(Circular) (Six-base) MAPSORT of: pFN21AE0553.seq Check: 9761 from: 1 to: 5776 pFN21AE0553 5776 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3075 3903 4276 1563 Size: 1512 828 373 3063 Fragments arranged by size: 3063 1512 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 3839 5223 5491 1632 Size: 1172 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1172 1035 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2833 4675 5652 1203 Size: 1630 1842 977 1327 Fragments arranged by size: 1842 1630 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 2834 2972 1166 Size: 1668 138 3970 Fragments arranged by size: 3970 1668 138 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2227 2258 1184 Size: 1043 31 4702 Fragments arranged by size: 4702 1043 31 AlwNI CAG_nnn'CTG Cuts at: 3519 5091 3519 Size: 1572 4204 ApaI G_GGCC'C Cuts at: 2923 2923 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2321 2459 2522 3085 3311 3463 5572 1493 Size: 828 138 63 563 226 152 2109 1697 Fragments arranged by size: 2109 1697 828 563 226 152 138 63 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3101 1870 Size: 73 1158 4545 Fragments arranged by size: 4545 1158 73 BamHI G'GATC_C Cuts at: 2584 3106 3473 2584 Size: 522 367 4887 Fragments arranged by size: 4887 522 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2637 2681 3097 4552 618 Size: 325 217 627 850 44 416 1455 1842 Fragments arranged by size: 1842 1455 850 627 416 325 217 44 BanII G_rGCy'C Cuts at: 1391 2434 2777 2923 3095 1391 Size: 1043 343 146 172 4072 Fragments arranged by size: 4072 1043 343 172 146 BbeI G_GCGC'C Cuts at: 2641 2641 Size: 5776 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2290 3668 4878 2290 Size: 1378 1210 3188 Fragments arranged by size: 3188 1378 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BfrBI ATG'CAT Cuts at: 2540 2540 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4387 136 Size: 107 122 71 631 605 2715 1525 Fragments arranged by size: 2715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2742 5771 2742 Size: 3029 2747 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2923 3837 4993 1790 Size: 1133 914 1156 2573 Fragments arranged by size: 2573 1156 1133 914 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2392 3507 4465 Size: 416 53 83 399 24 975 1115 958 Cuts at: 4465 442 Size: 1753 Fragments arranged by size: 1753 1115 975 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2791 3044 4436 1239 Size: 1552 253 1392 2579 Fragments arranged by size: 2579 1552 1392 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2836 3567 493 Size: 711 1632 731 2702 Fragments arranged by size: 2702 1632 731 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2638 3583 3965 4600 Size: 53 83 186 936 1105 945 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1451 1105 945 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 2421 2451 1178 Size: 30 165 30 621 30 367 30 4503 Fragments arranged by size: 4503 621 367 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3032 1186 Size: 1846 3930 BseYI C'CCAG_C Cuts at: 2472 2915 2961 4979 5475 2472 Size: 443 46 2018 496 2773 Fragments arranged by size: 2773 2018 496 443 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2531 1163 Size: 573 795 4408 Fragments arranged by size: 4408 795 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 3024 3837 3922 4993 2337 Size: 687 813 85 1071 3120 Fragments arranged by size: 3120 1071 813 687 85 BsmI GAATG_Cn' Cuts at: 2540 3287 3380 2540 Size: 747 93 4936 Fragments arranged by size: 4936 747 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2434 2777 2923 3024 3095 3837 Size: 399 547 97 343 146 101 71 742 Cuts at: 3837 3922 4993 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 547 399 343 146 101 97 85 71 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 2934 3665 5395 2934 Size: 731 1730 3315 Fragments arranged by size: 3315 1730 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3131 844 Size: 2287 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2035 2139 3663 1394 Size: 429 212 104 1524 3507 Fragments arranged by size: 3507 1524 429 212 104 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 2487 96 Size: 2391 3385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 2509 2905 794 Size: 1715 396 3665 Fragments arranged by size: 3665 1715 396 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2584 2742 3106 3473 3858 3875 5327 5413 Size: 612 158 364 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 612 385 367 364 346 158 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2162 2624 4783 809 Size: 507 846 462 2159 1802 Fragments arranged by size: 2159 1802 846 507 462 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 3223 4112 Size: 54 1425 50 275 18 486 907 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 907 889 486 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3706 1353 Size: 2353 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2350 4363 4737 4883 387 Size: 1064 138 761 2013 374 146 1280 Fragments arranged by size: 2013 1280 1064 761 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2791 2804 3044 3839 4436 5223 5491 Size: 393 1159 13 240 795 597 787 268 Cuts at: 5491 1239 Size: 1524 Fragments arranged by size: 1524 1159 795 787 597 393 268 240 13 EcoNI CCTnn'n_nnAGG Cuts at: 2885 2885 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2689 2919 2689 Size: 230 5546 EcoRI G'AATT_C Cuts at: 2321 3085 3463 2321 Size: 764 378 4634 Fragments arranged by size: 4634 764 378 FspI TGC'GCA Cuts at: 2846 4282 2846 Size: 1436 4340 HaeII r_GCGC'y Cuts at: 1678 2408 2641 2671 2897 4923 1678 Size: 730 233 30 226 2026 2531 Fragments arranged by size: 2531 2026 730 233 226 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2832 2864 4417 Size: 32 135 32 436 32 792 32 1553 Cuts at: 4417 4449 4491 4523 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1553 792 436 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2637 2637 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2072 2709 4890 5074 1305 Size: 767 637 2181 184 2007 Fragments arranged by size: 2181 2007 767 637 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2340 2834 2972 3734 4093 4252 518 Size: 648 1174 494 138 762 359 159 2042 Fragments arranged by size: 2042 1174 762 648 494 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2242 3192 3869 5017 5262 1606 Size: 636 950 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 677 636 245 NarI GG'CG_CC Cuts at: 2638 2638 Size: 5776 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NsiI A_TGCA'T Cuts at: 2542 2542 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 3003 1348 Size: 1655 4121 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2707 1108 Size: 1599 4177 PfoI T'CCnGG_A Cuts at: 2282 2768 2282 Size: 486 5290 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PmlI CAC'GTG Cuts at: 2836 2836 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2067 2099 3845 3877 5358 5390 2067 Size: 32 1746 32 1481 32 2453 Fragments arranged by size: 2453 1746 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2689 2689 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2919 2919 Size: 5776 PstI C_TGCA'G Cuts at: 830 2414 3128 830 Size: 1584 714 3478 Fragments arranged by size: 3478 1584 714 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2242 2242 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2957 4024 5736 2957 Size: 1067 1712 2997 Fragments arranged by size: 2997 1712 1067 SexAI A'CCwGG_T Cuts at: 2270 2270 Size: 5776 SfcI C'TryA_G Cuts at: 826 1045 2410 2746 3124 4259 4940 5131 826 Size: 219 1365 336 378 1135 681 191 1471 Fragments arranged by size: 1471 1365 1135 681 378 336 219 191 SfoI GGC'GCC Cuts at: 2639 2639 Size: 5776 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2513 3215 513 Size: 552 1448 702 3074 Fragments arranged by size: 3074 1448 702 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 2955 4022 Size: 274 80 33 51 1443 510 468 1067 Cuts at: 4022 5734 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1067 510 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 5736 5768 2421 Size: 32 3283 32 2429 Fragments arranged by size: 3283 2429 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3005 3035 3005 Size: 30 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI