##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     MaeI        MaeII       MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TfiI        
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccII         127  CGCG                 CG^CG
AcsI          235  AAATTT               [GA]^AATT[CT]
ApaI          226  GGGCCC               GGGCC^C
ApoI          235  AAATTT               [GA]^AATT[CT]
AvaII         205  GGACC                G^G[AT]CC
BanI           29  GGCACC               G^G[CT][GA]CC
BanII         226  GGGCCC               G[GA]GC[CT]^C
BmyI          226  GGGCCC               G[AGT]GC[ACT]^C
BpmI          376  CTGGAG               CTGGAG(16/14)
BsaI           73  GAGACC               GGTCTC(1/5)
BsaWI         179  TCCGGT               [AT]^CCGG[AT]
Bsp1286I      226  GGGCCC               G[AGT]GC[ACT]^C
BssSI         385  CACGAG               CACGAG(-5/-1)
BstF5I        309  CATCC                GGATG(2/0)
BstUI         127  CGCG                 CG^CG
DrdI          337  GACATCACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          126  CCGCGG               C^C[GA][CT]GG
EaeI          197  TGGCCG               [CT]^GGCC[GA]
FauI           77  CCCGC                CCCGC(4/6)
FokI          309  CATCC                GGATG(9/13)
HinfI         106  GAGTC                G^A[ACGT]TC
KspI          126  CCGCGG               CCGC^GG
MaeIII        211  GTCAC                ^GT[ACGT]AC
MseI          239  TTAA                 T^TAA
MslI          189  CACCAGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        126  CCGCGG               C[AC]G^C[GT]G
MvnI          127  CGCG                 CG^CG
NlaIII        150  CATG                 CATG^
PleI          106  GAGTC                GAGTC(4/5)
PpuMI         204  GGGACCT              [GA]G^G[AT]CC[CT]
SacII         126  CCGCGG               CCGC^GG
SfaNI         404  GCATC                GCATC(5/9)
Tru9I         239  TTAA                 T^TAA
Tsp45I        211  GTCAC                ^GT[GC]AC
Tsp509I       236  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI          380  AGCT                 AG^CT
              389  AGCT                
BcnI           21  CCGGG                CC^[GC]GG
              200  CCGGG               
BsgI           83  CTGCAC               GTGCAG(16/14)
              382  CTGCAC              
BsmAI          73  GAGAC                GTCTC(1/5)
              133  GTCTC               
BsmFI         204  GGGAC                GGGAC(10/14)
              302  GGGAC               
BstXI         149  CCATGAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              188  CCACCAGGGTGG        
DdeI           71  CTGAG                C^T[ACGT]AG
              173  CTGAG               
HphI          146  TCACC                GGTGA(8/7)
              212  TCACC               
NciI           21  CCGGG                CC^[GC]GG
              200  CCGGG               
StuI          324  AGGCCT               AGG^CCT
              362  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BssKI       BstNI       Cac8I       Cfr13I      DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      HaeIII      
HapII       HpaII       ItaI        MnlI        MspI        
MvaI        MwoI        NlaIV       Sau96I      ScrFI       
StyI        TseI