Restriction map for
pFN21AE0553
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BalI BbsI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FbaI FseI HgaI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TspRI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 430 CGCG CG^CG
AcyI 632 GGCGCC G[GA]^CG[CT]C
AflIII 828 ACACGT A^C[GA][CT]GT
ApaI 914 GGGCCC GGGCC^C
AviII 839 TGCGCA TGC^GCA
BamHI 579 GGATCC G^GATCC
BbeI 632 GGCGCC GGCGC^C
BbrPI 829 CACGTG CAC^GTG
BciVI 274 GTATCC GTATCC(6/5)
BfaI 1009 CTAG C^TAG
BglII 737 AGATCT A^GATCT
BsaAI 829 CACGTG [CT]AC^GT[GA]
BsaHI 632 GGCGCC G[GA]^CG[CT]C
BseRI 1012 GAGGAG GAGGAG(10/8)
BsgI 541 CTGCAC GTGCAG(16/14)
BsmI 535 GCATTC GAATGC(1/-1)
Bsp1407I 482 TGTACA T^GTACA
BspHI 929 TCATGA T^CATGA
BsrGI 482 TGTACA T^GTACA
BsrI 377 ACTGG ACTGG(1/-1)
BstUI 430 CGCG CG^CG
EaeI 311 TGGCCG [CT]^GGCC[GA]
EcoNI 876 CCTATATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 316 GAATTC G^AATTC
EcoT14I 508 CCTTGG C^C[AT][AT]GG
EcoT22I 533 ATGCAT ATGCA^T
FspI 839 TGCGCA TGC^GCA
Hin1I 632 GGCGCC G[GA]^CG[CT]C
KasI 632 GGCGCC G^GCGCC
MaeI 1009 CTAG C^TAG
MaeII 830 ACGT A^CGT
MseI 605 TTAA T^TAA
MspA1I 235 CAGCTG C[AC]G^C[GT]G
MvnI 430 CGCG CG^CG
NarI 632 GGCGCC GG^CGCC
NsiI 533 ATGCAT ATGCA^T
PflMI 696 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 414 GAGTC GAGTC(4/5)
PmaCI 829 CACGTG CAC^GTG
PmlI 829 CACGTG CAC^GTG
PpuMI 683 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 405 CTGCAG CTGCA^G
PvuII 235 CAGCTG CAG^CTG
RcaI 929 TCATGA T^CATGA
ScaI 950 AGTACT AGT^ACT
SexAI 265 ACCTGGT A^CC[AT]GGT
SfoI 632 GGCGCC GGC^GCC
SspBI 482 TGTACA T^GTACA
StyI 508 CCTTGG C^C[AT][AT]GG
Tru9I 605 TTAA T^TAA
Van91I 696 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 580 GATCC GGATC(4/5)
579 GGATC
AspHI 328 GAGCAC G[AT]GC[AT]^C
1015 GAGCAC
BanI 632 GGCGCC G^G[CT][GA]CC
676 GGCACC
BpmI 1054 CTCCAG CTGGAG(16/14)
765 CTGGAG
BsiHKAI 328 GAGCAC G[AT]GC[AT]^C
1015 GAGCAC
BsrBI 132 CCGCTC CCGCTC(-3/-3)
28 GAGCGG
BstAPI 498 GCAATTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
894 GCAAATGCTGC
BstYI 579 GGATCC [GA]^GATC[CT]
737 AGATCT
DraII 683 GGGTCCT [GA]G^G[ACGT]CC[CT]
913 GGGGCCC
DrdI 151 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
613 GACCCTTTGGTC
EcoO109I 683 GGGTCCT [GA]G^G[ACGT]CC[CT]
913 GGGGCCC
HphI 263 TCACC GGTGA(8/7)
341 TCACC
MaeIII 340 GTCAC ^GT[ACGT]AC
748 GTCAC
MflI 579 GGATCC [GA]^GATC[CT]
737 AGATCT
SfcI 405 CTGCAG C^T[GA][CT]AG
741 CTACAG
Tsp45I 340 GTCAC ^GT[GC]AC
748 GTCAC
XcmI 993 CCAGCCCTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1023 CCAAGCCCTGAGTGG
XhoII 579 GGATCC [GA]^GATC[CT]
737 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI TseI
Tsp509I TthHB8I