(Circular) (Six-base) MAPSORT of: pFN21AB5347.seq Check: 2142 from: 1 to: 6010 pFN21AB5347 6010 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2260 2260 Size: 6010 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2529 3820 278 Size: 53 83 186 936 993 1291 2468 Fragments arranged by size: 2468 1291 993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2022 3353 1937 Size: 85 1331 4594 Fragments arranged by size: 4594 1331 85 Acc65I G'GTAC_C Cuts at: 3246 3331 3246 Size: 85 5925 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 3245 4073 5457 5725 1632 Size: 425 1188 828 1384 268 1917 Fragments arranged by size: 1917 1384 1188 828 425 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 2082 2226 4909 5886 1203 Size: 879 144 2683 977 1327 Fragments arranged by size: 2683 1327 977 879 144 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 2760 1166 Size: 1594 4416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2411 2624 3230 1184 Size: 1227 213 606 3964 Fragments arranged by size: 3964 1227 606 213 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2735 2767 2735 Size: 32 5978 AlwNI CAG_nnn'CTG Cuts at: 3256 3753 5325 3256 Size: 497 1572 3941 Fragments arranged by size: 3941 1572 497 ApaI G_GGCC'C Cuts at: 2443 3115 2443 Size: 672 5338 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 3319 3545 3697 5806 1493 Size: 1826 226 152 2109 1697 Fragments arranged by size: 2109 1826 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2653 2663 3047 3085 3335 1870 Size: 73 710 10 384 38 250 4545 Fragments arranged by size: 4545 710 384 250 73 38 10 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3246 3331 4786 618 Size: 325 217 627 1459 85 1455 1842 Fragments arranged by size: 1842 1459 1455 627 325 217 85 BanII G_rGCy'C Cuts at: 1391 2355 2443 2630 2832 3115 3329 1391 Size: 964 88 187 202 283 214 4072 Fragments arranged by size: 4072 964 283 214 202 187 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2638 3150 928 Size: 971 739 512 3788 Fragments arranged by size: 3788 971 739 512 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2602 2636 4201 4235 1903 Size: 34 665 34 1565 34 3678 Fragments arranged by size: 3678 1565 665 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 2922 1548 Size: 1374 4636 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4621 136 Size: 107 122 71 631 605 2949 1525 Fragments arranged by size: 2949 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6005 6005 Size: 6010 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2443 2991 3115 4071 5227 1790 Size: 653 548 124 956 1156 2573 Fragments arranged by size: 2573 1156 956 653 548 124 BmgBI CAC'GTC Cuts at: 1318 2077 2857 1318 Size: 759 780 4471 Fragments arranged by size: 4471 780 759 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3741 4699 442 Size: 416 53 83 399 24 2324 958 1753 Fragments arranged by size: 2324 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2666 2914 2666 Size: 248 5762 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 2759 4670 1239 Size: 1079 441 1911 2579 Fragments arranged by size: 2579 1911 1079 441 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4135 5000 5298 4135 Size: 865 298 4847 Fragments arranged by size: 4847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4673 882 Size: 617 3174 2219 Fragments arranged by size: 3174 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2026 3801 493 Size: 711 822 1775 2702 Fragments arranged by size: 2702 1775 822 711 BsaBI GATnn'nnATC Cuts at: 2873 6004 2873 Size: 3131 2879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2526 3817 4199 4834 Size: 53 83 186 936 993 1291 382 635 Cuts at: 4834 275 Size: 1451 Fragments arranged by size: 1451 1291 993 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3300 4443 4841 5115 5262 5673 3300 Size: 1143 398 274 147 411 3637 Fragments arranged by size: 3637 1143 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5785 Fragments arranged by size: 5785 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2144 2315 2318 1186 Size: 955 3 171 3 4878 Fragments arranged by size: 4878 955 171 3 3 BseYI C'CCAG_C Cuts at: 2575 5213 5709 2575 Size: 2638 496 2876 Fragments arranged by size: 2876 2638 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2288 1163 Size: 573 552 4885 Fragments arranged by size: 4885 573 552 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 2100 2355 2832 4071 4156 5227 2052 Size: 48 255 477 1239 85 1071 2835 Fragments arranged by size: 2835 1239 1071 477 255 85 48 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 BsmBI CGTCTCn'nnnn_ Cuts at: 2849 2849 Size: 6010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2100 2355 2443 2630 2832 2991 Size: 399 262 48 255 88 187 202 159 Cuts at: 2991 3115 3329 4071 4156 5227 1391 Size: 124 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 262 255 214 202 187 159 124 88 85 48 BspEI T'CCGG_A Cuts at: 3300 5673 3300 Size: 2373 3637 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2260 3365 844 Size: 1416 1105 3489 Fragments arranged by size: 3489 1416 1105 BsrBI CCG'CTC Cuts at: 1394 1823 3897 1394 Size: 429 2074 3507 Fragments arranged by size: 3507 2074 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2608 4505 4679 65 Size: 1183 1360 1897 174 1396 Fragments arranged by size: 1897 1396 1360 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4654 4841 1700 Size: 110 2844 187 2869 Fragments arranged by size: 2869 2844 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 794 Size: 1310 4700 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstXI CCAn_nnnn'nTGG Cuts at: 2116 2968 2116 Size: 852 5158 BstYI r'GATC_y Cuts at: 1972 2391 2973 3183 3340 3707 4092 4109 5561 Size: 419 582 210 157 367 385 17 1452 Cuts at: 5561 5647 5659 6005 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 