(Linear) (Six-base) MAPSORT of: FLJ00065.seq  Check: 3708  from: 1  to: 552



FLJ00065.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     394     552 
   Size:        124     270     158

  Fragments arranged by size: 

                 270     158     124
 
AleI CACnn'nnGTG

Cuts at:      0     164     552 
   Size:        164     388
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     524     552 
   Size:        524      28
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      25      57     552 
   Size:         25      32     495

  Fragments arranged by size: 

                 495      32      25
 
AvaI C'yCGr_G

Cuts at:      0      76     552 
   Size:         76     476
 
AvrII C'CTAG_G

Cuts at:      0     547     552 
   Size:        547       5
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     203     552 
   Size:        203     349
 
BbvCI CC'TCA_GC

Cuts at:      0     130     552 
   Size:        130     422
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     552 
   Size:         49     503
 
Bpu10I CC'TnA_GC

Cuts at:      0     130     552 
   Size:        130     422
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     481     552 
   Size:         37     444      71

  Fragments arranged by size: 

                 444      71      37
 
BseYI C'CCAG_C

Cuts at:      0     222     232     275     330     552 
   Size:        222      10      43      55     222

  Fragments arranged by size: 

                 222     222      55      43      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     484     552 
   Size:        466      18      68

  Fragments arranged by size: 

                 466      68      18
 
BsrDI GCAATG_nn'

Cuts at:      0     300     416     552 
   Size:        300     116     136

  Fragments arranged by size: 

                 300     136     116
 
BsrFI r'CCGG_y

Cuts at:      0      63     434     552 
   Size:         63     371     118

  Fragments arranged by size: 

                 371     118      63
 
BstEII G'GTnAC_C

Cuts at:      0      66     552 
   Size:         66     486
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     166     552 
   Size:        166     386
 
BstYI r'GATC_y

Cuts at:      0     485     552 
   Size:        485      67
 
BtsI GCAGTG_nn'

Cuts at:      0     159     376     552 
   Size:        159     217     176

  Fragments arranged by size: 

                 217     176     159
 
EarI CTCTTCn'nnn_

Cuts at:      0     151     499     518     552 
   Size:        151     348      19      34

  Fragments arranged by size: 

                 348     151      34      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     124     394     552 
   Size:         49      75     270     158

  Fragments arranged by size: 

                 270     158      75      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     544     552 
   Size:        544       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     128     160     552 
   Size:        128      32     392

  Fragments arranged by size: 

                 392     128      32
 
HindIII A'AGCT_T

Cuts at:      0     406     552 
   Size:        406     146
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      69     552 
   Size:         69     483
 
MslI CAynn'nnrTG

Cuts at:      0      12     164     552 
   Size:         12     152     388

  Fragments arranged by size: 

                 388     152      12
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      55     552 
   Size:         55     497
 
SapI GCTCTTCn'nnn_

Cuts at:      0     151     552 
   Size:        151     401
 
SmaI CCC'GGG

Cuts at:      0      78     552 
   Size:         78     474
 
StyI C'CwwG_G

Cuts at:      0     237     547     552 
   Size:        237     310       5

  Fragments arranged by size: 

                 310     237       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     172     552 
   Size:        172     380
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      52     552 
   Size:         52     500
 
XmaI C'CCGG_G

Cuts at:      0      76     552 
   Size:         76     476
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     AloI     AvaI    AvrII     BbsI    BbvCI
     BpmI   Bpu10I    BseRI    BseYI     BsgI    BsrDI    BsrFI   BstEII
    BstXI    BstYI     BtsI     EarI  Eco57MI EcoO109I    Hin4I  HindIII
     MmeI     MslI    PflMI     SapI     SmaI     StyI    TaqII     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     BaeI    BamHI     BanI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI