(Circular) (Six-base) MAPSORT of: pFN21AE0123.seq Check: 1572 from: 1 to: 5572 pFN21AE0123 5572 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 1937 Size: 978 4594 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2885 3635 5019 5287 1632 Size: 1253 750 1384 268 1917 Fragments arranged by size: 1917 1384 1253 750 268 AfeI AGC'GCT Cuts at: 2077 2077 Size: 5572 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 4471 5448 1203 Size: 3268 977 1327 Fragments arranged by size: 3268 1327 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AlwNI CAG_nnn'CTG Cuts at: 2788 3315 4887 2788 Size: 527 1572 3473 Fragments arranged by size: 3473 1572 527 ApaI G_GGCC'C Cuts at: 2767 2797 2767 Size: 30 5542 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2115 2444 2568 2897 1870 Size: 73 172 329 124 329 4545 Fragments arranged by size: 4545 329 329 172 124 73 BamHI G'GATC_C Cuts at: 2479 2902 3269 2479 Size: 423 367 4782 Fragments arranged by size: 4782 423 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2810 2893 4348 618 Size: 325 217 627 1023 83 1455 1842 Fragments arranged by size: 1842 1455 1023 627 325 217 83 BanII G_rGCy'C Cuts at: 1391 2445 2767 2797 2891 1391 Size: 1054 322 30 94 4072 Fragments arranged by size: 4072 1054 322 94 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4601 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2293 3464 4674 2293 Size: 1171 1210 3191 Fragments arranged by size: 3191 1210 1171 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4183 136 Size: 107 122 71 631 605 2511 1525 Fragments arranged by size: 2511 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 2767 2797 3633 4789 1790 Size: 977 30 836 1156 2573 Fragments arranged by size: 2573 1156 977 836 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5572 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2741 4232 1239 Size: 855 647 1491 2579 Fragments arranged by size: 2579 1491 855 647 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2630 3697 4562 4860 2630 Size: 1067 865 298 3342 Fragments arranged by size: 3342 1067 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4235 882 Size: 617 2736 2219 Fragments arranged by size: 2736 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3363 493 Size: 711 2159 2702 Fragments arranged by size: 2702 2159 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2041 3379 3761 4396 Size: 53 83 186 936 508 1338 382 635 Cuts at: 4396 275 Size: 1451 Fragments arranged by size: 1451 1338 936 635 508 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4005 4403 4677 4824 5235 4005 Size: 398 274 147 411 4342 Fragments arranged by size: 4342 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2031 2061 2714 2744 1178 Size: 30 165 30 628 30 653 30 4006 Fragments arranged by size: 4006 653 628 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2453 2738 2741 1186 Size: 840 427 285 3 4017 Fragments arranged by size: 4017 840 427 285 3 BseYI C'CCAG_C Cuts at: 2072 2249 2292 2549 2640 4775 5271 2072 Size: 177 43 257 91 2135 496 2373 Fragments arranged by size: 2373 2135 496 257 177 91 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4999 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2060 3022 3783 4811 1540 Size: 275 186 59 962 761 1028 2301 Fragments arranged by size: 2301 1028 962 761 275 186 59 BsiHKAI G_wGCw'C Cuts at: 2580 2843 3633 3718 4789 2580 Size: 263 790 85 1071 3363 Fragments arranged by size: 3363 1071 790 263 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 BsmBI CGTCTCn'nnnn_ Cuts at: 2051 2112 2051 Size: 61 5511 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2445 2580 2767 2797 2843 2891 3633 Size: 399 655 135 187 30 46 48 742 Cuts at: 3633 3718 4789 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 655 399 187 135 85 48 46 30 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2688 2927 844 Size: 1844 239 3489 Fragments arranged by size: 3489 1844 239 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2265 4067 4241 65 Size: 1183 1017 1802 174 1396 Fragments arranged by size: 1802 1396 1183 1017 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2807 4216 4403 1700 Size: 110 249 748 1409 187 2869 Fragments arranged by size: 2869 1409 748 249 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 2704 2788 794 Size: 1910 84 3578 Fragments arranged by size: 3578 1910 84 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2542 2272 Size: 270 5302 BstYI r'GATC_y Cuts at: 1972 2479 2736 2902 3269 3654 3671 5123 5209 Size: 507 257 166 367 385 17 1452 86 Cuts at: 5209 5221 5567 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 507 385 367 346 257 166 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2652 513 Size: 552 1587 3433 Fragments arranged by size: 3433 1587 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4833 Fragments arranged by size: 4833 683 56 BtsI GCAGTG_nn' Cuts at: 2277 3088 3882 3902 2277 Size: 811 794 20 3947 Fragments arranged by size: 3947 811 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DraIII CAC_nnn'GTG Cuts at: 2177 2337 2177 Size: 160 5412 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2057 2262 3019 Size: 54 1425 50 275 18 227 205 757 Cuts at: 3019 3908 5537 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 757 275 227 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2057 3019 1537 Size: 275 245 962 4090 Fragments arranged by size: 4090 962 275 245 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 2822 3502 1353 Size: 785 684 680 3423 Fragments arranged by size: 3423 785 684 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2741 2885 3635 4232 5019 5287 Size: 393 462 647 144 750 597 787 268 Cuts at: 5287 1239 Size: 1524 Fragments arranged by size: 1524 787 750 647 597 462 393 268 144 EcoNI CCTnn'n_nnAGG Cuts at: 2723 2867 2723 Size: 144 5428 EcoO109I rG'GnC_Cy Cuts at: 2257 2546 2257 Size: 289 5283 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 2079 4719 1678 Size: 401 2640 2531 Fragments arranged by size: 2640 2531 401 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 2137 2169 2538 Size: 32 135 32 459 32 74 32 369 Cuts at: 2538 2570 4213 4245 4287 4319 1373 Size: 32 1643 32 42 32 2626 Fragments arranged by size: 2626 1643 459 369 135 74 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3162 669 Size: 988 281 978 246 3079 Fragments arranged by size: 3079 988 978 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4686 4870 1305 Size: 3381 184 2007 Fragments arranged by size: 3381 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 10 Size: 54 1425 343 432 3318 Fragments arranged by size: 3318 1425 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2772 3530 3889 4048 518 Size: 648 1104 502 758 359 159 2042 Fragments arranged by size: 2042 1104 758 648 502 359 159 MspA1I CmG'CkG Cuts at: 1606 2383 2988 3665 4813 5058 1606 Size: 777 605 677 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 605 245 NaeI GCC'GGC Cuts at: 2061 2809 2061 Size: 748 4824 NcoI C'CATG_G Cuts at: 513 1065 2652 513 Size: 552 1587 3433 Fragments arranged by size: 3433 1587 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NgoMIV G'CCGG_C Cuts at: 2059 2807 2059 Size: 748 4824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2930 4475 1132 Size: 97 1701 1545 2229 Fragments arranged by size: 2229 1701 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5572 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5572 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2546 2546 Size: 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PspOMI G'GGCC_C Cuts at: 2763 2793 2763 Size: 30 5542 PstI C_TGCA'G Cuts at: 830 2784 2924 830 Size: 1954 140 3478 Fragments arranged by size: 3478 1954 140 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 PvuII CAG'CTG Cuts at: 2383 2383 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SanDI GG'GwC_CC Cuts at: 2546 2546 Size: 5572 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 2312 3820 5532 2312 Size: 1508 1712 2352 Fragments arranged by size: 2352 1712 1508 SfcI C'TryA_G Cuts at: 826 1045 2428 2780 2920 4055 4736 4927 826 Size: 219 1383 352 140 1135 681 191 1471 Fragments arranged by size: 1471 1383 1135 681 352 219 191 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2117 2899 1872 Size: 245 782 4545 Fragments arranged by size: 4545 782 245 SmlI C'TyrA_G Cuts at: 820 1017 1943 2645 3676 4577 4839 820 Size: 197 926 702 1031 901 262 1553 Fragments arranged by size: 1553 1031 926 901 702 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SrfI GCCC'GGGC Cuts at: 2117 2117 Size: 5572 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 2652 2664 3011 513 Size: 552 1587 12 347 3074 Fragments arranged by size: 3074 1587 552 347 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3599 3616 3769 3954 4399 325 Size: 1255 2019 17 153 185 445 1498 Fragments arranged by size: 2019 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 3818 5530 Size: 274 80 33 51 1443 333 1508 1712 Cuts at: 5530 96 Size: 138 Fragments arranged by size: 1712 1508 1443 333 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5532 5564 5532 Size: 32 5540 Tth111I GACn'n_nGTC Cuts at: 1316 2043 1316 Size: 727 4845 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2490 2539 2257 Size: 233 49 5290 Fragments arranged by size: 5290 233 49 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2115 2897 1870 Size: 245 782 4545 Fragments arranged by size: 4545 782 245 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SwaI