582 419 385 367 346 210 157 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2068 2158 2444 2713 2730 513 Size: 552 1003 90 286 269 17 3793 Fragments arranged by size: 3793 1003 552 286 269 90 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 3526 4320 4340 3526 Size: 794 20 5196 Fragments arranged by size: 5196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5017 809 Size: 507 3701 1802 Fragments arranged by size: 3701 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2447 3457 4346 Size: 54 1425 50 275 18 617 1010 889 Cuts at: 4346 5975 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1010 889 617 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3233 3940 1353 Size: 1880 707 3423 Fragments arranged by size: 3423 1880 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 1999 4597 4971 5117 387 Size: 1064 138 410 2598 374 146 1280 Fragments arranged by size: 2598 1280 1064 410 374 146 138 EcoICRI GAG'CTC Cuts at: 2353 2830 2353 Size: 477 5533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2318 2759 3245 4073 4670 5457 Size: 393 425 261 441 486 828 597 787 Cuts at: 5457 5725 1239 Size: 268 1524 Fragments arranged by size: 1524 828 787 597 486 441 425 393 268 261 EcoNI CCTnn'n_nnAGG Cuts at: 2258 2258 Size: 6010 EcoO109I rG'GnC_Cy Cuts at: 2439 2440 2692 3043 3253 2439 Size: 1 252 351 210 5196 Fragments arranged by size: 5196 351 252 210 1 EcoRI G'AATT_C Cuts at: 3319 3697 3319 Size: 378 5632 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 6010 FspI TGC'GCA Cuts at: 2612 4516 2612 Size: 1904 4106 HaeII r_GCGC'y Cuts at: 1678 2019 2595 5157 1678 Size: 341 576 2562 2531 Fragments arranged by size: 2562 2531 576 341 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2819 2851 2987 3019 4651 Size: 32 135 32 1247 32 136 32 1632 Cuts at: 4651 4683 4725 4757 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1632 1247 136 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2542 2955 3354 3600 669 Size: 988 281 604 413 399 246 3079 Fragments arranged by size: 3079 988 604 413 399 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3250 3335 3250 Size: 85 5925 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 2226 5886 2226 Size: 3660 2350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5124 5308 1305 Size: 3819 184 2007 Fragments arranged by size: 3819 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4188 Fragments arranged by size: 4188 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2636 2760 2984 3212 3968 4327 Size: 648 948 522 124 224 228 756 359 Cuts at: 4327 4486 518 Size: 159 2042 Fragments arranged by size: 2042 948 756 648 522 359 228 224 159 124 MspA1I CmG'CkG Cuts at: 1606 2446 2715 3426 4103 5251 5496 1606 Size: 840 269 711 677 1148 245 2120 Fragments arranged by size: 2120 1148 840 711 677 269 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5458 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2086 2662 3368 4913 1132 Size: 97 857 576 706 1545 2229 Fragments arranged by size: 2229 1545 857 706 576 97 PasI CC'CwG_GG Cuts at: 1348 3261 1348 Size: 1913 4097 PciI A'CATG_T Cuts at: 2082 4909 2082 Size: 2827 3183 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 1108 Size: 1320 4690 PfoI T'CCnGG_A Cuts at: 2313 2313 Size: 6010 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2692 3043 2692 Size: 351 5659 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PspOMI G'GGCC_C Cuts at: 2439 3111 2439 Size: 672 5338 PstI C_TGCA'G Cuts at: 830 2400 3362 830 Size: 1570 962 3478 Fragments arranged by size: 3478 1570 962 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 RsrII CG'GwC_CG Cuts at: 2484 2484 Size: 6010 SacI G_AGCT'C Cuts at: 2355 2832 2355 Size: 477 5533 SacII CC_GC'GG Cuts at: 2447 2716 2447 Size: 269 5741 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SanDI GG'GwC_CC Cuts at: 3043 3043 Size: 6010 SbfI CC_TGCA'GG Cuts at: 2400 3362 2400 Size: 962 5048 ScaI AGT'ACT Cuts at: 2285 4258 5970 2285 Size: 1973 1712 2325 Fragments arranged by size: 2325 1973 1712 SfcI C'TryA_G Cuts at: 826 1045 2396 3358 4493 5174 5365 826 Size: 219 1351 962 1135 681 191 1471 Fragments arranged by size: 1471 1351 1135 962 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 3087 3337 1872 Size: 1215 250 4545 Fragments arranged by size: 4545 1215 250 SmlI C'TyrA_G Cuts at: 820 1017 1943 4114 5015 5277 820 Size: 197 926 2171 901 262 1553 Fragments arranged by size: 2171 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 2662 3368 1132 Size: 1530 706 3774 Fragments arranged by size: 3774 1530 706 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 3449 513 Size: 552 2384 3074 Fragments arranged by size: 3074 2384 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2867 3022 3253 4037 4054 4207 4392 Size: 1255 1287 155 231 784 17 153 185 Cuts at: 4392 4837 325 Size: 445 1498 Fragments arranged by size: 1498 1287 1255 784 445 231 185 155 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 2403 2916 Size: 274 80 33 51 1443 306 120 513 Cuts at: 2916 4256 5968 96 Size: 1340 1712 138 Fragments arranged by size: 1712 1443 1340 513 306 274 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2053 2085 5970 6002 2053 Size: 32 3885 32 2061 Fragments arranged by size: 3885 2061 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2690 1316 Size: 1374 4636 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2568 2568 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 3085 3335 1870 Size: 1215 250 4545 Fragments arranged by size: 4545 1215 250 XmnI GAAnn'nnTTC Cuts at: 4139 4139 Size: 6010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2527 3818 276 Size: 53 83 186 936 993 1291 2468 Fragments arranged by size: 2468 1291 993 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII SapI SexAI SfiI SfoI SrfI SwaI