Restriction map for
pFN21AE0558
(Circular) (Six-base) MAPSORT of: pFN21AE0558.seq Check: 933 from: 1 to: 5908
pFN21AE0558 5908 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3191 3191
Size: 5908
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2733 2988 3718 278
Size: 53 83 186 936 1197 255 730 2468
Fragments arranged by size:
2468 1197 936 730 255 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2028 2406 3251 1937
Size: 91 378 845 4594
Fragments arranged by size:
4594 845 378 91
Acc65I G'GTAC_C
Cuts at: 3229 3229
Size: 5908
AclI AA'CG_TT
Cuts at: 1563 4035 4408 1563
Size: 2472 373 3063
Fragments arranged by size:
3063 2472 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3971 5355 5623 1632
Size: 2339 1384 268 1917
Fragments arranged by size:
2339 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5711
AflIII A'CryG_T
Cuts at: 1203 4807 5784 1203
Size: 3604 977 1327
Fragments arranged by size:
3604 1327 977
AgeI A'CCGG_T
Cuts at: 4739 4739
Size: 5908
AhdI GACnn_n'nnGTC
Cuts at: 4637 4637
Size: 5908
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5908
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5908
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 2777 2809 2309
Size: 32 436 32 5408
Fragments arranged by size:
5408 436 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2107 2314 2578 2581 3651 5223 2107
Size: 207 264 3 1070 1572 2792
Fragments arranged by size:
2792 1572 1070 264 207 3
ApaI G_GGCC'C
Cuts at: 2241 2866 2241
Size: 625 5283
ApaLI G'TGCA_C
Cuts at: 3122 3167 3965 5121 3122
Size: 45 798 1156 3909
Fragments arranged by size:
3909 1156 798 45
ApoI r'AATT_y
Cuts at: 1493 2759 3217 3443 3595 5704 1493
Size: 1266 458 226 152 2109 1697
Fragments arranged by size:
2109 1697 1266 458 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4462 160
Size: 872 3430 1606
Fragments arranged by size:
3430 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
AvaI C'yCGr_G
Cuts at: 1870 1943 2857 3233 1870
Size: 73 914 376 4545
Fragments arranged by size:
4545 914 376 73
BamHI G'GATC_C
Cuts at: 3238 3605 3238
Size: 367 5541
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2510 2937 3229 4684 618
Size: 325 217 627 723 427 292 1455 1842
Fragments arranged by size:
1842 1455 723 627 427 325 292 217
BanII G_rGCy'C
Cuts at: 1391 2241 2866 2970 3227 1391
Size: 850 625 104 257 4072
Fragments arranged by size:
4072 850 625 257 104
BbeI G_GCGC'C
Cuts at: 2514 2941 2514
Size: 427 5481
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 2725 928
Size: 971 699 127 4111
Fragments arranged by size:
4111 971 699 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2801 2835 4099 4133 1903
Size: 34 864 34 1264 34 3678
Fragments arranged by size:
3678 1264 864 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3800 5010 3800
Size: 1210 4698
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5908
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2604 4519 136
Size: 107 122 71 631 605 932 1915 1525
Fragments arranged by size:
1915 1525 932 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5903 5903
Size: 5908
BlpI GC'TnA_GC
Cuts at: 2272 3325 2272
Size: 1053 4855
Bme1580I G_kGCm'C
Cuts at: 1790 2241 2540 2866 3126 3171 3969 5125 1790
Size: 451 299 326 260 45 798 1156 2573
Fragments arranged by size:
2573 1156 798 451 326 299 260 45
BmgBI CAC'GTC
Cuts at: 1318 2254 2701 1318
Size: 936 447 4525
Fragments arranged by size:
4525 936 447
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3639 4597 442
Size: 416 53 83 399 24 2222 958 1753
Fragments arranged by size:
2222 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2336 2984 3026 3182 4568 1239
Size: 914 183 648 42 156 1386 2579
Fragments arranged by size:
2579 1386 914 648 183 156 42
Bpu10I CC'TnA_GC
Cuts at: 3141 3141
Size: 5908
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 2657 4033 4898 5196 2639
Size: 18 1376 865 298 3351
Fragments arranged by size:
3351 1376 865 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4571 882
Size: 617 3072 2219
Fragments arranged by size:
3072 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3699 493
Size: 711 2495 2702
Fragments arranged by size:
2702 2495 711
BsaBI GATnn'nnATC
Cuts at: 5902 5902
Size: 5908
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2511 2730 2938 2985
Size: 53 83 186 936 978 219 208 47
Cuts at: 2985 3715 4097 4732 275
Size: 730 382 635 1451
Fragments arranged by size:
1451 978 936 730 635 382 219 208
186 83 53 47
BsaWI w'CCGG_w
Cuts at: 2031 4341 4739 5013 5160 5571 2031
Size: 2310 398 274 147 411 2368
Fragments arranged by size:
2368 2310 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 1178
Size: 30 165 30 906 30 4747
Fragments arranged by size:
4747 906 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 1186
Size: 1384 4524
BseYI C'CCAG_C
Cuts at: 2533 3013 5111 5607 2533
Size: 480 2098 496 2834
Fragments arranged by size:
2834 2098 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2413 2927 3041 1163
Size: 573 677 514 114 4030
Fragments arranged by size:
4030 677 573 514 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2449 2979 3358 4119 5147 1540
Size: 275 186 448 530 379 761 1028 2301
Fragments arranged by size:
2301 1028 761 530 448 379 275 186
BsiHKAI G_wGCw'C
Cuts at: 2970 3126 3171 3207 3969 4054 5125 2970
Size: 156 45 36 762 85 1071 3753
Fragments arranged by size:
3753 1071 762 156 85 45 36
BsmI GAATG_Cn'
Cuts at: 3419 3512 3419
Size: 93 5815
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2540 2866 2970 3126 3171 3207
Size: 399 451 299 326 104 156 45 36
Cuts at: 3207 3227 3969 4054 5125 1391
Size: 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 399 326 299 156
104 85 45 36 20
BspEI T'CCGG_A
Cuts at: 5571 5571
Size: 5908
BspHI T'CATG_A
Cuts at: 3797 5527 3797
Size: 1730 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2418 2760 3191 3263 844
Size: 1574 342 431 72 3489
Fragments arranged by size:
3489 1574 431 342 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2485 3795 1394
Size: 429 662 1310 3507
Fragments arranged by size:
3507 1310 662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4403 4577 65
Size: 1183 3155 174 1396
Fragments arranged by size:
3155 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2772 2921 4552 4739 1700
Size: 110 962 149 1631 187 2869
Fragments arranged by size:
2869 1631 962 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5908
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5908
BssSI C'ACGA_G
Cuts at: 2060 3035 3116 3968 4980 2060
Size: 975 81 852 1012 2988
Fragments arranged by size:
2988 1012 975 852 81
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3130 794
Size: 2336 3572
BstBI TT'CG_AA
Cuts at: 4712 4712
Size: 5908
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5908
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 3118 2224
Size: 894 5014
BstYI r'GATC_y
Cuts at: 1972 2661 3238 3605 3990 4007 5459 5545 5557
Size: 689 577 367 385 17 1452 86 12
Cuts at: 5557 5903 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 689 577 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2223 2493 3027 513
Size: 552 1158 270 534 3394
Fragments arranged by size:
3394 1158 552 534 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2415 2817 487
Size: 683 56 1189 402 3578
Fragments arranged by size:
3578 1189 683 402 56
BtsI GCAGTG_nn'
Cuts at: 2350 2624 3424 4218 4238 2350
Size: 274 800 794 20 4020
Fragments arranged by size:
4020 800 794 274 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5908
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5873
DraI TTT'AAA
Cuts at: 3212 3212
Size: 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2729 2735 4915 809
Size: 507 1413 6 2180 1802
Fragments arranged by size:
2180 1802 1413 507 6
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2919 3355
Size: 54 1425 50 275 18 234 855 436
Cuts at: 3355 4244 5873 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 855 436 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3355 1537
Size: 275 1543 4090
Fragments arranged by size:
4090 1543 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 2531 3838 1353
Size: 992 186 1307 3423
Fragments arranged by size:
3423 1307 992 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2109 2453 2877 4495 4869 5015
Size: 1064 138 520 344 424 1618 374 146
Cuts at: 5015 387
Size: 1280
Fragments arranged by size:
1618 1280 1064 520 424 374 344 146
138
EcoICRI GAG'CTC
Cuts at: 2968 2968
Size: 5908
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2336 2984 3026 3182 3971 4568
Size: 393 521 183 648 42 156 789 597
Cuts at: 4568 5355 5623 1239
Size: 787 268 1524
Fragments arranged by size:
1524 789 787 648 597 521 393 268
183 156 42
EcoO109I rG'GnC_Cy
Cuts at: 2237 2238 2311 2862 2863 2237
Size: 1 73 551 1 5282
Fragments arranged by size:
5282 551 73 1 1
EcoRI G'AATT_C
Cuts at: 3217 3595 3217
Size: 378 5530
FspI TGC'GCA
Cuts at: 2519 4414 2519
Size: 1895 4013
FspAI rTGC'GCAy
Cuts at: 2519 2519
Size: 5908
HaeII r_GCGC'y
Cuts at: 1678 2514 2941 5055 1678
Size: 836 427 2114 2531
Fragments arranged by size:
2531 2114 836 427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 4549 4581 4623
Size: 32 135 32 902 32 2043 32 42
Cuts at: 4623 4655 1373
Size: 32 2626
Fragments arranged by size:
2626 2043 902 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2740 3252 3498 669
Size: 988 281 802 512 246 3079
Fragments arranged by size:
