(Circular) (Six-base) MAPSORT of: pFN21AB5394.seq Check: 5134 from: 1 to: 6484 pFN21AB5394 6484 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3690 4547 5931 6199 1632 Size: 2058 857 1384 268 1917 Fragments arranged by size: 2058 1917 1384 857 268 AfeI AGC'GCT Cuts at: 3313 3313 Size: 6484 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 2385 2724 3096 5383 6360 1203 Size: 1182 339 372 2287 977 1327 Fragments arranged by size: 2287 1327 1182 977 372 339 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 2413 5213 2413 Size: 2800 3684 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2201 1184 Size: 1017 5467 AlwNI CAG_nnn'CTG Cuts at: 2101 2356 2371 3506 4227 5799 2101 Size: 255 15 1135 721 1572 2786 Fragments arranged by size: 2786 1572 1135 721 255 15 ApaLI G'TGCA_C Cuts at: 4541 5697 4541 Size: 1156 5328 ApoI r'AATT_y Cuts at: 1493 2152 3793 4019 4171 6280 1493 Size: 659 1641 226 152 2109 1697 Fragments arranged by size: 2109 1697 1641 659 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2927 2975 3145 3758 3809 1870 Size: 73 984 48 170 613 51 4545 Fragments arranged by size: 4545 984 613 170 73 51 48 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2177 2466 2559 3223 3323 Size: 325 217 627 390 289 93 664 100 Cuts at: 3323 3515 3805 5260 618 Size: 192 290 1455 1842 Fragments arranged by size: 1842 1455 664 627 390 325 290 289 217 192 100 93 BanII G_rGCy'C Cuts at: 1391 2280 2631 2894 3215 3270 3309 3427 3803 Size: 889 351 263 321 55 39 118 376 Cuts at: 3803 1391 Size: 4072 Fragments arranged by size: 4072 889 376 351 321 263 118 55 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3745 928 Size: 971 1846 3667 Fragments arranged by size: 3667 1846 971 BbvCI CC'TCA_GC Cuts at: 3080 3080 Size: 6484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3369 3403 4675 4709 1903 Size: 34 1432 34 1272 34 3678 Fragments arranged by size: 3678 1432 1272 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2284 2966 5095 Size: 107 122 71 631 605 612 682 2129 Cuts at: 5095 136 Size: 1525 Fragments arranged by size: 2129 1525 682 631 612 605 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 2180 2464 3326 4545 5701 1790 Size: 390 284 862 1219 1156 2573 Fragments arranged by size: 2573 1219 1156 862 390 284 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2548 2853 3127 Size: 416 53 83 399 24 1131 305 274 Cuts at: 3127 3776 4215 5173 442 Size: 649 439 958 1753 Fragments arranged by size: 1753 1131 958 649 439 416 399 305 274 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2768 2834 2886 3441 3448 3755 5144 Size: 839 690 66 52 555 7 307 1389 Cuts at: 5144 1239 Size: 2579 Fragments arranged by size: 2579 1389 839 690 555 307 66 52 7 Bpu10I CC'TnA_GC Cuts at: 3080 3080 Size: 6484 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2558 3611 4609 5474 5772 2558 Size: 1053 998 865 298 3270 Fragments arranged by size: 3270 1053 998 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2111 3485 5147 882 Size: 617 612 1374 1662 2219 Fragments arranged by size: 2219 1662 1374 617 612 BsaAI yAC'GTr Cuts at: 493 1204 2388 4275 493 Size: 711 1184 1887 2702 Fragments arranged by size: 2702 1887 1184 711 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3750 4291 4673 5308 Size: 53 83 186 936 2217 541 382 635 Cuts at: 5308 275 Size: 1451 Fragments arranged by size: 2217 1451 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2199 3009 4917 5315 5589 5736 6147 2199 Size: 810 1908 398 274 147 411 2536 Fragments arranged by size: 2536 1908 810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 2672 2702 1178 Size: 30 165 30 795 30 444 30 4960 Fragments arranged by size: 4960 795 444 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2636 2639 2669 2693 1186 Size: 1450 3 30 24 4977 Fragments arranged by size: 4977 1450 30 24 3 BseYI C'CCAG_C Cuts at: 2101 2853 3308 3344 3499 3585 5687 6183 2101 Size: 752 455 36 155 86 2102 496 2402 Fragments arranged by size: 2402 2102 752 496 455 155 86 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2078 1163 Size: 573 342 5569 Fragments arranged by size: 5569 573 342 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2961 3357 3934 4695 5723 1540 Size: 275 186 960 396 577 761 1028 2301 Fragments arranged by size: 2301 1028 960 761 577 396 275 186 BsiHKAI G_wGCw'C Cuts at: 2631 4545 4630 5701 2631 Size: 1914 85 1071 3414 Fragments arranged by size: 3414 1914 1071 85 BsmI GAATG_Cn' Cuts at: 3995 4088 3995 Size: 93 6391 BsmBI CGTCTCn'nnnn_ Cuts at: 3434 3434 Size: 6484 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2180 2280 2464 2631 2894 3215 3270 Size: 399 390 100 184 167 263 321 55 Cuts at: 3270 3309 3326 3427 3803 4545 4630 5701 1391 Size: 39 17 101 376 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 376 321 263 184 167 101 100 85 55 39 17 BspEI T'CCGG_A Cuts at: 2199 3009 6147 2199 Size: 810 3138 2536 Fragments arranged by size: 3138 2536 810 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2337 2610 3421 3561 3839 844 Size: 1493 273 811 140 278 3489 Fragments arranged by size: 3489 1493 811 278 273 140 BsrBI CCG'CTC Cuts at: 1394 1823 4371 1394 Size: 429 2548 3507 Fragments arranged by size: 3507 2548 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2421 4979 5153 65 Size: 1183 1173 2558 174 1396 Fragments arranged by size: 2558 1396 1183 1173 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2956 2960 3284 5128 5315 1700 Size: 110 1146 4 324 1844 187 2869 Fragments arranged by size: 2869 1844 1146 324 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 2076 1925 Size: 151 6333 BssSI C'ACGA_G Cuts at: 2231 2945 4544 5556 2231 Size: 714 1599 1012 3159 Fragments arranged by size: 3159 1599 1012 714 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 3175 3175 Size: 6484 BstYI r'GATC_y Cuts at: 1972 2194 2668 3075 3814 4181 4566 4583 6035 Size: 222 474 407 739 367 385 17 1452 Cuts at: 6035 6121 6133 6479 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 739 474 407 385 367 346 222 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2379 3251 3519 3745 513 Size: 552 939 375 872 268 226 3252 Fragments arranged by size: 3252 939 872 552 375 268 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2722 487 Size: 683 56 1496 4249 Fragments arranged by size: 4249 1496 683 56 BtsI GCAGTG_nn' Cuts at: 2147 3538 4000 4794 4814 2147 Size: 1391 462 794 20 3817 Fragments arranged by size: 3817 1391 794 462 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3101 5491 809 Size: 507 1785 2390 1802 Fragments arranged by size: 2390 1802 1785 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2958 3354 3600 Size: 54 1425 50 275 18 1128 396 246 Cuts at: 3600 3931 4820 6449 8 Size: 331 889 1629 43 Fragments arranged by size: 1629 1425 1128 889 396 331 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2958 3354 3931 1537 Size: 275 1146 396 577 4090 Fragments arranged by size: 4090 1146 577 396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3477 3666 4414 1353 Size: 2124 189 748 3423 Fragments arranged by size: 3423 2124 748 189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3021 5071 5445 5591 387 Size: 1064 138 1432 2050 374 146 1280 Fragments arranged by size: 2050 1432 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2629 2629 Size: 6484 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2768 2834 2886 3441 3448 3690 Size: 393 446 690 66 52 555 7 242 Cuts at: 3690 3755 4547 5144 5931 6199 1239 Size: 65 792 597 787 268 1524 Fragments arranged by size: 1524 792 787 690 597 555 446 393 268 242 66 65 52 7 EcoNI CCTnn'n_nnAGG Cuts at: 2877 3089 2877 Size: 212 6272 EcoO109I rG'GnC_Cy Cuts at: 3142 3149 3256 3365 3405 3778 3142 Size: 7 107 109 40 373 5848 Fragments arranged by size: 5848 373 109 107 40 7 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 2372 3315 3547 5631 1678 Size: 694 943 232 2084 2531 Fragments arranged by size: 2531 2084 943 694 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 2672 2704 5125 Size: 32 135 32 827 32 241 32 2421 Cuts at: 5125 5157 5199 5231 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2421 827 241 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 2941 3828 4074 669 Size: 988 281 784 219 887 246 3079 Fragments arranged by size: 3079 988 887 784 281 246 219 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5598 5782 1305 Size: 4293 184 2007 Fragments arranged by size: 4293 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4662 Fragments arranged by size: 4662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3698 4442 4801 4960 518 Size: 648 2532 744 359 159 2042 Fragments arranged by size: 2532 2042 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2167 3900 4577 5725 5970 1606 Size: 561 1733 677 1148 245 2120 Fragments arranged by size: 2120 1733 1148 677 561 245 NaeI GCC'GGC Cuts at: 2958 2962 3286 2958 Size: 4 324 6156 Fragments arranged by size: 6156 324 4 NcoI C'CATG_G Cuts at: 513 1065 2004 2379 3251 513 Size: 552 939 375 872 3746 Fragments arranged by size: 3746 939 872 552 375 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NgoMIV G'CCGG_C Cuts at: 2956 2960 3284 2956 Size: 4 324 6156 Fragments arranged by size: 6156 324 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 3842 5387 1132 Size: 97 2613 1545 2229 Fragments arranged by size: 2613 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2101 2356 1108 Size: 993 255 5236 Fragments arranged by size: 5236 993 255 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3256 3405 3778 3256 Size: 149 373 5962 Fragments arranged by size: 5962 373 149 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PstI C_TGCA'G Cuts at: 830 2190 2265 2577 2676 3624 3836 830 Size: 1360 75 312 99 948 212 3478 Fragments arranged by size: 3478 1360 948 312 212 99 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2167 2167 Size: 6484 SacI G_AGCT'C Cuts at: 2631 2631 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SanDI GG'GwC_CC Cuts at: 3405 3778 3405 Size: 373 6111 SapI GCTCTTCn'nnn_ Cuts at: 3666 3666 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 2186 2261 2573 2672 3620 3832 4967 Size: 219 1141 75 312 99 948 212 1135 Cuts at: 4967 5648 5839 826 Size: 681 191 1471 Fragments arranged by size: 1471 1141 1135 948 681 312 219 212 191 99 75 SfiI GGCCn_nnn'nGGCC Cuts at: 2966 2966 Size: 6484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 2977 3147 3811 1872 Size: 1105 170 664 4545 Fragments arranged by size: 4545 1105 664 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2537 3626 4588 5489 5751 820 Size: 197 926 594 1089 962 901 262 1553 Fragments arranged by size: 1553 1089 962 926 901 594 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 3386 1517 Size: 1869 4615 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2379 2563 3054 3251 3923 Size: 552 939 117 258 184 491 197 672 Cuts at: 3923 513 Size: 3074 Fragments arranged by size: 3074 939 672 552 491 258 197 184 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3241 4511 4528 4681 4866 5311 325 Size: 1255 1661 1270 17 153 185 445 1498 Fragments arranged by size: 1661 1498 1270 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4730 6442 96 Size: 274 80 33 51 1443 2753 1712 138 Fragments arranged by size: 2753 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2222 2254 2462 2494 3035 3067 3262 3294 6444 Size: 32 208 32 541 32 195 32 3150 Cuts at: 6444 6476 2222 Size: 32 2230 Fragments arranged by size: 3150 2230 541 208 195 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3258 3416 2011 Size: 1247 158 5079 Fragments arranged by size: 5079 1247 158 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 2975 3145 3809 1870 Size: 1105 170 664 4545 Fragments arranged by size: 4545 1105 664 170 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI