(Circular) (Six-base) MAPSORT of: pFN21AE0376.seq Check: 1460 from: 1 to: 6391 pFN21AE0376 6391 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4201 278 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3734 1937 Size: 1797 4594 Acc65I G'GTAC_C Cuts at: 2202 3712 2202 Size: 1510 4881 AclI AA'CG_TT Cuts at: 1563 4518 4891 1563 Size: 2955 373 3063 Fragments arranged by size: 3063 2955 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2762 3393 3456 4454 5838 6106 1632 Size: 1130 631 63 998 1384 268 1917 Fragments arranged by size: 1917 1384 1130 998 631 268 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 5290 6267 1203 Size: 4087 977 1327 Fragments arranged by size: 4087 1327 977 AgeI A'CCGG_T Cuts at: 5222 5222 Size: 6391 AhdI GACnn_n'nnGTC Cuts at: 5120 5120 Size: 6391 AleI CACnn'nnGTG Cuts at: 1166 2954 3282 1166 Size: 1788 328 4275 Fragments arranged by size: 4275 1788 328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6391 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2772 2804 2772 Size: 32 6359 AlwNI CAG_nnn'CTG Cuts at: 4134 5706 4134 Size: 1572 4819 ApaLI G'TGCA_C Cuts at: 2167 4448 5604 2167 Size: 2281 1156 2954 Fragments arranged by size: 2954 2281 1156 ApoI r'AATT_y Cuts at: 1493 3362 3700 3926 4078 6187 1493 Size: 1869 338 226 152 2109 1697 Fragments arranged by size: 2109 1869 1697 338 226 152 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 3716 1870 Size: 73 1773 4545 Fragments arranged by size: 4545 1773 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2629 2662 2629 Size: 33 6358 BamHI G'GATC_C Cuts at: 3721 4088 3721 Size: 367 6024 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2202 2352 2431 3383 3712 Size: 325 217 627 415 150 79 952 329 Cuts at: 3712 5167 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 952 627 415 329 325 217 150 79 BanII G_rGCy'C Cuts at: 1391 2994 3138 3710 1391 Size: 1603 144 572 4072 Fragments arranged by size: 4072 1603 572 144 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 928 Size: 971 371 5049 Fragments arranged by size: 5049 971 371 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4582 4616 1903 Size: 34 2645 34 3678 Fragments arranged by size: 3678 2645 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3083 4283 5493 3083 Size: 1200 1210 3981 Fragments arranged by size: 3981 1210 1200 BclI T'GATC_A Cuts at: 1548 2383 3126 3522 1548 Size: 835 743 396 4417 Fragments arranged by size: 4417 835 743 396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5002 136 Size: 107 122 71 631 605 3330 1525 Fragments arranged by size: 3330 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6386 6386 Size: 6391 BlpI GC'TnA_GC Cuts at: 3808 3808 Size: 6391 Bme1580I G_kGCm'C Cuts at: 1790 2171 4452 5608 1790 Size: 381 2281 1156 2573 Fragments arranged by size: 2573 2281 1156 381 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4122 5080 442 Size: 416 53 83 399 24 2705 958 1753 Fragments arranged by size: 2705 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3008 5051 1239 Size: 1769 2043 2579 Fragments arranged by size: 2579 2043 1769 Bpu10I CC'TnA_GC Cuts at: 2492 2492 Size: 6391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3445 4516 5381 5679 3445 Size: 1071 865 298 4157 Fragments arranged by size: 4157 1071 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3459 5054 882 Size: 617 1960 1595 2219 Fragments arranged by size: 2219 1960 1595 617 BsaAI yAC'GTr Cuts at: 493 1204 2256 2812 4182 493 Size: 711 1052 556 1370 2702 Fragments arranged by size: 2702 1370 1052 711 556 BsaBI GATnn'nnATC Cuts at: 6385 6385 Size: 6391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3248 4198 4580 5215 Size: 53 83 186 936 1715 950 382 635 Cuts at: 5215 275 Size: 1451 Fragments arranged by size: 1715 1451 950 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3093 4824 5222 5496 5643 6054 3093 Size: 1731 398 274 147 411 3430 Fragments arranged by size: 3430 1731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2772 2802 1178 Size: 30 165 30 1369 30 4767 Fragments arranged by size: 4767 1369 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 2732 2780 3437 1186 Size: 1414 132 48 657 4140 Fragments arranged by size: 4140 1414 657 132 48 BseYI C'CCAG_C Cuts at: 2142 3013 3116 5594 6090 2142 Size: 871 103 2478 496 2443 Fragments arranged by size: 2478 2443 871 496 103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 2792 3281 1163 Size: 573 729 327 489 4273 Fragments arranged by size: 4273 729 573 489 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2200 3841 4602 5630 1540 Size: 275 186 199 1641 761 1028 2301 Fragments arranged by size: 2301 1641 1028 761 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2099 2171 4452 4537 5608 2099 Size: 72 2281 85 1071 2882 Fragments arranged by size: 2882 2281 1071 85 72 BsmI GAATG_Cn' Cuts at: 3902 3995 3902 Size: 93 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2099 2171 2994 3138 3710 4452 4537 Size: 399 309 72 823 144 572 742 85 Cuts at: 4537 5608 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 823 742 572 399 309 144 85 72 BspEI T'CCGG_A Cuts at: 6054 6054 Size: 6391 BspHI T'CATG_A Cuts at: 4280 6010 4280 Size: 1730 4661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2856 3746 844 Size: 2012 890 3489 Fragments arranged by size: 3489 2012 890 BsrBI CCG'CTC Cuts at: 1394 1823 2401 4278 1394 Size: 429 578 1877 3507 Fragments arranged by size: 3507 1877 578 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4886 5060 65 Size: 1183 3638 174 1396 Fragments arranged by size: 3638 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2195 2199 5035 5222 1700 Size: 110 385 4 2836 187 2869 Fragments arranged by size: 2869 2836 385 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 3263 4451 5463 3263 Size: 1188 1012 4191 Fragments arranged by size: 4191 1188 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6391 BstBI TT'CG_AA Cuts at: 5195 5195 Size: 6391 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6391 BstXI CCAn_nnnn'nTGG Cuts at: 3148 3284 3148 Size: 136 6255 BstYI r'GATC_y Cuts at: 1972 2508 3030 3186 3721 4088 4473 4490 5942 Size: 536 522 156 535 367 385 17 1452 Cuts at: 5942 6028 6040 6386 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 536 535 522 385 367 346 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2320 2407 2514 2582 3614 3681 513 Size: 552 1255 87 107 68 1032 67 3223 Fragments arranged by size: 3223 1255 1032 552 107 87 68 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5652 Fragments arranged by size: 5652 683 56 BtsI GCAGTG_nn' Cuts at: 3907 4701 4721 3907 Size: 794 20 5577 Fragments arranged by size: 5577 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6391 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6356 DraI TTT'AAA Cuts at: 3695 3695 Size: 6391 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5398 809 Size: 507 4082 1802 Fragments arranged by size: 4082 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2197 2940 3098 Size: 54 1425 50 275 18 367 743 158 Cuts at: 3098 3214 3838 4727 6356 8 Size: 116 624 889 1629 43 Fragments arranged by size: 1629 1425 889 743 624 367 275 158 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2197 3838 1537 Size: 275 385 1641 4090 Fragments arranged by size: 4090 1641 385 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3278 4321 1353 Size: 1925 1043 3423 Fragments arranged by size: 3423 1925 1043 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3195 4978 5352 5498 387 Size: 1064 138 1606 1783 374 146 1280 Fragments arranged by size: 1783 1606 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2762 3008 3393 3456 4454 5051 5838 Size: 393 1130 246 385 63 998 597 787 Cuts at: 5838 6106 1239 Size: 268 1524 Fragments arranged by size: 1524 1130 998 787 597 393 385 268 246 63 EcoNI CCTnn'n_nnAGG Cuts at: 2927 2927 Size: 6391 EcoO109I rG'GnC_Cy Cuts at: 3636 3636 Size: 6391 EcoRI G'AATT_C Cuts at: 3700 4078 3700 Size: 378 6013 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2611 2799 3486 2611 Size: 188 687 5516 Fragments arranged by size: 5516 687 188 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 2938 5538 1678 Size: 1260 2600 2531 Fragments arranged by size: 2600 2531 1260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5032 5064 5106 5138 1373 Size: 32 135 32 3460 32 42 32 2626 Fragments arranged by size: 3460 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3735 3981 669 Size: 988 281 1797 246 3079 Fragments arranged by size: 3079 1797 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6391 HpaI GTT'AAC Cuts at: 3981 3981 Size: 6391 KpnI G_GTAC'C Cuts at: 2206 3716 2206 Size: 1510 4881 MfeI C'AATT_G Cuts at: 3990 3990 Size: 6391 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2645 3185 3282 5505 5689 1305 Size: 1340 540 97 2223 184 2007 Fragments arranged by size: 2223 2007 1340 540 184 97 MscI TGG'CCA Cuts at: 10 64 1489 1832 2942 10 Size: 54 1425 343 1110 3459 Fragments arranged by size: 3459 1425 1110 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 2954 3282 4349 4708 4867 518 Size: 648 1512 276 328 1067 359 159 2042 Fragments arranged by size: 2042 1512 1067 648 359 328 276 159 MspA1I CmG'CkG Cuts at: 1606 2156 2487 2568 3013 3807 4484 5632 5877 Size: 550 331 81 445 794 677 1148 245 Cuts at: 5877 1606 Size: 2120 Fragments arranged by size: 2120 1148 794 677 550 445 331 245 81 NcoI C'CATG_G Cuts at: 513 1065 2407 2582 3614 3681 513 Size: 552 1342 175 1032 67 3223 Fragments arranged by size: 3223 1342 1032 552 175 67 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 3749 5294 1132 Size: 97 2520 1545 2229 Fragments arranged by size: 2520 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6391 PciI A'CATG_T Cuts at: 5290 5290 Size: 6391 PflMI CCAn_nnn'nTGG Cuts at: 1108 2953 1108 Size: 1845 4546 PfoI T'CCnGG_A Cuts at: 2913 2913 Size: 6391 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PmlI CAC'GTG Cuts at: 2256 2256 Size: 6391 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2772 2804 4460 4492 5973 6005 2772 Size: 32 1656 32 1481 32 3158 Fragments arranged by size: 3158 1656 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3636 3636 Size: 6391 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6391 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3017 3049 3017 Size: 32 6359 PstI C_TGCA'G Cuts at: 830 2316 3624 3743 830 Size: 1486 1308 119 3478 Fragments arranged by size: 3478 1486 1308 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 PvuII CAG'CTG Cuts at: 2156 3013 2156 Size: 857 5534 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 4639 6351 4639 Size: 1712 4679 SfcI C'TryA_G Cuts at: 826 1045 2312 3620 3739 4874 5555 5746 826 Size: 219 1267 1308 119 1135 681 191 1471 Fragments arranged by size: 1471 1308 1267 1135 681 219 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3718 1872 Size: 1846 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3460 4495 5396 5658 820 Size: 197 926 1517 1035 901 262 1553 Fragments arranged by size: 1553 1517 1035 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6391 SphI G_CATG'C Cuts at: 1132 3749 1132 Size: 2617 3774 SspI AAT'ATT Cuts at: 5 52 2107 4315 5 Size: 47 2055 2208 2081 Fragments arranged by size: 2208 2081 2055 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6391 StyI C'CwwG_G Cuts at: 513 1065 2053 2407 2582 2952 3614 3681 3830 Size: 552 988 354 175 370 662 67 149 Cuts at: 3830 513 Size: 3074 Fragments arranged by size: 3074 988 662 552 370 354 175 149 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3005 3642 4418 4435 4588 4773 5218 Size: 1255 1425 637 776 17 153 185 445 Cuts at: 5218 325 Size: 1498 Fragments arranged by size: 1498 1425 1255 776 637 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2958 4637 6349 Size: 274 80 33 51 1443 981 1679 1712 Cuts at: 6349 96 Size: 138 Fragments arranged by size: 1712 1679 1443 981 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6351 6383 6351 Size: 32 6359 Tth111I GACn'n_nGTC Cuts at: 1316 3166 1316 Size: 1850 4541 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2950 3145 2950 Size: 195 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3716 1870 Size: 1846 4545 XmnI GAAnn'nnTTC Cuts at: 2784 3406 3480 4520 2784 Size: 622 74 1040 4655 Fragments arranged by size: 4655 1040 622 74 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4199 276 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI