 8 BanII G_rGCy'C Cuts at: 0 48 848 2835 2982 Size: 48 800 1987 147 Fragments arranged by size: 1987 800 147 48 BbeI G_GCGC'C Cuts at: 0 12 2982 Size: 12 2970 BbsI GAAGACnn'nnnn_ Cuts at: 0 950 1096 1700 2185 2982 Size: 950 146 604 485 797 Fragments arranged by size: 950 797 604 485 146 BbvCI CC'TCA_GC Cuts at: 0 1681 2982 Size: 1681 1301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 493 527 2982 Size: 493 34 2455 Fragments arranged by size: 2455 493 34 BciVI GTATCCnnnnn_n' Cuts at: 0 239 415 1511 2982 Size: 239 176 1096 1471 Fragments arranged by size: 1471 1096 239 176 BclI T'GATC_A Cuts at: 0 1925 2982 Size: 1925 1057 BfrBI ATG'CAT Cuts at: 0 439 2128 2226 2982 Size: 439 1689 98 756 Fragments arranged by size: 1689 756 439 98 Bme1580I G_kGCm'C Cuts at: 0 48 848 1162 1321 2560 2835 2852 2982 Size: 48 800 314 159 1239 275 17 130 Fragments arranged by size: 1239 800 314 275 159 130 48 17 BmtI G_CTAG'C Cuts at: 0 2762 2982 Size: 2762 220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 483 951 2128 2545 2962 2982 Size: 358 125 468 1177 417 417 20 Fragments arranged by size: 1177 468 417 417 358 125 20 Bpu10I CC'TnA_GC Cuts at: 0 211 1681 1791 1874 2402 2982 Size: 211 1470 110 83 528 580 Fragments arranged by size: 1470 580 528 211 110 83 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2568 2982 Size: 2568 414 BsaI GGTCTCn'nnnn_ Cuts at: 0 517 2982 Size: 517 2465 BsaAI yAC'GTr Cuts at: 0 426 2115 2982 Size: 426 1689 867 Fragments arranged by size: 1689 867 426 BsaHI Gr'CG_yC Cuts at: 0 9 1336 2982 Size: 9 1327 1646 Fragments arranged by size: 1646 1327 9 BsaWI w'CCGG_w Cuts at: 0 921 2982 Size: 921 2061 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1346 1780 2982 Size: 1346 434 1202 Fragments arranged by size: 1346 1202 434 BseYI C'CCAG_C Cuts at: 0 679 1360 2038 2982 Size: 679 681 678 944 Fragments arranged by size: 944 681 679 678 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 746 1059 1222 2396 2929 2982 Size: 746 313 163 1174 533 53 Fragments arranged by size: 1174 746 533 313 163 53 BsiEI CG_ry'CG Cuts at: 0 84 2982 Size: 84 2898 BsiHKAI G_wGCw'C Cuts at: 0 1162 2114 2578 2852 2982 Size: 1162 952 464 274 130 Fragments arranged by size: 1162 952 464 274 130 BsmBI CGTCTCn'nnnn_ Cuts at: 0 46 2982 Size: 46 2936 Bsp1286I G_dGCh'C Cuts at: 0 48 848 1162 1321 2114 2560 2578 2835 Size: 48 800 314 159 793 446 18 257 Cuts at: 2835 2852 2982 Size: 17 130 Fragments arranged by size: 800 793 446 314 257 159 130 48 18 17 BspEI T'CCGG_A Cuts at: 0 921 2982 Size: 921 2061 BspHI T'CATG_A Cuts at: 0 470 807 2982 Size: 470 337 2175 Fragments arranged by size: 2175 470 337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2951 2982 Size: 2951 31 BsrDI GCAATG_nn' Cuts at: 0 775 2982 Size: 775 2207 BsrFI r'CCGG_y Cuts at: 0 59 338 2982 Size: 59 279 2644 Fragments arranged by size: 2644 279 59 BsrGI T'GTAC_A Cuts at: 0 1776 2982 Size: 1776 1206 BstAPI GCAn_nnn'nTGC Cuts at: 0 2031 2982 Size: 2031 951 BstXI CCAn_nnnn'nTGG Cuts at: 0 663 2982 Size: 663 2319 BstYI r'GATC_y Cuts at: 0 215 377 2982 Size: 215 162 2605 Fragments arranged by size: 2605 215 162 BstZ17I GTA'TAC Cuts at: 0 688 2982 Size: 688 2294 Bsu36I CC'TnA_GG Cuts at: 0 1016 1195 1276 2982 Size: 1016 179 81 1706 Fragments arranged by size: 1706 1016 179 81 BtgI C'CryG_G Cuts at: 0 417 840 2982 Size: 417 423 2142 Fragments arranged by size: 2142 423 417 BtsI GCAGTG_nn' Cuts at: 0 1076 2982 Size: 1076 1906 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 224 259 2982 Size: 224 35 2723 Fragments arranged by size: 2723 224 35 DraI TTT'AAA Cuts at: 0 2187 2982 Size: 2187 795 EaeI y'GGCC_r Cuts at: 0 92 491 1295 1956 2982 Size: 92 399 804 661 1026 Fragments arranged by size: 1026 804 661 399 92 EarI CTCTTCn'nnn_ Cuts at: 0 349 560 1551 2053 2982 Size: 349 211 991 502 929 Fragments arranged by size: 991 929 502 349 211 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 41 2982 Size: 41 2941 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 330 358 388 483 951 1829 2034 2128 Size: 330 28 30 95 468 878 205 94 Cuts at: 2128 2545 2962 2982 Size: 417 417 20 Fragments arranged by size: 878 468 417 417 330 205 95 94 30 28 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 1373 2283 2982 Size: 1373 910 699 Fragments arranged by size: 1373 910 699 EcoO109I rG'GnC_Cy Cuts at: 0 299 822 845 1820 2822 2831 2982 Size: 299 523 23 975 1002 9 151 Fragments arranged by size: 1002 975 523 299 151 23 9 EcoRV GAT'ATC Cuts at: 0 609 2982 Size: 609 2373 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2199 2982 Size: 2199 783 HaeII r_GCGC'y Cuts at: 0 12 1220 2031 2982 Size: 12 1208 811 951 Fragments arranged by size: 1208 951 811 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 514 546 618 650 955 987 1191 1223 Size: 514 32 72 32 305 32 204 32 Cuts at: 1223 1664 1696 2982 Size: 441 32 1286 Fragments arranged by size: 1286 514 441 305 204 72 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 8 2982 Size: 8 2974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 256 434 776 2087 2982 Size: 256 178 342 1311 895 Fragments arranged by size: 1311 895 342 256 178 MscI TGG'CCA Cuts at: 0 493 1297 1958 2982 Size: 493 804 661 1024 Fragments arranged by size: 1024 804 661 493 MslI CAynn'nnrTG Cuts at: 0 661 959 1127 1246 2372 2982 Size: 661 298 168 119 1126 610 Fragments arranged by size: 1126 661 610 298 168 119 MspA1I CmG'CkG Cuts at: 0 80 107 1536 2982 Size: 80 27 1429 1446 Fragments arranged by size: 1446 1429 80 27 NaeI GCC'GGC Cuts at: 0 61 2982 Size: 61 2921 NarI GG'CG_CC Cuts at: 0 9 2982 Size: 9 2973 NcoI C'CATG_G Cuts at: 0 840 2982 Size: 840 2142 NgoMIV G'CCGG_C Cuts at: 0 59 2982 Size: 59 2923 NheI G'CTAG_C Cuts at: 0 2758 2982 Size: 2758 224 NsiI A_TGCA'T Cuts at: 0 441 2130 2228 2982 Size: 441 1689 98 754 Fragments arranged by size: 1689 754 441 98 NspI r_CATG'y Cuts at: 0 1085 1245 1701 1777 2128 2230 2249 2982 Size: 1085 160 456 76 351 102 19 733 Fragments arranged by size: 1085 733 456 351 160 102 76 19 PasI CC'CwG_GG Cuts at: 0 2701 2982 Size: 2701 281 PflMI CCAn_nnn'nTGG Cuts at: 0 390 1414 2982 Size: 390 1024 1568 Fragments arranged by size: 1568 1024 390 PfoI T'CCnGG_A Cuts at: 0 1609 2564 2982 Size: 1609 955 418 Fragments arranged by size: 1609 955 418 PmlI CAC'GTG Cuts at: 0 2115 2982 Size: 2115 867 PpuMI rG'GwC_Cy Cuts at: 0 299 822 2822 2982 Size: 299 523 2000 160 Fragments arranged by size: 2000 523 299 160 PshAI GACnn'nnGTC Cuts at: 0 815 2452 2982 Size: 815 1637 530 Fragments arranged by size: 1637 815 530 PspOMI G'GGCC_C Cuts at: 0 44 844 2831 2982 Size: 44 800 1987 151 Fragments arranged by size: 1987 800 151 44 PspXI vC'TCGA_Gb Cuts at: 0 2583 2982 Size: 2583 399 PstI C_TGCA'G Cuts at: 0 434 634 1741 2024 2982 Size: 434 200 1107 283 958 Fragments arranged by size: 1107 958 434 283 200 PvuII CAG'CTG Cuts at: 0 107 2982 Size: 107 2875 SanDI GG'GwC_CC Cuts at: 0 299 2982 Size: 299 2683 SapI GCTCTTCn'nnn_ Cuts at: 0 349 560 2982 Size: 349 211 2422 Fragments arranged by size: 2422 349 211 SfcI C'TryA_G Cuts at: 0 120 430 533 630 1347 1705 1737 2020 Size: 120 310 103 97 717 358 32 283 Cuts at: 2020 2982 Size: 962 Fragments arranged by size: 962 717 358 310 283 120 103 97 32 SfoI GGC'GCC Cuts at: 0 10 2982 Size: 10 2972 SmaI CCC'GGG Cuts at: 0 43 49 144 2982 Size: 43 6 95 2838 Fragments arranged by size: 2838 95 43 6 SmlI C'TyrA_G Cuts at: 0 2547 2583 2982 Size: 2547 36 399 Fragments arranged by size: 2547 399 36 SnaBI TAC'GTA Cuts at: 0 426 2982 Size: 426 2556 SphI G_CATG'C Cuts at: 0 2128 2982 Size: 2128 854 SrfI GCCC'GGGC Cuts at: 0 43 2982 Size: 43 2939 StuI AGG'CCT Cuts at: 0 1353 1735 2982 Size: 1353 382 1247 Fragments arranged by size: 1353 1247 382 StyI C'CwwG_G Cuts at: 0 389 840 1047 1936 2982 Size: 389 451 207 889 1046 Fragments arranged by size: 1046 889 451 389 207 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 177 967 2982 Size: 177 790 2015 Fragments arranged by size: 2015 790 177 TatI w'GTAC_w Cuts at: 0 109 1776 2982 Size: 109 1667 1206 Fragments arranged by size: 1667 1206 109 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 178 2070 2982 Size: 178 1892 912 Fragments arranged by size: 1892 912 178 XhoI C'TCGA_G Cuts at: 0 2583 2982 Size: 2583 399 XmaI C'CCGG_G Cuts at: 0 41 47 142 2982 Size: 41 6 95 2840 Fragments arranged by size: 2840 95 41 6 XmnI GAAnn'nnTTC Cuts at: 0 1093 1801 2982 Size: 1093 708 1181 Fragments arranged by size: 1181 1093 708 ZraI GAC'GTC Cuts at: 0 1337 2982 Size: 1337 1645 Enzymes that do cut: AatII AccI AcuI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI Bme1580I BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SanDI SapI SfcI SfoI SmaI SmlI SnaBI SphI SrfI StuI StyI TaqII TatI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BglI BglII BlpI BmgBI BmrI BplI BsaBI BsaXI BsiWI BsmI BsrBI BssHII BssSI BstBI BstEII BtgZI ClaI DraIII DrdI EagI EcoICRI EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NotI NruI PacI PciI PmeI PpiI PsiI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SpeI SspI SwaI TstI Tth111I XbaI