################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstF5I BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MroI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfcI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 318 CGCG CG^CG AlwI 240 GATCC GGATC(4/5) ApaI 45 GGGCCC GGGCC^C AspHI 401 GTGCTC G[AT]GC[AT]^C AviII 35 TGCGCA TGC^GCA BcnI 186 CCGGG CC^[GC]GG BglI 55 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 223 CTGGAG CTGGAG(16/14) BsgI 202 CTGCAC GTGCAG(16/14) BsiEI 53 CGGCCG CG[GA][CT]^CG BsiHKAI 401 GTGCTC G[AT]GC[AT]^C BsmBI 96 GAGACG CGTCTC(1/5) BsmFI 17 GGGAC GGGAC(10/14) BsrI 14 ACTGG ACTGG(1/-1) BssSI 404 CTCGTG CACGAG(-5/-1) BstEII 216 GGTGACC G^GT[ACGT]ACC BstPI 216 GGTGACC G^GT[ACGT]ACC BstUI 318 CGCG CG^CG CfoI 36 GCGC GCG^C EagI 53 CGGCCG C^GGCCG EclXI 53 CGGCCG C^GGCCG Eco52I 53 CGGCCG C^GGCCG EcoO65I 216 GGTGACC G^GT[ACGT]ACC FauI 375 GCGGG CCCGC(4/6) FspI 35 TGCGCA TGC^GCA HapII 186 CCGG C^CGG HhaI 36 GCGC GCG^C HinP1I 36 GCGC G^CGC HpaII 186 CCGG C^CGG MboII 106 GAAGA GAAGA(8/7) MslI 381 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 186 CCGG C^CGG MvnI 318 CGCG CG^CG NciI 186 CCGGG CC^[GC]GG NlaIII 208 CATG CATG^ PflMI 122 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 278 AGGACCT [GA]G^G[AT]CC[CT] SexAI 353 ACCAGGT A^CC[AT]GGT SfiI 54 GGCCGCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 371 TCGA T^CGA TthHB8I 371 TCGA T^CGA Van91I 122 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AluI 179 AGCT AG^CT 200 AGCT AvaII 183 GGACC G^G[AT]CC 279 GGACC BalI 138 TGGCCA TGG^CCA 284 TGGCCA BanII 45 GGGCCC G[GA]GC[CT]^C 416 GAGCCC BsaJI 59 CCCAGG C^C[ACGT][ACGT]GG 221 CCCTGG BseRI 101 GAGGAG GAGGAG(10/8) 196 GAGGAG BsrBI 330 GAGCGG CCGCTC(-3/-3) 373 GAGCGG BstXI 354 CCAGGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 380 CCATTGATGTGG DdeI 84 CTTAG C^T[ACGT]AG 412 CTGAG DpnII 240 GATC ^GATC 315 GATC MaeIII 25 GTGAC ^GT[ACGT]AC 217 GTGAC MboI 240 GATC ^GATC 315 GATC MluNI 138 TGGCCA TGG^CCA 284 TGGCCA MscI 138 TGGCCA TGG^CCA 284 TGGCCA NdeII 240 GATC ^GATC 315 GATC NlaIV 45 GGGCCC GG[ACGT]^[ACGT]CC 391 GGAACC Sau3AI 240 GATC ^GATC 315 GATC Tsp45I 25 GTGAC ^GT[GC]AC 217 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI BbvI BmyI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HphI ItaI MnlI MvaI MwoI Sau96I ScrFI TseI