(Circular) (Six-base) MAPSORT of: pFN21AB6890.seq Check: 5965 from: 1 to: 5311 pFN21AB6890 5311 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2306 2306 Size: 5311 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3121 278 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2654 1937 Size: 717 4594 Acc65I G'GTAC_C Cuts at: 2632 2632 Size: 5311 AclI AA'CG_TT Cuts at: 1563 3438 3811 1563 Size: 1875 373 3063 Fragments arranged by size: 3063 1875 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3374 4758 5026 1632 Size: 1742 1384 268 1917 Fragments arranged by size: 1917 1742 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5114 AflIII A'CryG_T Cuts at: 1203 2214 4210 5187 1203 Size: 1011 1996 977 1327 Fragments arranged by size: 1996 1327 1011 977 AgeI A'CCGG_T Cuts at: 4142 4142 Size: 5311 AhdI GACnn_n'nnGTC Cuts at: 4040 4040 Size: 5311 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5311 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5311 AlwNI CAG_nnn'CTG Cuts at: 2264 3054 4626 2264 Size: 790 1572 2949 Fragments arranged by size: 2949 1572 790 ApaI G_GGCC'C Cuts at: 2195 2195 Size: 5311 ApaLI G'TGCA_C Cuts at: 3368 4524 3368 Size: 1156 4155 ApoI r'AATT_y Cuts at: 1493 2159 2620 2846 2998 5107 1493 Size: 666 461 226 152 2109 1697 Fragments arranged by size: 2109 1697 666 461 226 152 AseI AT'TA_AT Cuts at: 160 1032 3865 160 Size: 872 2833 1606 Fragments arranged by size: 2833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2436 2636 1870 Size: 73 104 389 200 4545 Fragments arranged by size: 4545 389 200 104 73 BamHI G'GATC_C Cuts at: 2641 3008 2641 Size: 367 4944 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2632 4087 618 Size: 325 217 627 845 1455 1842 Fragments arranged by size: 1842 1455 845 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2195 2562 2630 1391 Size: 663 141 367 68 4072 Fragments arranged by size: 4072 663 367 141 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3502 3536 1903 Size: 34 1565 34 3678 Fragments arranged by size: 3678 1565 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3203 4413 3203 Size: 1210 4101 BclI T'GATC_A Cuts at: 1548 2164 1548 Size: 616 4695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3922 136 Size: 107 122 71 631 605 2250 1525 Fragments arranged by size: 2250 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5306 5306 Size: 5311 BlpI GC'TnA_GC Cuts at: 2728 2728 Size: 5311 Bme1580I G_kGCm'C Cuts at: 1790 2195 3372 4528 1790 Size: 405 1177 1156 2573 Fragments arranged by size: 2573 1177 1156 405 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5311 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2470 3042 4000 Size: 416 53 83 399 24 1053 572 958 Cuts at: 4000 442 Size: 1753 Fragments arranged by size: 1753 1053 958 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5311 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2138 2393 2504 3971 1239 Size: 821 78 255 111 1467 2579 Fragments arranged by size: 2579 1467 821 255 111 78 Bpu10I CC'TnA_GC Cuts at: 2364 2364 Size: 5311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3436 4301 4599 3436 Size: 865 298 4148 Fragments arranged by size: 4148 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2489 3974 882 Size: 617 990 1485 2219 Fragments arranged by size: 2219 1485 990 617 BsaAI yAC'GTr Cuts at: 493 1204 3102 493 Size: 711 1898 2702 Fragments arranged by size: 2702 1898 711 BsaBI GATnn'nnATC Cuts at: 5305 5305 Size: 5311 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2235 3118 3500 4135 Size: 53 83 186 936 702 883 382 635 Cuts at: 4135 275 Size: 1451 Fragments arranged by size: 1451 936 883 702 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2070 3744 4142 4416 4563 4974 2070 Size: 1674 398 274 147 411 2407 Fragments arranged by size: 2407 1674 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5086 Fragments arranged by size: 5086 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2573 1186 Size: 1387 3924 BseYI C'CCAG_C Cuts at: 2403 4514 5010 2403 Size: 2111 496 2704 Fragments arranged by size: 2704 2111 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2113 2284 2521 1163 Size: 573 377 171 237 3953 Fragments arranged by size: 3953 573 377 237 171 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2761 3522 4550 1540 Size: 275 186 760 761 1028 2301 Fragments arranged by size: 2301 1028 761 760 275 186 BsiHKAI G_wGCw'C Cuts at: 3372 3457 4528 3372 Size: 85 1071 4155 Fragments arranged by size: 4155 1071 85 BsmI GAATG_Cn' Cuts at: 2822 2915 2822 Size: 93 5218 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2195 2562 2630 3372 3457 4528 Size: 399 264 141 367 68 742 85 1071 Cuts at: 4528 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 367 264 141 85 68 BspEI T'CCGG_A Cuts at: 4974 4974 Size: 5311 BspHI T'CATG_A Cuts at: 3200 4930 3200 Size: 1730 3581 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2306 2666 844 Size: 1462 360 3489 Fragments arranged by size: 3489 1462 360 BsrBI CCG'CTC Cuts at: 1394 1823 3198 1394 Size: 429 1375 3507 Fragments arranged by size: 3507 1375 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3806 3980 65 Size: 1183 2558 174 1396 Fragments arranged by size: 2558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3955 4142 1700 Size: 110 2145 187 2869 Fragments arranged by size: 2869 2145 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5311 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5311 BssSI C'ACGA_G Cuts at: 3371 4383 3371 Size: 1012 4299 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5311 BstBI TT'CG_AA Cuts at: 4115 4115 Size: 5311 BstEII G'GTnAC_C Cuts at: 1184 2440 1184 Size: 1256 4055 BstYI r'GATC_y Cuts at: 1972 2169 2641 3008 3393 3410 4862 4948 4960 Size: 197 472 367 385 17 1452 86 12 Cuts at: 4960 5306 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 472 385 367 346 197 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2271 2308 2598 513 Size: 552 939 267 37 290 3226 Fragments arranged by size: 3226 939 552 290 267 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4572 Fragments arranged by size: 4572 683 56 BtsI GCAGTG_nn' Cuts at: 2101 2827 3621 3641 2101 Size: 726 794 20 3771 Fragments arranged by size: 3771 794 726 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5276 DraI TTT'AAA Cuts at: 2615 2615 Size: 5311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4318 809 Size: 507 3002 1802 Fragments arranged by size: 3002 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2758 3647 Size: 54 1425 50 275 18 177 751 889 Cuts at: 3647 5276 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 751 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2758 1537 Size: 275 946 4090 Fragments arranged by size: 4090 946 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3241 1353 Size: 1888 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 3898 4272 4418 387 Size: 1064 138 534 1775 374 146 1280 Fragments arranged by size: 1775 1280 1064 534 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2138 2393 2504 3374 3971 4758 Size: 393 428 78 255 111 870 597 787 Cuts at: 4758 5026 1239 Size: 268 1524 Fragments arranged by size: 1524 870 787 597 428 393 268 255 111 78 EcoO109I rG'GnC_Cy Cuts at: 2192 2503 2192 Size: 311 5000 EcoRI G'AATT_C Cuts at: 2620 2998 2620 Size: 378 4933 FspI TGC'GCA Cuts at: 3817 3817 Size: 5311 HaeII r_GCGC'y Cuts at: 1678 4458 1678 Size: 2780 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373 Size: 32 135 32 2380 32 42 32 2626 Fragments arranged by size: 2626 2380 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2344 2655 2901 669 Size: 988 281 406 311 246 3079 Fragments arranged by size: 3079 988 406 311 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5311 HpaI GTT'AAC Cuts at: 2901 2901 Size: 5311 KpnI G_GTAC'C Cuts at: 2636 2636 Size: 5311 MfeI C'AATT_G Cuts at: 2910 2910 Size: 5311 MluI A'CGCG_T Cuts at: 5187 5187 Size: 5311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4425 4609 1305 Size: 3120 184 2007 Fragments arranged by size: 3120 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3312 Fragments arranged by size: 3312 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 3269 3628 3787 518 Size: 648 1161 942 359 159 2042 Fragments arranged by size: 2042 1161 942 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2413 2727 3404 4552 4797 1606 Size: 807 314 677 1148 245 2120 Fragments arranged by size: 2120 1148 807 677 314 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2308 2598 513 Size: 552 939 304 290 3226 Fragments arranged by size: 3226 939 552 304 290 NdeI CA'TA_TG Cuts at: 387 387 Size: 5311 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5311 NotI GC'GGCC_GC Cuts at: 2758 2758 Size: 5311 NspI r_CATG'y Cuts at: 1132 1229 2218 2669 4214 1132 Size: 97 989 451 1545 2229 Fragments arranged by size: 2229 1545 989 451 97 PasI CC'CwG_GG Cuts at: 1348 2512 1348 Size: 1164 4147 PciI A'CATG_T Cuts at: 2214 4210 2214 Size: 1996 3315 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5311 PfoI T'CCnGG_A Cuts at: 2037 2037 Size: 5311 PmeI GTTT'AAAC Cuts at: 2615 2615 Size: 5311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3412 4893 4925 3380 Size: 32 1481 32 3766 Fragments arranged by size: 3766 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 2503 Size: 5311 PshAI GACnn'nnGTC Cuts at: 1613 2285 1613 Size: 672 4639 PsiI TTA'TAA Cuts at: 2881 2881 Size: 5311 PspOMI G'GGCC_C Cuts at: 2191 2191 Size: 5311 PstI C_TGCA'G Cuts at: 830 2663 830 Size: 1833 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5311 SalI G'TCGA_C Cuts at: 1936 2653 1936 Size: 717 4594 SbfI CC_TGCA'GG Cuts at: 2663 2663 Size: 5311 ScaI AGT'ACT Cuts at: 3559 5271 3559 Size: 1712 3599 SfcI C'TryA_G Cuts at: 826 1045 2659 3794 4475 4666 826 Size: 219 1614 1135 681 191 1471 Fragments arranged by size: 1614 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5311 SmaI CCC'GGG Cuts at: 1872 2438 2638 1872 Size: 566 200 4545 Fragments arranged by size: 4545 566 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 3415 4316 4578 820 Size: 197 926 1472 901 262 1553 Fragments arranged by size: 1553 1472 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3102 493 Size: 2609 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5311 SphI G_CATG'C Cuts at: 1132 2669 1132 Size: 1537 3774 SspI AAT'ATT Cuts at: 5 52 3235 5 Size: 47 3183 2081 Fragments arranged by size: 3183 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5311 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2308 2598 2750 513 Size: 552 939 6 298 290 152 3074 Fragments arranged by size: 3074 939 552 298 290 152 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2264 3338 3355 3508 3693 4138 325 Size: 1255 684 1074 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1074 684 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3557 5269 96 Size: 274 80 33 51 1443 1580 1712 138 Fragments arranged by size: 1712 1580 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5271 5303 5271 Size: 32 5279 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5311 XbaI T'CTAG_A Cuts at: 2647 2647 Size: 5311 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5311 XmaI C'CCGG_G Cuts at: 1870 2436 2636 1870 Size: 566 200 4545 Fragments arranged by size: 4545 566 200 XmnI GAAnn'nnTTC Cuts at: 3440 3440 Size: 5311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3119 276 Size: 53 83 186 936 1585 2468 Fragments arranged by size: 2468 1585 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI