(Circular) (Six-base) MAPSORT of: pFN21AE0485.seq Check: 8962 from: 1 to: 5302 pFN21AE0485 5302 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3112 278 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 1937 Size: 708 4594 Acc65I G'GTAC_C Cuts at: 2623 2623 Size: 5302 AclI AA'CG_TT Cuts at: 1563 2601 3429 3802 1563 Size: 1038 828 373 3063 Fragments arranged by size: 3063 1038 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 3365 4749 5017 1632 Size: 698 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 698 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5105 AflIII A'CryG_T Cuts at: 1203 2359 4201 5178 1203 Size: 1156 1842 977 1327 Fragments arranged by size: 1842 1327 1156 977 AgeI A'CCGG_T Cuts at: 4133 4133 Size: 5302 AhdI GACnn_n'nnGTC Cuts at: 4031 4031 Size: 5302 AleI CACnn'nnGTG Cuts at: 1166 2360 2498 1166 Size: 1194 138 3970 Fragments arranged by size: 3970 1194 138 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5302 AlwNI CAG_nnn'CTG Cuts at: 3045 4617 3045 Size: 1572 3730 ApaI G_GGCC'C Cuts at: 2031 2449 2031 Size: 418 4884 ApaLI G'TGCA_C Cuts at: 3359 4515 3359 Size: 1156 4146 ApoI r'AATT_y Cuts at: 1493 2474 2611 2837 2989 5098 1493 Size: 981 137 226 152 2109 1697 Fragments arranged by size: 2109 1697 981 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 3856 160 Size: 872 2824 1606 Fragments arranged by size: 2824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 AvaI C'yCGr_G Cuts at: 1870 1943 2627 1870 Size: 73 684 4545 Fragments arranged by size: 4545 684 73 BamHI G'GATC_C Cuts at: 2044 2632 2999 2044 Size: 588 367 4347 Fragments arranged by size: 4347 588 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2132 2623 4078 618 Size: 325 217 627 345 491 1455 1842 Fragments arranged by size: 1842 1455 627 491 345 325 217 BanII G_rGCy'C Cuts at: 1391 2031 2303 2449 2621 1391 Size: 640 272 146 172 4072 Fragments arranged by size: 4072 640 272 172 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4331 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3493 3527 1903 Size: 34 1556 34 3678 Fragments arranged by size: 3678 1556 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3194 4404 3194 Size: 1210 4092 BclI T'GATC_A Cuts at: 1548 1548 Size: 5302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3913 136 Size: 107 122 71 631 605 2241 1525 Fragments arranged by size: 2241 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2268 5297 2268 Size: 3029 2273 BlpI GC'TnA_GC Cuts at: 2719 2719 Size: 5302 Bme1580I G_kGCm'C Cuts at: 1790 2031 2449 3363 4519 1790 Size: 241 418 914 1156 2573 Fragments arranged by size: 2573 1156 914 418 241 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5302 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3033 3991 442 Size: 416 53 83 399 24 1616 958 1753 Fragments arranged by size: 1753 1616 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2317 2570 3962 1239 Size: 1078 253 1392 2579 Fragments arranged by size: 2579 1392 1078 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3427 4292 4590 3427 Size: 865 298 4139 Fragments arranged by size: 4139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 882 Size: 617 2466 2219 Fragments arranged by size: 2466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2362 3093 493 Size: 711 1158 731 2702 Fragments arranged by size: 2702 1158 731 711 BsaBI GATnn'nnATC Cuts at: 5296 5296 Size: 5302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3109 3491 4126 275 Size: 53 83 186 936 1576 382 635 1451 Fragments arranged by size: 1576 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3735 4133 4407 4554 4965 3735 Size: 398 274 147 411 4072 Fragments arranged by size: 4072 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5077 Fragments arranged by size: 5077 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5302 BseYI C'CCAG_C Cuts at: 2017 2441 2487 4505 5001 2017 Size: 424 46 2018 496 2318 Fragments arranged by size: 2318 2018 496 424 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4729 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2209 2752 3513 4541 1540 Size: 275 186 208 543 761 1028 2301 Fragments arranged by size: 2301 1028 761 543 275 208 186 BsiHKAI G_wGCw'C Cuts at: 2550 3363 3448 4519 2550 Size: 813 85 1071 3333 Fragments arranged by size: 3333 1071 813 85 BsmI GAATG_Cn' Cuts at: 2813 2906 2813 Size: 93 5209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2303 2449 2550 2621 3363 3448 Size: 399 241 272 146 101 71 742 85 Cuts at: 3448 4519 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 272 241 146 101 85 71 BspEI T'CCGG_A Cuts at: 4965 4965 Size: 5302 BspHI T'CATG_A Cuts at: 2460 3191 4921 2460 Size: 731 1730 2841 Fragments arranged by size: 2841 1730 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2657 844 Size: 1813 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3189 1394 Size: 429 1366 3507 Fragments arranged by size: 3507 1366 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3797 3971 65 Size: 1183 2549 174 1396 Fragments arranged by size: 2549 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3946 4133 1700 Size: 110 2136 187 2869 Fragments arranged by size: 2869 2136 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5302 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5302 BssSI C'ACGA_G Cuts at: 3362 4374 3362 Size: 1012 4290 BstAPI GCAn_nnn'nTGC Cuts at: 794 2431 794 Size: 1637 3665 BstBI TT'CG_AA Cuts at: 4106 4106 Size: 5302 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5302 BstYI r'GATC_y Cuts at: 1972 2044 2268 2632 2999 3384 3401 4853 4939 Size: 72 224 364 367 385 17 1452 86 Cuts at: 4939 4951 5297 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 364 346 224 86 72 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3811 Fragments arranged by size: 3811 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4563 Fragments arranged by size: 4563 683 56 BtsI GCAGTG_nn' Cuts at: 2420 2482 2818 3612 3632 2420 Size: 62 336 794 20 4090 Fragments arranged by size: 4090 794 336 62 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5267 DraI TTT'AAA Cuts at: 2606 2606 Size: 5302 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4309 809 Size: 507 2993 1802 Fragments arranged by size: 2993 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2749 3638 Size: 54 1425 50 275 18 376 543 889 Cuts at: 3638 5267 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 543 376 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2206 2749 1537 Size: 275 394 543 4090 Fragments arranged by size: 4090 543 394 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3232 1353 Size: 1879 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3889 4263 4409 387 Size: 1064 138 2300 374 146 1280 Fragments arranged by size: 2300 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2317 2330 2570 3365 3962 4749 5017 Size: 393 685 13 240 795 597 787 268 Cuts at: 5017 1239 Size: 1524 Fragments arranged by size: 1524 795 787 685 597 393 268 240 13 EcoNI CCTnn'n_nnAGG Cuts at: 2411 2411 Size: 5302 EcoO109I rG'GnC_Cy Cuts at: 2445 2445 Size: 5302 EcoRI G'AATT_C Cuts at: 2611 2989 2611 Size: 378 4924 FspI TGC'GCA Cuts at: 3808 3808 Size: 5302 HaeII r_GCGC'y Cuts at: 1678 2122 4449 1678 Size: 444 2327 2531 Fragments arranged by size: 2531 2327 444 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2358 2390 3943 3975 4017 Size: 32 135 32 786 32 1553 32 42 Cuts at: 4017 4049 1373 Size: 32 2626 Fragments arranged by size: 2626 1553 786 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2646 2892 669 Size: 988 281 708 246 3079 Fragments arranged by size: 3079 988 708 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5302 HpaI GTT'AAC Cuts at: 2892 2892 Size: 5302 KpnI G_GTAC'C Cuts at: 2627 2627 Size: 5302 MfeI C'AATT_G Cuts at: 2901 2901 Size: 5302 MluI A'CGCG_T Cuts at: 5178 5178 Size: 5302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2088 2235 4416 4600 1305 Size: 783 147 2181 184 2007 Fragments arranged by size: 2181 2007 783 184 147 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3480 Fragments arranged by size: 3480 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2498 3260 3619 3778 518 Size: 648 1194 138 762 359 159 2042 Fragments arranged by size: 2042 1194 762 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2718 3395 4543 4788 1606 Size: 1112 677 1148 245 2120 Fragments arranged by size: 2120 1148 1112 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3811 Fragments arranged by size: 3811 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5302 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5302 NotI GC'GGCC_GC Cuts at: 2749 2749 Size: 5302 NspI r_CATG'y Cuts at: 1132 1229 2660 4205 1132 Size: 97 1431 1545 2229 Fragments arranged by size: 2229 1545 1431 97 PasI CC'CwG_GG Cuts at: 1348 2529 1348 Size: 1181 4121 PciI A'CATG_T Cuts at: 4201 4201 Size: 5302 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5302 PfoI T'CCnGG_A Cuts at: 2294 2294 Size: 5302 PmeI GTTT'AAAC Cuts at: 2606 2606 Size: 5302 PmlI CAC'GTG Cuts at: 2362 2362 Size: 5302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3371 3403 4884 4916 3371 Size: 32 1481 32 3757 Fragments arranged by size: 3757 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5302 PsiI TTA'TAA Cuts at: 2872 2872 Size: 5302 PspOMI G'GGCC_C Cuts at: 2027 2445 2027 Size: 418 4884 PstI C_TGCA'G Cuts at: 830 2654 830 Size: 1824 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5302 SalI G'TCGA_C Cuts at: 1936 2644 1936 Size: 708 4594 SbfI CC_TGCA'GG Cuts at: 2654 2654 Size: 5302 ScaI AGT'ACT Cuts at: 3550 5262 3550 Size: 1712 3590 SexAI A'CCwGG_T Cuts at: 2235 2235 Size: 5302 SfcI C'TryA_G Cuts at: 826 1045 2272 2650 3785 4466 4657 826 Size: 219 1227 378 1135 681 191 1471 Fragments arranged by size: 1471 1227 1135 681 378 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5302 SmaI CCC'GGG Cuts at: 1872 2629 1872 Size: 757 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3406 4307 4569 820 Size: 197 926 1463 901 262 1553 Fragments arranged by size: 1553 1463 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3093 493 Size: 2600 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5302 SphI G_CATG'C Cuts at: 1132 2660 1132 Size: 1528 3774 SspI AAT'ATT Cuts at: 5 52 3226 5 Size: 47 3174 2081 Fragments arranged by size: 3174 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5302 StyI C'CwwG_G Cuts at: 513 1065 2004 2741 513 Size: 552 939 737 3074 Fragments arranged by size: 3074 939 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2128 3329 3346 3499 3684 4129 325 Size: 1255 548 1201 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1201 548 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3548 5260 96 Size: 274 80 33 51 1443 1571 1712 138 Fragments arranged by size: 1712 1571 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5262 5294 5262 Size: 32 5270 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5302 XbaI T'CTAG_A Cuts at: 2638 2638 Size: 5302 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2531 2561 2038 Size: 493 30 4779 Fragments arranged by size: 4779 493 30 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5302 XmaI C'CCGG_G Cuts at: 1870 2627 1870 Size: 757 4545 XmnI GAAnn'nnTTC Cuts at: 3431 3431 Size: 5302 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3110 276 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI