(Circular) (Six-base) MAPSORT of: pFN21AE0484.seq Check: 9367 from: 1 to: 5383 pFN21AE0484 5383 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3193 278 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2726 1937 Size: 789 4594 Acc65I G'GTAC_C Cuts at: 2337 2704 2337 Size: 367 5016 AclI AA'CG_TT Cuts at: 1563 3510 3883 1563 Size: 1947 373 3063 Fragments arranged by size: 3063 1947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2547 3446 4830 5098 1632 Size: 915 899 1384 268 1917 Fragments arranged by size: 1917 1384 915 899 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5186 AflIII A'CryG_T Cuts at: 1203 4282 5259 1203 Size: 3079 977 1327 Fragments arranged by size: 3079 1327 977 AgeI A'CCGG_T Cuts at: 4214 4214 Size: 5383 AhdI GACnn_n'nnGTC Cuts at: 2249 2512 4112 2249 Size: 263 1600 3520 Fragments arranged by size: 3520 1600 263 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5383 AlwNI CAG_nnn'CTG Cuts at: 2386 3126 4698 2386 Size: 740 1572 3071 Fragments arranged by size: 3071 1572 740 ApaLI G'TGCA_C Cuts at: 3440 4596 3440 Size: 1156 4227 ApoI r'AATT_y Cuts at: 1493 2211 2692 2918 3070 5179 1493 Size: 718 481 226 152 2109 1697 Fragments arranged by size: 2109 1697 718 481 226 152 AseI AT'TA_AT Cuts at: 160 1032 3937 160 Size: 872 2905 1606 Fragments arranged by size: 2905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 AvaI C'yCGr_G Cuts at: 1870 1943 2708 1870 Size: 73 765 4545 Fragments arranged by size: 4545 765 73 BamHI G'GATC_C Cuts at: 2713 3080 2713 Size: 367 5016 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2337 2704 4159 618 Size: 325 217 627 550 367 1455 1842 Fragments arranged by size: 1842 1455 627 550 367 325 217 BanII G_rGCy'C Cuts at: 1391 2394 2702 1391 Size: 1003 308 4072 Fragments arranged by size: 4072 1003 308 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 928 Size: 971 636 3776 Fragments arranged by size: 3776 971 636 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3574 3608 1903 Size: 34 1637 34 3678 Fragments arranged by size: 3678 1637 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4485 3275 Size: 1210 4173 BclI T'GATC_A Cuts at: 1548 1548 Size: 5383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3994 136 Size: 107 122 71 631 605 2322 1525 Fragments arranged by size: 2322 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2550 5378 2550 Size: 2828 2555 BlpI GC'TnA_GC Cuts at: 2800 2800 Size: 5383 Bme1580I G_kGCm'C Cuts at: 1790 3444 4600 1790 Size: 1654 1156 2573 Fragments arranged by size: 2573 1654 1156 BmgBI CAC'GTC Cuts at: 1318 2026 1318 Size: 708 4675 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3114 4072 442 Size: 416 53 83 399 24 1697 958 1753 Fragments arranged by size: 1753 1697 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2408 4043 1239 Size: 1112 57 1635 2579 Fragments arranged by size: 2579 1635 1112 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3508 4373 4671 3508 Size: 865 298 4220 Fragments arranged by size: 4220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4046 882 Size: 617 2547 2219 Fragments arranged by size: 2547 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3174 493 Size: 711 1970 2702 Fragments arranged by size: 2702 1970 711 BsaBI GATnn'nnATC Cuts at: 5377 5377 Size: 5383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3190 3572 4207 275 Size: 53 83 186 936 1657 382 635 1451 Fragments arranged by size: 1657 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3816 4214 4488 4635 5046 3816 Size: 398 274 147 411 4153 Fragments arranged by size: 4153 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2349 2379 1178 Size: 30 165 30 946 30 4182 Fragments arranged by size: 4182 946 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5383 BseYI C'CCAG_C Cuts at: 2616 4586 5082 2616 Size: 1970 496 2917 Fragments arranged by size: 2917 1970 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2468 1163 Size: 573 732 4078 Fragments arranged by size: 4078 732 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2833 3594 4622 1540 Size: 275 186 832 761 1028 2301 Fragments arranged by size: 2301 1028 832 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3444 3529 4600 3444 Size: 85 1071 4227 Fragments arranged by size: 4227 1071 85 BsmI GAATG_Cn' Cuts at: 2894 2987 2894 Size: 93 5290 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2702 3444 3529 4600 1391 Size: 399 604 308 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 308 85 BspEI T'CCGG_A Cuts at: 5046 5046 Size: 5383 BspHI T'CATG_A Cuts at: 3272 5002 3272 Size: 1730 3653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 2738 844 Size: 1685 209 3489 Fragments arranged by size: 3489 1685 209 BsrBI CCG'CTC Cuts at: 1394 1823 3270 1394 Size: 429 1447 3507 Fragments arranged by size: 3507 1447 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3878 4052 65 Size: 1183 2630 174 1396 Fragments arranged by size: 2630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2598 4027 4214 1700 Size: 110 788 1429 187 2869 Fragments arranged by size: 2869 1429 788 187 110 BsrGI T'GTAC_A Cuts at: 96 2466 96 Size: 2370 3013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5383 BssSI C'ACGA_G Cuts at: 3443 4455 3443 Size: 1012 4371 BstAPI GCAn_nnn'nTGC Cuts at: 794 2626 794 Size: 1832 3551 BstBI TT'CG_AA Cuts at: 4187 4187 Size: 5383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5383 BstXI CCAn_nnnn'nTGG Cuts at: 2590 2590 Size: 5383 BstYI r'GATC_y Cuts at: 1972 2550 2605 2713 3080 3465 3482 4934 5020 Size: 578 55 108 367 385 17 1452 86 Cuts at: 5020 5032 5378 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 578 385 367 346 108 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3892 Fragments arranged by size: 3892 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2031 487 Size: 683 56 805 3839 Fragments arranged by size: 3839 805 683 56 BtsI GCAGTG_nn' Cuts at: 2451 2899 3693 3713 2451 Size: 448 794 20 4121 Fragments arranged by size: 4121 794 448 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5383 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5348 DraI TTT'AAA Cuts at: 2687 2687 Size: 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4390 809 Size: 507 3074 1802 Fragments arranged by size: 3074 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2830 3719 5348 Size: 54 1425 50 275 18 1000 889 1629 Cuts at: 5348 8 Size: 43 Fragments arranged by size: 1629 1425 1000 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2830 1537 Size: 275 1018 4090 Fragments arranged by size: 4090 1018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 3313 1353 Size: 753 1207 3423 Fragments arranged by size: 3423 1207 753 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3970 4344 4490 387 Size: 1064 138 2381 374 146 1280 Fragments arranged by size: 2381 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2351 2408 2547 3446 4043 4830 5098 Size: 393 719 57 139 899 597 787 268 Cuts at: 5098 1239 Size: 1524 Fragments arranged by size: 1524 899 787 719 597 393 268 139 57 EcoRI G'AATT_C Cuts at: 2692 3070 2692 Size: 378 5005 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2153 2153 Size: 5383 FspI TGC'GCA Cuts at: 3889 3889 Size: 5383 HaeII r_GCGC'y Cuts at: 1678 4530 1678 Size: 2852 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2235 2267 2349 2381 2498 Size: 32 135 32 663 32 82 32 117 Cuts at: 2498 2530 2535 2567 4024 4056 4098 4130 1373 Size: 32 5 32 1457 32 42 32 2626 Fragments arranged by size: 2626 1457 663 135 117 82 42 32 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 2727 2973 669 Size: 988 281 789 246 3079 Fragments arranged by size: 3079 988 789 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5383 HpaI GTT'AAC Cuts at: 2973 2973 Size: 5383 KpnI G_GTAC'C Cuts at: 2341 2708 2341 Size: 367 5016 MfeI C'AATT_G Cuts at: 2982 2982 Size: 5383 MluI A'CGCG_T Cuts at: 5259 5259 Size: 5383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 4497 4681 1305 Size: 1302 1890 184 2007 Fragments arranged by size: 2007 1890 1302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3561 Fragments arranged by size: 3561 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3341 3700 3859 518 Size: 648 2175 359 159 2042 Fragments arranged by size: 2175 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2799 3476 4624 4869 1606 Size: 1193 677 1148 245 2120 Fragments arranged by size: 2120 1193 1148 677 245 NaeI GCC'GGC Cuts at: 2600 2600 Size: 5383 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3892 Fragments arranged by size: 3892 939 552 NdeI CA'TA_TG Cuts at: 387 2046 387 Size: 1659 3724 NgoMIV G'CCGG_C Cuts at: 2598 2598 Size: 5383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5383 NotI GC'GGCC_GC Cuts at: 2830 2830 Size: 5383 NspI r_CATG'y Cuts at: 1132 1229 2741 4286 1132 Size: 97 1512 1545 2229 Fragments arranged by size: 2229 1545 1512 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5383 PciI A'CATG_T Cuts at: 4282 4282 Size: 5383 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 1108 Size: 1278 4105 PmeI GTTT'AAAC Cuts at: 2687 2687 Size: 5383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3452 3484 4965 4997 3452 Size: 32 1481 32 3838 Fragments arranged by size: 3838 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5383 PsiI TTA'TAA Cuts at: 2953 2953 Size: 5383 PstI C_TGCA'G Cuts at: 830 2446 2735 830 Size: 1616 289 3478 Fragments arranged by size: 3478 1616 289 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5383 SalI G'TCGA_C Cuts at: 1936 2725 1936 Size: 789 4594 SbfI CC_TGCA'GG Cuts at: 2735 2735 Size: 5383 ScaI AGT'ACT Cuts at: 3631 5343 3631 Size: 1712 3671 SexAI A'CCwGG_T Cuts at: 2333 2333 Size: 5383 SfcI C'TryA_G Cuts at: 826 1045 2442 2731 3866 4547 4738 826 Size: 219 1397 289 1135 681 191 1471 Fragments arranged by size: 1471 1397 1135 681 289 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5383 SmaI CCC'GGG Cuts at: 1872 2710 1872 Size: 838 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3487 4388 4650 820 Size: 197 926 1544 901 262 1553 Fragments arranged by size: 1553 1544 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3174 493 Size: 2681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5383 SphI G_CATG'C Cuts at: 1132 2741 1132 Size: 1609 3774 SspI AAT'ATT Cuts at: 5 52 2171 3307 5 Size: 47 2119 1136 2081 Fragments arranged by size: 2119 2081 1136 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5383 StyI C'CwwG_G Cuts at: 513 1065 2004 2822 513 Size: 552 939 818 3074 Fragments arranged by size: 3074 939 818 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3410 3427 3580 3765 4210 325 Size: 1255 1830 17 153 185 445 1498 Fragments arranged by size: 1830 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 3629 5341 Size: 274 80 33 51 1443 489 1163 1712 Cuts at: 5341 96 Size: 138 Fragments arranged by size: 1712 1443 1163 489 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5343 5375 5343 Size: 32 5351 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5383 XbaI T'CTAG_A Cuts at: 2719 2719 Size: 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5383 XmaI C'CCGG_G Cuts at: 1870 2708 1870 Size: 838 4545 XmnI GAAnn'nnTTC Cuts at: 3512 3512 Size: 5383 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3191 276 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI