################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AviII BamHI BbrPI BcgI BclI BfrI BglI BsaBI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BstAPI BstBI BstXI ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI FspI HindIII KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1432 GACGTC GACGT^C Acc65I 1508 GGTACC G^GTACC AccI 523 GTATAC GT^[AC][GT]AC AcsI 1384 GAATTC [GA]^AATT[CT] AocI 468 CCTCAGG CC^T[ACGT]AGG Aor51HI 826 AGCGCT AGC^GCT ApaI 17 GGGCCC GGGCC^C ApoI 1384 GAATTC [GA]^AATT[CT] Asp718I 1508 GGTACC G^GTACC AvrII 549 CCTAGG C^CTAGG BalI 1544 TGGCCA TGG^CCA BbsI 1078 GTCTTC GAAGAC(2/6) BciVI 893 GTATCC GTATCC(6/5) BlnI 549 CCTAGG C^CTAGG BpuAI 1078 GTCTTC GAAGAC(2/6) BsaI 794 GGTCTC GGTCTC(1/5) BsaWI 713 TCCGGT [AT]^CCGG[AT] BseRI 381 GAGGAG GAGGAG(10/8) BssSI 1261 CACGAG CACGAG(-5/-1) Bst1107I 523 GTATAC GTA^TAC BstZ17I 523 GTATAC GTA^TAC Bsu36I 468 CCTCAGG CC^T[ACGT]AGG DrdI 1069 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1561 CTCTTC CTCTTC(1/4) Eco47III 826 AGCGCT AGC^GCT Eco81I 468 CCTCAGG CC^T[ACGT]AGG EcoNI 303 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1384 GAATTC G^AATTC EcoT22I 1448 ATGCAT ATGCA^T HincII 1306 GTTAAC GT[CT]^[GA]AC HindII 1306 GTTAAC GT[CT]^[GA]AC HinfI 247 GAGTC G^A[ACGT]TC HpaI 1306 GTTAAC GTT^AAC KpnI 1508 GGTACC GGTAC^C Ksp632I 1561 CTCTTC CTCTTC(1/4) MluNI 1544 TGGCCA TGG^CCA MscI 1544 TGGCCA TGG^CCA MseI 1307 TTAA T^TAA MslI 477 CATCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 1448 ATGCAT ATGCA^T PleI 247 GAGTC GAGTC(4/5) SacI 1264 GAGCTC GAGCT^C SapI 1560 GCTCTTC GCTCTTC(1/4) SmaI 80 CCCGGG CCC^GGG SmlI 1034 CTCAAG C^T[CT][GA]AG Tru9I 1307 TTAA T^TAA Tsp509I 1385 AATT ^AATT XmaCI 80 CCCGGG C^CCGGG XmaI 80 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwNI 506 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1192 CAGCTGCTG AspHI 75 GAGCAC G[AT]GC[AT]^C 1264 GAGCTC AspI 706 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1072 GACTTCGTC BbeI 120 GGCGCC GGCGC^C 1213 GGCGCC BbvCI 1362 CCTCAGC CCTCAGC(-5/-2) 330 GCTGAGG BfaI 550 CTAG C^TAG 832 CTAG BlpI 492 GCTGAGC GC^T[ACGT]AGC 1197 GCTGAGC Bpu1102I 492 GCTGAGC GC^T[ACGT]AGC 1197 GCTGAGC BsaAI 970 TACGTG [CT]AC^GT[GA] 1051 TACGTG BsiHKAI 75 GAGCAC G[AT]GC[AT]^C 1264 GAGCTC BsrBI 430 CCGCTC CCGCTC(-3/-3) 1123 GAGCGG BsrFI 1553 ACCGGC [GA]^CCGG[CT] 1574 ACCGGC BstEII 915 GGTTACC G^GT[ACGT]ACC 1290 GGTCACC BstPI 915 GGTTACC G^GT[ACGT]ACC 1290 GGTCACC CelII 492 GCTGAGC GC^T[ACGT]AGC 1197 GCTGAGC Cfr10I 1553 ACCGGC [GA]^CCGG[CT] 1574 ACCGGC DraII 17 GGGCCCT [GA]G^G[ACGT]CC[CT] 260 GGGGCCT DsaI 115 CCGTGG C^C[GA][CT]GG 920 CCGTGG EaeI 1025 TGGCCG [CT]^GGCC[GA] 1544 TGGCCA EcoO109I 17 GGGCCCT [GA]G^G[ACGT]CC[CT] 260 GGGGCCT EcoO65I 915 GGTTACC G^GT[ACGT]ACC 1290 GGTCACC KasI 120 GGCGCC G^GCGCC 1213 GGCGCC MaeI 550 CTAG C^TAG 832 CTAG NarI 120 GGCGCC GG^CGCC 1213 GGCGCC PflFI 706 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1072 GACTTCGTC SexAI 527 ACCAGGT A^CC[AT]GGT 1139 ACCTGGT SfoI 120 GGCGCC GGC^GCC 1213 GGCGCC TaqI 379 TCGA T^CGA 1352 TCGA Tth111I 706 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1072 GACTTCGTC TthHB8I 379 TCGA T^CGA 1352 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI AvaII BanI BanII BbvI BcnI BglII BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TseI Tsp45I TspRI XhoII