################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HapII HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TspRI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 157 GGCGTC G[GA]^CG[CT]C BalI 323 TGGCCA TGG^CCA BbvI 407 GCAGC GCAGC(8/12) BpmI 565 CTCCAG CTGGAG(16/14) BsaHI 157 GGCGTC G[GA]^CG[CT]C BsaI 184 GAGACC GGTCTC(1/5) BsgI 299 GTGCAG GTGCAG(16/14) BsiEI 84 CGATCG CG[GA][CT]^CG Bsp1407I 482 TGTACA T^GTACA BsrBI 132 CCGCTC CCGCTC(-3/-3) BsrDI 31 GCAATG GCAATG(2/0) BsrGI 482 TGTACA T^GTACA BsrI 378 CCAGT ACTGG(1/-1) BstEII 261 GGTCACC G^GT[ACGT]ACC BstPI 261 GGTCACC G^GT[ACGT]ACC Cac8I 361 GCCCGC GC[ACGT]^[ACGT]GC CfoI 129 GCGC GCG^C DsaI 113 CCGTGG C^C[GA][CT]GG EcoNI 411 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 261 GGTCACC G^GT[ACGT]ACC EcoT14I 508 CCTTGG C^C[AT][AT]GG FauI 362 CCCGC CCCGC(4/6) HaeII 128 AGCGCC [GA]GCGC^[CT] HgaI 158 GCGTC GACGC(5/10) HhaI 129 GCGC GCG^C Hin1I 157 GGCGTC G[GA]^CG[CT]C HinP1I 129 GCGC G^CGC MaeII 338 ACGT A^CGT MluNI 323 TGGCCA TGG^CCA MscI 323 TGGCCA TGG^CCA MslI 190 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 498 GCCATTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PshAI 153 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 84 CGATCG CGAT^CG SspBI 482 TGTACA T^GTACA SspI 461 AATATT AAT^ATT StyI 508 CCTTGG C^C[AT][AT]GG TaqI 534 TCGA T^CGA TseI 407 GCAGC G^C[AT]GC Tsp45I 262 GTCAC ^GT[GC]AC Tsp509I 59 AATT ^AATT TthHB8I 534 TCGA T^CGA XcmI 499 CCATTCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AhdI 186 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 232 GACATACTGTC AspEI 186 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 232 GACATACTGTC BanII 282 GAGCCC G[GA]GC[CT]^C 400 GGGCTC BbsI 150 GAAGAC GAAGAC(2/6) 160 GTCTTC BciVI 36 GTATCC GTATCC(6/5) 381 GTATCC BmyI 282 GAGCCC G[AGT]GC[ACT]^C 400 GGGCTC BpuAI 150 GAAGAC GAAGAC(2/6) 160 GTCTTC BsaJI 113 CCGTGG C^C[ACGT][ACGT]GG 508 CCTTGG Bsp1286I 282 GAGCCC G[AGT]GC[ACT]^C 400 GGGCTC DraI 61 TTTAAA TTT^AAA 457 TTTAAA EaeI 311 TGGCCG [CT]^GGCC[GA] 323 TGGCCA Eam1105I 186 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 232 GACATACTGTC Fnu4HI 131 GCCGC GC^[ACGT]GC 407 GCAGC HaeIII 312 GGCC GG^CC 324 GGCC ItaI 131 GCCGC GC^[ACGT]GC 407 GCAGC MaeIII 262 GTCAC ^GT[ACGT]AC 389 GTTAC NlaIV 281 GGAGCC GG[ACGT]^[ACGT]CC 401 GGCTCC PleI 414 GAGTC GAGTC(4/5) 561 GAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AciI AfaI BsiYI BslI BsmAI BssKI BstNI DdeI DpnII EcoRII HinfI HphI MboI MboII MnlI MseI MvaI NdeII NlaIII RsaI Sau3AI ScrFI SexAI TfiI Tru9I