(Circular) (Six-base) MAPSORT of: pFN21AA0228.seq Check: 8496 from: 1 to: 6460 pFN21AA0228 6460 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4270 278 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3803 1937 Size: 1866 4594 Acc65I G'GTAC_C Cuts at: 3781 3781 Size: 6460 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 2429 3132 3369 3726 4523 5907 6175 Size: 639 158 703 237 357 797 1384 268 Cuts at: 6175 1632 Size: 1917 Fragments arranged by size: 1917 1384 797 703 639 357 268 237 158 AflII C'TTAA_G Cuts at: 820 1017 2749 820 Size: 197 1732 4531 Fragments arranged by size: 4531 1732 197 AflIII A'CryG_T Cuts at: 1203 5359 6336 1203 Size: 4156 977 1327 Fragments arranged by size: 4156 1327 977 AgeI A'CCGG_T Cuts at: 3636 5291 3636 Size: 1655 4805 AhdI GACnn_n'nnGTC Cuts at: 5189 5189 Size: 6460 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6460 AlwNI CAG_nnn'CTG Cuts at: 2342 3208 4203 5775 2342 Size: 866 995 1572 3027 Fragments arranged by size: 3027 1572 995 866 ApaLI G'TGCA_C Cuts at: 2643 4517 5673 2643 Size: 1874 1156 3430 Fragments arranged by size: 3430 1874 1156 ApoI r'AATT_y Cuts at: 1493 2180 2786 3050 3272 3335 3769 3995 4147 Size: 687 606 264 222 63 434 226 152 Cuts at: 4147 6256 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 687 606 434 264 226 222 152 63 AseI AT'TA_AT Cuts at: 160 1032 5014 160 Size: 872 3982 1606 Fragments arranged by size: 3982 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 2111 3785 1870 Size: 73 168 1674 4545 Fragments arranged by size: 4545 1674 168 73 BamHI G'GATC_C Cuts at: 2028 2846 3790 4157 2028 Size: 818 944 367 4331 Fragments arranged by size: 4331 944 818 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3781 5236 618 Size: 325 217 627 1994 1455 1842 Fragments arranged by size: 1994 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3779 1391 Size: 2388 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3231 3319 928 Size: 971 1332 88 4069 Fragments arranged by size: 4069 1332 971 88 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4651 4685 1903 Size: 34 2714 34 3678 Fragments arranged by size: 3678 2714 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4352 5562 4352 Size: 1210 5250 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5071 136 Size: 107 122 71 631 605 337 3062 1525 Fragments arranged by size: 3062 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6455 6455 Size: 6460 BlpI GC'TnA_GC Cuts at: 3877 3877 Size: 6460 Bme1580I G_kGCm'C Cuts at: 1790 2647 4521 5677 1790 Size: 857 1874 1156 2573 Fragments arranged by size: 2573 1874 1156 857 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6460 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2180 3691 4191 Size: 416 53 83 399 24 763 1511 500 Cuts at: 4191 5149 442 Size: 958 1753 Fragments arranged by size: 1753 1511 958 763 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5120 1239 Size: 3881 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4585 5450 5748 4585 Size: 865 298 5297 Fragments arranged by size: 5297 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3566 5123 882 Size: 617 2067 1557 2219 Fragments arranged by size: 2219 2067 1557 617 BsaAI yAC'GTr Cuts at: 493 1204 2989 4251 493 Size: 711 1785 1262 2702 Fragments arranged by size: 2702 1785 1262 711 BsaBI GATnn'nnATC Cuts at: 6454 6454 Size: 6460 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4267 4649 5284 275 Size: 53 83 186 936 2734 382 635 1451 Fragments arranged by size: 2734 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3636 4893 5291 5565 5712 6123 3636 Size: 1257 398 274 147 411 3973 Fragments arranged by size: 3973 1257 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2087 2099 2117 1178 Size: 30 165 30 666 18 12 18 5521 Fragments arranged by size: 5521 666 165 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3179 1186 Size: 1993 4467 BseYI C'CCAG_C Cuts at: 5663 6159 5663 Size: 496 5964 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 2607 3737 1163 Size: 573 772 99 1130 3886 Fragments arranged by size: 3886 1130 772 573 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 3910 4671 5699 1540 Size: 275 186 12 1897 761 1028 2301 Fragments arranged by size: 2301 1897 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2321 2647 4521 4606 5677 2321 Size: 326 1874 85 1071 3104 Fragments arranged by size: 3104 1874 1071 326 85 BsmI GAATG_Cn' Cuts at: 3971 4064 3971 Size: 93 6367 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2321 2647 3779 4521 4606 5677 1391 Size: 399 531 326 1132 742 85 1071 2174 Fragments arranged by size: 2174 1132 1071 742 531 399 326 85 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 3001 3223 4349 6079 3001 Size: 222 1126 1730 3382 Fragments arranged by size: 3382 1730 1126 222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3815 844 Size: 2971 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2085 2103 4347 1394 Size: 429 262 18 2244 3507 Fragments arranged by size: 3507 2244 429 262 18 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 4955 5129 65 Size: 1183 1458 2249 174 1396 Fragments arranged by size: 2249 1458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3636 5104 5291 1700 Size: 110 1826 1468 187 2869 Fragments arranged by size: 2869 1826 1468 187 110 BsrGI T'GTAC_A Cuts at: 96 3280 96 Size: 3184 3276 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6460 BssSI C'ACGA_G Cuts at: 4520 5532 4520 Size: 1012 5448 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6460 BstBI TT'CG_AA Cuts at: 5264 5264 Size: 6460 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6460 BstYI r'GATC_y Cuts at: 1972 2028 2846 3790 4157 4542 4559 6011 6097 Size: 56 818 944 367 385 17 1452 86 Cuts at: 6097 6109 6455 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 944 818 385 367 346 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 513 Size: 552 939 9 4960 Fragments arranged by size: 4960 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5721 Fragments arranged by size: 5721 683 56 BtsI GCAGTG_nn' Cuts at: 2182 2826 2873 3976 4770 4790 2182 Size: 644 47 1103 794 20 3852 Fragments arranged by size: 3852 1103 794 644 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6425 DraI TTT'AAA Cuts at: 2563 3764 2563 Size: 1201 5259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2163 5467 809 Size: 507 847 3304 1802 Fragments arranged by size: 3304 1802 847 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2602 3907 Size: 54 1425 50 275 18 180 592 1305 Cuts at: 3907 4796 6425 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1305 889 592 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 3907 1537 Size: 275 198 1897 4090 Fragments arranged by size: 4090 1897 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 3025 4390 1353 Size: 1672 1365 3423 Fragments arranged by size: 3423 1672 1365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5047 5421 5567 387 Size: 1064 138 3458 374 146 1280 Fragments arranged by size: 3458 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2429 3132 3369 3726 4523 5120 Size: 393 639 158 703 237 357 797 597 Cuts at: 5120 5907 6175 1239 Size: 787 268 1524 Fragments arranged by size: 1524 797 787 703 639 597 393 357 268 237 158 EcoRI G'AATT_C Cuts at: 3769 4147 3769 Size: 378 6082 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2775 3137 3404 3434 2775 Size: 362 267 30 5801 Fragments arranged by size: 5801 362 267 30 FspI TGC'GCA Cuts at: 4966 4966 Size: 6460 HaeII r_GCGC'y Cuts at: 1678 5607 1678 Size: 3929 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 2576 2608 3655 Size: 32 135 32 515 32 457 32 1047 Cuts at: 3655 3687 5101 5133 5175 5207 1373 Size: 32 1414 32 42 32 2626 Fragments arranged by size: 2626 1414 1047 515 457 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2168 3804 4050 669 Size: 988 281 230 1636 246 3079 Fragments arranged by size: 3079 1636 988 281 246 230 HindIII A'AGCT_T Cuts at: 748 2141 2757 3261 748 Size: 1393 616 504 3947 Fragments arranged by size: 3947 1393 616 504 HpaI GTT'AAC Cuts at: 4050 4050 Size: 6460 KpnI G_GTAC'C Cuts at: 3785 3785 Size: 6460 MfeI C'AATT_G Cuts at: 3174 3504 4059 3174 Size: 330 555 5575 Fragments arranged by size: 5575 555 330 MluI A'CGCG_T Cuts at: 6336 6336 Size: 6460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3271 5574 5758 1305 Size: 1966 2303 184 2007 Fragments arranged by size: 2303 2007 1966 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2604 10 Size: 54 1425 343 772 3866 Fragments arranged by size: 3866 1425 772 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 2670 2683 4418 4777 4936 518 Size: 648 1381 123 13 1735 359 159 2042 Fragments arranged by size: 2042 1735 1381 648 359 159 123 13 MspA1I CmG'CkG Cuts at: 1606 2063 3876 4553 5701 5946 1606 Size: 457 1813 677 1148 245 2120 Fragments arranged by size: 2120 1813 1148 677 457 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4969 Fragments arranged by size: 4969 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6460 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6460 NotI GC'GGCC_GC Cuts at: 3907 3907 Size: 6460 NspI r_CATG'y Cuts at: 1132 1229 2819 3818 5363 1132 Size: 97 1590 999 1545 2229 Fragments arranged by size: 2229 1590 1545 999 97 PacI TTA_AT'TAA Cuts at: 2811 2811 Size: 6460 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6460 PciI A'CATG_T Cuts at: 5359 5359 Size: 6460 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6460 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4529 4561 6042 6074 4529 Size: 32 1481 32 4915 Fragments arranged by size: 4915 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6460 PsiI TTA'TAA Cuts at: 4030 4030 Size: 6460 PstI C_TGCA'G Cuts at: 830 2347 3812 830 Size: 1517 1465 3478 Fragments arranged by size: 3478 1517 1465 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 2211 4708 6420 2211 Size: 2497 1712 2251 Fragments arranged by size: 2497 2251 1712 SfcI C'TryA_G Cuts at: 826 1045 2343 3498 3532 3808 4943 5624 5815 Size: 219 1298 1155 34 276 1135 681 191 Cuts at: 5815 826 Size: 1471 Fragments arranged by size: 1471 1298 1155 1135 681 276 219 191 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3787 1872 Size: 1915 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2749 4564 5465 5727 820 Size: 197 926 806 1815 901 262 1553 Fragments arranged by size: 1815 1553 926 901 806 262 197 SnaBI TAC'GTA Cuts at: 493 4251 493 Size: 3758 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3818 1132 Size: 2686 3774 SspI AAT'ATT Cuts at: 5 52 2864 3656 4384 5 Size: 47 2812 792 728 2081 Fragments arranged by size: 2812 2081 792 728 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6460 StyI C'CwwG_G Cuts at: 513 1065 2004 3307 3899 513 Size: 552 939 1303 592 3074 Fragments arranged by size: 3074 1303 939 592 552 SwaI ATTT'AAAT Cuts at: 2563 2563 Size: 6460 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2537 2871 3766 4487 4504 4657 4842 Size: 1255 957 334 895 721 17 153 185 Cuts at: 4842 5287 325 Size: 445 1498 Fragments arranged by size: 1498 1255 957 895 721 445 334 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2209 2453 3280 Size: 274 80 33 51 1443 232 244 827 Cuts at: 3280 4706 6418 96 Size: 1426 1712 138 Fragments arranged by size: 1712 1443 1426 827 274 244 232 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2009 2041 6420 6452 2009 Size: 32 4379 32 2017 Fragments arranged by size: 4379 2017 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6460 XbaI T'CTAG_A Cuts at: 3796 3796 Size: 6460 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3041 3709 2011 Size: 1030 668 4762 Fragments arranged by size: 4762 1030 668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3785 1870 Size: 1915 4545 XmnI GAAnn'nnTTC Cuts at: 3387 4589 3387 Size: 1202 5258 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4268 276 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI