Restriction map for
pFN21AE0553
(Linear) (Six-base) MAPSORT of: KIEE0553.seq Check: 7291 from: 1 to: 1071
KIEE0553.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1071
Size: 799 272
AflIII A'CryG_T
Cuts at: 0 828 1071
Size: 828 243
AleI CACnn'nnGTG
Cuts at: 0 829 967 1071
Size: 829 138 104
Fragments arranged by size:
829 138 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 222 253 1071
Size: 222 31 818
Fragments arranged by size:
818 222 31
ApaI G_GGCC'C
Cuts at: 0 918 1071
Size: 918 153
ApoI r'AATT_y
Cuts at: 0 316 454 517 1071
Size: 316 138 63 554
Fragments arranged by size:
554 316 138 63
BamHI G'GATC_C
Cuts at: 0 579 1071
Size: 579 492
BanI G'GyrC_C
Cuts at: 0 632 676 1071
Size: 632 44 395
Fragments arranged by size:
632 395 44
BanII G_rGCy'C
Cuts at: 0 429 772 918 1071
Size: 429 343 146 153
Fragments arranged by size:
429 343 153 146
BbeI G_GCGC'C
Cuts at: 0 636 1071
Size: 636 435
BciVI GTATCCnnnnn_n'
Cuts at: 0 285 1071
Size: 285 786
BfrBI ATG'CAT
Cuts at: 0 535 1071
Size: 535 536
BglII A'GATC_T
Cuts at: 0 737 1071
Size: 737 334
Bme1580I G_kGCm'C
Cuts at: 0 918 1071
Size: 918 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 1071
Size: 387 684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1039 1071
Size: 786 253 32
Fragments arranged by size:
786 253 32
BsaAI yAC'GTr
Cuts at: 0 831 1071
Size: 831 240
BsaHI Gr'CG_yC
Cuts at: 0 633 1071
Size: 633 438
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 416 446 1071
Size: 19 30 367 30 625
Fragments arranged by size:
625 367 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1027 1071
Size: 1027 44
BseYI C'CCAG_C
Cuts at: 0 467 910 956 1071
Size: 467 443 46 115
Fragments arranged by size:
467 443 115 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1071
Size: 526 545
BsiHKAI G_wGCw'C
Cuts at: 0 332 1019 1071
Size: 332 687 52
Fragments arranged by size:
687 332 52
BsmI GAATG_Cn'
Cuts at: 0 535 1071
Size: 535 536
Bsp1286I G_dGCh'C
Cuts at: 0 332 429 772 918 1019 1071
Size: 332 97 343 146 101 52
Fragments arranged by size:
343 332 146 101 97 52
BspHI T'CATG_A
Cuts at: 0 929 1071
Size: 929 142
BsrBI CCG'CTC
Cuts at: 0 30 134 1071
Size: 30 104 937
Fragments arranged by size:
937 104 30
BsrGI T'GTAC_A
Cuts at: 0 482 1071
Size: 482 589
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 900 1071
Size: 504 396 171
Fragments arranged by size:
504 396 171
BstYI r'GATC_y
Cuts at: 0 579 737 1071
Size: 579 158 334
Fragments arranged by size:
579 334 158
DrdI GACnn_nn'nnGTC
Cuts at: 0 157 619 1071
Size: 157 462 452
Fragments arranged by size:
462 452 157
EaeI y'GGCC_r
Cuts at: 0 311 1071
Size: 311 760
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 345 1071
Size: 345 726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 799 1039 1071
Size: 786 13 240 32
Fragments arranged by size:
786 240 32 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 880 1071
Size: 880 191
EcoO109I rG'GnC_Cy
Cuts at: 0 684 914 1071
Size: 684 230 157
Fragments arranged by size:
684 230 157
EcoRI G'AATT_C
Cuts at: 0 316 1071
Size: 316 755
FspI TGC'GCA
Cuts at: 0 841 1071
Size: 841 230
HaeII r_GCGC'y
Cuts at: 0 403 636 666 892 1071
Size: 403 233 30 226 179
Fragments arranged by size:
403 233 226 179 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 827 859 1071
Size: 3 32 792 32 212
Fragments arranged by size:
792 212 32 32 3
KasI G'GCGC_C
Cuts at: 0 632 1071
Size: 632 439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 704 1071
Size: 67 637 367
Fragments arranged by size:
637 367 67
MslI CAynn'nnrTG
Cuts at: 0 335 829 967 1071
Size: 335 494 138 104
Fragments arranged by size:
494 335 138 104
MspA1I CmG'CkG
Cuts at: 0 237 1071
Size: 237 834
NarI GG'CG_CC
Cuts at: 0 633 1071
Size: 633 438
NsiI A_TGCA'T
Cuts at: 0 537 1071
Size: 537 534
PasI CC'CwG_GG
Cuts at: 0 998 1071
Size: 998 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 1071
Size: 702 369
PfoI T'CCnGG_A
Cuts at: 0 277 763 1071
Size: 277 486 308
Fragments arranged by size:
486 308 277
PmlI CAC'GTG
Cuts at: 0 831 1071
Size: 831 240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 1071
Size: 62 32 977
Fragments arranged by size:
977 62 32
PpuMI rG'GwC_Cy
Cuts at: 0 684 1071
Size: 684 387
PspOMI G'GGCC_C
Cuts at: 0 914 1071
Size: 914 157
PstI C_TGCA'G
Cuts at: 0 409 1071
Size: 409 662
PvuII CAG'CTG
Cuts at: 0 237 1071
Size: 237 834
ScaI AGT'ACT
Cuts at: 0 952 1071
Size: 952 119
SexAI A'CCwGG_T
Cuts at: 0 265 1071
Size: 265 806
SfcI C'TryA_G
Cuts at: 0 405 741 1071
Size: 405 336 330
Fragments arranged by size:
405 336 330
SfoI GGC'GCC
Cuts at: 0 634 1071
Size: 634 437
StyI C'CwwG_G
Cuts at: 0 508 1071
Size: 508 563
TatI w'GTAC_w
Cuts at: 0 482 950 1071
Size: 482 468 121
Fragments arranged by size:
482 468 121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1071
Size: 416 32 623
Fragments arranged by size:
623 416 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1000 1030 1071
Size: 1000 30 41
Fragments arranged by size:
1000 41 30
Enzymes that do cut:
AcuI AflIII AleI AlfI ApaI ApoI BamHI BanI
BanII BbeI BciVI BfrBI BglII Bme1580I BmrI BpmI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrBI BsrGI BstAPI BstYI DrdI EaeI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
KasI MmeI MslI MspA1I NarI NsiI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII ScaI
SexAI SfcI SfoI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbsI BbvCI BcgI BclI BglI BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI
BsiEI BsiWI BsmBI BspEI BspMI BsrDI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII EagI EarI EcoICRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PciI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI