(Circular) (Six-base) MAPSORT of: pFN21AB4483.seq Check: 1474 from: 1 to: 6304 pFN21AB4483 6304 bp With 182 enzymes: SgfI * June 25, 2013 12:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3441 4114 278 Size: 53 83 186 936 1905 673 2468 Fragments arranged by size: 2468 1905 936 673 186 83 53 AccI GT'mk_AC Cuts at: 1937 2529 3647 1937 Size: 592 1118 4594 Fragments arranged by size: 4594 1118 592 Acc65I G'GTAC_C Cuts at: 3513 3625 3513 Size: 112 6192 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2489 3485 4367 5751 6019 1632 Size: 857 996 882 1384 268 1917 Fragments arranged by size: 1917 1384 996 882 857 268 AfeI AGC'GCT Cuts at: 2833 2833 Size: 6304 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 5203 6180 1203 Size: 4000 977 1327 Fragments arranged by size: 4000 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6304 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2504 1184 Size: 1320 4984 AlwNI CAG_nnn'CTG Cuts at: 2516 3202 4047 5619 2516 Size: 686 845 1572 3201 Fragments arranged by size: 3201 1572 845 686 ApaI G_GGCC'C Cuts at: 2026 2026 Size: 6304 ApaLI G'TGCA_C Cuts at: 4361 5517 4361 Size: 1156 5148 ApoI r'AATT_y Cuts at: 1493 3389 3613 3839 3991 6100 1493 Size: 1896 224 226 152 2109 1697 Fragments arranged by size: 2109 1896 1697 226 224 152 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 2085 2150 3230 3629 1870 Size: 73 142 65 1080 399 4545 Fragments arranged by size: 4545 1080 399 142 73 65 AvrII C'CTAG_G Cuts at: 2554 2554 Size: 6304 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2125 2488 2558 3218 3513 Size: 325 217 627 338 363 70 660 295 Cuts at: 3513 3625 5080 618 Size: 112 1455 1842 Fragments arranged by size: 1842 1455 660 627 363 338 325 295 217 112 70 BanII G_rGCy'C Cuts at: 1391 2026 2070 2178 2818 2887 3216 3273 3623 Size: 635 44 108 640 69 329 57 350 Cuts at: 3623 1391 Size: 4072 Fragments arranged by size: 4072 640 635 350 329 108 69 57 44 BbeI G_GCGC'C Cuts at: 2129 3222 2129 Size: 1093 5211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3076 928 Size: 971 1177 4156 Fragments arranged by size: 4156 1177 971 BbvCI CC'TCA_GC Cuts at: 2336 3368 2336 Size: 1032 5272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4495 4529 1903 Size: 34 2558 34 3678 Fragments arranged by size: 3678 2558 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2909 4196 5406 2909 Size: 1287 1210 3807 Fragments arranged by size: 3807 1287 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6304 BfrBI ATG'CAT Cuts at: 3455 3455 Size: 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4915 136 Size: 107 122 71 631 605 3243 1525 Fragments arranged by size: 3243 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2161 2617 3168 6299 2161 Size: 456 551 3131 2166 Fragments arranged by size: 3131 2166 551 456 BlpI GC'TnA_GC Cuts at: 2498 3203 3721 2498 Size: 705 518 5081 Fragments arranged by size: 5081 705 518 Bme1580I G_kGCm'C Cuts at: 1790 2026 4365 5521 1790 Size: 236 2339 1156 2573 Fragments arranged by size: 2573 2339 1156 236 BmgBI CAC'GTC Cuts at: 1318 2569 3121 1318 Size: 1251 552 4501 Fragments arranged by size: 4501 1251 552 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2812 4035 4993 Size: 416 53 83 399 24 1395 1223 958 Cuts at: 4993 442 Size: 1753 Fragments arranged by size: 1753 1395 1223 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2612 2612 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2607 2622 3125 3146 4964 1239 Size: 1184 184 15 503 21 1818 2579 Fragments arranged by size: 2579 1818 1184 503 184 21 15 Bpu10I CC'TnA_GC Cuts at: 2172 2336 3368 2172 Size: 164 1032 5108 Fragments arranged by size: 5108 1032 164 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3024 4429 5294 5592 3024 Size: 1405 865 298 3736 Fragments arranged by size: 3736 1405 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2805 4967 882 Size: 617 1306 2162 2219 Fragments arranged by size: 2219 2162 1306 617 BsaAI yAC'GTr Cuts at: 493 1204 2977 3058 4095 493 Size: 711 1773 81 1037 2702 Fragments arranged by size: 2702 1773 1037 711 81 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2126 3134 3219 3438 Size: 53 83 186 936 593 1008 85 219 Cuts at: 3438 4111 4493 5128 275 Size: 673 382 635 1451 Fragments arranged by size: 1451 1008 936 673 635 593 382 219 186 85 83 53 BsaWI w'CCGG_w Cuts at: 2718 4737 5135 5409 5556 5967 2718 Size: 2019 398 274 147 411 3055 Fragments arranged by size: 3055 2019 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6079 Fragments arranged by size: 6079 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2401 1186 Size: 1215 5089 BseYI C'CCAG_C Cuts at: 2011 2062 2398 5507 6003 2011 Size: 51 336 3109 496 2312 Fragments arranged by size: 3109 2312 496 336 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5731 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3754 4515 5543 1540 Size: 275 186 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 3273 4365 4450 5521 2084 Size: 1189 1092 85 1071 2867 Fragments arranged by size: 2867 1189 1092 1071 85 BsmI GAATG_Cn' Cuts at: 3815 3908 3815 Size: 93 6211 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2070 2084 2178 2818 2887 3216 Size: 399 236 44 14 94 640 69 329 Cuts at: 3216 3273 3623 4365 4450 5521 1391 Size: 57 350 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 399 350 329 236 94 85 69 57 44 14 BspEI T'CCGG_A Cuts at: 5967 5967 Size: 6304 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3659 844 Size: 2815 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2437 3130 4191 1394 Size: 429 614 693 1061 3507 Fragments arranged by size: 3507 1061 693 614 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4799 4973 65 Size: 1183 3551 174 1396 Fragments arranged by size: 3551 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3558 3579 4948 5135 1700 Size: 110 1748 21 1369 187 2869 Fragments arranged by size: 2869 1748 1369 187 110 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 3266 4364 5376 3266 Size: 1098 1012 4194 Fragments arranged by size: 4194 1098 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6304 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 2920 3295 1184 Size: 1736 375 4193 Fragments arranged by size: 4193 1736 375 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6304 BstYI r'GATC_y Cuts at: 1972 2161 2617 3168 3634 4001 4386 4403 5855 Size: 189 456 551 466 367 385 17 1452 Cuts at: 5855 5941 5953 6299 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 551 466 456 385 367 346 189 86 17 12 BstZ17I GTA'TAC Cuts at: 2530 2530 Size: 6304 Bsu36I CC'TnA_GG Cuts at: 2474 2474 Size: 6304 BtgI C'CryG_G Cuts at: 513 1065 2120 2925 513 Size: 552 1055 805 3892 Fragments arranged by size: 3892 1055 805 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2917 487 Size: 683 56 1691 3874 Fragments arranged by size: 3874 1691 683 56 BtsI GCAGTG_nn' Cuts at: 3593 3820 4614 4634 3593 Size: 227 794 20 5263 Fragments arranged by size: 5263 794 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3080 5311 809 Size: 507 1764 2231 1802 Fragments arranged by size: 2231 1802 1764 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3030 3549 3751 Size: 54 1425 50 275 18 1200 519 202 Cuts at: 3751 4640 6269 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1200 889 519 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3572 4234 1353 Size: 2219 662 3423 Fragments arranged by size: 3423 2219 662 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2891 4891 5265 5411 387 Size: 1064 138 1302 2000 374 146 1280 Fragments arranged by size: 2000 1302 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3271 3271 Size: 6304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2489 2607 2622 3125 3146 3485 Size: 393 791 66 118 15 503 21 339 Cuts at: 3485 4367 4964 5751 6019 1239 Size: 882 597 787 268 1524 Fragments arranged by size: 1524 882 791 787 597 503 393 339 268 118 66 21 15 EcoNI CCTnn'n_nnAGG Cuts at: 2312 2312 Size: 6304 EcoO109I rG'GnC_Cy Cuts at: 2023 2266 2023 Size: 243 6061 EcoRI G'AATT_C Cuts at: 3389 3613 3991 3389 Size: 224 378 5702 Fragments arranged by size: 5702 378 224 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 2129 2835 3222 5451 1678 Size: 451 706 387 2229 2531 Fragments arranged by size: 2531 2229 706 451 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3440 3472 4945 4977 5019 Size: 32 135 32 1868 32 1473 32 42 Cuts at: 5019 5051 1373 Size: 32 2626 Fragments arranged by size: 2626 1868 1473 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3313 3648 3894 669 Size: 988 281 1375 335 246 3079 Fragments arranged by size: 3079 1375 988 335 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3313 3894 3313 Size: 581 5723 KasI G'GCGC_C Cuts at: 2125 3218 2125 Size: 1093 5211 KpnI G_GTAC'C Cuts at: 3517 3629 3517 Size: 112 6192 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3459 5418 5602 1305 Size: 2154 1959 184 2007 Fragments arranged by size: 2154 2007 1959 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3551 10 Size: 54 1425 343 1719 2763 Fragments arranged by size: 2763 1719 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 4262 4621 4780 518 Size: 648 1320 1776 359 159 2042 Fragments arranged by size: 2042 1776 1320 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2402 2410 3139 3199 3720 4397 5545 5790 Size: 796 8 729 60 521 677 1148 245 Cuts at: 5790 1606 Size: 2120 Fragments arranged by size: 2120 1148 796 729 677 521 245 60 8 NarI GG'CG_CC Cuts at: 2126 3219 2126 Size: 1093 5211 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5752 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NsiI A_TGCA'T Cuts at: 3457 3457 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 3662 5207 1132 Size: 97 2433 1545 2229 Fragments arranged by size: 2433 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2636 2809 1348 Size: 1288 173 4843 Fragments arranged by size: 4843 1288 173 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6304 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2351 2383 2973 3005 4373 4405 5886 5918 2351 Size: 32 590 32 1368 32 1481 32 2737 Fragments arranged by size: 2737 1481 1368 590 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PspOMI G'GGCC_C Cuts at: 2022 2022 Size: 6304 PstI C_TGCA'G Cuts at: 830 2246 2332 3588 3656 830 Size: 1416 86 1256 68 3478 Fragments arranged by size: 3478 1416 1256 86 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2402 2410 3199 2402 Size: 8 789 5507 Fragments arranged by size: 5507 789 8 SacI G_AGCT'C Cuts at: 3273 3273 Size: 6304 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SapI GCTCTTCn'nnn_ Cuts at: 3572 3572 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SexAI A'CCwGG_T Cuts at: 2532 3144 2532 Size: 612 5692 SfcI C'TryA_G Cuts at: 826 1045 2242 2328 2450 3584 3652 4787 5468 Size: 219 1197 86 122 1134 68 1135 681 Cuts at: 5468 5659 826 Size: 191 1471 Fragments arranged by size: 1471 1197 1135 1134 681 219 191 122 86 68 SfoI GGC'GCC Cuts at: 2127 3220 2127 Size: 1093 5211 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 2087 3631 1872 Size: 215 1544 4545 Fragments arranged by size: 4545 1544 215 SmlI C'TyrA_G Cuts at: 820 1017 1943 3039 4408 5309 5571 820 Size: 197 926 1096 1369 901 262 1553 Fragments arranged by size: 1553 1369 1096 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 2318 2375 3228 1517 Size: 801 57 853 4593 Fragments arranged by size: 4593 853 801 57 StyI C'CwwG_G Cuts at: 513 1065 2313 2554 3473 3743 513 Size: 552 1248 241 919 270 3074 Fragments arranged by size: 3074 1248 919 552 270 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3055 4331 4348 4501 4686 5131 325 Size: 1255 1475 1276 17 153 185 445 1498 Fragments arranged by size: 1498 1475 1276 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2820 4550 6262 Size: 274 80 33 51 1443 843 1730 1712 Cuts at: 6262 96 Size: 138 Fragments arranged by size: 1730 1712 1443 843 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6264 6296 6264 Size: 32 6272 Tth111I GACn'n_nGTC Cuts at: 1316 2714 3080 1316 Size: 1398 366 4540 Fragments arranged by size: 4540 1398 366 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 2085 3629 1870 Size: 215 1544 4545 Fragments arranged by size: 4545 1544 215 XmnI GAAnn'nnTTC Cuts at: 4433 4433 Size: 6304 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3439 4112 276 Size: 53 83 186 936 1905 673 2468 Fragments arranged by size: 2468 1905 936 673 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BsiWI BsmBI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI