################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AciI 285 GCGG CCGC(-3/-1) AfaI 93 GTAC GT^AC AlwI 135 GATCC GGATC(4/5) AlwNI 151 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 176 GTGCTC G[AT]GC[AT]^C BbsI 30 GAAGAC GAAGAC(2/6) BbvI 52 GCTGC GCAGC(8/12) BcnI 36 CCCGG CC^[GC]GG BfaI 140 CTAG C^TAG BmyI 176 GTGCTC G[AGT]GC[ACT]^C BpuAI 30 GAAGAC GAAGAC(2/6) BsiHKAI 176 GTGCTC G[AT]GC[AT]^C Bsp1286I 176 GTGCTC G[AGT]GC[ACT]^C BsrI 184 ACTGG ACTGG(1/-1) BssKI 36 CCCGG ^CC[ACGT]GG BstYI 134 AGATCC [GA]^GATC[CT] Cac8I 281 GCAAGC GC[ACGT]^[ACGT]GC CfoI 40 GCGC GCG^C Cfr13I 3 GGCCC G^G[ACGT]CC DpnII 135 GATC ^GATC EcoT14I 101 CCAAGG C^C[AT][AT]GG FauI 285 GCGGG CCCGC(4/6) Fnu4HI 52 GCTGC GC^[ACGT]GC HaeII 39 GGCGCT [GA]GCGC^[CT] HapII 37 CCGG C^CGG HhaI 40 GCGC GCG^C HinP1I 40 GCGC G^CGC HpaII 37 CCGG C^CGG HphI 45 GGTGA GGTGA(8/7) ItaI 52 GCTGC GC^[ACGT]GC MaeI 140 CTAG C^TAG MboI 135 GATC ^GATC MboII 30 GAAGA GAAGA(8/7) MflI 134 AGATCC [GA]^GATC[CT] MseI 209 TTAA T^TAA MslI 240 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 37 CCGG C^CGG NciI 36 CCCGG CC^[GC]GG NdeII 135 GATC ^GATC NlaIV 114 GGTTCC GG[ACGT]^[ACGT]CC PflMI 83 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 93 GTAC GT^AC Sau3AI 135 GATC ^GATC Sau96I 3 GGCCC G^G[ACGT]CC ScrFI 36 CCCGG CC^[ACGT]GG SfaNI 290 GCATC GCATC(5/9) StyI 101 CCAAGG C^C[AT][AT]GG Tru9I 209 TTAA T^TAA TseI 52 GCTGC G^C[AT]GC Tsp45I 58 GTGAC ^GT[GC]AC Tsp509I 8 AATT ^AATT TspRI 240 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 83 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 101 CCAAGGTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 134 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BalI 80 TGGCCA TGG^CCA 236 TGGCCA BsaJI 35 CCCCGG C^C[ACGT][ACGT]GG 101 CCAAGG BsiYI 36 CCCGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 83 CCACATTTTGG BslI 36 CCCGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 83 CCACATTTTGG EaeI 80 TGGCCA [CT]^GGCC[GA] 236 TGGCCA MaeIII 17 GTAAC ^GT[ACGT]AC 58 GTGAC MluNI 80 TGGCCA TGG^CCA 236 TGGCCA MscI 80 TGGCCA TGG^CCA 236 TGGCCA MwoI 281 GCAAGCGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 290 GCATCATTGGC TaqI 67 TCGA T^CGA 75 TCGA TthHB8I 67 TCGA T^CGA 75 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AluI DdeI HaeIII MnlI