################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HgaI HindIII HpaI KasI MfeI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI SalI SapI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI StyI SwaI TfiI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 520 GACGTC GACGT^C Acc65I 1241 GGTACC G^GTACC AccI 16 GTCTAC GT^[AC][GT]AC AccIII 1295 TCCGGA T^CCGGA AcyI 520 GACGTC G[GA]^CG[CT]C AlwNI 1246 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 1241 GGTACC G^GTACC AspI 682 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 605 TGCGCA TGC^GCA BanI 1241 GGTACC G^G[CT][GA]CC BcgI 608 GCAGCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 917 TGATCA T^GATCA BfaI 1124 CTAG C^TAG BsaAI 19 TACGTG [CT]AC^GT[GA] BsaBI 864 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 520 GACGTC G[GA]^CG[CT]C BsaWI 1295 TCCGGA [AT]^CCGG[AT] BseAI 1295 TCCGGA T^CCGGA BsgI 262 GTGCAG GTGCAG(16/14) BsmAI 839 GTCTC GTCTC(1/5) BsmBI 838 CGTCTC CGTCTC(1/5) BspEI 1295 TCCGGA T^CCGGA BspLU11I 77 ACATGT A^CATGT BspMI 264 GCAGGT ACCTGC(4/8) BsrDI 596 GCAATG GCAATG(2/0) BstAPI 93 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 478 CGGTCCG CG^G[AT]CCG DdeI 407 CTCAG C^T[ACGT]AG EaeI 442 CGGCCA [CT]^GGCC[GA] EarI 1233 GAAGAG CTCTTC(1/4) EcoNI 249 CCTCTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 917 TGATCA T^GATCA FspI 605 TGCGCA TGC^GCA Hin1I 520 GACGTC G[GA]^CG[CT]C KpnI 1241 GGTACC GGTAC^C Ksp632I 1233 GAAGAG CTCTTC(1/4) MaeI 1124 CTAG C^TAG MamI 864 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 221 ACGCGT A^CGCGT MroI 1295 TCCGGA T^CCGGA MseI 1031 TTAA T^TAA PflFI 682 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 417 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 391 CTGCAG CTGCA^G RsrII 478 CGGTCCG CG^G[AT]CCG SbfI 390 CCTGCAGG CCTGCA^GG ScaI 278 AGTACT AGT^ACT SfcI 391 CTGCAG C^T[GA][CT]AG SmaI 1080 CCCGGG CCC^GGG SphI 653 GCATGC GCATG^C Sse8387I 390 CCTGCAGG CCTGCA^GG Tru9I 1031 TTAA T^TAA Tth111I 682 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 417 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 556 CCAGGAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1080 CCCGGG C^CCGGG XmaI 1080 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AflIII 77 ACATGT A^C[GA][CT]GT 221 ACGCGT ApaI 434 GGGCCC GGGCC^C 1106 GGGCCC BbsI 1138 GAAGAC GAAGAC(2/6) 640 GTCTTC BpmI 292 CTGGAG CTGGAG(16/14) 733 CTGGAG BpuAI 1138 GAAGAC GAAGAC(2/6) 640 GTCTTC BsmFI 311 GGGAC GGGAC(10/14) 1039 GTCCC BsrI 753 CCAGT ACTGG(1/-1) 933 CCAGT BstXI 104 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 956 CCAACAACGTGG FauI 438 CCCGC CCCGC(4/6) 1104 GCGGG HaeII 10 AGCGCC [GA]GCGC^[CT] 586 AGCGCC HincII 535 GTCAAC GT[CT]^[GA]AC 948 GTCAAC HindII 535 GTCAAC GT[CT]^[GA]AC 948 GTCAAC KspI 439 CCGCGG CCGC^GG 708 CCGCGG MaeIII 457 GTCAC ^GT[ACGT]AC 575 GTCAC MspA1I 439 CCGCGG C[AC]G^C[GT]G 708 CCGCGG NspI 77 ACATGT [GA]CATG^[CT] 653 GCATGC PpuMI 686 AGGTCCT [GA]G^G[AT]CC[CT] 1037 GGGTCCC SacI 346 GAGCTC GAGCT^C 823 GAGCTC SacII 439 CCGCGG CCGC^GG 708 CCGCGG Tsp45I 457 GTCAC ^GT[GC]AC 575 GTCAC TspRI 754 CAGTG CA[GC]TG[ACGT][ACGT]^ 1164 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII DraII DsaI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I XhoII