3079 988 802 512 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5908
HpaI GTT'AAC
Cuts at: 3498 3498
Size: 5908
KasI G'GCGC_C
Cuts at: 2510 2937 2510
Size: 427 5481
KpnI G_GTAC'C
Cuts at: 3233 3233
Size: 5908
MfeI C'AATT_G
Cuts at: 3507 3507
Size: 5908
MluI A'CGCG_T
Cuts at: 5784 5784
Size: 5908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 5022 5206 1305
Size: 799 2918 184 2007
Fragments arranged by size:
2918 2007 799 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4086
Fragments arranged by size:
4086 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 3116 3866 4225 4384 518
Size: 648 1056 894 750 359 159 2042
Fragments arranged by size:
2042 1056 894 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2495 2578 3324 4001 5149 5394 1606
Size: 561 328 83 746 677 1148 245 2120
Fragments arranged by size:
2120 1148 746 677 561 328 245 83
NaeI GCC'GGC
Cuts at: 2923 2923
Size: 5908
NarI GG'CG_CC
Cuts at: 2511 2938 2511
Size: 427 5481
NcoI C'CATG_G
Cuts at: 513 1065 2223 513
Size: 552 1158 4198
Fragments arranged by size:
4198 1158 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5908
NgoMIV G'CCGG_C
Cuts at: 2921 2921
Size: 5908
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5908
NotI GC'GGCC_GC
Cuts at: 3355 3355
Size: 5908
NspI r_CATG'y
Cuts at: 1132 1229 3001 3266 4811 1132
Size: 97 1772 265 1545 2229
Fragments arranged by size:
2229 1772 1545 265 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5908
PciI A'CATG_T
Cuts at: 4807 4807
Size: 5908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2314 1108
Size: 1206 4702
PfoI T'CCnGG_A
Cuts at: 2313 3062 2313
Size: 749 5159
PmeI GTTT'AAAC
Cuts at: 3212 3212
Size: 5908
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2309 2341 3977 4009 5490 5522 2309
Size: 32 1636 32 1481 32 2695
Fragments arranged by size:
2695 1636 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2311 2311
Size: 5908
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5908
PsiI TTA'TAA
Cuts at: 3478 3478
Size: 5908
PspOMI G'GGCC_C
Cuts at: 2237 2862 2237
Size: 625 5283
PstI C_TGCA'G
Cuts at: 830 2112 2619 2814 3260 830
Size: 1282 507 195 446 3478
Fragments arranged by size:
3478 1282 507 446 195
PvuI CG_AT'CG
Cuts at: 2001 2979 2001
Size: 978 4930
PvuII CAG'CTG
Cuts at: 2167 2578 2167
Size: 411 5497
SacI G_AGCT'C
Cuts at: 2970 2970
Size: 5908
SacII CC_GC'GG
Cuts at: 2496 2496
Size: 5908
SalI G'TCGA_C
Cuts at: 1936 3250 1936
Size: 1314 4594
SbfI CC_TGCA'GG
Cuts at: 3260 3260
Size: 5908
ScaI AGT'ACT
Cuts at: 4156 5868 4156
Size: 1712 4196
SfcI C'TryA_G
Cuts at: 826 1045 2108 2615 2810 3256 4391 5072 5263
Size: 219 1063 507 195 446 1135 681 191
Cuts at: 5263 826
Size: 1471
Fragments arranged by size:
1471 1135 1063 681 507 446 219 195
191
SfoI GGC'GCC
Cuts at: 2512 2939 2512
Size: 427 5481
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5908
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5908
SmaI CCC'GGG
Cuts at: 1872 2859 3235 1872
Size: 987 376 4545
Fragments arranged by size:
4545 987 376
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 2672 4012 4913 5175 820
Size: 197 926 711 18 1340 901 262 1553
Fragments arranged by size:
1553 1340 926 901 711 262 197 18
SnaBI TAC'GTA
Cuts at: 493 3699 493
Size: 3206 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5908
SphI G_CATG'C
Cuts at: 1132 3266 1132
Size: 2134 3774
SspI AAT'ATT
Cuts at: 5 52 3832 5
Size: 47 3780 2081
Fragments arranged by size:
3780 2081 47
StuI AGG'CCT
Cuts at: 1517 2246 1517
Size: 729 5179
StyI C'CwwG_G
Cuts at: 513 1065 2223 3347 513
Size: 552 1158 1124 3074
Fragments arranged by size:
3074 1158 1124 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 2419 3935 3952 4105 4290 4735
Size: 1255 560 279 1516 17 153 185 445
Cuts at: 4735 325
Size: 1498
Fragments arranged by size:
1516 1498 1255 560 445 279 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4154 5866 96
Size: 274 80 33 51 1443 2177 1712 138
Fragments arranged by size:
2177 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2154 2186 5868 5900 2154
Size: 32 3682 32 2162
Fragments arranged by size:
3682 2162 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2984 1316
Size: 1668 4240
XbaI T'CTAG_A
Cuts at: 3244 3244
Size: 5908
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2156 3160 2156
Size: 1004 4904
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5908
XmaI C'CCGG_G
Cuts at: 1870 2857 3233 1870
Size: 987 376 4545
Fragments arranged by size:
4545 987 376
XmnI GAAnn'nnTTC
Cuts at: 4037 4037
Size: 5908
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2731 2986 3716 276
Size: 53 83 186 936 1197 255 730 2468
Fragments arranged by size:
2468 1197 936 730 255 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SrfI SwaI