Restriction map for
pFN21AB3853
(Linear) (Six-base) MAPSORT of: KIBB3853.seq Check: 8053 from: 1 to: 636
KIBB3853.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 203 636
Size: 203 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 614 636
Size: 447 167 22
Fragments arranged by size:
447 167 22
AgeI A'CCGG_T
Cuts at: 0 198 636
Size: 198 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 381 636
Size: 381 255
AlwNI CAG_nnn'CTG
Cuts at: 0 301 369 636
Size: 301 68 267
Fragments arranged by size:
301 267 68
ApaI G_GGCC'C
Cuts at: 0 377 636
Size: 377 259
ApaLI G'TGCA_C
Cuts at: 0 315 636
Size: 315 321
ApoI r'AATT_y
Cuts at: 0 121 436 636
Size: 121 315 200
Fragments arranged by size:
315 200 121
BamHI G'GATC_C
Cuts at: 0 160 636
Size: 160 476
BanII G_rGCy'C
Cuts at: 0 377 636
Size: 377 259
BbsI GAAGACnn'nnnn_
Cuts at: 0 339 411 620 636
Size: 339 72 209 16
Fragments arranged by size:
339 209 72 16
BbvCI CC'TCA_GC
Cuts at: 0 151 636
Size: 151 485
Bme1580I G_kGCm'C
Cuts at: 0 319 377 636
Size: 319 58 259
Fragments arranged by size:
319 259 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 183 357 636
Size: 183 174 279
Fragments arranged by size:
279 183 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 636
Size: 409 227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 635 636
Size: 575 60 1
Fragments arranged by size:
575 60 1
Bpu10I CC'TnA_GC
Cuts at: 0 151 636
Size: 151 485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 636
Size: 77 559
BsaBI GATnn'nnATC
Cuts at: 0 331 636
Size: 331 305
BsaWI w'CCGG_w
Cuts at: 0 198 636
Size: 198 438
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 604 636
Size: 139 465 32
Fragments arranged by size:
465 139 32
BseYI C'CCAG_C
Cuts at: 0 476 636
Size: 476 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 636
Size: 274 362
BsiEI CG_ry'CG
Cuts at: 0 203 636
Size: 203 433
BsiHKAI G_wGCw'C
Cuts at: 0 319 636
Size: 319 317
BsmBI CGTCTCn'nnnn_
Cuts at: 0 523 636
Size: 523 113
Bsp1286I G_dGCh'C
Cuts at: 0 319 377 636
Size: 319 58 259
Fragments arranged by size:
319 259 58
BsrDI GCAATG_nn'
Cuts at: 0 239 636
Size: 239 397
BsrFI r'CCGG_y
Cuts at: 0 198 636
Size: 198 438
BstYI r'GATC_y
Cuts at: 0 160 636
Size: 160 476
BtgI C'CryG_G
Cuts at: 0 248 308 636
Size: 248 60 328
Fragments arranged by size:
328 248 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 318 339 636
Size: 318 21 297
Fragments arranged by size:
318 297 21
BtsI GCAGTG_nn'
Cuts at: 0 262 302 636
Size: 262 40 334
Fragments arranged by size:
334 262 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 159 194 636
Size: 159 35 442
Fragments arranged by size:
442 159 35
DraI TTT'AAA
Cuts at: 0 453 636
Size: 453 183
EaeI y'GGCC_r
Cuts at: 0 128 264 279 305 636
Size: 128 136 15 26 331
Fragments arranged by size:
331 136 128 26 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 575 614 635 636
Size: 447 128 39 21 1
Fragments arranged by size:
447 128 39 21 1
EcoO109I rG'GnC_Cy
Cuts at: 0 373 636
Size: 373 263
EcoRI G'AATT_C
Cuts at: 0 436 636
Size: 436 200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 636
Size: 363 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 47 79 152 184 610 636
Size: 47 32 73 32 426 26
Fragments arranged by size:
426 73 47 32 32 26
HincII GTy'rAC
Cuts at: 0 204 579 636
Size: 204 375 57
Fragments arranged by size:
375 204 57
HindIII A'AGCT_T
Cuts at: 0 95 636
Size: 95 541
MscI TGG'CCA
Cuts at: 0 130 266 281 307 636
Size: 130 136 15 26 329
Fragments arranged by size:
329 136 130 26 15
MslI CAynn'nnrTG
Cuts at: 0 67 502 636
Size: 67 435 134
Fragments arranged by size:
435 134 67
MspA1I CmG'CkG
Cuts at: 0 118 275 636
Size: 118 157 361
Fragments arranged by size:
361 157 118
NcoI C'CATG_G
Cuts at: 0 248 308 636
Size: 248 60 328
Fragments arranged by size:
328 248 60
NspI r_CATG'y
Cuts at: 0 568 636
Size: 568 68
PflMI CCAn_nnn'nTGG
Cuts at: 0 173 369 636
Size: 173 196 267
Fragments arranged by size:
267 196 173
PspOMI G'GGCC_C
Cuts at: 0 373 636
Size: 373 263
PstI C_TGCA'G
Cuts at: 0 297 636
Size: 297 339
SalI G'TCGA_C
Cuts at: 0 202 636
Size: 202 434
SfcI C'TryA_G
Cuts at: 0 293 636
Size: 293 343
SmlI C'TyrA_G
Cuts at: 0 92 636
Size: 92 544
StyI C'CwwG_G
Cuts at: 0 248 308 636
Size: 248 60 328
Fragments arranged by size:
328 248 60
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 274 636
Size: 274 362
Enzymes that do cut:
AccI AcuI AgeI AlfI AlwNI ApaI ApaLI ApoI
BamHI BanII BbsI BbvCI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaBI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BstYI BtgI BtgZI
BtsI CspCI DraI EaeI Eco57MI EcoO109I EcoRI FalI
Hin4I HincII HindIII MscI MslI MspA1I NcoI NspI
PflMI PspOMI PstI SalI SfcI SmlI StyI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BsaI BsaAI BsaHI BsaXI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI