##################################
# NO CUT ENZYMES (  215 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MaeIII      MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tru9I       TseI        Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AspHI         214  GAGCAC               G[AT]GC[AT]^C
BmyI          214  GAGCAC               G[AGT]GC[ACT]^C
BpmI          137  CTGGAG               CTGGAG(16/14)
BsaBI         261  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI       214  GAGCAC               G[AT]GC[AT]^C
BsiYI         105  CCGCCATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          105  CCGCCATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         175  GTCCC                GGGAC(10/14)
Bsp1286I      214  GAGCAC               G[AGT]GC[ACT]^C
BssKI         136  CCTGG                ^CC[ACGT]GG
BstAPI        300  GCACTATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         136  CCTGG                CC^[AT]GG
BstXI         129  CCAGAAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          75  GCAGGC               GC[ACGT]^[ACGT]GC
CfoI          309  GCGC                 GCG^C
DraII          77  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I       77  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        136  CCTGG                ^CC[AT]GG
Fnu4HI         22  GCGGC                GC^[ACGT]GC
HhaI          309  GCGC                 GCG^C
HinP1I        309  GCGC                 G^CGC
HphI          121  GGTGA                GGTGA(8/7)
ItaI           22  GCGGC                GC^[ACGT]GC
MamI          261  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI          136  CCTGG                CC^[AT]GG
NlaIV          78  GGCCCC               GG[ACGT]^[ACGT]CC
PleI            4  GAGTC                GAGTC(4/5)
ScrFI         136  CCTGG                CC^[ACGT]GG
TaqI          186  TCGA                 T^CGA
TfiI          100  GAATC                G^A[AT]TC
Tsp509I       181  AATT                 ^AATT
TthHB8I       186  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BfaI           19  CTAG                 C^TAG
              281  CTAG                
Cfr13I         78  GGCCC                G^G[ACGT]CC
              191  GGGCC               
FauI          104  CCCGC                CCCGC(4/6)
              153  GCGGG               
HinfI           4  GAGTC                G^A[ACGT]TC
              100  GAATC               
MaeI           19  CTAG                 C^TAG
              281  CTAG                
MboII          94  GAAGA                GAAGA(8/7)
               97  GAAGA               
Sau96I         78  GGCCC                G^G[ACGT]CC
              191  GGGCC               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AciI        HaeIII      MnlI        MwoI