(Circular) (Six-base) MAPSORT of: pFN21AB8239.seq Check: 2908 from: 1 to: 5128 pFN21AB8239 5128 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2938 278 Size: 53 83 186 936 1402 2468 Fragments arranged by size: 2468 1402 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2471 1937 Size: 534 4594 Acc65I G'GTAC_C Cuts at: 2449 2449 Size: 5128 AclI AA'CG_TT Cuts at: 1563 3255 3628 1563 Size: 1692 373 3063 Fragments arranged by size: 3063 1692 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3191 4575 4843 1632 Size: 1559 1384 268 1917 Fragments arranged by size: 1917 1559 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4931 AflIII A'CryG_T Cuts at: 1203 4027 5004 1203 Size: 2824 977 1327 Fragments arranged by size: 2824 1327 977 AgeI A'CCGG_T Cuts at: 3959 3959 Size: 5128 AhdI GACnn_n'nnGTC Cuts at: 3857 3857 Size: 5128 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5128 AlwNI CAG_nnn'CTG Cuts at: 2871 4443 2871 Size: 1572 3556 ApaI G_GGCC'C Cuts at: 2054 2054 Size: 5128 ApaLI G'TGCA_C Cuts at: 3185 4341 3185 Size: 1156 3972 ApoI r'AATT_y Cuts at: 1493 2437 2663 2815 4924 1493 Size: 944 226 152 2109 1697 Fragments arranged by size: 2109 1697 944 226 152 AseI AT'TA_AT Cuts at: 160 1032 3682 160 Size: 872 2650 1606 Fragments arranged by size: 2650 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5128 AvaI C'yCGr_G Cuts at: 1870 1943 2453 1870 Size: 73 510 4545 Fragments arranged by size: 4545 510 73 BamHI G'GATC_C Cuts at: 2458 2825 2458 Size: 367 4761 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2449 3904 618 Size: 325 217 627 662 1455 1842 Fragments arranged by size: 1842 1455 662 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2425 2447 1391 Size: 663 371 22 4072 Fragments arranged by size: 4072 663 371 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3319 3353 1903 Size: 34 1382 34 3678 Fragments arranged by size: 3678 1382 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3020 4230 3020 Size: 1210 3918 BclI T'GATC_A Cuts at: 1548 1548 Size: 5128 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 3739 136 Size: 107 122 71 631 605 394 1673 1525 Fragments arranged by size: 1673 1525 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 5123 5123 Size: 5128 BlpI GC'TnA_GC Cuts at: 2545 2545 Size: 5128 Bme1580I G_kGCm'C Cuts at: 1790 2054 3189 4345 1790 Size: 264 1135 1156 2573 Fragments arranged by size: 2573 1156 1135 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5128 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2029 2859 3817 Size: 416 53 83 399 24 612 830 958 Cuts at: 3817 442 Size: 1753 Fragments arranged by size: 1753 958 830 612 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5128 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 3788 1239 Size: 1010 1539 2579 Fragments arranged by size: 2579 1539 1010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3253 4118 4416 3253 Size: 865 298 3965 Fragments arranged by size: 3965 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3791 882 Size: 617 2292 2219 Fragments arranged by size: 2292 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2919 493 Size: 711 1715 2702 Fragments arranged by size: 2702 1715 711 BsaBI GATnn'nnATC Cuts at: 5122 5122 Size: 5128 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2935 3317 3952 275 Size: 53 83 186 936 1402 382 635 1451 Fragments arranged by size: 1451 1402 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3561 3959 4233 4380 4791 3561 Size: 398 274 147 411 3898 Fragments arranged by size: 3898 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2100 2130 2395 2425 1178 Size: 30 165 30 697 30 265 30 3881 Fragments arranged by size: 3881 697 265 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2121 2216 1186 Size: 935 95 4098 Fragments arranged by size: 4098 935 95 BseYI C'CCAG_C Cuts at: 4331 4827 4331 Size: 496 4632 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2192 1163 Size: 573 456 4099 Fragments arranged by size: 4099 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2578 3339 4367 1540 Size: 275 186 60 517 761 1028 2301 Fragments arranged by size: 2301 1028 761 517 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2410 3189 3274 4345 2410 Size: 779 85 1071 3193 Fragments arranged by size: 3193 1071 779 85 BsmI GAATG_Cn' Cuts at: 2639 2732 2639 Size: 93 5035 BsmBI CGTCTCn'nnnn_ Cuts at: 2095 2095 Size: 5128 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2410 2425 2447 3189 3274 4345 Size: 399 264 356 15 22 742 85 1071 Cuts at: 4345 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 356 264 85 22 15 BspEI T'CCGG_A Cuts at: 4791 4791 Size: 5128 BspHI T'CATG_A Cuts at: 3017 4747 3017 Size: 1730 3398 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2483 844 Size: 1639 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2337 2380 3015 1394 Size: 429 514 43 635 3507 Fragments arranged by size: 3507 635 514 429 43 BsrDI GCAATG_nn' Cuts at: 65 1248 3623 3797 65 Size: 1183 2375 174 1396 Fragments arranged by size: 2375 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3772 3959 1700 Size: 110 1962 187 2869 Fragments arranged by size: 2869 1962 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5128 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5128 BssSI C'ACGA_G Cuts at: 2409 3188 4200 2409 Size: 779 1012 3337 Fragments arranged by size: 3337 1012 779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5128 BstBI TT'CG_AA Cuts at: 3932 3932 Size: 5128 BstEII G'GTnAC_C Cuts at: 1184 2221 1184 Size: 1037 4091 BstXI CCAn_nnnn'nTGG Cuts at: 2366 2392 2366 Size: 26 5102 BstYI r'GATC_y Cuts at: 1972 2458 2825 3210 3227 4679 4765 4777 5123 Size: 486 367 385 17 1452 86 12 346 Cuts at: 5123 1972 Size: 1977 Fragments arranged by size: 1977 1452 486 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3637 Fragments arranged by size: 3637 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4389 Fragments arranged by size: 4389 683 56 BtsI GCAGTG_nn' Cuts at: 2644 3438 3458 2644 Size: 794 20 4314 Fragments arranged by size: 4314 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5128 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5093 DraI TTT'AAA Cuts at: 2432 2432 Size: 5128 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4135 809 Size: 507 2819 1802 Fragments arranged by size: 2819 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2058 2143 Size: 54 1425 50 275 18 177 51 85 Cuts at: 2143 2178 2289 2575 3464 5093 8 Size: 35 111 286 889 1629 43 Fragments arranged by size: 1629 1425 889 286 275 177 111 85 54 51 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 2575 1537 Size: 275 246 517 4090 Fragments arranged by size: 4090 517 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 3058 1353 Size: 1705 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3715 4089 4235 387 Size: 1064 138 2126 374 146 1280 Fragments arranged by size: 2126 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 3191 3788 4575 4843 1239 Size: 393 617 942 597 787 268 1524 Fragments arranged by size: 1524 942 787 617 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2050 2051 2284 2050 Size: 1 233 4894 Fragments arranged by size: 4894 233 1 EcoRI G'AATT_C Cuts at: 2437 2815 2437 Size: 378 4750 FspI TGC'GCA Cuts at: 2042 3634 2042 Size: 1592 3536 HaeII r_GCGC'y Cuts at: 1678 4275 1678 Size: 2597 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3769 3801 3843 3875 1373 Size: 32 135 32 2197 32 42 32 2626 Fragments arranged by size: 2626 2197 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2472 2718 669 Size: 988 281 534 246 3079 Fragments arranged by size: 3079 988 534 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5128 HpaI GTT'AAC Cuts at: 2718 2718 Size: 5128 KpnI G_GTAC'C Cuts at: 2453 2453 Size: 5128 MfeI C'AATT_G Cuts at: 2727 2727 Size: 5128 MluI A'CGCG_T Cuts at: 5004 5004 Size: 5128 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4242 4426 1305 Size: 2937 184 2007 Fragments arranged by size: 2937 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 2291 10 Size: 54 1425 343 313 146 2847 Fragments arranged by size: 2847 1425 343 313 146 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 3086 3445 3604 518 Size: 648 1224 696 359 159 2042 Fragments arranged by size: 2042 1224 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2544 3221 4369 4614 1606 Size: 938 677 1148 245 2120 Fragments arranged by size: 2120 1148 938 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3637 Fragments arranged by size: 3637 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5128 NotI GC'GGCC_GC Cuts at: 2575 2575 Size: 5128 NspI r_CATG'y Cuts at: 1132 1229 2486 4031 1132 Size: 97 1257 1545 2229 Fragments arranged by size: 2229 1545 1257 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5128 PciI A'CATG_T Cuts at: 4027 4027 Size: 5128 PflMI CCAn_nnn'nTGG Cuts at: 1108 2133 1108 Size: 1025 4103 PmeI GTTT'AAAC Cuts at: 2432 2432 Size: 5128 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3197 3229 4710 4742 3197 Size: 32 1481 32 3583 Fragments arranged by size: 3583 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2284 Size: 5128 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5128 PsiI TTA'TAA Cuts at: 2698 2698 Size: 5128 PspOMI G'GGCC_C Cuts at: 2050 2050 Size: 5128 PstI C_TGCA'G Cuts at: 830 2480 830 Size: 1650 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5128 SalI G'TCGA_C Cuts at: 1936 2470 1936 Size: 534 4594 SbfI CC_TGCA'GG Cuts at: 2480 2480 Size: 5128 ScaI AGT'ACT Cuts at: 3376 5088 3376 Size: 1712 3416 SexAI A'CCwGG_T Cuts at: 2358 2358 Size: 5128 SfcI C'TryA_G Cuts at: 826 1045 2476 3611 4292 4483 826 Size: 219 1431 1135 681 191 1471 Fragments arranged by size: 1471 1431 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2066 2066 Size: 5128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5128 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5128 SmaI CCC'GGG Cuts at: 1872 2455 1872 Size: 583 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3232 4133 4395 820 Size: 197 926 1289 901 262 1553 Fragments arranged by size: 1553 1289 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2919 493 Size: 2426 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5128 SphI G_CATG'C Cuts at: 1132 2486 1132 Size: 1354 3774 SspI AAT'ATT Cuts at: 5 52 3052 5 Size: 47 3000 2081 Fragments arranged by size: 3000 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5128 StyI C'CwwG_G Cuts at: 513 1065 2004 2567 513 Size: 552 939 563 3074 Fragments arranged by size: 3074 939 563 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3155 3172 3325 3510 3955 325 Size: 1255 1575 17 153 185 445 1498 Fragments arranged by size: 1575 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3374 5086 96 Size: 274 80 33 51 1443 1397 1712 138 Fragments arranged by size: 1712 1443 1397 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 2395 2427 5088 5120 2100 Size: 32 263 32 2661 32 2108 Fragments arranged by size: 2661 2108 263 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5128 XbaI T'CTAG_A Cuts at: 2464 2464 Size: 5128 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5128 XmaI C'CCGG_G Cuts at: 1870 2453 1870 Size: 583 4545 XmnI GAAnn'nnTTC Cuts at: 3257 3257 Size: 5128 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2936 276 Size: 53 83 186 936 1402 2468 Fragments arranged by size: 2468 1402 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfoI SrfI SwaI XcmI