(Linear) (Six-base) MAPSORT of: KIBB4483.seq Check: 557 from: 1 to: 1599 KIBB4483.orf With 182 enzymes: SgfI * June 25, 2013 10:30 .. AatII G_ACGT'C Cuts at: 0 1436 1599 Size: 1436 163 AccI GT'mk_AC Cuts at: 0 524 1599 Size: 524 1075 Acc65I G'GTAC_C Cuts at: 0 1508 1599 Size: 1508 91 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1480 1599 Size: 484 996 119 Fragments arranged by size: 996 484 119 AfeI AGC'GCT Cuts at: 0 828 1599 Size: 828 771 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 499 1599 Size: 499 1100 AlwNI CAG_nnn'CTG Cuts at: 0 511 1197 1599 Size: 511 686 402 Fragments arranged by size: 686 511 402 ApaI G_GGCC'C Cuts at: 0 21 1599 Size: 21 1578 ApoI r'AATT_y Cuts at: 0 1384 1599 Size: 1384 215 AvaI C'yCGr_G Cuts at: 0 80 145 1225 1599 Size: 80 65 1080 374 Fragments arranged by size: 1080 374 80 65 AvrII C'CTAG_G Cuts at: 0 549 1599 Size: 549 1050 BanI G'GyrC_C Cuts at: 0 120 483 553 1213 1508 1599 Size: 120 363 70 660 295 91 Fragments arranged by size: 660 363 295 120 91 70 BanII G_rGCy'C Cuts at: 0 21 65 173 813 882 1211 1268 1599 Size: 21 44 108 640 69 329 57 331 Fragments arranged by size: 640 331 329 108 69 57 44 21 BbeI G_GCGC'C Cuts at: 0 124 1217 1599 Size: 124 1093 382 Fragments arranged by size: 1093 382 124 BbsI GAAGACnn'nnnn_ Cuts at: 0 1071 1599 Size: 1071 528 BbvCI CC'TCA_GC Cuts at: 0 331 1363 1599 Size: 331 1032 236 Fragments arranged by size: 1032 331 236 BciVI GTATCCnnnnn_n' Cuts at: 0 904 1599 Size: 904 695 BfrBI ATG'CAT Cuts at: 0 1450 1599 Size: 1450 149 BglII A'GATC_T Cuts at: 0 156 612 1163 1599 Size: 156 456 551 436 Fragments arranged by size: 551 456 436 156 BlpI GC'TnA_GC Cuts at: 0 493 1198 1599 Size: 493 705 401 Fragments arranged by size: 705 493 401 Bme1580I G_kGCm'C Cuts at: 0 21 1599 Size: 21 1578 BmgBI CAC'GTC Cuts at: 0 564 1116 1599 Size: 564 552 483 Fragments arranged by size: 564 552 483 BmrI ACTGGGnnnn_n' Cuts at: 0 807 1599 Size: 807 792 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 607 1599 Size: 607 992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 602 617 1120 1141 1599 Size: 418 184 15 503 21 458 Fragments arranged by size: 503 458 418 184 21 15 Bpu10I CC'TnA_GC Cuts at: 0 167 331 1363 1599 Size: 167 164 1032 236 Fragments arranged by size: 1032 236 167 164 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1019 1599 Size: 1019 580 BsaI GGTCTCn'nnnn_ Cuts at: 0 800 1599 Size: 800 799 BsaAI yAC'GTr Cuts at: 0 972 1053 1599 Size: 972 81 546 Fragments arranged by size: 972 546 81 BsaHI Gr'CG_yC Cuts at: 0 121 1129 1214 1433 1599 Size: 121 1008 85 219 166 Fragments arranged by size: 1008 219 166 121 85 BsaWI w'CCGG_w Cuts at: 0 713 1599 Size: 713 886 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 396 1599 Size: 396 1203 BseYI C'CCAG_C Cuts at: 0 6 57 393 1599 Size: 6 51 336 1206 Fragments arranged by size: 1206 336 51 6 BsiHKAI G_wGCw'C Cuts at: 0 79 1268 1599 Size: 79 1189 331 Fragments arranged by size: 1189 331 79 Bsp1286I G_dGCh'C Cuts at: 0 21 65 79 173 813 882 1211 1268 Size: 21 44 14 94 640 69 329 57 Cuts at: 1268 1599 Size: 331 Fragments arranged by size: 640 331 329 94 69 57 44 21 14 BsrBI CCG'CTC Cuts at: 0 432 1125 1599 Size: 432 693 474 Fragments arranged by size: 693 474 432 BsrFI r'CCGG_y Cuts at: 0 1553 1574 1599 Size: 1553 21 25 Fragments arranged by size: 1553 25 21 BssSI C'ACGA_G Cuts at: 0 1261 1599 Size: 1261 338 BstEII G'GTnAC_C Cuts at: 0 915 1290 1599 Size: 915 375 309 Fragments arranged by size: 915 375 309 BstYI r'GATC_y Cuts at: 0 156 612 1163 1599 Size: 156 456 551 436 Fragments arranged by size: 551 456 436 156 BstZ17I GTA'TAC Cuts at: 0 525 1599 Size: 525 1074 Bsu36I CC'TnA_GG Cuts at: 0 469 1599 Size: 469 1130 BtgI C'CryG_G Cuts at: 0 115 920 1599 Size: 115 805 679 Fragments arranged by size: 805 679 115 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 912 1599 Size: 912 687 BtsI GCAGTG_nn' Cuts at: 0 1588 1599 Size: 1588 11 DrdI GACnn_nn'nnGTC Cuts at: 0 1075 1599 Size: 1075 524 EaeI y'GGCC_r Cuts at: 0 1025 1544 1599 Size: 1025 519 55 Fragments arranged by size: 1025 519 55 EarI CTCTTCn'nnn_ Cuts at: 0 1567 1599 Size: 1567 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 886 1599 Size: 886 713 EcoICRI GAG'CTC Cuts at: 0 1266 1599 Size: 1266 333 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 484 602 617 1120 1141 1480 1599 Size: 418 66 118 15 503 21 339 119 Fragments arranged by size: 503 418 339 119 118 66 21 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 307 1599 Size: 307 1292 EcoO109I rG'GnC_Cy Cuts at: 0 18 261 1599 Size: 18 243 1338 Fragments arranged by size: 1338 243 18 EcoRI G'AATT_C Cuts at: 0 1384 1599 Size: 1384 215 HaeII r_GCGC'y Cuts at: 0 124 830 1217 1599 Size: 124 706 387 382 Fragments arranged by size: 706 387 382 124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1435 1467 1599 Size: 1435 32 132 Fragments arranged by size: 1435 132 32 HincII GTy'rAC Cuts at: 0 1308 1599 Size: 1308 291 HpaI GTT'AAC Cuts at: 0 1308 1599 Size: 1308 291 KasI G'GCGC_C Cuts at: 0 120 1213 1599 Size: 120 1093 386 Fragments arranged by size: 1093 386 120 KpnI G_GTAC'C Cuts at: 0 1512 1599 Size: 1512 87 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1454 1599 Size: 1454 145 MscI TGG'CCA Cuts at: 0 1546 1599 Size: 1546 53 MslI CAynn'nnrTG Cuts at: 0 481 1599 Size: 481 1118 MspA1I CmG'CkG Cuts at: 0 397 405 1134 1194 1599 Size: 397 8 729 60 405 Fragments arranged by size: 729 405 397 60 8 NarI GG'CG_CC Cuts at: 0 121 1214 1599 Size: 121 1093 385 Fragments arranged by size: 1093 385 121 NsiI A_TGCA'T Cuts at: 0 1452 1599 Size: 1452 147 PasI CC'CwG_GG Cuts at: 0 631 804 1599 Size: 631 173 795 Fragments arranged by size: 795 631 173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 346 378 968 1000 1599 Size: 346 32 590 32 599 Fragments arranged by size: 599 590 346 32 32 PspOMI G'GGCC_C Cuts at: 0 17 1599 Size: 17 1582 PstI C_TGCA'G Cuts at: 0 241 327 1583 1599 Size: 241 86 1256 16 Fragments arranged by size: 1256 241 86 16 PvuII CAG'CTG Cuts at: 0 397 405 1194 1599 Size: 397 8 789 405 Fragments arranged by size: 789 405 397 8 SacI G_AGCT'C Cuts at: 0 1268 1599 Size: 1268 331 SapI GCTCTTCn'nnn_ Cuts at: 0 1567 1599 Size: 1567 32 SexAI A'CCwGG_T Cuts at: 0 527 1139 1599 Size: 527 612 460 Fragments arranged by size: 612 527 460 SfcI C'TryA_G Cuts at: 0 237 323 445 1579 1599 Size: 237 86 122 1134 20 Fragments arranged by size: 1134 237 122 86 20 SfoI GGC'GCC Cuts at: 0 122 1215 1599 Size: 122 1093 384 Fragments arranged by size: 1093 384 122 SmaI CCC'GGG Cuts at: 0 82 1599 Size: 82 1517 SmlI C'TyrA_G Cuts at: 0 1034 1599 Size: 1034 565 StuI AGG'CCT Cuts at: 0 313 370 1223 1599 Size: 313 57 853 376 Fragments arranged by size: 853 376 313 57 StyI C'CwwG_G Cuts at: 0 308 549 1468 1599 Size: 308 241 919 131 Fragments arranged by size: 919 308 241 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1050 1599 Size: 1050 549 TatI w'GTAC_w Cuts at: 0 815 1599 Size: 815 784 Tth111I GACn'n_nGTC Cuts at: 0 709 1075 1599 Size: 709 366 524 Fragments arranged by size: 709 524 366 XmaI C'CCGG_G Cuts at: 0 80 1599 Size: 80 1519 ZraI GAC'GTC Cuts at: 0 1434 1599 Size: 1434 165 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AlfI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI KasI KpnI MmeI MscI MslI MspA1I NarI NsiI PasI PpiI PspOMI PstI PvuII SacI SapI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I XmaI ZraI Enzymes that do not cut: AarI AclI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI BaeI BamHI BcgI BclI BglI BmtI BsaBI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstXI ClaI CspCI DraI DraIII EagI EcoRV FalI FseI FspI FspAI HindIII MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XcmI XhoI XmnI